Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,852	T→C	K244R (AAG→AGG)	lpxL ←	lauroyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,852	0	T	C	100.0%	235.9 / NA	68	K244R (AAG→AGG)	lpxL	lauroyl acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/33);  total (35/33)

CTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATCGATTTATTTGAGCGCAAACGGCCCCAGGTTTGTAGCC  >  NC_000913/1115616‑1116095                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    gtgtgGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg                                                                                                                                                                                                                                       <  2:116355/250‑1 (MQ=255)   gtgGTAATGCCGGAAGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGCGAACCCCCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAcca                                                                                                                                                                                                                                      >  2:469894/1‑248 (MQ=255)     ggTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAgg                                                                                                                                                                                                                                   <  1:390943/251‑1 (MQ=255)         aTGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGGGGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACGGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATACAGTGGCGGAGAACACACTTGCGGCAGCATAATCAA‑TTGATAGCCTTTTCCATCTGGCCTACGGCGTGGAACGAAgggtc                                                                                                                                                                                                                                >  1:271426/1‑248 (MQ=255)           gCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAcc                                                                                                                                                                                                                              <  1:480706/250‑1 (MQ=255)                  gCCATTCTGAAGCATCCGGCATGGGAGATTTNATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATgc                                                                                                                                                                                                                      <  2:229396/251‑1 (MQ=255)                     aTTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGcc                                                                                                                                                                                                                   <  1:360822/251‑1 (MQ=255)                     aTTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGACGGTAGTTTCGGCATCATCCAGTGGCGGATAACACTCTGGCGGCCACATACTCAA‑TTGATACACTTTGCCCTCTGGCCACCGGCGTGGacagaacggaccccaagaagcgcc                                                                                                                                                                                                                   >  2:312005/1‑228 (MQ=255)                       tCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCg                                                                                                                                                                                                                  <  1:486883/250‑1 (MQ=255)                           aaGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACAACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCCTCCAGTGGCGGAGAACAATCTGACGGACGCAATATCAA‑TTGATACCCTTTGCCATCTGGCCTACAGCGTGGCACGAAGGGCACCAGCAACGCGCCGGACAt                                                                                                                                                                                                             >  2:380385/1‑251 (MQ=255)                             gCATCCGGCATGGGAGATTTAATAGCGTGAAGTAACGCCTTTCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTGTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGTCACCAGACATGCGCCGGACATcc                                                                                                                                                                                                           <  1:478921/251‑1 (MQ=255)                                tCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCAGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTg                                                                                                                                                                                                        >  1:91748/1‑251 (MQ=255)                                 ccGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCAGCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTg                                                                                                                                                                                                        <  2:24242/250‑1 (MQ=255)                                     cATGGGAGCTTTCATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAAAAATCTGGCGGCAGCATAAACAAATTG‑TACCCTTTTACATCTGGCCTAAGGCGGGGGACGAAGGGCACCCGACATGCGCCCGGCATCCGTGcccac                                                                                                                                                                                                   >  2:284545/1‑248 (MQ=255)                                        gggAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCCTCATCCAGTGGCGGAGAAAACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCCGGCCTACGGCGTGGAACGAAGGGCACCAGACCTGCGCCGGACATCCGttcccgcaac                                                                                                                                                                                                >  1:480049/1‑242 (MQ=255)                                            gaTTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCCGTGGCGGAAAACACTCTGGAGGCAGCATAATCAA‑TTTATACCCTTTGCCATCCGGCCTCCGGCGCGGAACGACGGGGACCCGACATACGCCGGCCAACCCTGCCCTCATCCAgg                                                                                                                                                                                            >  2:289324/1‑251 (MQ=255)                                               ttAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCCGGTTc                                                                                                                                                                                         >  1:26140/1‑251 (MQ=255)                                                    aGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCCTCCAGGTTCCGGTc                                                                                                                                                                                    >  1:210605/1‑251 (MQ=255)                                                             gAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTAAGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGGTCCGGTCGTGGTCGc                                                                                                                                                                            >  1:322455/1‑250 (MQ=255)                                                                 gCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCt                                                                                                                                                                       <  1:226313/251‑1 (MQ=255)                                                                 gCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACACGACATGCGCCGGAAATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCt                                                                                                                                                                       >  1:304295/1‑251 (MQ=255)                                                                  ccTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTg                                                                                                                                                                      <  2:73758/251‑1 (MQ=255)                                                                  ccTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACCAAGGGCACCAGACATGCGCCGGAAATCCCTGCCAGCATCCCGGTTCCGGGCGGGGGCGCAGcccg                                                                                                                                                                      >  2:200647/1‑249 (MQ=255)                                                                    ttCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGGGTCAGCATCCAGGATCCGGGCGGGGTCGCaacccgct                                                                                                                                                                    >  1:313199/1‑244 (MQ=255)                                                                              gtTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTTCCATCCGGCCTACGGCGTGGAACGAAAGGCACCAGACATGCGCCGGGCATCCGGGCCAGCCTCCCGGGTCCGGTCGTGGTCGCCGCCCACTCACCGGCaa                                                                                                                                                           >  2:279902/1‑250 (MQ=255)                                                                                tttAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAaac                                                                                                                                                         <  1:279902/250‑1 (MQ=255)                                                                                     aGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACggg                                                                                                                                                   <  1:475467/251‑1 (MQ=255)                                                                                       cGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTGTCGACCACTGTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGAc                                                                                                                                                 <  2:210605/251‑1 (MQ=255)                                                                                         aCGGTGTAACCACATATACTGCTCTGCTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAAGAAGGGCACCAGGCACGCGCCCGACATCCGTGCCAGCATCCAGGGTCCGGTCGGGGTCGAAGCCTGCTCAACGGCAAACAACGGGACg                                                                                                                                                >  1:146685/1‑250 (MQ=255)                                                                                           ggTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGaaa                                                                                                                                             <  2:26140/251‑1 (MQ=255)                                                                                            gtgtAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGGCGTGGTCGCAGCCTGCTCAACGGCAAACACCGGGACGaaa                                                                                                                                             >  1:18672/1‑250 (MQ=255)                                                                                                 aCCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTATTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCtt                                                                                                                                       <  2:91748/251‑1 (MQ=255)                                                                                                    acaTATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAg                                                                                                                                    <  1:341415/251‑1 (MQ=255)                                                                                                             gCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGc                                                                                                                             <  1:323986/249‑1 (MQ=255)                                                                                                             gCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCg                                                                                                                           >  1:226892/1‑251 (MQ=255)                                                                                                                     gCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCCTCTGGCCTACGGCGTGGAACGAAGGGCCCCAGACAAGCGCCGGACATCCCTGCCAGCATCCAGGTTCCGGTCGGGGGCCCAGCCTGCTCACAGGGAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTCATCATg                                                                                                                   >  2:399864/1‑251 (MQ=255)                                                                                                                     gCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATg                                                                                                                   <  1:108967/251‑1 (MQ=255)                                                                                                                       catcatGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGa                                                                                                                  >  1:3497/1‑250 (MQ=255)                                                                                                                                gCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGGGGAACGAAGGGCACCAGACCTGCGCCGGACCTCCGGGCCAGCATCCAGGGTCCGGTCGTGGTCGCAGCCTGCTCAACGGGAAACAACGGGGCGGAAACGCCTGGGCGCGGGCCGGAAACATgtaccggggcgg                                                                                                        >  2:117892/1‑240 (MQ=255)                                                                                                                                  aTTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTAc                                                                                                      <  1:200647/251‑1 (MQ=255)                                                                                                                                           ccACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACCAAGGGCACCAGACAAGCCCCGGACATCCGTGCCAGCATCCAGGGTCCGGTCGTGGTCGCAACCCGGTCCACGGCCAACAACGGGACGCAAACGCCTGAGCGCGGGCCGTAATCATTATCCGGTGCGTACCCGAccccc                                                                                             >  2:196515/1‑248 (MQ=255)                                                                                                                                                  gTTCATCCCCGCGGCGGTAGTTTCGCCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATTCCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCTTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACTCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCt                                                                                       <  1:174842/250‑1 (MQ=255)                                                                                                                                                     cATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCtttt                                                                                    <  1:117892/250‑1 (MQ=255)                                                                                                                                                        ccccgcgGCGGTAGTTCCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCCGCATACTCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGGGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAAGCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCa                                                                                <  2:313788/248‑1 (MQ=255)                                                                                                                                                         cACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGGCGTGGGCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACCTCCCCTTTTTTc                                                                                 >  1:254044/1‑249 (MQ=255)                                                                                                                                                             cggcggTAGTTTCGGCATCATCCAGTGGCGGAGAACCCTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCTTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCt                                                                           <  1:262371/251‑1 (MQ=255)                                                                                                                                                                 ggTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCCGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGGGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTa                                                                        >  1:124413/1‑250 (MQ=255)                                                                                                                                                                   tAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑ATGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGAATCCAGGTTCCGGTCGGGGTCGCCGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCCAGAACCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGATTTAATc                                                                     >  1:49145/1‑251 (MQ=255)                                                                                                                                                                    aGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATc                                                                     <  1:265095/250‑1 (MQ=255)                                                                                                                                                                        tCGGCATCATCCAGTGGCGGAGAACACTCGNGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGCCATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGc                                                                 <  2:233559/250‑1 (MQ=255)                                                                                                                                                                          ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATgc                                                                                                                                                                                                                      <  2:220974/99‑1 (MQ=255)                                                                                                                                                                          ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATgc                                                                                                                                                                                                                      >  1:220974/1‑99 (MQ=255)                                                                                                                                                                               catcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTGGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTaa                                                          <  1:196515/250‑1 (MQ=255)                                                                                                                                                                                     gTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTTCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAGCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTg                                                   <  1:317108/251‑1 (MQ=255)                                                                                                                                                                                      gggCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGc                                                  <  1:153311/250‑1 (MQ=255)                                                                                                                                                                                           gagaACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAAGGCACCAGACATACGCCGGACAGCCGTCCCTACAACCAGGCTCCGGTCGTGGACGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCCCTTCCCCTTTTTTCAGGCCTTTAATCATGCATTTTAACACTTTGCGGTCg                                             >  2:302827/1‑251 (MQ=255)                                                                                                                                                                                            agaACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCg                                             >  1:330050/1‑250 (MQ=255)                                                                                                                                                                                                 cctctGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGTACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATc                                       <  1:227876/250‑1 (MQ=255)                                                                                                                                                                                                         ggcAGCATAATCAA‑TTGATACCCTTTGCAATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACCTGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTNTTAAATCTTTGCGGTCGAGCATCGatttatt                               >  2:239322/1‑251 (MQ=255)                                                                                                                                                                                                           cagcaTAATCAA‑TTGACACCCGTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCCTCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGTTCGAGCATCGATTTATTTg                             <  2:123676/251‑1 (MQ=255)                                                                                                                                                                                                              caTACTCAA‑TTGATACCCTTTGCCATCTGCCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACCTCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCCGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCCTCGATTTATTTGAgc                          >  2:310664/1‑251 (MQ=255)                                                                                                                                                                                                                         tGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATCGATTTATTTGAGCGCAAACGGcc                >  1:333288/1‑251 (MQ=255)                                                                                                                                                                                                                            tACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATCGATTTATTTGAGCGCAAACGGccccc             >  1:448945/1‑250 (MQ=255)                                                                                                                                                                                                                             aCCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTc                                                                                           >  1:223561/1‑172 (MQ=255)                                                                                                                                                                                                                             aCCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTc                                                                                           <  2:223561/172‑1 (MQ=255)                                                                                                                                                                                                                                      cATCTGGCCTCCGGCGTGAAACGAAGGGCCCCAGACATGCGCCGGACATCCGTGCCAGCTTCCAGGTTCCGGTCTTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGGGCATCGATTTATTTGAGCGCAAACGGCCCCAGGTTTGTAGc   <  2:280898/251‑1 (MQ=255)                                                                                                                                                                                                                                        tCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGACGAGCATCGATTTATTTGAGCGAAAACGGCCCCAGGTTTGTAGcc  >  1:326549/1‑250 (MQ=255)                                                                                                                                                                                                                                         cTGGCCTACGGCGTGGAACGAAGGGAACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATCGATTTATTTGAGCGAAAACGGCCCCAGGTTTGTAGcc  >  1:355715/1‑249 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    CTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAA‑TTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGTCGCAGCCTGCTCAACGGCAAACAACGGGACGAAAACGCTTGAGCGCGGGCCGTAATCATGATCCGGTGCGTACCAGACCACTTCGCCTTTTTTCAGGGCTTTAATCATGCCTTTTAAATCTTTGCGGTCGAGCATCGATTTATTTGAGCGCAAACGGCCCCAGGTTTGTAGCC  >  NC_000913/1115616‑1116095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑