| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,601 | C→T | A56A (GCG→GCA) | pyrE ← | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,601 | 0 | C | T | 98.0% | 162.3 / ‑5.6 | 51 | A56A (GCG→GCA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (14/34); minor base G (1/0); total (17/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCG > NC_000913/3815362‑3815847 | gattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCCTGATGCTCCGCCAGCGCCCCGGCGGGAGGGGTCGCCAACGGGGTCCCCTTT‑TAAGCAGGG‑CCCAAACA‑CAAATCGGACTTAACGCCCGAATCCA‑CCAAAGCTTccccgg > 2:97570/4‑245 (MQ=255)gattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGGGTGGGCGCCAACGGGATCCCTTTG‑TTAGGAGGG‑GC‑CAACAGCAGAACGCACTCCATTCCAGAAACCC‑CCAATGCTTCAGCgg > 1:434993/4‑249 (MQ=255) gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAggtc < 1:203251/251‑4 (MQ=255) gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTCGCAATCGGGATCCCTTTG‑TAAACAGGG‑CC‑AAACAGGCGATAGGACTCAAAGCCCGAATCCA‑CCCATGCTTCAGCGTAAAAACGGACTAACaaggccagggc > 1:363690/1‑241 (MQ=255) catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGTCGCAATCGGGATCCCTTTGGTAACCGGGG‑CC‑AC‑CAGCAGGTCGGACTCCATTCCAGAATCCAACCATGGCT‑CAGCGTAAAAAAGGCCCAACAC‑TGCCAGGTCGGGcccc > 1:253718/1‑250 (MQ=255) catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCAATCGGGGTCCCCTTG‑TAAGGCGGG‑CC‑CAACAGCCGATCGGACACCATGCCAAAATCCA‑CCAATGCTTCAACGGAAAAACGGCCTAACAG‑GGCCCGGGCGCGCCCg > 1:101675/1‑251 (MQ=255) catcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGt < 1:291383/250‑1 (MQ=255) atcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATTCTCCGCCAGCGCCACGGCGGTTGTGGTCGCAATACGGATCCCCTTG‑TAAGCAGGGGCC‑AACCA‑CCGATCGAAATCCATGCCAGAATCCA‑CCAATGCTTCAGCGGAAAAACCGCCTAACAG‑TGCCAggtccccccccggat > 1:292961/1‑237 (MQ=255) aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGTCCTAACAG‑TGCCAGGTCGCGCCCGGTAt < 1:373636/247‑1 (MQ=255) aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt < 2:434993/248‑1 (MQ=255) aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt < 1:46016/248‑1 (MQ=255) aCCAGCATTACGCGTCCTGGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCATAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAt < 1:88163/247‑1 (MQ=255) gCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGc < 1:282872/251‑1 (MQ=255) tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCg < 1:243618/250‑1 (MQ=255) accaaccaGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTCCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtt < 2:296541/250‑1 (MQ=255) accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAATCAGTCCGGCAGTTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGG‑CC‑AAACATCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTCCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGTCGTTGAAGAAATAGGGGCt < 2:432589/249‑1 (MQ=255) accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt < 1:335850/249‑1 (MQ=255) ccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgcgc < 1:432612/251‑1 (MQ=255) gtaCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCATGGTCATTATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGTCTCTTGCGCCCGGATTTCAGc < 2:382223/247‑1 (MQ=255) ggatctttgcttatgggcgtttAAAGCAGTTCGGCAGGTCAAGGTAATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCATAACAG‑TGCCAGGTCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAAcgc < 2:177027/230‑3 (MQ=255) ttggcctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGcc < 1:447506/242‑1 (MQ=255) ttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGGCGCCATCGGGGTCCCCTTG‑TAAGCCGGG‑CC‑AAACCACCGATCGGAATCCATGGCAGAATCCA‑CCCACGCGTCCTCGTAAAAAACGCCTAACAGCTGCC‑GGCCGCGCCCGGGATTAAACAGCCCCGCGTTGCCGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGGAAAcccacccaac > 2:240599/1‑242 (MQ=255) ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc < 1:367969/251‑1 (MQ=255) ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc < 1:327145/251‑1 (MQ=255) tAAAGCAGTACGGCCGGTCAATGTCAGGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt < 2:26589/250‑1 (MQ=255) tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt < 2:424512/202‑1 (MQ=255) tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt < 1:223825/250‑1 (MQ=255) tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGGCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAAAAG‑TGCCAGGGCGCGCCCGGGATTAAACAGCCCGGCGGTGAAGAAATAGGGGCt > 1:424512/1‑202 (MQ=255) aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct < 1:423298/251‑1 (MQ=255) gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt < 1:97570/251‑1 (MQ=255) aggtcaaggtcaTGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCACTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc < 1:240599/251‑1 (MQ=255) ggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGCAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc > 1:436868/1‑250 (MQ=255) aTGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGGACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGGAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCt > 1:230880/1‑251 (MQ=255) gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg > 1:237594/1‑126 (MQ=255) gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg < 2:237594/126‑1 (MQ=255) ccACGGCGGTAGTGGTCGCAATCGAGATCCCTTGG‑TCAGCAGGG‑CC‑AAACAGCAGATCGACCTCAATGCCAGAATCCC‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCagg < 2:56136/251‑3 (MQ=255) ggCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCtt < 2:422325/250‑1 (MQ=255) ggtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCAGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGc > 1:403232/5‑251 (MQ=255) gtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGct < 1:322680/248‑1 (MQ=255) cgCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATc < 1:240550/249‑1 (MQ=255) cgCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACGCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCTTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAATCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCatcatc < 2:138404/249‑1 (MQ=255) aTCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt > 1:382114/1‑250 (MQ=255) aaGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAAGCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCACTCCTCATCTTACTTTTCTACAGACaaaa < 2:292961/250‑1 (MQ=255) aGGG‑CC‑AAACAGCAGATCGAACTCAATGCCANAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg < 2:230880/251‑1 (MQ=255) aGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTAAGCGTAAAAACGGCCTACCAG‑TGCCAGGTCGCCCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCCGCGTAAACTCGCCAAACTTTAAAACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATCTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCaaaaaaa > 2:365291/1‑249 (MQ=255) gAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCt < 2:298604/251‑1 (MQ=255) cAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGGTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaa > 2:102178/1‑250 (MQ=255) aaTGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaaa < 2:363690/251‑1 (MQ=255) gccagAATCCA‑CCAATGCTTCAGCGTAAAAANGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTTGCGCTGATATGGGTTCATGCCTTCGCTCCTCAGCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCa < 2:204825/247‑1 (MQ=255) gccagAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc > 2:378956/5‑250 (MQ=255) ccaATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg > 1:169696/1‑251 (MQ=255) | GACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCG > NC_000913/3815362‑3815847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |