Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,093,977	C→T	H170H (CAC→CAT)	hisB →	imidazoleglycerol‑phosphate dehydratase/histidinol‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,093,977	0	C	T	100.0%	192.8 / NA	53	H170H (CAC→CAT)	hisB	imidazoleglycerol‑phosphate dehydratase/histidinol‑phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/33);  total (20/33)

TTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC  >  NC_000913/2093743‑2094218                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  ttGTCCGCACCGCCCCGCCGATGAGTGCGACGGCCGGAAGCCGAAATTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGTCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATAcc                                                                                                                                                                                                                                    <  2:211319/250‑1 (MQ=255)  tGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCa                                                                                                                                                                                                                                   >  2:70029/1‑250 (MQ=255)     cggCACCGGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga                                                                                                                                                                                                                               <  1:201151/249‑1 (MQ=255)           cTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGc                                                                                                                                                                                                                          >  1:287317/1‑250 (MQ=255)             gCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGc                                                                                                                                                                                                                          >  1:398371/1‑248 (MQ=255)                     atgattgggACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATAGCGCTAACAGTTATGTGATTGGCGCTCGCGCGACCGACATTCAACTGGCGGAAACCATGGGCATTACTGGTTTCCGCCACGACCGCGCACACCTTAACTGGCCAATGATTGGCGAGCAACTCACCCGACGTGACCGGTACGCTCATGTAGTGCGTAATTCCAAagcaatgccgcttcacctt                                                                                                                                                                                                               >  2:133170/9‑234 (MQ=255)                            cGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCGGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATGGGCGATCGCGCGACCGACATGCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACGCACCAGACGTGTCCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt                                                                                                                                                                                                        <  2:489316/251‑1 (MQ=255)                                 gCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt                                                                                                                                                                                                    <  2:145496/250‑1 (MQ=255)                                 gCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGGGTGGCTg                                                                                                                                                                                                   >  2:374596/1‑251 (MQ=255)                                 gCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCGGGCTGAGCAAGCGATGTATCGCGCTAACAGTTATGTGATTGGCGATCGCTCGACCGACATTCAACTGGCGGAAAACATGGGCATTTCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGTCGTTCAGGTGTGGCt                                                                                                                                                                                                    <  1:366583/250‑1 (MQ=255)                                 gCCGTAAGCCGAAAGAAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAAGTGTGGCTg                                                                                                                                                                                                   >  1:328619/1‑251 (MQ=255)                                       aGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGGGGGTAATACCAAAGAGACGCAGATTGACGATCAGGTGTGGCTGGAtagg                                                                                                                                                                                             >  2:32773/1‑248 (MQ=255)                                                   aaCTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATAGTGACGGGGgcagc                                                                                                                                                                                 >  2:401358/1‑251 (MQ=255)                                                            aaCGTTATCTGGCTGAGCAAGCGATGGCTCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATAGGCATTACTGGTTTACGCTACGACCGCGAAGCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATTCCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAac                                                                                                                                                                        <  1:152354/251‑1 (MQ=255)                                                                   tCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGGTTGGCGCTCGCGCGACCGACATTCAACTGGCGGAAAACATGAGCATTACTGGTTTCCGCTACGACCGCGACACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCCGATCGACGTTCCGGTGTGGCTGGATCGTGAAGGTGgcagcataattaccaacggcg                                                                                                                                                                 >  2:178590/1‑236 (MQ=255)                                                                           aGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGcttc                                                                                                                                                         <  1:297356/251‑1 (MQ=255)                                                                            gCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGcttct                                                                                                                                                        <  1:478801/251‑1 (MQ=255)                                                                                        aagcgcTAACAGTTATGTGATTGGGGATGGCNCGTCCGACATTCAACTGGTGGAAAAAAGTGGAATTACTGGCGTACTCGACGACCGCGAAACCCTGAACTGGCCAATGATTGTCGAGCAACTCACCAGACGTGACCGTTACGCTTATTTAGTGCGTAATATCAAAGAGACGCAGATTGACTTTCAGGTGTGGCTGGATCGTGAAGTTGGCAGCACGATTAACCCCGGCGTTTGCTTCTTTTATCAGATGc                                                                                                                                            <  2:222656/249‑1 (MQ=255)                                                                                            gcTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTgg                                                                                                                                         >  2:80539/1‑250 (MQ=255)                                                                                                aCAGTTATGTGATTGGCGATCGCGCGACCGACAGTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAg                                                                                                                                    <  2:290287/251‑1 (MQ=255)                                                                                                  aGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGAt                                                                                                                                  <  1:70029/251‑1 (MQ=255)                                                                                                   gTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGAtt                                                                                                                                 <  1:184913/251‑2 (MQ=255)                                                                                                      aTGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGGGCGTAATACCAAAGAGACGCAGATTGACGTGCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCt                                                                                                                              <  2:187704/251‑1 (MQ=255)                                                                                                                  aTCGCGCGACCGACATTCAACTGGCGGAAACCAGGGGCATTACTGGTTTACGATACGACCGCGAAACCCTGAACTGGCCAATGATGGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTGCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGAACGCGACCCAcggcggg                                                                                                                  <  2:277486/251‑2 (MQ=255)                                                                                                                       gcgACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTccc                                                                                                             >  1:227256/1‑250 (MQ=255)                                                                                                                         gaccgacATTAAACTGGCGGAAAACATGGGCATTAATGGTTTACGCTACGAACGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTAAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGATTCTTTGCTCATATTCTGGACCAGATCGCGACCCACGGCGGTTTACGc                                                                                                            >  1:376251/1‑250 (MQ=255)                                                                                                                           ccgacctgcAACTGGCGGAAAACAGGGGCATTACTGGTCTACGATACGACCGCTAAACCCGGAACTGGGCAATGATTGGCGAGCAACTCACCAGACGTGGCCGTTACGCTCATGTAGAGCGTAATGCCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACCCCTGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCGCGGAGGTTTCCGCATg                                                                                                         <  2:361638/243‑1 (MQ=255)                                                                                                                                    aaCTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg                                                                                                                                                                                     <  2:139954/166‑1 (MQ=255)                                                                                                                                    aaCTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg                                                                                                                                                                                     >  1:139954/1‑166 (MQ=255)                                                                                                                                     aCTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACGCCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACg                                                                                               <  2:287317/251‑1 (MQ=255)                                                                                                                                        ggCGGAATACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCa                                                                                            <  1:473565/251‑1 (MQ=255)                                                                                                                                        ggCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGt                                                                                              <  1:80539/249‑1 (MQ=255)                                                                                                                                         gCGGAAAACCTGGGCATTACTGGCTTACGCTACGCCCGCCAACCCCTGAACTGGCCAATGAATGGCGAGCAACCCACCAGACGTGACCGTTACGCTCATGTAGTGCGGAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTTGATCAGATCGCGTCCCGCGGCGGTTTCCGCATGGAAATCAACGtaaa                                                                                           <  2:106383/251‑4 (MQ=255)                                                                                                                                            gAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCCAAgg                                                                                        >  1:262185/1‑251 (MQ=255)                                                                                                                                             aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGc                                                                                       <  2:262185/251‑1 (MQ=255)                                                                                                                                                      gCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCtat                                                                              <  1:417518/251‑1 (MQ=255)                                                                                                                                                          tACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATc                                                                           <  1:496857/250‑1 (MQ=255)                                                                                                                                                          tACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGAGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCCAAAGCGCACTCTATATcg                                                                          >  1:335544/1‑251 (MQ=255)                                                                                                                                                              ggtgtccGCTACGACCGCGAGACCCCGAACAGGACAATGATTTGCGGGCAACTCACCAGACGTGACCGTTACGATCATGTAGTGCGTAATCCCAAAGAGACGCAGATTGGCGTTCAGGTGTTGCTGGATCGTGAAGGGGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGCCCTCTATAGcgac                                                                        <  2:15701/243‑1 (MQ=255)                                                                                                                                                              ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGAGCGTGAAGGTGGCAGCAAGATTAACTCCGTCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATcgacga                                                                      <  1:448800/251‑1 (MQ=255)                                                                                                                                                                       tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCacac                                                             >  1:493149/1‑251 (MQ=255)                                                                                                                                                                        aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACcaca                                                              <  1:401358/249‑1 (MQ=255)                                                                                                                                                                             cgcgAAACCCTGAACTGGGCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACAcc                                                                                                                                                                     >  2:418902/1‑141 (MQ=255)                                                                                                                                                                             cgcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACAcc                                                                                                                                                                     <  1:418902/141‑1 (MQ=255)                                                                                                                                                                               cgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCCCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAg                                                     <  2:356779/251‑1 (MQ=255)                                                                                                                                                                                             ggCCAATGATTGGCGAGCAACCCACCAGACGTGACCTTTACGCTCATGTAGTGCGTTATACCAAAGAGACGCAGATTGACGTGCAGGTGTGGCTGGGTCGTGAAGGTGGCAGCAAGAATAACACCGGCGTTGGCTTCGTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCGCTATATCGACGATCACCACACCGCCGAAGATACCGGCCTGgc                                        <  2:419390/250‑1 (MQ=255)                                                                                                                                                                                                  aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTggg                                  <  2:257828/251‑1 (MQ=255)                                                                                                                                                                                                    gATTGGCGAGCAAGTCACCAGACGTGACCGTTAAGCTCATGTAGTGCGGAAGACCAAAGAGACGTAGATTGACGTTCAGGTGTGGGTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATAGCGACGATCACGACTCCGTCGAAGATACCGGCCTGGCGCTGGGc                                 <  1:409153/250‑1 (MQ=255)                                                                                                                                                                                                     aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGa                               <  1:160281/251‑1 (MQ=255)                                                                                                                                                                                                                   cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGGCAAAGGCGACCTCTATATCGACGATCACCCCACCGTCGAAGATAACGGCCCGGCGCTGGGCGCAGCGCTAAAAATcc                 >  1:367531/1‑250 (MQ=255)                                                                                                                                                                                                                               ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGGTGGCTTCTTTGCTCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCAAGGGAATCAACGACAAAGGCGCCCTCTATATCGCCGATCACCACACCGGCGACAATACCGGCCTCGCGCCGGGGGCACCGCTAAAAATCGCCCTCGGAGAc      >  2:9174/1‑250 (MQ=255)                                                                                                                                                                                                                                  ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc  >  1:104557/1‑251 (MQ=255)                                                                                                                                                                                                                                   tACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATGGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc  <  1:33912/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                  TTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAAAGTAAAACTGGTGGAACGTTATCTGGCTGAGCAAGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC  >  NC_000913/2093743‑2094218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑