Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,508,268	T→C	intergenic (‑26/+193)	fryB ← / ← glk	putative PTS enzyme IIB component FryB/glucokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,508,268	0	T	C	100.0%	121.1 / NA	33	intergenic (‑26/+193)	fryB/glk	putative PTS enzyme IIB component FryB/glucokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/15);  total (18/15)

TAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCG  >  NC_000913/2508041‑2508506                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               cggctcaactttaattacccttgcgcccatattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGc                                                                                                                                                                                                                         <  1:81669‑M2/49‑1 (MQ=255)           ttaattacccttgcgcccatattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAAt                                                                                                                                                                                                                <  2:344903‑M2/58‑1 (MQ=255)              attacccttgcgcccatattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGcc                                                                                                                                                                                                            <  2:35542‑M2/62‑1 (MQ=255)                            catattacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccgtgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat                                                                                                                                                                                              <  2:262636‑M2/76‑1 (MQ=255)                               attacaaagaagttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat                                                                                                                                                                                           >  1:130148‑M2/173‑251 (MQ=255)                                         agttgagttccgaaaggtccattaaggacatgtgtcatatcgataactagtaagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTg                                                                                                                                                                                 >  1:276597‑M2/163‑251 (MQ=255)                                         agttgagttccgaaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGttaccagacaaataacccattgatg                                                                                                                                                                                  >  1:225107‑M2/163‑240 (MQ=255)                                                     aaaggtccattaaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATcc                                                                                                                                                                     >  1:495897‑M2/151‑251 (MQ=255)                                                                aaggacatgtgtcatatcgataactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACgg                                                                                                                                                           >  1:427482‑M2/140‑250 (MQ=255)                                                                       tgtgtcatatcgataactaataagccttcaaacggcactttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGtgtg                                                                                                                                                    >  1:362264‑M2/133‑250 (MQ=255)                                                                                    taactaataagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGCAGTgtgacgcagtgcgccct                                                                                                                                      >  2:189158‑M2/120‑243 (MQ=255)                                                                                            aagccttcaaacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACTCAGGTCGACCTTGTGAgg                                                                                                                               <  2:407729‑M2/139‑1 (MQ=255)                                                                                                     aacggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACaa                                                                                                                       >  1:188038‑M2/103‑249 (MQ=255)                                                                                                       cggccctttgctttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaa                                                                                                                   <  2:304525‑M2/151‑1 (MQ=255)                                                                                                                   ttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAt                                                                                                                                                                       <  1:264929‑M2/99‑1 (MQ=255)                                                                                                                   ttcattatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAt                                                                                                                                                                       >  2:264929‑M2/89‑187 (MQ=255)                                                                                                                        tatttgtcatttgcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGt                                                                                                   <  1:250707‑M2/167‑1 (MQ=255)                                                                                                                             gtcatttgcttatgccttgaaaaaaaccgaaggtgaaaagaaacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGAt                                                                                              <  2:79940‑M2/172‑1 (MQ=255)                                                                                                                                    gcttatgccttgaaaaaagccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAg                                                                                       <  1:350307‑M2/179‑1 (MQ=255)                                                                                                                                                     agccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg                                                                                                                                                        >  2:344767‑M2/55‑168 (MQ=255)                                                                                                                                                     agccgaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAg                                                                                                                                                        <  1:344767‑M2/114‑1 (MQ=255)                                                                                                                                                         gaaggtgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTccc                                                                 >  1:487132‑M2/51‑251 (MQ=255)                                                                                                                                                              tgaaaagacacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGagttaccccccctggg                                                            >  2:132026‑M2/46‑246 (MQ=255)                                                                                                                                                                      cacttcggcatttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGAGAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACAGTATGATTTAAAAGATTATCGGGAGAGTTCCCTCCCGATATAAaaggaa                                                    <  2:438156‑M2/214‑1 (MQ=255)                                                                                                                                                                                tttatgtaaaatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAACAGATTATCGGGAGCGTTACCTCCCGATATAAAAGGAAGTATTTAc                                            >  2:125709‑M2/28‑249 (MQ=255)                                                                                                                                                                                     gtaaaatcaataacatgattaaCGTTAATTCNTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACGGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAAtgt                                     <  2:217837‑M2/229‑1 (MQ=255)                                                                                                                                                                                         aatcaataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGAcc                                 <  2:141025‑M2/233‑1 (MQ=255)                                                                                                                                                                                             aataacatgattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGGGCAGAACAAATCGCCGGTACCAGACAAATAATGCAGTTACGGGAGAATTAATCACGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGAAACAACGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGCTTTACAGAATGTGACCTa                               >  1:7381‑M2/15‑249 (MQ=255)                                                                                                                                                                                                     gattaaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCg                     >  2:116942‑M2/7‑251 (MQ=255)                                                                                                                                                                                                            gatAATTCCTCGTACAGTGATAGTCGACGAGTGAAGTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTATTGGAGAATTAATCGCGCTATACGGCAGAGAGACGCAGGTCGACCTTGCTAGTGGACACAAAAAAAAAATGAATAAACTGTAAGATTTACAAGATTATCGGGAGAGTTACCTCCAGATATACAAGGAAGGATTAACAGAATCAGACCTAAGGTCTGGCGTaagagg              >  2:271813/3‑247 (MQ=255)                                                                                                                                                                                                                  tCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCg        >  1:52967/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTCAGTGATAGTCGACTAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTCCCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCgtaccc   <  2:385301/251‑6 (MQ=255)                                                                                                                                                                                                                           aGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCg  >  2:349764/1‑248 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               TAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCG  >  NC_000913/2508041‑2508506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑