Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,634	A→G	D45D (GAT→GAC)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,634	0	A	G	100.0%	179.2 / NA	53	D45D (GAT→GAC)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/37);  total (16/37)

CATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA  >  NC_000913/3815393‑3815877                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGTCGCAATCGGGATCCCTTTGGTAACCGGGGCCAC‑CAGCAGGTCGGACTCCATTCCAGAATCCAACCATGGCT‑CAGCGTAAAAAAGGCCCAACAC‑TGCCAGGTCGGGcccc                                                                                                                                                                                                                                            >  1:253718/1‑250 (MQ=255) catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCAATCGGGGTCCCCTTG‑TAAGGCGGGCCCAACAGCCGATCGGACACCATGCCAAAATCCA‑CCAATGCTTCAACGGAAAAACGGCCTAACAG‑GGCCCGGGCGCGCCCg                                                                                                                                                                                                                                            >  1:101675/1‑251 (MQ=255)    catcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGt                                                                                                                                                                                                                                          <  1:291383/250‑1 (MQ=255)         aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGTCCTAACAG‑TGCCAGGTCGCGCCCGGTAt                                                                                                                                                                                                                                        <  1:373636/247‑1 (MQ=255)         aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt                                                                                                                                                                                                                                       <  1:46016/248‑1 (MQ=255)         aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt                                                                                                                                                                                                                                       <  2:434993/248‑1 (MQ=255)         aCCAGCATTACGCGTCCTGGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCATAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAt                                                                                                                                                                                                                                        <  1:88163/247‑1 (MQ=255)             gCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGc                                                                                                                                                                                                                                <  1:282872/251‑1 (MQ=255)                 tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCg                                                                                                                                                                                                                             <  1:243618/250‑1 (MQ=255)                                      accaaccaGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTCCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtt                                                                                                                                                                                                        <  2:296541/250‑1 (MQ=255)                                      accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAATCAGTCCGGCAGTTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGGCCAAACATCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTCCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGTCGTTGAAGAAATAGGGGCt                                                                                                                                                                                                         <  2:432589/249‑1 (MQ=255)                                      accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt                                                                                                                                                                                                         <  1:335850/249‑1 (MQ=255)                                           ccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgcgc                                                                                                                                                                                                  <  1:432612/251‑1 (MQ=255)                                                         gtaCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCATGGTCATTATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGGCCAAACAGCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGTCTCTTGCGCCCGGATTTCAGc                                                                                                                                                                                      <  2:382223/247‑1 (MQ=255)                                                                ggatctttgcttatgggcgtttAAAGCAGTTCGGCAGGTCAAGGTAATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCATAACAG‑TGCCAGGTCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAAcgc                                                                                                                                                                              <  2:177027/230‑3 (MQ=255)                                                                     ttggcctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGcc                                                                                                                                                                           <  1:447506/242‑1 (MQ=255)                                                                      ttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGGCGCCATCGGGGTCCCCTTG‑TAAGCCGGGCCAAACCACCGATCGGAATCCATGGCAGAATCCA‑CCCACGCGTCCTCGTAAAAAACGCCTAACAGCTGCC‑GGCCGCGCCCGGGATTAAACAGCCCCGCGTTGCCGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGGAAAcccacccaac                                                                                                                                                                       >  2:240599/1‑242 (MQ=255)                                                                                  ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                                                                                                                                           <  1:367969/251‑1 (MQ=255)                                                                                  ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                                                                                                                                           <  1:327145/251‑1 (MQ=255)                                                                                     tAAAGCAGTACGGCCGGTCAATGTCAGGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt                                                                                                                                                         <  2:26589/250‑1 (MQ=255)                                                                                     tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt                                                                                                                                                                                                         <  2:424512/202‑1 (MQ=255)                                                                                     tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt                                                                                                                                                         <  1:223825/250‑1 (MQ=255)                                                                                     tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGGCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAAAAG‑TGCCAGGGCGCGCCCGGGATTAAACAGCCCGGCGGTGAAGAAATAGGGGCt                                                                                                                                                                                                         >  1:424512/1‑202 (MQ=255)                                                                                       aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct                                                                                                                                                      <  1:423298/251‑1 (MQ=255)                                                                                                 gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                                                                                                                            <  1:97570/251‑1 (MQ=255)                                                                                                   aggtcaaggtcaTGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCACTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                                                                                                                                          <  1:240599/251‑1 (MQ=255)                                                                                                    ggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGCAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                                                                                                                                          >  1:436868/1‑250 (MQ=255)                                                                                                                 aTGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGGACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGGAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCt                                                                                                                            >  1:230880/1‑251 (MQ=255)                                                                                                                        gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg                                                                                                                                                                                                                                                  <  2:237594/126‑1 (MQ=255)                                                                                                                        gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg                                                                                                                                                                                                                                                  >  1:237594/1‑126 (MQ=255)                                                                                                                               ccACGGCGGTAGTGGTCGCAATCGAGATCCCTTGG‑TCAGCAGGGCCAAACAGCAGATCGACCTCAATGCCAGAATCCC‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCagg                                                                                                              <  2:56136/251‑3 (MQ=255)                                                                                                                                   ggCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCtt                                                                                                          <  2:422325/250‑1 (MQ=255)                                                                                                                                      ggtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCAGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGc                                                                                                       >  1:403232/5‑251 (MQ=255)                                                                                                                                       gtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGct                                                                                                      <  1:322680/248‑1 (MQ=255)                                                                                                                                               cgCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATc                                                                                               <  1:240550/249‑1 (MQ=255)                                                                                                                                               cgCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACGCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCTTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAATCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCatcatc                                                                                               <  2:138404/249‑1 (MQ=255)                                                                                                                                                         aTCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                     >  1:382114/1‑250 (MQ=255)                                                                                                                                                                    aaGCAGGGCCAAACAGCAGATCGAACTCAATGCCAGAAGCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCACTCCTCATCTTACTTTTCTACAGACaaaa                                                                           <  2:292961/250‑1 (MQ=255)                                                                                                                                                                        aGGGCCAAACAGCAGATCGAACTCAATGCCANAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg                                                                      <  2:230880/251‑1 (MQ=255)                                                                                                                                                                        aGGGCCAAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTAAGCGTAAAAACGGCCTACCAG‑TGCCAGGTCGCCCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCCGCGTAAACTCGCCAAACTTTAAAACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATCTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCaaaaaaa                                                                        >  2:365291/1‑249 (MQ=255)                                                                                                                                                                                          gAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCt                                                    <  2:298604/251‑1 (MQ=255)                                                                                                                                                                                               cAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGGTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaa                                                >  2:102178/1‑250 (MQ=255)                                                                                                                                                                                                aaTGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaaa                                              <  2:363690/251‑1 (MQ=255)                                                                                                                                                                                                   gccagAATCCA‑CCAATGCTTCAGCGTAAAAANGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTTGCGCTGATATGGGTTCATGCCTTCGCTCCTCAGCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa                                           <  2:204825/247‑1 (MQ=255)                                                                                                                                                                                                   gccagAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                            >  2:378956/5‑250 (MQ=255)                                                                                                                                                                                                               ccaATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                >  1:169696/1‑251 (MQ=255)                                                                                                                                                                                                                        cAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTc                        <  1:375105/250‑1 (MQ=255)                                                                                                                                                                                                                          gcttAAAAACGCCCAAACAG‑TGCGAGGTCGCGCCATGTATTAAACAGCCCGGCGTTGAAGAAAAAGGGTCTTTAGCGCCCGTATTTCAGCGTAACCTCGCCAAACTTTAACACCTTCATGCTAGGCGCAAATTCAGTAAACTGGAGCTGATATGGTCTCATGCCTTCGCTCCTCATCTTGCTTTTCTACAGCCAAAAGAAAGGCGACGCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                     <  2:10542/248‑1 (MQ=255)                                                                                                                                                                                                                            gTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACACAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                   >  2:436470/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaaaCGGCCTACCAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCTGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAATCTGGCGCTGATATGGTTTCATGCCTTCTCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                  <  1:194126/250‑1 (MQ=255)                                                                                                                                                                                                                                      ccTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg         >  1:48915/1‑251 (MQ=255)                                                                                                                                                                                                                                            aG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg   >  2:97977/1‑251 (MQ=255)                                                                                                                                                                                                                                             g‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga  <  1:378956/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    CATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA  >  NC_000913/3815393‑3815877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑