Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,508,293	A→G	intergenic (‑51/+168)	fryB ← / ← glk	putative PTS enzyme IIB component FryB/glucokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,508,293	0	A	G	100.0%	146.5 / NA	38	intergenic (‑51/+168)	fryB/glk	putative PTS enzyme IIB component FryB/glucokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/16);  total (22/16)

GGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGAT  >  NC_000913/2508051‑2508537                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ttaattacccttgcgcccatattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAAt                                                                                                                                                                                                                                               <  2:344903‑M2/58‑1 (MQ=255)    attacccttgcgcccatattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGcc                                                                                                                                                                                                                                           <  2:35542‑M2/62‑1 (MQ=255)                  catattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccgtgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat                                                                                                                                                                                                                             <  2:262636‑M2/76‑1 (MQ=255)                     attacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat                                                                                                                                                                                                                          >  1:130148‑M2/173‑251 (MQ=255)                               agttgagttccgaaaggtccattaaggacatgtgtcatatcgataactagtaagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg                                                                                                                                                                                                                >  1:276597‑M2/163‑251 (MQ=255)                               agttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGttaccagacaaataacccattgatg                                                                                                                                                                                                                 >  1:225107‑M2/163‑240 (MQ=255)                                           aaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc                                                                                                                                                                                                    >  1:495897‑M2/151‑251 (MQ=255)                                                      aaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg                                                                                                                                                                                          >  1:427482‑M2/140‑250 (MQ=255)                                                             tgtgtcatatcgataactaataagccttcaaacggcactttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGtgtg                                                                                                                                                                                   >  1:362264‑M2/133‑250 (MQ=255)                                                                          taactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGCAGTgtgacgcagtgcgccct                                                                                                                                                                     >  2:189158‑M2/120‑243 (MQ=255)                                                                                  aagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACTCAGGTCGACCTTGTGAgg                                                                                                                                                              <  2:407729‑M2/139‑1 (MQ=255)                                                                                           aacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACaa                                                                                                                                                      >  1:188038‑M2/103‑249 (MQ=255)                                                                                             cggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaa                                                                                                                                                  <  2:304525‑M2/151‑1 (MQ=255)                                                                                                         ttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAt                                                                                                                                                                                                      <  1:264929‑M2/99‑1 (MQ=255)                                                                                                         ttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAt                                                                                                                                                                                                      >  2:264929‑M2/89‑187 (MQ=255)                                                                                                              tatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGt                                                                                                                                  <  1:250707‑M2/167‑1 (MQ=255)                                                                                                                   gtcatttgcttatgccttgaaaaaaaccgaaggtgaaaagaaacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGAt                                                                                                                             <  2:79940‑M2/172‑1 (MQ=255)                                                                                                                          gcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAg                                                                                                                      <  1:350307‑M2/179‑1 (MQ=255)                                                                                                                                           agccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg                                                                                                                                                                                       <  1:344767‑M2/114‑1 (MQ=255)                                                                                                                                           agccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg                                                                                                                                                                                       >  2:344767‑M2/55‑168 (MQ=255)                                                                                                                                               gaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTccc                                                                                                >  1:487132‑M2/51‑251 (MQ=255)                                                                                                                                                    tgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGagttaccccccctggg                                                                                           >  2:132026‑M2/46‑246 (MQ=255)                                                                                                                                                            cacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGAGAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACAGTATGATTTAAAAGATTATCGGGAGAGTTCCCTCCCGATATAAaaggaa                                                                                   <  2:438156‑M2/214‑1 (MQ=255)                                                                                                                                                                      tttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAACAGATTATCGGGAGCGTTACCTCCCGATATAAAAGGAAGTATTTAc                                                                           >  2:125709‑M2/28‑249 (MQ=255)                                                                                                                                                                           gtaaaatcaataacatgattaaCGTTAATTCNTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACGGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAAtgt                                                                    <  2:217837‑M2/229‑1 (MQ=255)                                                                                                                                                                               aatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGAcc                                                                <  2:141025‑M2/233‑1 (MQ=255)                                                                                                                                                                                   aataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGGGCAGAACAAATCGCCGGTACCAGACAAATAATGCAGTTACGGGAGAATTAATCACGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGAAACAACGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGCTTTACAGAATGTGACCTa                                                              >  1:7381‑M2/15‑249 (MQ=255)                                                                                                                                                                                           gattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCg                                                    >  2:116942‑M2/7‑251 (MQ=255)                                                                                                                                                                                                  gatAATTCCTCGTACAGTGATAGTCGACGAGTGAAGTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTATTGGAGAATTAATCGCGCTATACGGCAGAGAGACGCAGGTCGACCTTGCTAGTGGACACAAAAAAAAAATGAATAAACTGTAAGATTTACAAGATTATCGGGAGAGTTACCTCCAGATATACAAGGAAGGATTAACAGAATCAGACCTAAGGTCTGGCGTaagagg                                             >  2:271813/3‑247 (MQ=255)                                                                                                                                                                                                        tCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCg                                       >  1:52967/1‑251 (MQ=255)                                                                                                                                                                                                             gTTCAGTGATAGTCGACTAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTCCCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCgtaccc                                  <  2:385301/251‑6 (MQ=255)                                                                                                                                                                                                                 aGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCg                                 >  2:349764/1‑248 (MQ=255)                                                                                                                                                                                                                        gtcgACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCg                       <  2:99584/248‑1 (MQ=255)                                                                                                                                                                                                                               aGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTc                >  1:413859/1‑251 (MQ=255)                                                                                                                                                                                                                                         tGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAttt                                                                             >  1:307264/1‑180 (MQ=255)                                                                                                                                                                                                                                         tGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCGGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAttt                                                                             <  2:307264/180‑1 (MQ=255)                                                                                                                                                                                                                                          gAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATg      >  2:112735/1‑250 (MQ=255)                                                                                                                                                                                                                                             cGTGCAGAACAACTCGCCGTTACCAGACCAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGCAGTGTGACGCAGGTCGACCTTGTGAGGGGCCACAAAGCAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCGCGATATCAAAGGAAGGATTTACAGACAGTGACCTAAGGTCTGCCGTAAATGTGCCCCGGAATCNAGAAGGCCCGGATTGCCATGGaagcttggat  >  2:218715/1‑242 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     GGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGAT  >  NC_000913/2508051‑2508537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑