Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,248	G→A	M111I (ATG→ATA)	rfe →	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,248	0	G	A	100.0%	172.8 / NA	48	M111I (ATG→ATA)	rfe	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/20);  total (28/20)

AAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCG  >  NC_000913/3968001‑3968488                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aaaaaGTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTataatg                                                                                                                                                                                                                                               >  2:313465/1‑251 (MQ=255)      gTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATCCCGCTCGTTGGGGGGATTTCGGTGTACGCAGGGATTTGCTTCACGTTCGGAAGTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCAGACAGGCCGCTGTTGGCATTGTTATAATGGTGtt                                                                                                                                                                                                                                          <  2:150911/251‑1 (MQ=255)             tAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCaa                                                                                                                                                                                                                                    <  1:89266/250‑1 (MQ=255)                       aCCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCa                                                                                                                                                                                                                         <  2:386123/251‑1 (MQ=255)                            aCTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGc                                                                                                                                                                                                                    <  1:282/251‑1 (MQ=255)                             cTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                                                                                                                                   >  2:97507/1‑251 (MQ=255)                                              aGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGc                                                                                                                                                                                                  >  1:402787/1‑251 (MQ=255)                                               gggATTGATTCCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCt                                                                                                                                                                                                 <  1:316025/251‑1 (MQ=255)                                               gggATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATAGTCCGCATGCATCTCTCTATCTCGCGTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCTTGGGTTATATCTGTGGCt                                                                                                                                                                                                 <  2:415137/251‑1 (MQ=255)                                                         ccTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGAt                                                                                                                                                                                       <  2:174441/251‑1 (MQ=255)                                                              cGTTGGGGGGATTTCGTCTTACGCAGGGTTTTGCTTCACGTTCGGATTTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTACATCTTTGGCTCCTGGGACATGGTGc                                                                                                                                                                                  >  2:395782/1‑251 (MQ=255)                                                               gTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGCTCGGAATTGTAGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTACCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTCTAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGcc                                                                                                                                                                                 >  2:183932/1‑250 (MQ=255)                                                                          ttCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGttt                                                                                                                                                                       <  1:313465/250‑1 (MQ=255)                                                                                       gggATTTGCTTCTCGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTTTCTCTCTTGTGGCGGTGTGCTTGTTTTCATTGGCGCGTTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGGTATAATGTTGTTCGGCAATCTTTATCTCAGTAGCCTGGTTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTCCCTGAc                                                                                                                                                         <  2:330306/251‑1 (MQ=255)                                                                                                ttCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTTATTGGCGCGCTGGATGACCGTTTTGATATAAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGTCAAGCTATATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGTGCGATGGTGCTCGGACAGTTTGGTTACTTCCTGACGCTCTTTGc                                                                                                                                                >  2:124293/1‑251 (MQ=255)                                                                                                 tCACGTTCGGAATTGTCGATTACTATATTACGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGcc                                                                                                                                               >  2:489163/1‑251 (MQ=255)                                                                                                  cACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCg                                                                                                                                              <  1:174411/251‑1 (MQ=255)                                                                                                    cGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTc                                                                                                                                            >  1:359227/1‑251 (MQ=255)                                                                                                              tGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGcc                                                                                                                                   >  1:345830/1‑250 (MQ=255)                                                                                                                 cGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCAtt                                                                                                                                >  1:265481/1‑250 (MQ=255)                                                                                                                  gATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTa                                                                                                                               >  2:469687/1‑250 (MQ=255)                                                                                                                       cTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGCCGCGTTGGATGACCGGTTTGATATCAGCGTAAAAATACGTGACACCATACAGGCCGCTGTTGGCATTGTTATAATGGAGTTCGGCAAGATTTAt                                                                                                                                                                                                                             >  1:21776/1‑151 (MQ=255)                                                                                                                       cTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGAGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCAGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGt                                                                                                                         >  1:432218/1‑251 (MQ=255)                                                                                                                       cTATATTCCGCATGCATCGCTCTATCACGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGAGCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATTCAGGCCGCTTGTGGCATTGTTATAATGGTGTTCGGCAAGCTTTAt                                                                                                                                                                                                                             <  2:21776/151‑1 (MQ=255)                                                                                                                               cGCATGCATCTCTCTATCTCGCTTGTGCCGGTTTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCCGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATg                                                                                                                 <  2:136238/251‑1 (MQ=255)                                                                                                                                gcatgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATgg                                                                                                                >  1:113644/1‑251 (MQ=255)                                                                                                                                 catgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGt                                                                                                               >  2:35359/1‑251 (MQ=255)                                                                                                                                      atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATg                                                                                                          >  1:414111/1‑251 (MQ=255)                                                                                                                                          ctctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATgg                                                                                                         <  2:272776/248‑1 (MQ=255)                                                                                                                                                 tcGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGc                                                                                               >  1:238325/1‑251 (MQ=255)                                                                                                                                                       gtgCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTCTAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTACTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGTTGGCATTGATGGCTTGCTg                                                                                         >  2:438417/1‑251 (MQ=255)                                                                                                                                                            ggTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCgg                                                                                    >  1:5103/1‑251 (MQ=255)                                                                                                                                                                 gCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGTTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGt                                                                               <  1:16803/251‑1 (MQ=255)                                                                                                                                                                        ttCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAACTCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGt                                                                        >  2:314665/1‑251 (MQ=255)                                                                                                                                                                          cATTTGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGAGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTCGCCTGGGTTATATCTTTGGCTCCTGGGAGCTGGTGCTAGGACCGTTTGGTTCCTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACTTGGTTGTTGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtct                                                                      >  2:322932/1‑251 (MQ=255)                                                                                                                                                                           aTTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGtctc                                                                     <  1:64167/251‑1 (MQ=255)                                                                                                                                                                            ttGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCg                                                                    >  1:52820/1‑251 (MQ=255)                                                                                                                                                                              ggCGCGCTGGCTGACCGTTTTGATATCAGCGTAAAAATCGGTGCCTCCATACAGGCCGCTGTTGGCATTGTTATAATGTGGTGCGGCAAGCGTTGGTTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGAGGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCAGCCACTAATGCGTTCAACATGGTTGATGGCATTGATGGCCTGCTGGGCTGGTTGTCCTGCGTCTCGtt                                                                  <  2:24497/251‑1 (MQ=255)                                                                                                                                                                                             cGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTAt                                                   <  1:469687/251‑1 (MQ=255)                                                                                                                                                                                                          gCGTAAAAATCCGAGCCACCATACAGGCCACTGATGGAATTGTTATAATGGTGTTCGGCCAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATAGCTTGCTGGGCGGGTTGTCCTGCGTCTGGTTTGCAGCAATAGGTATGATTTTGTGGTTc                                      >  2:30960/1‑251 (MQ=255)                                                                                                                                                                                                            gTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCg                                     >  1:443880/1‑250 (MQ=255)                                                                                                                                                                                                               aaaaTCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACgg                                 >  1:8613/1‑251 (MQ=255)                                                                                                                                                                                                                  aTCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGc                               >  1:482368/1‑250 (MQ=255)                                                                                                                                                                                                                    ccGTGCCACCATACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCNATCGGTATGATTTTGTGGTTCGACGGGCaa                             >  2:252272/1‑250 (MQ=255)                                                                                                                                                                                                                            ccaTACAGGCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAGACCAGCCTc                    >  1:67515/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCCGCTGTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGGTCAACATGGTTGATGGCATTGATGGCTTGCTTGGCGGATTGTCCTGCGTCTCGTTTGCAGTAATCGGGATGATTTTGNGGTTCGACGGGCAAACCAGCCTCGCCATCTg            >  2:243346/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTTGGCATTTTTATAATGGTGTTCGGCAAGGTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGc        <  2:14601/249‑1 (MQ=255)                                                                                                                                                                                                                                          gTTGGCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTCGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCtt      <  2:7011/251‑1 (MQ=255)                                                                                                                                                                                                                                              gCATTGTTATAATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTAGATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTGGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCg  <  2:423928/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 AAAAAGTCGGTTTAGTGGATAAACCAAACTTCCGCAAACGTCACCAGGGATTGATACCTCTCGTTGGGGGGATTTCGGTTTACGCAGGGATTTGCTTCACGTTCGGAATTGTCGATTACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCG  >  NC_000913/3968001‑3968488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑