Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	41 [0]	[0] 40	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	40 (0.700)	36/488	0.3	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

AATATAATAAGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC  >  NC_000913/3079157‑3079640                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 tttccaggtcgataaacagacgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatataggtattaatcaatatccgtggtttatccggtacaAAGCAAtt                                                                                                                                                                                                                                           >  1:167535‑M2/244‑251 (MQ=255)                acagacgctgtcagaaattacacccaccagtaattcattatcagtaatttctggcagttcaaatacgcgcagacggccatcccggttgccataaatagcagcaactttggttttcatttttacctctgtaagcagtgtttattttcggcggttaattgattaaataaagtgtcgagttttggatcgcccatatagttaataatcaatatcagtggtttatccgtcacaaagcaattgagctgtgaatccgt                                                                                                                                                                                                                            <  2:180974‑M2/23‑12 (MQ=255)                  agacgctgtcagaaattaaactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGgtg                                                                                                                                                                                                                          >  2:128314‑M2/227‑251 (MQ=255)                    acgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGtt                                                                                                                                                                                                                        >  2:417919‑M2/225‑251 (MQ=255)                     cgctgtcagaaattacactcaccagtaaatcattatcgggaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgttgagttttggatctccaatatagttattaagcaatatcagttgtttatccgtcacaAAGCAATTGAGCAGGGAATCCGGTGttt                                                                                                                                                                                                                       <  2:345303‑M2/28‑1 (MQ=255)                             gaaattacactcaccagtaattcattatcggtaatttctggcaggtcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtttttatgttcggcggttaattgattaaataaagtgttgagttttggatcgccaatatagttattaggcaatatcagttgtttatccgtcacaAAGCAATTGAGCAGGTAAACCGGTGTtttgaatgca                                                                                                                                                                                                               <  2:366201‑M2/36‑5 (MQ=255)                                attacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTg                                                                                                                                                                                                            <  2:162478‑M2/39‑1 (MQ=255)                                    cactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgctaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAatt                                                                                                                                                                                                        >  2:8574‑M2/209‑251 (MQ=255)                                         accagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAAttatta                                                                                                                                                                                                    >  1:46922‑M2/204‑250 (MQ=255)                                          ccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAt                                                                                                                                                                                                  >  2:369976‑M2/203‑251 (MQ=255)                                           cagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAtt                                                                                                                                                                                                 >  1:38676‑M2/202‑251 (MQ=255)                                            agtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAAttt                                                                                                                                                                                                <  2:144046‑M2/51‑1 (MQ=255)                                                         cgttaattgctggccgttcaagtacgggcagacggacatgccgcttgccgtaaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccggcaccAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTACTTTCTTAAAAAACAtt                                                                                                                                                                                   >  2:175660‑M2/188‑251 (MQ=255)                                                          ggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTg                                                                                                                                                                                  >  1:417003‑M2/187‑251 (MQ=255)                                                                  ctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGagtttt                                                                                                                                                                            >  2:161253‑M2/179‑247 (MQ=255)                                                                       agttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaataaatatcaggggtttatccgtaacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGatat                                                                                                                                                                      >  1:202561‑M2/174‑250 (MQ=255)                                                                        gttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgttgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATaa                                                                                                                                                                    <  1:170096‑M2/79‑1 (MQ=255)                                                                            aaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATaacaac                                                                                                                                                                <  1:20473‑M2/83‑1 (MQ=255)                                                                            aaatacgcgcagacggacatcccgcttgccataaatagaagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGATATAGATATaacaac                                                                                                                                                                >  1:354245‑M2/169‑251 (MQ=255)                                                                                        acggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATAc                                                                                                                                                    <  1:177476‑M2/95‑1 (MQ=255)                                                                                           gacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGGGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGc                                                                                                                                                  >  1:114144‑M2/154‑250 (MQ=255)                                                                                                    cttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAAACGGGGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGAt                                                                                                                                        >  2:313341‑M2/145‑251 (MQ=255)                                                                                                          ataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtaacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAAAAAATGTCAATACGCATATCGTGATGCatat                                                                                                                                  >  2:133047‑M2/139‑251 (MQ=255)                                                                                                            aaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTa                                                                                                                                <  1:348017‑M2/115‑1 (MQ=255)                                                                                                                agcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATa                                                                                                                            <  2:444172‑M2/119‑1 (MQ=255)                                                                                                                         tttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgcgttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATCTAACAACAAATGTCAATACGNATATCGTGATGCATATTACATAATTTAtatt                                                                                                                   >  2:225894‑M2/124‑251 (MQ=255)                                                                                                                                   atttttaacgctggaatcagtgtttatgttctgcggttaattgattaaataaagtgtcgagttttggatcgacaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCatat                                                                                                         <  2:136606‑M2/138‑1 (MQ=255)                                                                                                                                         acctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAatat                                                                                                   <  1:174091‑M2/144‑1 (MQ=255)                                                                                                                                                 aatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGAt                                                                                            <  2:369426‑M2/151‑1 (MQ=255)                                                                                                                                                     agtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatttagttatttatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATTAAAATATTTGTGAtctta                                                                                        >  2:289609‑M2/96‑248 (MQ=255)                                                                                                                                                        ttttatgttcgacggttaattgattaaataaagtgtcgagttttggatcgccaatataggtattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATTTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTTATCTacaac                                                                                     <  2:151635‑M2/158‑1 (MQ=255)                                                                                                                                                                 cggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTAt                                                                            <  2:417003‑M2/167‑1 (MQ=255)                                                                                                                                                                                   ataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTCTCTATTGCTTGTCcgcacac                                                         >  2:414662‑M2/66‑248 (MQ=255)                                                                                                                                                                                           tcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACatat                                                 >  1:12993‑M2/58‑251 (MQ=255)                                                                                                                                                                                                      acctccaatataggtattaatcaatagaagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACTCATATCGTGATGCATATTACATAATTTATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGCCCGCAAACGGACATATCAAGGtaataa                                      <  2:91726‑M2/205‑1 (MQ=255)                                                                                                                                                                                                                 agttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATTCGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACGCGCCTTATCTATCGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGcc                           <  1:427310‑M2/216‑1 (MQ=255)                                                                                                                                                                                                                            aatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                <  2:22578‑M2/227‑1 (MQ=255)                                                                                                                                                                                                                                          atccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGTCCTTTTTACCCGAAATGc  <  2:178801‑M2/241‑1 (MQ=255)                                                                                                                                                                                                                                          atccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc  >  1:250444‑M2/11‑251 (MQ=255)                                                                                                                                                                                                                                          atccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc  <  1:133047‑M2/241‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 AATATAATAAGGGCCATTGTTAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTTTTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC  >  NC_000913/3079157‑3079640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑