Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,796	A→G	intergenic (+1239/‑405)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,796	0	A	G	100.0%	198.1 / NA	65	intergenic (+1239/‑405)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/36);  total (29/36)

AGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCA  >  NC_000913/3951564‑3952038                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   aGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAc                                                                                                                                                                                                                                  <  2:419131/251‑1 (MQ=255)     gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGAt                                                                                                                                                                                                                               <  2:260994/250‑1 (MQ=255)        tgatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATAccc                                                                                                                                                                                                                           >  1:335643/1‑250 (MQ=255)         gatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGTACAAACGATACCGt                                                                                                                                                                                                                          >  1:58967/1‑251 (MQ=255)          atgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGtc                                                                                                                                                                                                                         <  1:132561/251‑2 (MQ=255)              acatgccgggCGTTAAGACCAGCCCGGTGACTCTGGCTACGCGCCGTTGTTGTTAAACCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACTTCGCCCACACTCGCCCTGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGTTTTACCGGCTGCGGTTTGCGCGCAAGTCGCGCGACCATACGATACCGTCGTCt                                                                                                                                                                                                                     <  2:72399/243‑1 (MQ=255)                  gccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGTCTGTAt                                                                                                                                                                                                                 >  1:441348/2‑251 (MQ=255)                   ccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCAAACGATACCGGCGTCTGTAtc                                                                                                                                                                                                                >  1:336317/1‑251 (MQ=255)                         gTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGt                                                                                                                                                                                                          <  2:421578/251‑1 (MQ=255)                           tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCGCGGCACCAAACGCTACCGTAGTCCGTATCTCCggatt                                                                                                                                                                                                        >  1:468977/1‑248 (MQ=255)                              gACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACgg                                                                                                                                                                                                     >  1:24689/1‑251 (MQ=255)                                  aTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGctct                                                                                                                                                                                                 <  2:295333/251‑1 (MQ=255)                                   ccccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCtt                                                                                                                                                                                                <  2:418214/250‑1 (MQ=255)                                        gTGACGCTATCTACGCGCCGTTGTTGTTAAANCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                                                                                                                                                                           <  2:247086/251‑1 (MQ=255)                                        gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCCCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                                                                                                                                                                           >  1:136080/1‑251 (MQ=255)                                                aTCTACTCGCCGATGTTGTTAAAACAACTGTCAGATCGTAAACCTGCAGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCTCAGCACATCGCCCACACTCGCCCAGAAAATTGCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGTTTGGCGCGCAAGTCGCGCGACCAAACGATACCTTCGTCTGTATCTCCGGAGACGGCTCTTTCATGATGAAtg                                                                                                                                                                                    <  1:371769/250‑1 (MQ=255)                                                    acgcgatgttgttgttAAAACAACTGGCAGATAGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCAGCACCGGATGTGGGCGGCGCAGCACACCGCCCACACTCGCCCGGAAAATTTCAGCACCGGCAGCGGCTTAGGCACCATGGGTTTTGGTTTTCCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCa                                                                                                                                                                                <  2:357176/243‑1 (MQ=255)                                                    aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                                                                                                                                                                                  <  2:122063/248‑1 (MQ=255)                                                    aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                                                                                                                                                                                  <  1:156768/248‑1 (MQ=255)                                                     cgcgCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCACAAACGATACCGCCGTCTGTATACCCGGTGACGGCTCTTTCATGGTGAAAGTGCa                                                                                                                                                                                >  1:301321/1‑249 (MQ=255)                                                                tgttAAAACAACTGTCAGATCGTAAACCTGNGGATTGCGTCGTGACCACAGATGTGGGGAAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCAc                                                                                                                                                                    <  2:222194/250‑1 (MQ=255)                                                                   tAAAACAACTGTCAGATCGCAAACCTGCGGGTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGTGCCTCACCGCGCACACTCTCGCCCTGAAAATTTCATCACATCCAGCGGCGTAGGCCCCATGGGTTTTGGTTTACCGGCGTCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGGCGGTATCTCCGGTGACGGCGCTTTCATGATGAATGTGCAAGAGCTTGGCACCGt                                                                                                                                                                 <  2:135425/250‑1 (MQ=255)                                                                    aaaaCAACTGTCAGTGCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGTGCTGCGCAGCACAGCGCCCACCCTCGCCCGGAAAATTTCATCACCTGCAGCGGCGTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACTGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTaa                                                                                                                                                               <  2:430004/251‑1 (MQ=255)                                                                           cggtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCa                                                                                                                                                                     <  1:131231/232‑1 (MQ=255)                                                                            tGTCAGATCGTACACCTGCGGATTGCGTCGTGGCCACAATTGTGGGGCAGCCCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGACGCGCAAGTTGCGCGACCCAACAGTCTCGTCGTCTGTATTCCCGGTGACGGCTCTTTCCTGATTATTGAGCAAGAGATGAGCACAGTAAAACGccac                                                                                                                                                       >  1:442202/1‑248 (MQ=255)                                                                             gtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGAGCGCCAGTCGCGCGCCCAAACGATACCGACGTCTGTATCTCCGGTGACGGCCCTTTCTTGATGAATGTGCCAAAGCTGGGTACCGTAAAACGCAAGc                                                                                                                                                      >  2:446649/5‑251 (MQ=255)                                                                               cagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCAAACGATACCGTCGGCTGTATCTCCGGTGACGGCTCCTTCATGATGAAAGGGCAAGCGCTGGCCACCGTAAAACGCAAGc                                                                                                                                                      >  1:255525/3‑249 (MQ=255)                                                                                agATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGt                                                                                                                                                   >  1:370757/2‑251 (MQ=255)                                                                                       aaaCCTGCGGATTGCGTAGTGACCACAGATGTGGGGCAGCAACAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGGGCGCAAGTCACGCGACCAAACGATACCGTCGTCTGTATTCCCGGTGAAGGCTCTTTCATGCTGAATGTGCAAGAGCTGGGCCCCGTCAAACGCAAGCAGTTACCGtt                                                                                                                                            >  2:61944/1‑251 (MQ=255)                                                                                           cTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCCCAAACGCTACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGCAAGCCGTTACCGTTGaa                                                                                                                                         >  1:71301/1‑250 (MQ=255)                                                                                               ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCCCAAACGCTACCGTCGTCTGGATCCCCGGTGGCGGCTCTTTCCTGATGGATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGCAAAt                                                                                                                                      >  2:493854/1‑249 (MQ=255)                                                                                               ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCAtgatga                                                                                                                                                                                       <  1:32469/200‑1 (MQ=255)                                                                                               ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGGTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCg                                                                                                                                    >  1:20331/1‑251 (MQ=255)                                                                                                    gcttcgggACCACAGATTTGGGGCAGCACCNGATGTGGGCTGGGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCtt                                                                                                                                <  2:255525/243‑1 (MQ=255)                                                                                                        cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCg                                                                                                                           >  1:137714/1‑251 (MQ=255)                                                                                                        cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGNAAGCAGTTACCGTTGAAAATCggactactcg                                                                                                                           >  2:246336/1‑242 (MQ=255)                                                                                                           gACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATa                                                                                                                        <  2:137714/251‑1 (MQ=255)                                                                                                              cacaGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCCGTTACCGTTGAAAATCGTCTTACTCGATAAcc                                                                                                                     >  1:260299/1‑251 (MQ=255)                                                                                                               acaGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTAGTCTGTATCTCCGGTGACGGCTCTTTCATGAAGAATGTGCAAGAGGTGGGAACCGTAAAACGCAAGCAGTTACCGTTGAAAAACGTCTTACTCgtaaacc                                                                                                                     >  1:195914/1‑244 (MQ=255)                                                                                                                 aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTTGGCACCGGAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                                                                  >  1:148659/1‑251 (MQ=255)                                                                                                                     gtgGGGCAGCCCCAGATGTGGGCGTCGCAGCATATCGCCCACCCTCGTCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCTCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCTTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGt                                                                                                               <  2:329925/250‑1 (MQ=255)                                                                                                                        gggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTTCTCGATAACCAACGGTTAgg                                                                                                           <  1:373533/251‑1 (MQ=255)                                                                                                                         ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGATACCGTTGAAAATCGGCTTACTCGATAACCAACGGTTAggg                                                                                                          >  1:156760/1‑251 (MQ=255)                                                                                                                               aCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGtt                                                                                                    <  1:493854/251‑1 (MQ=255)                                                                                                                                        gggCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATAGTCTTACTCGCTAACCAACGGGTAGGGGTGGGTCGACAATgg                                                                                           >  2:192871/1‑251 (MQ=255)                                                                                                                                                             acacTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGGCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGtttttcccgga                                                                       >  1:255848/1‑244 (MQ=255)                                                                                                                                                               acTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCCAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGGCTTACTCGGTAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACCGtttttcccggga                                                                      >  2:13703/1‑242 (MQ=255)                                                                                                                                                                  cGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGAt                                                                  <  2:472964/250‑1 (MQ=255)                                                                                                                                                                    cccGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCACGTCGCGCGACCAAACGATTCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTGCGACAATGGCAGCAACTGTTTTTCCAGGAACGATata                                                               <  2:136080/251‑3 (MQ=255)                                                                                                                                                                         aaaaTTTCATCACCTCCGGCGGCTTAGGCACCATGGGTTTTGTTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTAGCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGaa                                                          <  2:32298/251‑1 (MQ=255)                                                                                                                                                                           aaTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAac                                                        <  2:29035/251‑1 (MQ=255)                                                                                                                                                                            aTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGGTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAacc                                                       >  1:268580/1‑251 (MQ=255)                                                                                                                                                                                           gCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACTATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGAACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAc                                        <  2:361328/251‑1 (MQ=255)                                                                                                                                                                                            cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCg                                                                                                                           >  1:100865/5‑167 (MQ=255)                                                                                                                                                                                            cggcttAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCg                                                                                                                           <  2:100865/163‑1 (MQ=255)                                                                                                                                                                                                 tAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCg                                    <  1:126782/249‑1 (MQ=255)                                                                                                                                                                                                  aggcaCCATGGGTTCTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGAt                                  <  1:253909/246‑1 (MQ=255)                                                                                                                                                                                                     caCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTcc                              <  1:13703/250‑1 (MQ=255)                                                                                                                                                                                                         aTGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACTGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCAt                          <  2:301321/251‑1 (MQ=255)                                                                                                                                                                                                           gggTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGt                        <  2:260299/251‑1 (MQ=255)                                                                                                                                                                                                                 tGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGcc                   <  1:190972/250‑1 (MQ=255)                                                                                                                                                                                                                             ggcggTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                                                                  >  1:402500/1‑143 (MQ=255)                                                                                                                                                                                                                             ggcggTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                                                                  <  2:402500/143‑1 (MQ=255)                                                                                                                                                                                                                               cggTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATc    <  1:192871/251‑1 (MQ=255)                                                                                                                                                                                                                                 gTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGCGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGGTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAACCCCGATTTCCTCATGTTAACCAGCGCCTTCGGCCTccc  >  1:2594/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   AGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAACCCCGATTTCCTCATGTTAGCCAGCGCCTTCGGCATCCA  >  NC_000913/3951564‑3952038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑