Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,413,450	C→T	M1M (ATG→ATA) †	xisR ←	excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413,450	0	C	T	100.0%	197.2 / NA	53	M1M (ATG→ATA) †	xisR	excisionase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/29);  total (24/29)

CTAATCTCGACACCTGTTGGTAATTTAGACATATCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGT  >  NC_000913/1413204‑1413695                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      caaaTCTCGACACCTTTTGGTAATTTAGACATAGCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCTAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATc                                                                                                                                                                                                                                                    <  2:5931/248‑1 (MQ=255)        cGACACCTGTTGGTAATTTAGACATATCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCAcc                                                                                                                                                                                                                                            >  2:176084/1‑251 (MQ=255)                               atgtCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGGTTGAAGTGGAAGCCCTCCGCCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGAc                                                                                                                                                                                                                     <  1:164665/248‑1 (MQ=255)                               atatCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGAc                                                                                                                                                                                                                     <  1:464254/251‑1 (MQ=255)                                 atCATGAGTTTTTGATAAGCTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCTACCCAACAGTTCTGGCGGTATGGTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCAGCC‑TCTCTTCATTAAAGATTACTTGTGCTATCTCATCACCTCTGGCAGTCGCCAATGTTAGACt                                                                                                                                                                                                                    <  2:282266/250‑1 (MQ=255)                                    aTGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGaa                                                                                                                                                                                                                 >  1:259284/1‑250 (MQ=255)                                     tGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGc                                                                                                                                                                                                                                                  <  1:130628/216‑1 (MQ=255)                                     tGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGc                                                                                                                                                                                                                                                  >  2:130628/1‑216 (MQ=255)                                      gAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAAtt                                                                                                                                                                                                              <  1:23784/251‑1 (MQ=255)                                            ttGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGcc                                                                                                                                                                                                        >  2:436231/1‑251 (MQ=255)                                                tAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCTTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGATGCATCAGGCCGTATTCAACCATCC‑GCTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGa                                                                                                                                                                                                                      <  2:294613/233‑1 (MQ=255)                                                tAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCACTTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCCATGTTAGa                                                                                                                                                                                                                      >  1:294613/1‑233 (MQ=255)                                                      gATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTg                                                                                                                                                                                                <  1:376971/249‑1 (MQ=255)                                                                 ggATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGACCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATc                                                                                                                                                                                   >  1:401791/1‑251 (MQ=255)                                                                        tGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATg                                                                                                                                                                            >  2:73696/1‑251 (MQ=255)                                                                         gTACCAGATAATCCCTGGTTTGCTGTCTGGCTTACCTAAATGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTAGGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCGTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGa                                                                                                                                                                           <  2:200043/251‑1 (MQ=255)                                                                               gATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGtttt                                                                                                                                                                     >  1:331329/1‑251 (MQ=255)                                                                                   agcccTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAAtt                                                                                                                                                                 <  1:159262/249‑1 (MQ=255)                                                                                    tCCCTCGTTTGCTGTCTGGCTTACCTCAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGTTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTAGACTTGACGCCCGATGTTGATTATTAATAATCAGATATGAAGTTTTAAttt                                                                                                                                                                >  1:142009/1‑251 (MQ=255)                                                                                          gTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATAc                                                                                                                                                          <  1:73696/251‑1 (MQ=255)                                                                                                     ggCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATg                                                                                                                                                                                                   >  2:204523/1‑199 (MQ=255)                                                                                                     ggCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAAtt                                                                                                                                                 <  2:208319/249‑1 (MQ=255)                                                                                                     ggCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAAt                                                                                                                                                  <  1:230331/248‑1 (MQ=255)                                                                                                     ggCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCAGCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATg                                                                                                                                                                                                   <  1:204523/199‑1 (MQ=255)                                                                                                      gCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATtctc                                                                                                                                              <  1:414536/251‑1 (MQ=255)                                                                                                       cTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCTCTCCACCCAACAGTTCTGGCGGAATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCCCAC‑CGTCATTAAAGATTACTTGTGCTATCGAATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTAACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCg                                                                                                                                             >  2:377395/1‑251 (MQ=255)                                                                                                        ttACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCg                                                                                                                                             >  1:426900/1‑250 (MQ=255)                                                                                                         tACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCCCCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCg                                                                                                                                             <  2:87247/249‑1 (MQ=255)                                                                                                               aaGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGa                                                                                                                                     <  1:436231/251‑1 (MQ=255)                                                                                                                    gaTACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACCCCCGCTGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGttt                                                                                                                                >  2:42209/1‑251 (MQ=255)                                                                                                                       aCTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTtctc                                                                                                                             >  2:296066/1‑251 (MQ=255)                                                                                                                         cttccgatcttGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGc                                                                                                                           >  2:119833/11‑251 (MQ=255)                                                                                                                          cgttttaaggcgaatcgcTCCCCCCAACANTTCGGGCTGTATTGTTCAATTTGGCGGACCCCCAGACCAGTGGGAAGCATCAGGCCGTATTCATCCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCTCGATGTTGATTATTAATAATCAGCTATGAAGTTTTGATTTGAATACAATGCAATTCTCGATGACTGAAGTTtcacg                                                                                                                            <  2:230315/232‑4 (MQ=255)                                                                                                                             ttGAAGTGGAAGCCCTCCACCCAACAGTGCTGGCTGTATGCTTCAATTTGGCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGTCTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCGCGATGACTGAAGTTTCTCGCAAt                                                                                                                        <  1:443438/250‑1 (MQ=255)                                                                                                                              tGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAAtt                                                                                                                       <  1:361588/250‑1 (MQ=255)                                                                                                                                aaGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTaaa                                                                                                                    <  1:373485/251‑1 (MQ=255)                                                                                                                                    ggAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCGTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAAtt                                                                                                                 <  2:137536/250‑1 (MQ=255)                                                                                                                                                        ttCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctc                                                                                            >  1:413127/1‑251 (MQ=255)                                                                                                                                                            ggCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTg                                                                                        <  1:296066/251‑1 (MQ=255)                                                                                                                                                                  aTGCTTCAATTTGTATGGACCCCAGACAAGTGCGAAGCATCAGGCCGTATTAAACCATCC‑ACTCTTCATTCAAGATTACTTGTGCTATCGCATCACCTCTGGCAGTCGCCAATGTTAGACTGCAATTGACGCCCGATGTTGATTTTTAATAATGAGCTCTGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGATGTTTCTCGCAATTAAATTTTATCAGTTTTCCTTTCTGCTCTCTGGAAAct                                                                                  >  2:392102/1‑250 (MQ=255)                                                                                                                                                                       tCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGc                                                                             >  2:379944/1‑251 (MQ=255)                                                                                                                                                                       tCAATTTGTCCAGCCCCCAGACCAGTACGCAGCATCAGGCCTTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATGCTCGATGACGGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCGCTCTGGAATCGCCTg                                                                              <  2:307198/250‑1 (MQ=255)                                                                                                                                                                            ttGTCTGGCCCCCAGACCAGTGCGAGGCATCAGGCCGTATTCATCCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATGAATAAGCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACGGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGcttct                                                                         <  2:325857/250‑1 (MQ=255)                                                                                                                                                                                         aGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGagag                                                           <  1:99054/251‑1 (MQ=255)                                                                                                                                                                                                  cGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTc                                                  <  1:176084/251‑1 (MQ=255)                                                                                                                                                                                                                 tATTCAACCATCA‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTANCTGAGAGCATTTTTTCGCATTCTGATTTCGt                                   >  2:228131/1‑251 (MQ=255)                                                                                                                                                                                                                         cATCC‑ACTCTTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTGCCTTAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAg                           <  2:426900/251‑1 (MQ=255)                                                                                                                                                                                                                                  ctTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTg                                                               >  1:78946/1‑207 (MQ=255)                                                                                                                                                                                                                                  ctTCATTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTg                                                               <  2:78946/207‑1 (MQ=255)                                                                                                                                                                                                                                      aTTAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGACATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAACATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTCGTTTAGATTTTGAATATc               >  1:475143/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttAAAGATTACTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCt              >  1:239421/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cTTGTGCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGtt     >  2:406505/1‑250 (MQ=255)                                                                                                                                                                                                                                                    gtgCTATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGATGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTtagt  >  1:472617/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTAATCTCGACACCTGTTGGTAATTTAGACATATCATGAGTCTTTGATAAACTGATTTATCTTTGGATAGTTGTACCAGATAATCCCTCGTTTGCTGTCTGGCTTACCTAAAGGAGATACTCGTTTGAAGTGGAAGCCCTCCACCCAACAGTTCTGGCGGTATGCTTCAATTTGTCTGGCCCCCAGACCAGTGCGAAGCATCAGGCCGTATTCAACCATCC‑ACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGT  >  NC_000913/1413204‑1413695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑