Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,476,629	A→G	intergenic (+119/+65)	torI → / ← dsdC	prophage CPS‑53 recombination directionality factor and response regulator inhibitor/DNA‑binding transcriptional dual regulator DsdC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,476,629	0	A	G	100.0%	176.6 / NA	49	intergenic (+119/+65)	torI/dsdC	prophage CPS‑53 recombination directionality factor and response regulator inhibitor/DNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/20);  total (29/20)

ACCTTCATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATC  >  NC_000913/2476382‑2476874                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aCCTTCATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCATAAAACCACATAAAGAcc                                                                                                                                                                                                                                                    >  1:181224/1‑251 (MQ=255)      cATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaa                                                                                                                                                                                                                                                >  2:32036/1‑250 (MQ=255)         cTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaatt                                                                                                                                                                                                                                             >  1:439525/1‑250 (MQ=255)           aTGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGtaaattaa                                                                                                                                                                                                                                           >  1:107447/1‑250 (MQ=255)                     tAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCAAGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCCCATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTCGATGTCTGCAGGGGACACCATCCTTATGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTac                                                                                                                                                                                                                                >  2:180708/1‑250 (MQ=255)                      aaGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGc                                                                                                                                                                                                                               <  1:52236/251‑1 (MQ=255)                                        aaaGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCAATGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACAACATCAAGCCCGTAAATTNAATTCGAACTAGCAGGCCGAATAAGGCccac                                                                                                                                                                                                             >  2:254404/1‑251 (MQ=255)                                          ggCCAAAGTTATGCACGGGCGAGCAAGATGGTTATCTCGTGACCATTGTGAATTAAAAAATAAGCTATTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAACACACCATTCCAATCAATACCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGCTTATCCCTAAAACCACATAAAGACCGTAAATTAATTTCAATCTAGCATGCCGACTAAGGCCCAcc                                                                                                                                                                                                            >  2:402203/2‑250 (MQ=255)                                                 gTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTc                                                                                                                                                                                                    <  1:174021/251‑1 (MQ=255)                                                   tATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCGGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCg                                                                                                                                                                                                   <  2:476606/250‑1 (MQ=255)                                                           ggCGAGCAAGATGGTTATCTCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAt                                                                                                                                                                                          >  2:417300/1‑251 (MQ=255)                                                            gCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAtt                                                                                                                                                                                         <  2:451340/251‑1 (MQ=255)                                                                  aaGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTAt                                                                                                                                                                                   >  1:293123/1‑251 (MQ=255)                                                                       ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAg                                                                                                                                                                               <  1:429583/250‑1 (MQ=255)                                                                                     cATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGtt                                                                                                                                                                <  1:306946/251‑1 (MQ=255)                                                                                      aTTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTATCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtc                                                                                                                                                               <  1:325629/251‑1 (MQ=255)                                                                                         gtgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGAAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTctct                                                                                                                                                            >  1:466638/1‑251 (MQ=255)                                                                                           gAATTCAACAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGAGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCtt                                                                                                                                                           >  2:477418/1‑250 (MQ=255)                                                                                            aaTTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAg                                                                                                                                                         <  2:75671/251‑1 (MQ=255)                                                                                             aTTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGc                                                                                                                                                        >  1:207531/1‑251 (MQ=255)                                                                                                 aaaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                                                                                                                                                       >  2:446172/1‑248 (MQ=255)                                                                                                     aTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataat                                                                                                                                                >  1:389778/1‑251 (MQ=255)                                                                                                            ctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACAATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACACGGAAATCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCt                                                                                                                                         >  2:422856/1‑251 (MQ=255)                                                                                                               aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTc                                                                                                                                       >  2:91664/1‑250 (MQ=255)                                                                                                                       ccAATGGGTAAAATAGCGGGTAAAATATTTCTCACAGCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCATGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTgg                                                                                                                              <  2:431773/251‑1 (MQ=255)                                                                                                                            gggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCAct                                                                                                                         >  1:282617/1‑251 (MQ=255)                                                                                                                                     aGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATTCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCg                                                                                                                >  1:127189/1‑251 (MQ=255)                                                                                                                                     aGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCg                                                                                                                <  1:446172/251‑1 (MQ=255)                                                                                                                                                              aaaaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATACGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTg                                                                                       >  2:36931/1‑251 (MQ=255)                                                                                                                                                              aaaaaaCACCAATCCAATCAATCCCCTGCTGTTTCTAGTAGATGTCTGCAGGGGACACCATACTTTTGTTTATCCATAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCATACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCTGGTAATGTGGTGATGtaatact                                                                                           <  2:19079/247‑3 (MQ=255)                                                                                                                                                                aaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGAt                                                                                     >  1:63190/1‑251 (MQ=255)                                                                                                                                                                           ccAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATTTAATAATGCTGATGGCATTTCAc                                                                           <  1:140416/250‑1 (MQ=255)                                                                                                                                                                           ccAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGc                                                                                  <  1:354929/243‑1 (MQ=255)                                                                                                                                                                           ccAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGc                                                                                  >  2:354929/1‑243 (MQ=255)                                                                                                                                                                              atcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCa                                                                                                                                                      >  2:488186/1‑172 (MQ=255)                                                                                                                                                                              atcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCa                                                                                                                                                      <  1:488186/172‑1 (MQ=255)                                                                                                                                                                                            gctTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACgg                                                         <  2:107447/251‑1 (MQ=255)                                                                                                                                                                                               tCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGgcg                                                       <  1:452457/250‑1 (MQ=255)                                                                                                                                                                                                      gATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGc                                               >  1:99607/1‑251 (MQ=255)                                                                                                                                                                                                            tGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTcacc                                          >  2:262305/1‑250 (MQ=255)                                                                                                                                                                                                             gCAGGGGACACCATCCTTGTGTTGAGCCCTAAAACCACATAAAGAGCTAAAATTAAATTCGAACTAGCAGGCCGAATAAGTCACATCAGGATCTTGTTGTTTTATGCATTAGGAAGTGGTTTTTACCTGTGCTTTTAGCCAGATAATAAACGCTGCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCAAATCGCCAAACGGCGCGACGAGCTcacc                                          <  2:286697/249‑1 (MQ=255)                                                                                                                                                                                                               aGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTg                                      >  1:488175/1‑251 (MQ=255)                                                                                                                                                                                                               aGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGATTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCANATCGCCAAACGGCGCGACGAGCTcaccattg                                      >  2:242022/1‑248 (MQ=255)                                                                                                                                                                                                                     cacCATCCTTGTGTTTATCCCTAAAACCACATAAAGACCGTAAAGTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCTTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTTATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGc                                <  2:447835/251‑1 (MQ=255)                                                                                                                                                                                                                                    tATCCCTAAAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAg                  <  1:118872/250‑1 (MQ=255)                                                                                                                                                                                                                                       cccTACAACCACATAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGt              >  2:146342/1‑251 (MQ=255)                                                                                                                                                                                                                                          aaaaaCCACATAAAGACCGAAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGtttt           <  2:293123/250‑1 (MQ=255)                                                                                                                                                                                                                                                   aTAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATc  >  1:233370/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aTAAAGACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATc  >  1:128985/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ACCTTCATCTATGACCGGATTAAGTCAGGCGACCTGCCAAAAGCCAAAGTTATCCACGGGCGAGCAAGATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTCAATTTTTGGCCACTGCCTGCCCGGTAATGTGGTGATGTAATAATGCTGATGGCATTTCACCGTCATATCGCCAAACGGCGCGACGAGCTCACCACTGGCAAGCCTTTTTTGTACCAGGCGTTTTCTTCCCATC  >  NC_000913/2476382‑2476874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑