Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,893	A→C	A219A (GCT→GCG)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,893	0	A	C	100.0%	198.0 / NA	57	A219A (GCT→GCG)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/21);  total (36/21)

AACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACC  >  NC_000913/3815650‑3816139                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aaCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGCTTC‑CCCCTC‑GGGCCAGCGcccac                                                                                                                                                                                                                                                  >  2:439689/1‑248 (MQ=255)      cccGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAggt                                                                                                                                                                                                                                             >  1:209214/1‑249 (MQ=255)            gTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAg                                                                                                                                                                                                                                       <  1:378492/251‑1 (MQ=255)              tGAAGAAATAGGGGCTTTTGCGCCCGGATTTCCGCGTAACCTCGCCAAACTTTAACACCTGCGTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGc                                                                                                                                                                                                                                      <  2:46368/250‑1 (MQ=255)                aagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCACCAAGGCGAGTAGctct                                                                                                                                                                                                                                   >  2:271958/1‑251 (MQ=255)                 agaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGAGTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCtt                                                                                                                                                                                                                                  >  1:226294/1‑251 (MQ=255)                  gaaATAGGGGCTTTTGCGCCCGGCTTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTc                                                                                                                                                                                                                                 >  1:405135/1‑251 (MQ=255)                   aaaTAAGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAAAAAGGTGAGTAGCTCTTcc                                                                                                                                                                                                                                >  2:5418/1‑250 (MQ=255)                          ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGAGAGGAGCTCTTCATGGGTg                                                                                                                                                                                                                          >  2:434682/1‑250 (MQ=255)                                    cccGGATTTCAGCTTAAACTCGCCAAACGTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTGCAAGGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGGGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                                                                                                                                               <  1:88290/251‑1 (MQ=255)                                       ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCCACACGGTGAGTAGATCTTCATGGGGGAACGGCTCGCCTTc                                                                                                                                                                                                            >  2:106325/1‑251 (MQ=255)                                                       tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGCTTCATGCCTTCGCTCATCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTATGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt                                                                                                                                                                                            >  1:403419/1‑251 (MQ=255)                                                          ccAAACTTTAACCCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTTATATTGTTTCATGCCTTCGCTCCTCATCTTTCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCTCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCa                                                                                                                                                                                         <  1:107728/251‑1 (MQ=255)                                                               cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgat                                                                                                                                                                                    >  2:312043/1‑251 (MQ=255)                                                                    acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                                                                                                                                               >  1:409217/1‑251 (MQ=255)                                                                          gcttgctAAGCGCAAATTCAATAAACTTGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAGCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTc                                                                                                                                                                           <  2:436868/249‑1 (MQ=255)                                                                          gcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTc                                                                                                                                                                           <  1:192134/249‑1 (MQ=255)                                                                                 aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCACGGATGCGccctccttcgtccat                                                                                                                                                                  >  1:75426/1‑239 (MQ=255)                                                                                 aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATATTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                                                                                                                                                  >  1:70753/1‑251 (MQ=255)                                                                                        aaatCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGt                                                                                                                                                                                                                           <  2:31357/184‑1 (MQ=255)                                                                                        aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCCACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGAAGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGt                                                                                                                                                                                                                           >  1:31357/1‑187 (MQ=255)                                                                                        aaTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTGTCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCTTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATTGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTATCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                                                                                                           <  2:448488/251‑1 (MQ=255)                                                                                            cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGACGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                                                                                                                                                        >  2:192165/1‑250 (MQ=255)                                                                                                aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                                                                                                                                                  >  1:295634/1‑236 (MQ=255)                                                                                                aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCGCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                                                                                                                                                  <  2:295634/236‑1 (MQ=255)                                                                                                 aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGACGTCCCCTGCACTTCAATGATGAGCCCGTCTTCGGTCATCACTACGATCATGTCg                                                                                                                                                  >  1:432845/1‑251 (MQ=255)                                                                                                 aaCTCGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                                                                                                                                                  >  2:185063/1‑251 (MQ=255)                                                                                                      gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAAGGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtctc                                                                                                                                             <  1:365291/251‑1 (MQ=255)                                                                                                      gcgcTGATATGGTTTCATGCCTTCGCTCCTCAGCTTACTTGTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtctc                                                                                                                                             <  2:403419/251‑1 (MQ=255)                                                                                                             tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGct                                                                                                                                      >  2:425806/1‑250 (MQ=255)                                                                                                               tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCACCC‑TC‑GGGCCAGCGCCAACAAGGTGCGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGTCTCTGCGGctg                                                                                                                                     >  2:301/1‑248 (MQ=255)                                                                                                                 gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGt                                                                                                                                  <  1:312043/251‑1 (MQ=255)                                                                                                                       tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCa                                                                                                                             >  1:442918/1‑250 (MQ=255)                                                                                                                             cGCTCCTCATCTTACTTTACTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTAt                                                                                                                       <  2:169696/250‑1 (MQ=255)                                                                                                                                     aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATAAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTcc                                                                                                                    >  1:368202/1‑245 (MQ=255)                                                                                                                                           acagccTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaatt                                                                                                        <  1:413128/246‑3 (MQ=255)                                                                                                                                             tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaatt                                                                                                        <  1:185063/249‑3 (MQ=255)                                                                                                                                               tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCaaattgctt                                                                                                    >  1:103173/1‑245 (MQ=255)                                                                                                                                                     gACAAAAAAAAGGCGACTCATCAGTCGACTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGCTACAATGGATGCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTTAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGt                                                                                              <  1:266748/251‑1 (MQ=255)                                                                                                                                                       cAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTTAGTCGCTACAATGGATTCGATTC‑CCC‑CCCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGGGAACGGCTCGCCTTCTGCCGTCCACTGCACTTCAATGATGCGCCCGTCTTCGTTCATCACTACGTTCCTGTCGGTCTCTGAGGCTGAGTCCTCAACGGATTCCAGATCGCAAATTGCTTCACCGttt                                                                                            >  2:345905/1‑250 (MQ=255)                                                                                                                                                               aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAAtacc                                                                                    >  1:42203/1‑248 (MQ=255)                                                                                                                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGGGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGCTCGCAAATTGCTTCACCGTTTACAAtaccc                                                                                   >  2:324339/1‑247 (MQ=255)                                                                                                                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGAc                                                                                 >  2:122259/1‑249 (MQ=255)                                                                                                                                                                       catcaGTCGCCTTAAAAATCCGTTTGCCAGCGCCGCCTTCTGCGTCTCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATTGGTGACCGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTTCTTCACCGTTTACAATACCGACAGaa                                                                             <  1:128244/250‑1 (MQ=255)                                                                                                                                                                             tCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCgg                                                                      >  1:48131/1‑251 (MQ=255)                                                                                                                                                                                 cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTAc                                                                  >  2:314612/1‑251 (MQ=255)                                                                                                                                                                                   tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTAcc                                                                 <  1:356191/250‑1 (MQ=255)                                                                                                                                                                                               tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCg                                                    >  2:106038/1‑251 (MQ=255)                                                                                                                                                                                                 ccAGCGCCGCCTTCTGCGACGCTACAATGGATTCGCTTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGCCTTCGGTCATCACTACGTTCCTGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAACACCGACAGAAACTGCGGCTACCATCCCTTTCATCggg                                                  >  2:386509/1‑250 (MQ=255)                                                                                                                                                                                                      gccgccTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGt                                             >  1:293302/1‑251 (MQ=255)                                                                                                                                                                                                      gccgccTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGt                                             >  1:333844/1‑251 (MQ=255)                                                                                                                                                                                                         gccTTCTGCGTCGCTACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTCGCTATTCATGGGTGAACGGCTCGCCTTCAGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCCTCACTTCGTTCCTGTCGGTCTCTGCGGCTGGGTCTTCAACGTATTCCAGAGCGCCAATAGCTTCACCGGTTACAATACCGACAGAAACTGCGGACACCATCCCTTTCaccaggttgggttt                                          >  2:337481/1‑238 (MQ=255)                                                                                                                                                                                                                       tACAATGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGtttt                            >  2:128501/1‑251 (MQ=255)                                                                                                                                                                                                                            tGGATTCGATTC‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTccacc                       >  1:142970/1‑251 (MQ=255)                                                                                                                                                                                                                                 tcgattc‑CCCCTC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCt                   <  2:89455/250‑1 (MQ=255)                                                                                                                                                                                                                                   attcc‑ccc‑TC‑GGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATGCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTg               <  1:151864/247‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCTGAGTCTTCAACGTATTCCAGATCGCAAATTGCTTCACCGTTTACAATACCGACAGAAACTGCGGCTACCATCCCTTTCATCGGGTTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCtgcc  <  1:192165/251‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAGCTTGCCGTTTTCCACCAGCTTCTGTAGCGCATCTACC  >  NC_000913/3815650‑3816139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑