Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,327	A→G	intergenic (+770/‑874)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,327	0	A	G	100.0%	235.4 / NA	67	intergenic (+770/‑874)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/35);  total (32/35)

AAAACGAAGTGACTTTCCCACATGCCGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑ATCAATGACTGGCAGCAACAC  >  NC_000913/3951086‑3951556                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        aaaaCGAAGTGACTTTCCCACATGCCGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTcgcg                                                                                                                                                                                                                                >  2:98811/1‑251 (MQ=255)   aaCGAAGTGACTTTCCCACATGCCGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGt                                                                                                                                                                                                                              <  1:18937/251‑1 (MQ=255)        aGTGACTTTCCCACATGCCGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGAGAACTTCGCGGTGCAgg                                                                                                                                                                                                                         >  2:60405/1‑251 (MQ=255)         gtgacttcccatcatcccgagggtGAGCAAGCGGGCCAGTGGCGGGGAAAAGCGCAAAACCCGATGCTGTGCGTTGTGGGTGGCGTGGGTCTGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCGGGGAATGCATGGCGCCCAATCGGCGACCTTCGCGGTggagga                                                                                                                                                                                                                        <  2:2605/228‑1 (MQ=255)           gACTTTCCCACAGGCCGAAGTTGAGAAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAgt                                                                                                                                                                                                                      <  1:90047/251‑1 (MQ=255)           gACTTCCCCACATGCCGAAGTTGATCAAGCGCGCCAGGTGTGGGCAAAAGCGCAAAAACCGTTGCTGTGCGTTGGCGGTGGCGTGGGTATGGCGCAGGCATTTCCGGGTTTGCGTGAATTCCTCTCTGCCACAAAAATTCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAgt                                                                                                                                                                                                                      <  2:403150/251‑1 (MQ=255)                         ccGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCACAGCGGCGANCTTCGCGGGGCAGGAGTGCGACTTGCTGATc                                                                                                                                                                                                        >  2:232671/1‑251 (MQ=255)                           gAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGc                                                                                                                                                                                                      <  1:219810/251‑1 (MQ=255)                             aGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGATGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGACTTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCg                                                                                                                                                                                                    >  1:421244/1‑251 (MQ=255)                                  aGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGCGGCGTGGGGATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGCAGCAGATTATCCGTACTATCTGGGCATGCTGGGCATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGGGCGCCTTGCTGATCGCCGggggg                                                                                                                                                                                               >  2:57497/1‑250 (MQ=255)                                          ggcCAGATGATGGCAAAAGCGCAAATTCCGATGCGTTACTTTTGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGttt                                                                                                                                                                                       <  2:458851/250‑1 (MQ=255)                                               gATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTtgatg                                                                                                                                                                                  >  1:435061/1‑251 (MQ=255)                                                aTGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTtgatg                                                                                                                                                                                  >  1:377300/1‑250 (MQ=255)                                                   cTGGCAAAAGCGCAAAACCCGATGCTGTACGTTGGCGGCGGCGGGGGTATGGCGCAGGCAGTTCCGGCCTTGCGTGAATTTCGCGCTGCCACCCAAATGCCTGCCACCTGTACGCTGAACGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCAGGGCATGCTGGGCATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCATGGGTGCCCGTTTtgctggccg                                                                                                                                                                              >  2:269552/1‑244 (MQ=255)                                                      gCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGt                                                                                                                                                                           >  1:412032/1‑251 (MQ=255)                                                       cAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTg                                                                                                                                                                          <  2:92734/251‑1 (MQ=255)                                                         aaaGCGCAAAAACCTAGGCTGTACGTGGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTAGCCGTACTATCGGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGAc                                                                                                                                                                        <  2:281373/251‑1 (MQ=255)                                                           aGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCg                                                                                                                                                                      >  1:215738/1‑251 (MQ=255)                                                                 gcaaCCGATGCTGGACGTTGGCGGTGTCGTGTGTATGGCGCAGACAGTTCCGGCTTTGCGGGACTTTCTCGCTGCCCCACAAATGCCTGCCACCGGTCCGCTGAAAGGGCTGGGCGCAGACGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGTAGTGCGACTTGCTGATCACAGTGGAGGCCCGATTTGATGCACGGGTGACCGGCAAAc                                                                                                                                                                >  2:75876/3‑251 (MQ=255)                                                                 aaaaCCGATGCTGTACGTTGGCGGTGGCGTGGGTAGGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAAc                                                                                                                                                                <  2:412032/251‑1 (MQ=255)                                                                   aaCCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTg                                                                                                                                                              <  1:364525/251‑1 (MQ=255)                                                                     ccGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGCTGACCGGGTGACCGGCAAACTGac                                                                                                                                                            >  1:194380/1‑250 (MQ=255)                                                                        aTGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAacac                                                                                                                                                         <  1:111619/251‑1 (MQ=255)                                                                            cgtatgTTGGCGGTGGCGTGGGTATGGCGAAGGCAGTTCCGGCTTTGCGTTAAGTTCCCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCTGGTGACCGGCAAACTGAACACCTTc                                                                                                                                                     <  2:345605/246‑1 (MQ=255)                                                                             gTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTGTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTc                                                                                                                                                     <  1:411804/250‑1 (MQ=255)                                                                                          ggCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAg                                                                                                                                       <  1:304162/251‑1 (MQ=255)                                                                                                   aTGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTAGCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATcc                                                                                                                               <  2:200386/250‑1 (MQ=255)                                                                                                     ggCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGAGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCat                                                                                                                             >  1:227477/1‑250 (MQ=255)                                                                                                        gcaggcagTGCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTATAAGCAGATTATCCGTTCTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTTACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATgg                                                                                                                         <  1:207289/251‑1 (MQ=255)                                                                                                             cagTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATc                                                                                                                    >  2:393736/1‑251 (MQ=255)                                                                                                             cagTTACGTCTTTGCGTGTATTTCTCGCTGCTCCAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGCGCCTGCTGGGAAAGCATGGCACCAAATCGGCGAACTTCGCGGTACAGGACTGCGACTTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACAACTCCTCACCACACGCCCGCGTTATCCATATTGATATc                                                                                                                    >  2:383652/1‑251 (MQ=255)                                                                                                              atttCCGGTTTTGCGTGAATTTCTCGCGGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTTTCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGGACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCAACACACGCCAGTGTTATCCATATGGATATc                                                                                                                    <  2:445208/248‑1 (MQ=255)                                                                                                              agTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCg                                                                                                                   <  1:390966/251‑1 (MQ=255)                                                                                                                 tCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGGGCACGTTTTGATGACCGGGTGACCGGCAAACTGCACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGAcc                                                                                                                >  2:335061/1‑251 (MQ=255)                                                                                                                   cGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAACCTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCg                                                                                                              >  2:287213/1‑251 (MQ=255)                                                                                                                   cGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACNCGCCAGTGTTATCCATATGGAAATCAACCCg                                                                                                              >  2:227605/1‑251 (MQ=255)                                                                                                                          gCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGCTGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGGTATCCATATGGATATCGACCCGGCAGaa                                                                                                        >  2:7878/1‑250 (MQ=255)                                                                                                                           ggTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACGATCTGGGCAGGCTGGGAATGCATGGCACCAAAGCGGCGAACGTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCGTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAAt                                                                                                      <  2:156030/250‑1 (MQ=255)                                                                                                                                          gCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGt                                                                                         <  1:322677/249‑1 (MQ=255)                                                                                                                                              aaaaaaGGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGca                                                                                   <  2:337648/250‑1 (MQ=255)                                                                                                                                               aaaaaTGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGcac                                                                                  >  1:353319/1‑251 (MQ=255)                                                                                                                                                 aaaTGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACAt                                                                                <  1:374520/251‑1 (MQ=255)                                                                                                                                                     gcctgccACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACAtgtg                                                                             <  1:460478/250‑1 (MQ=255)                                                                                                                                                     gcctgccACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTgg                                                                            <  1:292475/251‑1 (MQ=255)                                                                                                                                                      cctgccACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGc                                                                           <  1:139405/251‑1 (MQ=255)                                                                                                                                                          ccACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTa                                                                       <  1:393736/251‑1 (MQ=255)                                                                                                                                                           cACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATNAACAAGCTGCGTCAGGCACATGTGGCATTAc                                                                      >  2:222899/1‑251 (MQ=255)                                                                                                                                                                  aCGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGa                                                               <  1:57497/251‑1 (MQ=255)                                                                                                                                                                    gcggAAAGGGCTGGGCGCAGTAGAAGCAGATNATCCGTACTATCGGGGCATGCTGGGAATGCATTGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACGTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGAtt                                                             <  2:234901/248‑1 (MQ=255)                                                                                                                                                                    gCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGAtt                                                             <  1:43222/251‑1 (MQ=255)                                                                                                                                                                       gAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGAGGCGAACTTAGCGGTGAAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGCGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCAGCGTCAGGCACATGTGGCATTACAAGGTGATTTa                                                           >  1:325911/1‑250 (MQ=255)                                                                                                                                                                         aaGGGCTGGGCGCAGTAGAAGCAGATTATCCGTCCTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTTAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAAt                                                        <  2:343674/251‑1 (MQ=255)                                                                                                                                                                           gggCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGc                                                      >  1:137377/1‑251 (MQ=255)                                                                                                                                                                                     cAGTAGAAGCAGATGATCCGTACTATCTGGGCATGCTGGGAAGGCATGGCACCTAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCAGGGGGTGCACGTTTTGATGACCGGGTGACAGGCAAACTGAACACCTTCGCACCACACGCCAGTGATATCCATATGGATATCGACCCGGCAGAAATGAACACGCTGCGTCAGGCACACGTGGCATTACAAGGTGCTTTAAATGCTCTGTTAcc                                             >  2:466475/1‑250 (MQ=255)                                                                                                                                                                                      aGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                             >  1:55947/1‑249 (MQ=255)                                                                                                                                                                                       gTAGAAGCAGATTATCCGTTCTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGc                                          <  2:4782/251‑1 (MQ=255)                                                                                                                                                                                             gCAGATTATCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAca                                    <  1:60405/251‑1 (MQ=255)                                                                                                                                                                                                    aTCCGTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCg                             >  2:377775/1‑251 (MQ=255)                                                                                                                                                                                                        gTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa                         <  1:29809/251‑1 (MQ=255)                                                                                                                                                                                                          aCTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑At                     >  1:417501/1‑251 (MQ=255)                                                                                                                                                                                                             aTCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatc                  >  1:353027/1‑248 (MQ=255)                                                                                                                                                                                                                 gggCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatcaatg              <  1:335061/251‑8 (MQ=255)                                                                                                                                                                                                                   gCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatcaatgac            <  1:280148/251‑10 (MQ=255)                                                                                                                                                                                                                       gCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATATCAATGACTGgc          >  1:24200/1‑251 (MQ=255)                                                                                                                                                                                                                          gggaaTGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATATCAATGACTGgcagc       >  1:428243/5‑251 (MQ=255)                                                                                                                                                                                                                             aaTGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATATCAATGACGGgcagctac    >  2:445178/2‑248 (MQ=255)                                                                                                                                                                                                                               tGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATATCAATGACTGGCAGCTacac  >  1:321977/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        AAAACGAAGTGACTTTCCCACATGCCGAAGTTGAGCAAGCGCGCCAGATGCTGGCAAAAGCGCAAAAACCGATGCTGTACGTTGGCGGTGGCGTGGGTATGGCGCAGGCAGTTCCGGCTTTGCGTGAATTTCTCGCTGCCACAAAAATGCCTGCCACCTGTACGCTGAAAGGGCTGGGCGCAGTAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑ATCAATGACTGGCAGCAACAC  >  NC_000913/3951086‑3951556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑