Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	160.0 / NA	51	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/21);  total (30/21)

TTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674277‑674762                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ttcgttcgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGCGCGGTGTTGTTTTTCACCGCCACGCCTTCCg                                                                                                                                                                                                                                             >  2:365630/1‑251 (MQ=255)   cgttcgCCAGTACGGTCTGGTCTTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAC‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCgc                                                                                                                                                                                                                                            <  1:265253/250‑1 (MQ=255)       cgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                        <  1:2176/250‑1 (MQ=255)           aGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGAcc                                                                                                                                                                                                                                   >  1:151649/1‑251 (MQ=255)                      gTCGTTCGGGCACCAGTTGACCGCAAAAGTTTTTTTATATCCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTTTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGGGCTGTTTTTTCATATCCGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGTTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTccc                                                                                                                                                                                                                        <  1:482752/251‑1 (MQ=255)                       tCGTTCGGGCACCAGTTGAACGCAGAAGTCTTCTTATATACCAGGCATTTTTTATACAGCCCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGcccc                                                                                                                                                                                                                        >  1:188884/1‑250 (MQ=255)                         gTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc                                                                                                                                                                                                                     >  1:8253/1‑251 (MQ=255)                             gggCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCCTTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGac                                                                                                                                                                                                                 >  2:207502/1‑250 (MQ=255)                              gcgcccAGTTGCCCGCAAAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGTGAAGATTTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATACCCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCTTCCCAGCCGATc                                                                                                                                                                                                                <  1:162307/247‑1 (MQ=255)                                       tGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATCCAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAgg                                                                                                                                                                                                       >  2:204636/1‑251 (MQ=255)                                               gAAGTTTTCTTATATACCAGGCTTTTTTTATACAGCTGGGTGAAAATTTTCTGTCCCAAA‑CGGA‑AGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCATTC‑ATAACCAAAGCCCAGCTTTTTTAGTTGGTTTTTCAAATGCGCGATGTTG‑TC‑GTACTTCCACGGTGCCGGAGCGGTTTTTTTTTTCTCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGttttt                                                                                                                                                                                               <  1:474764/251‑1 (MQ=255)                                                    cttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTCCAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGCGGTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAACCGCGTCCCAGCCGAGCGGCTGCAGGACGGTTTTGcac                                                                                                                                                                                           >  2:172767/1‑248 (MQ=255)                                                        ttacataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGTGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACTTCCACGGTGCCGGAGCGGGGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGAATCCCAACCGCTAGGCTGCAGCACGGTTTTGCCCAGCAt                                                                                                                                                                                      >  2:85599/5‑251 (MQ=255)                                                                      ttttttATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                                                                                                                        >  1:483292/1‑251 (MQ=255)                                                                       tttttttacaccccgttgaggatgttCTGTTCCCAT‑CGGT‑AGTTTCCCGGCGTCCAGTTTGCCCGCTCGCTGCTCCA‑TCTATAACCAAACCCCGTCTTTTTGAGCTGGTTTTTCATTTACGGTTTTTTG‑TC‑GTCCGCCCACGGTCCCGTAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                                                                                                                        <  1:58351/226‑1 (MQ=255)                                                                         tttataatgcgcGGTGAAGAATTTTTGT‑CCCAATCGTT‑AGTATTCGGGCGTACATGTTCCCAGCCCGCGCCCCCAGTC‑ATACCCAAAGCCCAGCATTTTGAGATGGTTTTTCATATCCGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCg                                                                                                                                                                      <  2:151649/239‑1 (MQ=255)                                                                           tataCAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACGGGTTGCCGGCCCGCGGCTCCAGTC‑ATACCCAAAGCCCAGCATTTTGAGCTGTTTTTTCATATCCGCGATGTTG‑TC‑TTACGTCCCCGGTGCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                    <  1:207502/250‑1 (MQ=255)                                                                              aCAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCAc                                                                                                                                                                 <  1:75844/250‑1 (MQ=255)                                                                               tagatCGGTGACGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTAGCGGCTCCAGTC‑ATAACCAAAGCCCCGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCACCCGCGCCCCCGCCGATCGGCTGCAGGACGTTTTTGCACAGCATACGCTGGTAGCGGGCGATCACg                                                                                                                                                                >  2:484245/5‑250 (MQ=255)                                                                                aGCTCGGTGAAGAATTTCTGTTCCCAA‑AGGT‑AGTATTACGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAACGCCCAGCCTTTTGGGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTACCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAACATACGCTGGTAGCGGGCGAGCACGTc                                                                                                                                                              >  2:167812/1‑251 (MQ=255)                                                                                    gggggaagtaTTTCTTTTCCCAA‑AGGTTAGTTTTCCGCCTTACAGGTGCCCACCTCGGGGCCCCAGTA‑AAAACAAAACCCCAGAATTTTGGGCGGTTTTTTAATATCCGCGATGTTT‑TC‑GTCCGTCCGGGGTGCCGGGGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                                                                                                                           <  2:387072/247‑1 (MQ=255)                                                                                                     gtcccccccgggagtaTCCCGGCGTCCGGGTGGCCAGCTCGCGGCTCAAGAC‑TAAACAAAAGCCCAGATTTTTGAGCGGTTTTTTCTTATCCGCGTGGTGG‑TC‑GTACGCCCCCGGTGCGGGGGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                                                                                                                           <  2:100524/239‑1 (MQ=255)                                                                                                        cAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTg                                                                                                                                      >  1:399222/1‑251 (MQ=255)                                                                                                                       ccGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCCGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAGCACGTCACCGATGGGGTATTTACGTACGTGGCCCAtgagtcgt                                                                                                                         >  2:96322/1‑245 (MQ=255)                                                                                                                         ggCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                                                                                                                       >  2:169589/1‑251 (MQ=255)                                                                                                                                         gCTCGCGGTGCCAGTA‑ATAACCAAAGCCCAGCTTTTTGAGCTGGTTTTACATATACGGGATGT‑G‑TCCGTACGCCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGa                                                                                                       <  2:29503/251‑1 (MQ=255)                                                                                                                                            cgcgGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc                                                                                                                                                                                                             >  1:403696/1‑146 (MQ=255)                                                                                                                                             gcgGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAAGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTAAGTGGACCCTGTGTAGTCGTCCAGAAGGCTAGGGAAGc                                                                                                    >  1:396435/1‑250 (MQ=255)                                                                                                                                                cctcaaGTC‑ATACCCAAAGCCAGGCATTTTGGGCTGGTTTTTCATATACGCG‑TGTTGGTC‑GTCCGCCCCCGGGGCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAg                                                                                                <  1:208491/246‑1 (MQ=255)                                                                                                                                                   ccAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGAc                                                                                              >  1:272686/1‑250 (MQ=255)                                                                                                                                                      gTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGGCACCGATGGTGTAGTTACGTACGTGGCCCATGGGTAGTCGACCAGAAGGATAGGGAAGCATAGCCAGGc                                                                                          >  2:393829/1‑251 (MQ=255)                                                                                                                                                          aTAACCAAAGCCCAGCATTTTGCGCGGGTTTTTCATATCCGGGGTTTGG‑TC‑GACCGCCCCGGGGGGCGGGGGGGTTTTTTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGt                                                                                       <  2:30397/251‑1 (MQ=255)                                                                                                                                                            aaCCAAAGCCCAGCATTTTGAGCTGGTTCTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGGTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCCCCGATGGTGTAGTTACGTATGTGGCCCATGTGTAGTCGAACAGACGGGTAGGGAAGCATAGACAGGCAGTa                                                                                      >  2:190144/1‑250 (MQ=255)                                                                                                                                                             cccaaatccaaccATTTTGGGCGGGTTTTTCAATTCCGCGATGTTC‑TC‑CTACGTCCACGGTGCCGGCGCGGGGTTTTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCTGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGGCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAAt                                                                                    <  1:167812/239‑1 (MQ=255)                                                                                                                                                               caattcccAGCTTTTTTAGCTGTTTTTTTTTATCCGCGACTTTG‑TC‑GTCCTTCCCCGGTGCCGGACCGGTGTTTTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAc                                                                                  <  1:482046/246‑1 (MQ=255)                                                                                                                                                                     ccAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATNGGGAAGCATAGACAGGCAGTAATACTTctct                                                                            >  2:229467/1‑251 (MQ=255)                                                                                                                                                                                    ggTTTTTCATATCCGCGTTGTTG‑TC‑GTACGCCCACGGTGCCGGAGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTc                                                              <  1:82683/250‑1 (MQ=255)                                                                                                                                                                                       ttttCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGtt                                                          >  1:199292/1‑251 (MQ=255)                                                                                                                                                                                            atatACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTc                                                     >  1:283437/1‑251 (MQ=255)                                                                                                                                                                                             tataCGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCCTGTGTAGTCGACCAGAAGGAAAGGGAAGCATAGACAGGCAGTCATACTTCTCTTTGCTCTCGTCTTAGGTTACTTc                                                     >  2:290317/1‑250 (MQ=255)                                                                                                                                                                                               taCGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAANTCTCTTTGCTCTCGTCTTCGGTTACTTcta                                                   >  2:230492/1‑248 (MQ=255)                                                                                                                                                                                                  gcgATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAtgt                                               >  1:59134/1‑251 (MQ=255)                                                                                                                                                                                                    gATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                             >  1:297387/1‑251 (MQ=255)                                                                                                                                                                                                              cgtccGTCCCCGGTGCCGGGGCGGTGTTGTTTTCCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCa                                     <  1:393829/247‑1 (MQ=255)                                                                                                                                                                                                                     acacggggccataGCGGTGTTGTTTTTCACCGCAACGTCTTACGCCGGCAGACCCACCGCCTCCCAGCCGATCGGCTGCAGGACGCTTTTGCCCAGCATGCACTGGTAGCGCGCGTTCACGTCACCGCTCGTGTAGTTAAGCACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGACGCATAGACAGGCAGTACTACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGGCCGCTtcttctcctcat                              >  2:203255/13‑242 (MQ=255)                                                                                                                                                                                                                          gTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGGGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGc                         >  1:218031/1‑251 (MQ=255)                                                                                                                                                                                                                           ggCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCt                        <  1:365630/250‑1 (MQ=255)                                                                                                                                                                                                                                          ttttttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCt          <  1:230492/249‑1 (MQ=255)                                                                                                                                                                                                                                           tttttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAt        >  2:203834/1‑251 (MQ=255)                                                                                                                                                                                                                                           ttttcCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTa         <  1:80441/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cccgccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTc  <  1:61919/250‑1 (MQ=255)                                                                                                                                                                                                                                                 aCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTa                                                         >  1:394874/1‑196 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAA‑CGGT‑AGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTC‑ATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTG‑TC‑GTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674277‑674762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑