Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,089,621	A→G	R31R (CGT→CGC)	yefM ←	YefM antitoxin of the YoeB‑YefM toxin‑antitoxin pair and DNA binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,621	0	A	G	100.0%	188.3 / NA	51	R31R (CGT→CGC)	yefM	YefM antitoxin of the YoeB‑YefM toxin‑antitoxin pair and DNA binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/21);  total (30/21)

TGATCTTTTTAACAATTCGCTTATCTGTTTCCTGCCAGTACAGATAATCGTCCCATGATTCCTCAGACCAGATTAGTTTCACTCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCC  >  NC_000913/2089384‑2089867                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       ccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGCGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg                                                                                                                                                                                                                                            >  2:264719‑M2/88‑250 (MQ=255) ccgttatcgcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg                                                                                                                                                                                                                                            <  1:364507‑M2/163‑1 (MQ=255)          cctgatacaccttagatcaataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCggg                                                                                                                                                                                                                                      <  2:16567‑M2/169‑1 (MQ=255)                acaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCtt                                                                                                                                                                                                                            >  1:198655‑M2/73‑251 (MQ=255)                 caccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc                                                                                                                                                                                                                           >  2:15001‑M2/72‑251 (MQ=255)                  accttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc                                                                                                                                                                                                                           >  1:137897‑M2/71‑250 (MQ=255)                   ccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCa                                                                                                                                                                                                                          >  2:146690‑M2/70‑250 (MQ=255)                      tagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAAc                                                                                                                                                                                                                        <  1:146690‑M2/183‑1 (MQ=255)                           gtataaggctacgctagcgtatcaaaactgacaaatcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTATTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGGAANAAGGAACGGGGCATGCTCTTTAACGGCTTtc                                                                                                                                                                                                                 >  2:238552‑M2/62‑251 (MQ=255)                             ataagtcaacgtaagcgtatcaaaactgaaaattcattctatgatgtaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGGTTTGGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGCCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCttt                                                                                                                                                                                                                  <  2:324159‑M2/189‑1 (MQ=255)                             ataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat                                                                                                                                                                                                               >  2:460738‑M2/60‑251 (MQ=255)                                  gctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg                                                                                                                                                                                                          >  2:3217‑M2/55‑251 (MQ=255)                                          agcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGAc                                                                                                                                                                                                  <  1:56805‑M2/205‑1 (MQ=255)                                                                       gaatgaatctgttcaatAACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGGTATCGATTGAGTCCATCAATCGCCGGGCGGTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAt                                                                                                                                                                     <  1:491678‑M2/234‑1 (MQ=255)                                                                                  ttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGt                                                                                                                                                          >  1:456227‑M2/7‑251 (MQ=255)                                                                                    cAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTc                                                                                                                                                         >  1:396167/1‑250 (MQ=255)                                                                                                tttCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCGACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCtttt                                                                                                                                            >  2:364703/1‑251 (MQ=255)                                                                                                       tCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAg                                                                                                                                      <  2:175887/250‑1 (MQ=255)                                                                                                       tCCTTTGCCTGATTGCAGGCTACCGATTGAGGCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCAATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTAACCATTGTTGCCGACAAATTCTGACGCGTTTCGCTGGAGCTAATNGTAGGCATGCCAATCCCCTCTTGTGTACAGt                                                                                                                                     >  2:218673/1‑251 (MQ=255)                                                                                                        ccTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCATCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTAGCTGTAGCTAATTGTACGCATGTCAATCTACTCATTTGTACAGtt                                                                                                                                    >  2:456835/1‑251 (MQ=255)                                                                                                           ttGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCAt                                                                                                                                 >  1:37026/1‑251 (MQ=255)                                                                                                              ccTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGt                                                                                                                              >  1:422587/1‑251 (MQ=255)                                                                                                                tGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTAc                                                                                                                            >  1:223159/1‑251 (MQ=255)                                                                                                                 gttttCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCGTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTAc                                                                                                                            <  2:396167/248‑1 (MQ=255)                                                                                                                 gATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTAc                                                                                                                            <  1:349847/250‑1 (MQ=255)                                                                                                                     tCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAatg                                                                                                                        >  2:195137/1‑250 (MQ=255)                                                                                                                          cTATCGATTGAGTCCATCACTCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg                                                                                                                                                                                                                                                    >  1:480224/1‑121 (MQ=255)                                                                                                                           tATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATACGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCACCTCTTTTGTACAGTTCATTGTAAACTGATGAGCg                                                                                                                 >  1:247249/1‑251 (MQ=255)                                                                                                                                aTTGAGTCCATCAATCTCCGGGCGTTGGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGATTGCCATCAGAACACAATCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGttaat                                                                                                            <  1:456330/251‑1 (MQ=255)                                                                                                                                   gAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGttaatta                                                                                                          <  1:195137/250‑1 (MQ=255)                                                                                                                                    aGTCAATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGGGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAAc                                                                                                        <  2:198655/251‑1 (MQ=255)                                                                                                                                        cATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACtatt                                                                                                    >  2:43918/1‑251 (MQ=255)                                                                                                                                           aattttcCGGGCGTTAGGGGGGGAGCGCAGTATATAACCCGTCTCTTCCAGCGATGTGTTTGCTTCGAGGTACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGTGCGTTAATTAACtattta                                                                                                  <  2:413539/245‑1 (MQ=255)                                                                                                                                                 ccgagCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTattaatt                                                                                            >  2:339631/5‑250 (MQ=255)                                                                                                                                                   gggCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTc                                                                                                                                                                                            >  2:380585/1‑152 (MQ=255)                                                                                                                                                   gggCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTc                                                                                                                                                                                            <  1:380585/152‑1 (MQ=255)                                                                                                                                                   gggCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGt                                                                                         <  1:460738/251‑1 (MQ=255)                                                                                                                                                       gttgtcGGGGGACGGCAGTAAATAAGCTGTCTCTTCCAGCGATTTGAATTCTTCGAGGAACGTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTGTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGt                                                                                     <  2:102689/246‑1 (MQ=255)                                                                                                                                                                        gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                                                                                                                                                                             >  2:242053/1‑114 (MQ=255)                                                                                                                                                                        gTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca                                                                                                                                                                                                             <  1:242053/114‑1 (MQ=255)                                                                                                                                                                             actggcggcgggcattccGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTga                                                                <  1:401639/233‑1 (MQ=255)                                                                                                                                                                                   gTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGaa                                                          >  2:205962/1‑250 (MQ=255)                                                                                                                                                                                     cctttCCATCGATTTGTATTCTTCTAGTGACTGCAGAACACAAGCCTCGCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTAGTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAAt                                                       <  2:48860/248‑1 (MQ=255)                                                                                                                                                                                             gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCtt                                               >  2:264612/1‑251 (MQ=255)                                                                                                                                                                                              cGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTTGCTGGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTGCAGTTCATTGTACAATGATGAGCGTTAATTAGCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTc                                              <  1:282158/251‑1 (MQ=255)                                                                                                                                                                                               gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCg                                             >  2:493794/1‑251 (MQ=255)                                                                                                                                                                                                            cGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGc                                >  1:48546/1‑251 (MQ=255)                                                                                                                                                                                                                  aCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATc                            >  2:55200/1‑249 (MQ=255)                                                                                                                                                                                                                     ccAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAGTCCAGGCTTCGCTATTTTTGGTGCCATCAGCTa                       <  1:80129/250‑1 (MQ=255)                                                                                                                                                                                                                           cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAgg                  >  1:325213/1‑250 (MQ=255)                                                                                                                                                                                                                                          tCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGccc  >  1:100464/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TGATCTTTTTAACAATTCGCTTATCTGTTTCCTGCCAGTACAGATAATCGTCCCATGATTCCTCAGACCAGATTAGTTTCACTCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCC  >  NC_000913/2089384‑2089867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑