Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,450,030	A→C	P23P (CCA→CCC)	ytfQ →	galactofuranose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,450,030	0	A	C	100.0%	235.2 / NA	62	P23P (CCA→CCC)	ytfQ	galactofuranose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/31);  total (31/31)

ATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAAGAGGCGAAAGATGCCGAA  >  NC_000913/4449788‑4450273                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aTAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGt                                                                                                                                                                                                                                             >  1:48839/1‑251 (MQ=255)                  tttCATAGTGAGATGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtc                                                                                                                                                                                                                             <  1:476930/250‑1 (MQ=255)                      aTAGTGAGATGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTGTTGCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATATTCTCTGCAGTCTCGGCAGCCATGTCGTCTATTGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGtcggatc                                                                                                                                                                                                                        <  2:478547/251‑1 (MQ=255)                         gTGAGATGAAAGCGATTACAATCTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGAGTTTCGCAAGtcggatcgga                                                                                                                                                                                                                     <  1:99862/251‑1 (MQ=255)                           gagaTGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGaa                                                                                                                                                                                                                    <  2:211650/250‑1 (MQ=255)                                              aCTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGAGATGTAACGCAATCTGTTCCATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTCCCCCGCCGAGGCCAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGCCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGc                                                                                                                                                                                                   >  1:286266/1‑248 (MQ=255)                                                    gATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCa                                                                                                                                                                                           >  1:129984/1‑250 (MQ=255)                                                     aTTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGt                                                                                                                                                                                                        >  1:299120/1‑236 (MQ=255)                                                     aTTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGt                                                                                                                                                                                                        <  2:299120/236‑1 (MQ=255)                                                              ttatttaCTTTTTTGAAGTGTGATGTAACGNAATCTGTTACATAACGAATTGTCTAGAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGaa                                                                                                                                                                                 <  2:227021/250‑1 (MQ=255)                                                                      ttttttGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATc                                                                                                                                                                                                                  >  2:9819/1‑209 (MQ=255)                                                                      ttttttGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATc                                                                                                                                                                                                                  <  1:9819/209‑1 (MQ=255)                                                                       tttttGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGc                                                                                                                                                                        <  1:485304/250‑1 (MQ=255)                                                                          ttGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGa                                                                                                                                                                     <  1:325652/250‑1 (MQ=255)                                                                           tGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTAGGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGaa                                                                                                                                                                    <  2:369067/250‑1 (MQ=255)                                                                             aaGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGaaa                                                                                                                                                                   >  1:312801/1‑249 (MQ=255)                                                                                tgtgATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGa                                                                                                                                                                                                                                         >  2:88590/1‑176 (MQ=255)                                                                                tgtgATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGa                                                                                                                                                                                                                                         <  1:88590/176‑1 (MQ=255)                                                                                      gTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAAt                                                                                                                                                         <  1:464748/250‑1 (MQ=255)                                                                                       tAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATc                                                                                                                                                        >  1:26211/1‑250 (MQ=255)                                                                                       tAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACACCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGGGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGCAAAGCGCGGAATc                                                                                                                                                        >  2:89371/1‑250 (MQ=255)                                                                                        aaCGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCAc                                                                                                                                                      >  1:140660/1‑251 (MQ=255)                                                                                        aaCGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTGTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCTGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCAc                                                                                                                                                      <  1:266133/251‑1 (MQ=255)                                                                                           gCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGtt                                                                                                                                                   >  1:462749/1‑251 (MQ=255)                                                                                                   ttACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTg                                                                                                                                           >  1:120975/1‑251 (MQ=255)                                                                                                         aaCGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGAt                                                                                                                                      <  1:379281/250‑1 (MQ=255)                                                                                                             aaTTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCTGTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGTCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAATTTGCCGATGGTCa                                                                                                                                 <  2:464876/251‑1 (MQ=255)                                                                                                              aTTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTc                                                                                                                                  >  1:369312/1‑249 (MQ=255)                                                                                                              aTTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTc                                                                                                                                  >  2:358522/1‑249 (MQ=255)                                                                                                                    tataGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGAGCGGAATCACGTTGACAATTGCCGATGGTCAGCaaa                                                                                                                            >  2:337344/1‑249 (MQ=255)                                                                                                                                            tAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGc                                                                                                                                                                                    <  1:321784/169‑1 (MQ=255)                                                                                                                                            tAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGc                                                                                                                                                                                    >  2:321784/1‑169 (MQ=255)                                                                                                                                                cAATAATAACGACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGtt                                                                                              <  1:159666/251‑1 (MQ=255)                                                                                                                                                 aataatAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTc                                                                                             >  1:470581/1‑251 (MQ=255)                                                                                                                                                   taataaCTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGAGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCt                                                                                           >  1:75451/1‑251 (MQ=255)                                                                                                                                                               cgacgaGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGcaca                                                                                 >  2:352672/1‑249 (MQ=255)                                                                                                                                                                 acgaGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAggg                                                                             >  1:452175/1‑251 (MQ=255)                                                                                                                                                                  cgaGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAgggg                                                                            >  1:135361/1‑251 (MQ=255)                                                                                                                                                                    aGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGACGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTg                                                                          >  1:137634/1‑251 (MQ=255)                                                                                                                                                                          aCCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGTAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAg                                                                                                                          <  2:364130/197‑1 (MQ=255)                                                                                                                                                                          aCCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGTAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAg                                                                                                                          >  1:364130/1‑197 (MQ=255)                                                                                                                                                                          aCCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCg                                                                    >  1:126910/1‑251 (MQ=255)                                                                                                                                                                           cccTATGTGGCAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGACGCCGACAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCg                                                                    >  2:94762/1‑250 (MQ=255)                                                                                                                                                                           cccTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCg                                                                    >  1:334715/1‑250 (MQ=255)                                                                                                                                                                            ccTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGAt                                                                  >  1:412470/1‑251 (MQ=255)                                                                                                                                                                                        cGCTTACTTATAGTCTCTGCAGTCTCGGCAGCGTTGACGTCTATGGCGTTGGCCGCTCCCTTATCCGGTGTATTTTCGCAGGTCGGATCGGAATCAAGCTGGCGTGTCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTGACTTCGTTGCACAAGGGGTGTATGCGATCTTTATCGCTcc                                                      <  2:353070/251‑1 (MQ=255)                                                                                                                                                                                           ttacttaTAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCggt                                                   >  1:5390/1‑251 (MQ=255)                                                                                                                                                                                                tataGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTc                                               <  1:46672/250‑1 (MQ=255)                                                                                                                                                                                                            aGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTggg                                  <  2:183212/251‑1 (MQ=255)                                                                                                                                                                                                                 cGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGCAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGACGATGGTCAGCACAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCCAAAGGGGAGGATGCGATCTTTATCGCTCCAGTGGTCGCGACAGGTTGGGACCCg                             >  2:497776/1‑251 (MQ=255)                                                                                                                                                                                                                 cGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAAcc                              <  2:120975/250‑1 (MQ=255)                                                                                                                                                                                                                 cGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCg                             <  2:5390/251‑1 (MQ=255)                                                                                                                                                                                                                     aGCCATGTCGTCTATGGCGGTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTAt                         <  2:129984/251‑1 (MQ=255)                                                                                                                                                                                                                      gCCATGTCGTCTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCTGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTAtt                        <  2:126910/251‑1 (MQ=255)                                                                                                                                                                                                                           gtcgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAag                   <  1:209/251‑1 (MQ=255)                                                                                                                                                                                                                             cgtcTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAag                   >  1:467535/1‑249 (MQ=255)                                                                                                                                                                                                                                cTATGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATc                                                                 >  1:217911/1‑200 (MQ=255)                                                                                                                                                                                                                                cTATGGCGTGTGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGTGGTGGATGCGATc                                                                 <  2:217911/200‑1 (MQ=255)                                                                                                                                                                                                                                  aTGGCGTTGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGGCGGATCGGAAGCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACAGGTATTAAAAGAGGCGaa            <  2:412470/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCGTTGGCCGCGCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAAGAGGCGAAAGa         <  2:333061/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGGCCGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGTCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATGAAAAGAGGCGAAAGATGc      <  2:140660/250‑1 (MQ=255)                                                                                                                                                                                                                                            ccGCTCCCTTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAAGAGGCGAAAGATGCCGaa  <  1:300731/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATAACCGTAGCCGCATTTTTCATAGTGAGATGAAAGCGATTACAAACTTGTGATTAACGTTTTATTTACTTTTTTGAAGTGTGATGTAACGCAATCTGTTACATAACGAATTGTCTATAGTTTTTTCGCGAACATCTTTTAACCAATAATAACTACCCCGACGAGGACAACCCTATGTGGAAACGCTTACTTATAGTCTCTGCAGTCTCGGCAGCCATGTCGTCTATGGCGTTGGCCGCTCCATTAACCGTTGGATTTTCGCAGGTCGGATCGGAATCAGGCTGGCGTGCCGCAGAAACCAATGTGGCGAAAAGTGAAGCCGAAAAGCGCGGAATCACGTTGAAAATTGCCGATGGTCAGCAAAAGCAGGAAAACCAGATTAAAGCGGTACGTTCCTTCGTTGCACAAGGGGTGGATGCGATCTTTATCGCTCCGGTGGTCGCGACAGGTTGGGAACCGGTATTAAAAGAGGCGAAAGATGCCGAA  >  NC_000913/4449788‑4450273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑