Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,887,158	C→A	L278L (CTG→CTT)	rnd ←	RNase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,887,158	0	C	A	100.0%	243.7 / NA	65	L278L (CTG→CTT)	rnd	RNase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/31);  total (34/31)

CTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATC  >  NC_000913/1886912‑1887388                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        cacaccacGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCACTTCGGCGCTGATCTTATGCGTTTCGATCACGTCAGTAATCAGCGACTTAATCGATTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTACGCATCGGCAGCGGTAAGGCCTCTTCCGGCAATGTCTGCGCTTTTTCCACAAgcg                                                                                                                                                                                                                                     >  2:270064/3‑251 (MQ=255)         gCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTagcagc                                                                                                                                                                                                                             >  1:479539/1‑251 (MQ=255)              ccGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTGTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGGGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGtttt                                                                                                                                                                                                                        <  1:161560/251‑1 (MQ=255)               cGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTCAACGCTTTACGATAACCCGGCATGACCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGACTGCGCTTTTTCCACAAGCGCTAGCAGCGtttt                                                                                                                                                                                                                        >  2:109111/1‑250 (MQ=255)                           ccGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg                                                                                                                                                                                                           >  1:78637/1‑251 (MQ=255)                                 aaatGTTCTGCGGTTTCAGGTTCCAGTGCCAGTTCAGCAGTTGGTTGAGTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCg                                                                                                                                                                                                     <  2:456562/248‑1 (MQ=255)                                          gCGGTTTCAGTTTCCAGGGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTCAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGAt                                                                                                                                                                                            <  2:206245/251‑1 (MQ=255)                                            ggTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaa                                                                                                                                                                                          <  1:30496/251‑1 (MQ=255)                                             gTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGCGAAAGCGGATTTCGCTACCGGAtca                                                                                                                                                                                          >  2:54124/1‑248 (MQ=255)                                                 cAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGCTGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAccc                                                                                                                                                                                      >  1:155742/1‑250 (MQ=255)                                                  aGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGNGAAAGCGGATTTCGCTACCGGATAAAccc                                                                                                                                                                                      >  2:231312/1‑249 (MQ=255)                                                   gTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAgg                                                                                                                                                                                   >  1:96685/1‑251 (MQ=255)                                                          gTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGAATAAACCCAGGCTGTCCa                                                                                                                                                                            <  2:419103/251‑1 (MQ=255)                                                            gCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt                                                                                                                                                                          >  2:13494/1‑251 (MQ=255)                                                               aGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCg                                                                                                                                                                       <  2:79452/251‑1 (MQ=255)                                                                gTTCAGCAGTTGGTTGATCTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGc                                                                                                                                                                      >  2:413923/1‑251 (MQ=255)                                                                            gttgATTTGCCGGCTAGATGCCCGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCCTGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTccc                                                                                                                                                           <  2:58681/250‑1 (MQ=255)                                                                                aTTTGCCGACGCGCTGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCACGGCat                                                                                                                                                      >  2:492926/1‑251 (MQ=255)                                                                                aTTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCat                                                                                                                                                      <  1:214880/251‑1 (MQ=255)                                                                                       gACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAAcgc                                                                                                                                               <  2:98213/251‑1 (MQ=255)                                                                                          gcgATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCt                                                                                                                                            >  1:14739/1‑251 (MQ=255)                                                                                             aTGCCAGCAATGCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTAcc                                                                                                                                         <  1:75132/251‑1 (MQ=255)                                                                                                           gcgcTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGAATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATCACGCGCTACCGACCACAAATGctc                                                                                                                           >  1:328034/1‑251 (MQ=255)                                                                                                            cgcTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGctct                                                                                                                          >  1:249156/1‑251 (MQ=255)                                                                                                             gcTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCtt                                                                                                                         >  1:440081/1‑251 (MQ=255)                                                                                                                 aTCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTcacg                                                                                                                     >  1:18620/1‑251 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag                                                                                                                                                                                                                                 <  2:35498/139‑1 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag                                                                                                                                                                                                                                 >  1:35498/1‑139 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTcacgc                                                                                                                    <  1:231312/248‑1 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTcacg                                                                                                                     <  1:13494/247‑1 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTcac                                                                                                                      <  1:182266/246‑1 (MQ=255)                                                                                                                     tATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCAACAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTcacgcac                                                                                                                  <  2:405039/250‑1 (MQ=255)                                                                                                                              cGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTc                                                                                                       <  2:134563/251‑1 (MQ=255)                                                                                                                                     gTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCa                                                                                                <  1:218713/251‑1 (MQ=255)                                                                                                                                           aaTCAGCGACTTAATCGCTTTAAACGCTTTACGATAGCCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATcgc                                                                                          <  1:447864/251‑1 (MQ=255)                                                                                                                                           aaTCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACAGTGAAAGCGGCTTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACACGCTACCGACCACACATGCTCTTCACGCACG‑CACAAGTTCACCGCCAGATcgc                                                                                          >  2:87318/1‑251 (MQ=255)                                                                                                                                                gcgtcTTAATTGTTTTAAACGCTTTACTANAACCCGGCAGTTCCATTAGGTTAAGTATCGGGTGCGGTAAGGCATTTTCCGGCAATGTCTGCGCTTTTGCCCCAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGct                                                                                       <  2:223776/245‑1 (MQ=255)                                                                                                                                                gCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCAGCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCTGTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGctct                                                                                     <  2:351514/251‑1 (MQ=255)                                                                                                                                                  gACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTAGCCTAAACTTCCCGGCATATAACGCGCGACCGCACACAAATGCTCTTCACGCCCGACA‑AAGATAACCGCCAGAGCGCGCcctcc                                                                                   >  2:106441/1‑250 (MQ=255)                                                                                                                                                   aCTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCAGCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGGGAAAGCGGATTTCGCTACCGGATAAACCCAGGCGGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCGCAAAGGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTcgc                                                                                  <  2:55487/251‑1 (MQ=255)                                                                                                                                                        aTCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCtt                                                                             >  1:22547/1‑251 (MQ=255)                                                                                                                                                          cGCTTGAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCGTCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCGCAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACCTCCCGGCATATAACGCGCTACCGACCACAAATGCACTTCACTCACGGCA‑AAGTTCACCGCCAGATCGCGTGCTCGCGCCTTgc                                                                           <  1:270064/251‑1 (MQ=255)                                                                                                                                                           gCTTTAAACGCTTTCCGATAACCCGGCAGGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGACTGCGCTTTTTCCCCAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCTCCGcc                                                                                                 <  2:326703/228‑1 (MQ=255)                                                                                                                                                           gCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCt                                                                                                                                                                                                                                   <  2:365713/99‑1 (MQ=255)                                                                                                                                                           gCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCt                                                                                                                                                                                                                                   >  1:365713/1‑99 (MQ=255)                                                                                                                                                           gCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGcc                                                                                                 >  1:326703/1‑228 (MQ=255)                                                                                                                                                               tAAACGCTTTACGATAACACGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCCGGCTGTCCAGTTCGCCTCAACTTCCCGGCATATAACGCGCTACCGACCACAAATTCTCTTCCCGAACGCCA‑AAGTTCACCGCCCGACCGCGCTCTCGCCCCTTGCGCAGt                                                                      >  2:422993/1‑251 (MQ=255)                                                                                                                                                                      tttACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGt                                                               >  1:153515/1‑251 (MQ=255)                                                                                                                                                                        tACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTc                                                              <  1:131473/250‑1 (MQ=255)                                                                                                                                                                            aTAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGc                                                           <  2:321781/249‑1 (MQ=255)                                                                                                                                                                              aaCCCGGCATGTCCATCAGGTTAAGCAGCGGCAGCGGTAAGGCATCTTCCGACAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGGGAACGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACATCCCGGCATATAACGCGCAACCGACCACAAATGCTCTTCTCGCACGACA‑CAGTTCACCGCAAGATCGCGCCCTCGCGCCTTGCGCAGTCGCCAGTCGGCtca                                                        >  2:268431/1‑248 (MQ=255)                                                                                                                                                                                cccGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCCCCACATCGCGCTCTCGCGCCTCGCGCAGTCGCCCGTCGGCTAAc                                                       >  2:168006/1‑249 (MQ=255)                                                                                                                                                                                          ccATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGAc                                             >  1:314888/1‑249 (MQ=255)                                                                                                                                                                                          ccATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGAc                                             >  2:113573/1‑249 (MQ=255)                                                                                                                                                                                          ccATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCCCGCGCCTTGCNCAGTCGCCAGTCGGCTAACAGTTGCAGAc                                             >  2:243227/1‑249 (MQ=255)                                                                                                                                                                                                   tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTg                                   <  1:492926/250‑1 (MQ=255)                                                                                                                                                                                                        aTCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCgtgt                             >  1:31700/1‑251 (MQ=255)                                                                                                                                                                                                         tcggcttgggTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGGCA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCgtgt                             <  2:450390/242‑1 (MQ=255)                                                                                                                                                                                                          cGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGc                           <  1:171602/251‑1 (MQ=255)                                                                                                                                                                                                          cGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGc                           >  1:81662/1‑251 (MQ=255)                                                                                                                                                                                                              tGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTa                        >  1:102614/1‑250 (MQ=255)                                                                                                                                                                                                                  gTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGc                   >  1:161794/1‑251 (MQ=255)                                                                                                                                                                                                                    aaGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGcc                  <  1:413923/250‑1 (MQ=255)                                                                                                                                                                                                                     aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGcc                  >  1:69804/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaTGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATc  <  1:70096/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        CTCACCACGCCAGCCGGAAATCAGCTCCGGCAAATTGTTCTGCGGTTTCAGTTTCCAGTGCCAGTTCAGCAGTTGGTTGATTTGCCGACGCGATGCCAGCAATTCGGCGCTGATCTTATGCGTTTCGCTCACGTCAGTAATCAGCGACTTAATCGCTTTAAACGCTTTACGATAACCCGGCATGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACA‑AAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATC  >  NC_000913/1886912‑1887388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑