Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,537 +AT intergenic (+980/‑664) ilvX → / → ilvM uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5351.A92.1% 218.0 / 12.8 63intergenic (+978/‑666)ilvX/ilvMuncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base A (32/26);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01
*NC_0009133,951,5352.T92.1% 218.0 / 12.1 63intergenic (+978/‑666)ilvX/ilvMuncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base T (32/26);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGG  >  NC_000913/3951285‑3951774
                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                  
gTACTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa                                                                                                                                                                                                                                                      <  1:29809/251‑1 (MQ=255)
  aCTATCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑At                                                                                                                                                                                                                                                  >  1:417501/1‑251 (MQ=255)
     aTCTGGGCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatc                                                                                                                                                                                                                                               >  1:353027/1‑248 (MQ=255)
         gggCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatcaatg                                                                                                                                                                                                                                           <  1:335061/251‑8 (MQ=255)
           gCATGCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑Atatcaatgac                                                                                                                                                                                                                                         <  1:280148/251‑10 (MQ=255)
               gCTGGGAATGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGgc                                                                                                                                                                                                                                     >  1:24200/1‑251 (MQ=255)
                  gggaaTGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGgcagc                                                                                                                                                                                                                                  >  1:428243/5‑251 (MQ=255)
                     aaTGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACGGgcagctac                                                                                                                                                                                                                               >  2:445178/2‑248 (MQ=255)
                       tGCATGGCACCAAAGCGGCGAACTTCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTacac                                                                                                                                                                                                                             >  1:321977/1‑251 (MQ=255)
                                              ttCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCAAACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAAc                                                                                                                                                                                                       >  1:179799/1‑250 (MQ=255)
                                               tCGCGGTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACa                                                                                                                                                                                                      <  1:377775/250‑1 (MQ=255)
                                                   ggTGCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAAcctgcct                                                                                                                                                                                                 >  1:326027/1‑245 (MQ=255)
                                                      gCAGGAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGCGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGAACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGc                                                                                                                                                                                              >  1:72290/1‑251 (MQ=255)
                                                          gAGTGCGACTTGCTGATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTa                                                                                                                                                                                          <  1:253609/251‑1 (MQ=255)
                                                            gtgcgacttgctgATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACg                                                                                                                                                                                        >  2:373581/9‑251 (MQ=255)
                                                              gcgacttgctgATCGCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGAc                                                                                                                                                                                      <  1:373581/245‑1 (MQ=255)
                                                                            gCCGTGGGTGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGc                                                                                                                                                                        >  1:344955/1‑251 (MQ=255)
                                                                               gTGGGTGCACGTTTTGATGAACGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑AAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTGACGACCATCCCGGTGACGCTAt                                                                                                                                                                     >  2:343191/1‑251 (MQ=255)
                                                                                        ggTTTTGATGACCGGGTGACCGGCAAACTGAACGCCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGGGGCATGACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑ATAGCAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                            <  1:269552/250‑1 (MQ=255)
                                                                                        cGTTTTGATGACCGGGTGACCGGCAAACTGANCACCTTCGCACCACACGCCCGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCGGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAAGGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                            <  2:227477/251‑1 (MQ=255)
                                                                                        cGTTTTGAGGACCGGGGGGCCGGCAAACGGACCACCTTCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGcc                                                                                                                                                            <  2:55947/251‑1 (MQ=255)
                                                                                         gTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCg                                                                                                                                                           >  2:133247/1‑251 (MQ=255)
                                                                                           ttttatgaCCGGGTGACCTGCAAACTGAATAACTTCGCCCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTGCCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGGCTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCgt                                                                                                                                                          <  1:44654/246‑1 (MQ=255)
                                                                                           tttGATGACCGGGTGACCGGCAAACTGAACGCCTTCGCCCCACACGCCAGGTTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCGCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgt                                                                                                                                                          <  2:147429/250‑1 (MQ=255)
                                                                                                       gTGACCGGCAAACTGAACACCTTCGCACCACACGCCAGTGTTAGCCATAGTGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAaaca                                                                                                                                             <  1:16133/251‑1 (MQ=255)
                                                                                                            cGGCAAACTGAACACCTTCGCACCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGAGCGCAGCTGCGTGATGAACATGCCTGGCGCTCCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTCAaacacatgt                                                                                                                                        >  2:135572/1‑246 (MQ=255)
                                                                                                             ggCAAACTGAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTc                                                                                                                                       >  2:438845/1‑251 (MQ=255)
                                                                                                                     gAACACCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGt                                                                                                                                <  2:353319/250‑1 (MQ=255)
                                                                                                                              cgcaccACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCgg                                                                                                                      <  1:165522/247‑1 (MQ=255)
                                                                                                                               gcaccACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCgg                                                                                                                      >  1:78025/4‑250 (MQ=255)
                                                                                                                                 accACACGCCAGTGTTATCCATATGGATATCCACCCGGCAGAAATGAACAAGCTGCGTCAGTCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCTTAAACCTGCGGAtt                                                                                                                   <  2:325911/250‑1 (MQ=255)
                                                                                                                                     cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGcgt                                                                                                               >  1:106543/1‑251 (MQ=255)
                                                                                                                                         ccAGTTTTCTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTCCCCGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAGCCTGCGGATTGCGTCGTg                                                                                                           <  2:383347/251‑1 (MQ=255)
                                                                                                                                          cAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTg                                                                                                           >  1:288500/1‑250 (MQ=255)
                                                                                                                                            gtgtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGAcc                                                                                                        >  1:237077/1‑251 (MQ=255)
                                                                                                                                                 aTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACcac                                                                                                      >  2:415599/1‑248 (MQ=255)
                                                                                                                                                         ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTgggg                                                                                           <  2:417501/250‑1 (MQ=255)
                                                                                                                                                                gACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAACACAACTGTCAGATCGTACACCTGCGGCTTGCGTTGTGACC‑CACGATGTGGAGCCGCAccc                                                                                   >  2:166260/1‑250 (MQ=255)
                                                                                                                                                                 aCCCTGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCAcc                                                                                    >  1:360699/1‑249 (MQ=255)
                                                                                                                                                                    cGGCAGAAATGCACAAGCTACGTCAGGAACCTGAGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAACCGTTAAATAT‑‑CAATGACTAGCAGCTACACTGCGAGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGAGCCGCTATCTACGCGACGTTGGTGTTAAAACAACTGTCAGATCGTAAACCTGCGTATTGCGTCGTGACCACA‑GATGTGGTGCAGGACCAGAt                                                                                >  2:164291/1‑250 (MQ=255)
                                                                                                                                                                    cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GCTGTGGGGCAGCACCAGAtg                                                                               >  1:214982/1‑251 (MQ=255)
                                                                                                                                                                       cAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTgg                                                                            <  2:428243/251‑1 (MQ=255)
                                                                                                                                                                           aaTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTg                                                                                                                         <  2:249282/203‑1 (MQ=255)
                                                                                                                                                                           aaTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTg                                                                                                                         >  1:249282/1‑203 (MQ=255)
                                                                                                                                                                             tGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGCTTTAACTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGCTCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGAAGCACAAGATGTGGGCTggg                                                                      >  2:185370/1‑249 (MQ=255)
                                                                                                                                                                                   aGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcac                                                                >  2:94589/1‑251 (MQ=255)
                                                                                                                                                                                       gCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCATCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCg                                                            <  1:43910/251‑1 (MQ=255)
                                                                                                                                                                                        cGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGc                                                           <  1:287213/251‑1 (MQ=255)
                                                                                                                                                                                         gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGAt                                                                                <  2:198611/229‑1 (MQ=255)
                                                                                                                                                                                         gTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGAt                                                                                >  1:198611/1‑229 (MQ=255)
                                                                                                                                                                                                            aCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACGCGCCCGGAAAAtt                                       >  2:73134/1‑251 (MQ=255)
                                                                                                                                                                                                             cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAAttt                                      >  1:128345/1‑251 (MQ=255)
                                                                                                                                                                                                                         aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAg                          <  1:232671/251‑1 (MQ=255)
                                                                                                                                                                                                                         aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAg                          <  1:151089/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCggct                     <  1:98811/248‑3 (MQ=255)
                                                                                                                                                                                                                                 gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTa                   <  2:377300/250‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAg                  <  1:227605/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTACCAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGGTACGACCATCCCGGGGACGCTAGCTACTCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCATCACCATATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcat                                   <  1:383082/234‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTACCAGCATTACAGCACCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGAGCAGCTGCGTGATGAACATGCCTGGCGTTAAGACCATCCCGGTGACGCTATATACGCGACGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCCGATGTGGGCCGCGCAGCACATCGCACACACTCGCCCGGCAAACTtcatagataggaaccgcgtcg                  >  2:383082/1‑234 (MQ=255)
                                                                                                                                                                                                                                   tcccAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGCTCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCggcctcggc                >  2:9689/3‑244 (MQ=255)
                                                                                                                                                                                                                                     ccAGCATTACAGCAGCCGTTAAATAT‑‑CAATGACTGGCAGCTACACTTCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggcac              <  1:398817/251‑4 (MQ=255)
                                                                                                                                                                                                                                             aCAGCAGCCGTTAAATAT‑‑CACTGACGGGCAGCTACACTGCGCGCCGCTCCGTGATGAACATGCCAGACGATACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGATAAAACAACTGTCAGATCGTAAACCTGCGGTTTGCGTCGTGACCACA‑GATGTGGCCCAGCCCCAGAAGTGGACTGGGCAGCACATCGCCCACACTCGCCAGGAAAATTTCATCACCTCCAGCGCCTTAGGCACCCTgggct      >  2:380851/1‑249 (MQ=255)
                                                                                                                                                                                                                                                 cagcCGTTAAATAT‑‑CAATGACTGGCCGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCAAA‑GATGTGGGGCAGCACCAGCTGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGATTTgg  >  2:330203/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 cagcCGTTAAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTgg  <  1:264458/251‑1 (MQ=255)
                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                  
GTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACA‑GATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGG  >  NC_000913/3951285‑3951774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑