Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,535	G→C	A78A (GCC→GCG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,535	0	G	C	100.0%	123.8 / NA	40	A78A (GCC→GCG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/29);  total (11/29)

TCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCT  >  NC_000913/3815296‑3815777                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        tCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGa                                                                                                                                                                                                                                               <  2:43009/250‑1 (MQ=255)               cGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAg                                                                                                                                                                                                                               <  1:291027/251‑1 (MQ=255)                            ccAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAAcagcag                                                                                                                                                                                                                <  1:151919/251‑1 (MQ=255)                                                                   gattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCCTGATGCTCCGCCAGCGCCCCGGCGGGAGGGGTCGCCAACGGGGTCCCCTTT‑TAAGCAGGG‑CCCAAACA‑CAAATCGGACTTAACGCCCGAATCCA‑CCAAAGCTTccccgg                                                                                                                                                                         >  2:97570/4‑245 (MQ=255)                                                                   gattCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGGGTGGGCGCCAACGGGATCCCTTTG‑TTAGGAGGG‑GC‑CAACAGCAGAACGCACTCCATTCCAGAAACCC‑CCAATGCTTCAGCgg                                                                                                                                                                         >  1:434993/4‑249 (MQ=255)                                                                                           gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAggtc                                                                                                                                               <  1:203251/251‑4 (MQ=255)                                                                                           gTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTCGCAATCGGGATCCCTTTG‑TAAACAGGG‑CC‑AAACAGGCGATAGGACTCAAAGCCCGAATCCA‑CCCATGCTTCAGCGTAAAAACGGACTAACaaggccagggc                                                                                                                                                >  1:363690/1‑241 (MQ=255)                                                                                                  catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGTCGCAATCGGGATCCCTTTGGTAACCGGGG‑CC‑AC‑CAGCAGGTCGGACTCCATTCCAGAATCCAACCATGGCT‑CAGCGTAAAAAAGGCCCAACAC‑TGCCAGGTCGGGcccc                                                                                                                                        >  1:253718/1‑250 (MQ=255)                                                                                                  catcatCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCAATCGGGGTCCCCTTG‑TAAGGCGGG‑CC‑CAACAGCCGATCGGACACCATGCCAAAATCCA‑CCAATGCTTCAACGGAAAAACGGCCTAACAG‑GGCCCGGGCGCGCCCg                                                                                                                                        >  1:101675/1‑251 (MQ=255)                                                                                                     catcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGt                                                                                                                                      <  1:291383/250‑1 (MQ=255)                                                                                                      atcTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGGCATGATTCTCCGCCAGCGCCACGGCGGTTGTGGTCGCAATACGGATCCCCTTG‑TAAGCAGGGGCC‑AACCA‑CCGATCGAAATCCATGCCAGAATCCA‑CCAATGCTTCAGCGGAAAAACCGCCTAACAG‑TGCCAggtccccccccggat                                                                                                                                    >  1:292961/1‑237 (MQ=255)                                                                                                          aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGTCCTAACAG‑TGCCAGGTCGCGCCCGGTAt                                                                                                                                    <  1:373636/247‑1 (MQ=255)                                                                                                          aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt                                                                                                                                   <  2:434993/248‑1 (MQ=255)                                                                                                          aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAtt                                                                                                                                   <  1:46016/248‑1 (MQ=255)                                                                                                          aCCAGCATTACGCGTCCTGGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCATAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTAt                                                                                                                                    <  1:88163/247‑1 (MQ=255)                                                                                                              gCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGc                                                                                                                            <  1:282872/251‑1 (MQ=255)                                                                                                                  tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCg                                                                                                                         <  1:243618/250‑1 (MQ=255)                                                                                                                                       accaaccaGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTCCGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCtt                                                                                                    <  2:296541/250‑1 (MQ=255)                                                                                                                                       accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAATCAGTCCGGCAGTTCAAGGGCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGG‑CC‑AAACATCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTCCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGTCGTTGAAGAAATAGGGGCt                                                                                                     <  2:432589/249‑1 (MQ=255)                                                                                                                                       accaaccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt                                                                                                     <  1:335850/249‑1 (MQ=255)                                                                                                                                            ccaGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTgcgc                                                                                              <  1:432612/251‑1 (MQ=255)                                                                                                                                                          gtaCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCATGGTCATTATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGAGCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGAGCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGTCTCTTGCGCCCGGATTTCAGc                                                                                  <  2:382223/247‑1 (MQ=255)                                                                                                                                                                 ggatctttgcttatgggcgtttAAAGCAGTTCGGCAGGTCAAGGTAATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCATAACAG‑TGCCAGGTCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAAcgc                                                                          <  2:177027/230‑3 (MQ=255)                                                                                                                                                                      ttggcctcttTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGcc                                                                       <  1:447506/242‑1 (MQ=255)                                                                                                                                                                       ttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGGAGTGGGCGCCATCGGGGTCCCCTTG‑TAAGCCGGG‑CC‑AAACCACCGATCGGAATCCATGGCAGAATCCA‑CCCACGCGTCCTCGTAAAAAACGCCTAACAGCTGCC‑GGCCGCGCCCGGGATTAAACAGCCCCGCGTTGCCGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGGAAAcccacccaac                                                                   >  2:240599/1‑242 (MQ=255)                                                                                                                                                                                   ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                                       <  1:367969/251‑1 (MQ=255)                                                                                                                                                                                   ggTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgc                                                       <  1:327145/251‑1 (MQ=255)                                                                                                                                                                                      tAAAGCAGTACGGCCGGTCAATGTCAGGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt                                                     <  2:26589/250‑1 (MQ=255)                                                                                                                                                                                      tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCt                                                                                                     <  2:424512/202‑1 (MQ=255)                                                                                                                                                                                      tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgctt                                                     <  1:223825/250‑1 (MQ=255)                                                                                                                                                                                      tAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGGCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAAAAG‑TGCCAGGGCGCGCCCGGGATTAAACAGCCCGGCGGTGAAGAAATAGGGGCt                                                                                                     >  1:424512/1‑202 (MQ=255)                                                                                                                                                                                        aaGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCtgcttgct                                                  <  1:423298/251‑1 (MQ=255)                                                                                                                                                                                                  gCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAt                                        <  1:97570/251‑1 (MQ=255)                                                                                                                                                                                                    aggtcaaggtcaTGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCACTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                                      <  1:240599/251‑1 (MQ=255)                                                                                                                                                                                                     ggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGCAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTc                                      >  1:436868/1‑250 (MQ=255)                                                                                                                                                                                                                  aTGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGGACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGGAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCt                        >  1:230880/1‑251 (MQ=255)                                                                                                                                                                                                                         gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg                                                                                                                                              <  2:237594/126‑1 (MQ=255)                                                                                                                                                                                                                         gCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTcg                                                                                                                                              >  1:237594/1‑126 (MQ=255)                                                                                                                                                                                                                                ccACGGCGGTAGTGGTCGCAATCGAGATCCCTTGG‑TCAGCAGGG‑CC‑AAACAGCAGATCGACCTCAATGCCAGAATCCC‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCagg          <  2:56136/251‑3 (MQ=255)                                                                                                                                                                                                                                    ggCGGTAGTGGTCGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCtt      <  2:422325/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCAGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGc   >  1:403232/5‑251 (MQ=255)                                                                                                                                                                                                                                        gtagtggtcGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCAGAATCCA‑CCAATGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGct  <  1:322680/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                        TCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTG‑TAAGCAGGG‑CC‑AAACAGCAGATCGAACTCAATGCCGGAATCCA‑CCAACGCTTCAGCGTAAAAACGGCCTAACAG‑TGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCT  >  NC_000913/3815296‑3815777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑