Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,851,841	C→T	Q328* (CAA→TAA)	ansA →	L‑asparaginase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,851,841	0	C	T	100.0%	223.5 / NA	59	Q328* (CAA→TAA)	ansA	L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/27);  total (32/27)

ATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAG  >  NC_000913/1851596‑1852078                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aTGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAAc                                                                                                                                                                                                                                          >  1:231190/1‑251 (MQ=255)     cccACAAAACAACGCCTTCCTGCAGGAATTACAAGAGGCCAGCGATCGCGGGATTGTGGTGGTCCACCTGACACAATGTATGGCCGGTAAAGTGAACATGGTTGGTTATGCCACCGGTAACGCCCTCGCCCTTGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGACGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTgc                                                                                                                                                                                                                                      <  2:390213/250‑1 (MQ=255)          aaaacaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGGGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCgcggc                                                                                                                                                                                                                                 <  1:283786/251‑1 (MQ=255)             acaaaGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGaa                                                                                                                                                                                                                              <  1:136983/251‑1 (MQ=255)                  gCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAAATGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGAc                                                                                                                                                                                                                         >  2:110875/1‑251 (MQ=255)                    cTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGAGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGACAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGc                                                                                                                                                                                                                       >  1:461785/1‑251 (MQ=255)                      tCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAACTAAAACCTGCGCGGCGAACTGACGCt                                                                                                                                                                                                                      <  1:6633‑M1/250‑2 (MQ=255)                       ccTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCttt                                                                                                                                                                                                                    <  1:428719‑M1/251‑4 (MQ=255)                       ccTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtt                                                                                                                                                                                                                     <  1:345710‑M1/250‑3 (MQ=255)                          gCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttg                                                                                                                                                                                                                   <  1:153770‑M1/249‑5 (MQ=255)                           cAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctc                                                                                                                                                                                                                >  1:87828‑M1/1‑244 (MQ=255)                                aTTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATGCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatc                                                                                                                                                                                                           <  1:420263‑M1/251‑13 (MQ=255)                                  tACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctc                                                                                                                                                                                                         >  1:83035‑M1/1‑237 (MQ=255)                                   aCAAGAAGCCAGCGATCGCGATATTGTGGTNGTCAACCTGACCCAATGTATGTCCGGTAAAGTGAACATGGGTTGTGATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctc                                                                                                                                                                                                         <  2:232284‑M1/250‑15 (MQ=255)                                         agCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcccttta                                                                                                                                                                                                   >  2:212872‑M1/1‑230 (MQ=255)                                          gCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaat                                                                                                                                                                                                 >  1:421417‑M1/1‑229 (MQ=255)                                               cGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgc                                                                                                                                                                                            <  1:110875‑M1/251‑28 (MQ=255)                                                 aTCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctc                                                                                                                                                                                          >  1:27149‑M1/1‑222 (MQ=255)                                                              ggtggtCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgggact                                                                                                                                                                             >  2:64209‑M1/1‑209 (MQ=255)                                                               gtggtCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactg                                                                                                                                                                            >  1:323441‑M1/1‑208 (MQ=255)                                                                     aaCCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattct                                                                                                                                                                       >  1:247017‑M1/1‑202 (MQ=255)                                                                      aCCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaa                                                                                                                                                                     <  1:212872‑M1/251‑51 (MQ=255)                                                                               aaTGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGACCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCAAGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctccctttaatcgtgctcacattacgtgactgattctaacaaaacatt                                                                                                                                                            >  1:476780‑M1/1‑192 (MQ=255)                                                                                atgtatgtCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacatta                                                                                                                                                           <  1:64209‑M1/251‑61 (MQ=255)                                                                                atgtatgtCCGGGAAAGGGAACATGGGGGGTTATGCCAACGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGAATGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacatgacgtgactgattctaacaaaacagta                                                                                                                                                           <  2:474468‑M1/251‑61 (MQ=255)                                                                                  gtatgtCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaac                                                                                                                                                         <  1:252778‑M1/251‑63 (MQ=255)                                                                                  gtatgtCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGAGCAGATATGACTGTAGAAGCCCCGCTAACCCAACTGCATTACCTGCTTAACCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaac                                                                                                                                                         >  1:456912‑M1/1‑189 (MQ=255)                                                                                     tgtCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcaatttaatcgtgctcacattacgtgactgattctaacaaaacattaacacc                                                                                                                                                      >  1:356612‑M1/1‑186 (MQ=255)                                                                                       tCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacacca                                                                                                                                                     >  1:171488‑M1/1‑184 (MQ=255)                                                                                          ggTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactg                                                                                                                                                 <  1:243379‑M1/251‑71 (MQ=255)                                                                                             aaaGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggc                                                                                                                                               >  1:151356‑M1/1‑178 (MQ=255)                                                                                              aaGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCAATCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggca                                                                                                                                              >  1:259473‑M1/1‑177 (MQ=255)                                                                                                  gAACATGTGTGGTTATGCCACCGGTAACGCCCTTTCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTCCTTAGCCAGTAGCTGGATACTGAAACCATTCGCAAGGCCATGTACTAAAACCGGCGCGGCGAACTGACGCtttactcacagctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaacgggcaaaatt                                                                                                                                         <  2:287302‑M1/251‑79 (MQ=255)                                                                                                  gAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACCGTAGAAGCCACGCCAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGAAGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaatt                                                                                                                                         >  1:301545‑M1/1‑173 (MQ=255)                                                                                                   aaCATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattt                                                                                                                                        >  1:375504‑M1/1‑172 (MQ=255)                                                                                                                aTGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttg                                                                                                                           <  1:162553‑M1/251‑93 (MQ=255)                                                                                                                aTGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttg                                                                                                                           <  2:347159‑M1/251‑93 (MQ=255)                                                                                                                  gCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgat                                                                                                                         >  1:42284‑M1/1‑157 (MQ=255)                                                                                                                             cGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctatacttgatctcgacgaaat                                                                                                              <  2:421417‑M1/251‑106 (MQ=255)                                                                                                                              gCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctaacatctcactttaatcgtgatcacattacgtgaatgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatg                                                                                                             >  1:63826‑M1/1‑145 (MQ=255)                                                                                                                                    gCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaa                                                                                                                                                                 <  1:443363‑M1/193‑55 (MQ=255)                                                                                                                                    gCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaa                                                                                                                                                                 >  2:443363‑M1/1‑139 (MQ=255)                                                                                                                                                         ggTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatc                                                                                   >  1:279206‑M1/1‑118 (MQ=255)                                                                                                                                                             cAGATATGACTGTAGAAGCCACGCTAACCAANCTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatt                                                                              <  2:247017‑M1/251‑138 (MQ=255)                                                                                                                                                                  atGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacattttta                                                                          <  1:263321‑M1/250‑142 (MQ=255)                                                                                                                                                                                cACGCTAAACAAACGGCATTACCTGCTTAGCCAGGAACTTGATACTGAAACCATTCGCAAGTCCATGAGCTAAAACCTGCGCGGCGATCTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactgtcaaaattttgtcctaaacttgatctcgtcgaaatggctgcacctaaatcttgatgaaaatcacatttgtatcgtaattgcccttt                                                           <  2:459550‑M1/251‑157 (MQ=255)                                                                                                                                                                                         cAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcaca                                                                                                                                                                                       >  2:383106‑M1/1‑86 (MQ=255)                                                                                                                                                                                         cAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcaca                                                                                                                                                                                       <  1:383106‑M1/118‑33 (MQ=255)                                                                                                                                                                                               gCATTACCTGCTTAGCCCGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaaaacaaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgggatgaaaatcacatttttatcgtatttgccctttaaacttcggggcgcc                                            >  2:470433‑M1/1‑80 (MQ=255)                                                                                                                                                                                                        gCTTAGCCAGGAACTGGCTACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgac                                                                                                                   >  2:38080‑M1/1‑71 (MQ=255)                                                                                                                                                                                                        gCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgac                                                                                                                   <  1:38080‑M1/171‑101 (MQ=255)                                                                                                                                                                                                           tAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgt                                  <  2:323441‑M1/249‑182 (MQ=255)                                                                                                                                                                                                              ccAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactggttctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtct                             >  1:289264‑M1/1‑65 (MQ=255)                                                                                                                                                                                                                    aCTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctctacgaaatggctgcacctaaatcgtgatgaaaatcacatttttat                                                                         <  2:378946‑M1/201‑143 (MQ=255)                                                                                                                                                                                                                    aCTGGATACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttat                                                                         >  1:378946‑M1/1‑59 (MQ=255)                                                                                                                                                                                                                          tACTGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaagg                 >  2:420222‑M1/1‑53 (MQ=255)                                                                                                                                                                                                                             tGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgncgaccccatgtggtctcaagcccaaagga                >  2:239074‑M1/1‑50 (MQ=255)                                                                                                                                                                                                                             tGAAACCATTCGCAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccaaagga                >  1:229877‑M1/1‑50 (MQ=255)                                                                                                                                                                                                                                         cAAGGCCATGAGCTAAAACCTGCGCGGCGAACTGACGCtttgctcacatctcactttaatcgtgctcacattacgtgactgattctaacaaaacattaacaccaactggcaaaattttgtcctaaacttgatctcgacgaaatggctgcacctaaatcgtgatgaaaatcacatttttatcgtaattgccctttaaaattcggggcgccgaccccatgtggtctcaagcccacaggaagagtgaggcgagt  >  1:316932‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTGTGGTGGTCAACCTGACACAATGTATGTCCGGTAAAGTGAACATGGGTGGTTATGCCACCGGTAACGCCCTCGCCCATGCCGGCGTAATTGGCGGTGCAGATATGACTGTAGAAGCCACGCTAACCAAACTGCATTACCTGCTTAGCCAGGAACTGGATACTGAAACCATTCGCAAGGCCATGAGCCAAAACCTGCGCGGCGAACTGACGCCGGATGATTAAGGAGACTGTAATGCCCCCTCGCGCCCTGTTACTGGTCGATTTACAAAATGATTTCTGTGCTGGTGGCGCGCTCGCCGTGCCGGAAGGTGACAGTACGGTGGATGTCGCTAACCGCCTGATTGACTGGTGCCAGTCGCGCGGTGAAGCGGTTATCGCCAGTCAGGACTGGCACCCGGCGAATCACGGCAGTTTTGCCAGTCAG  >  NC_000913/1851596‑1852078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑