Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,754	T→C	intergenic (+1197/‑447)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,754	0	T	C	100.0%	244.0 / NA	72	intergenic (+1197/‑447)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/36);  total (36/36)

TTACCAGCATTACAGCAGCCGTT‑AA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAAC  >  NC_000913/3951511‑3952000                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       ttACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAgg                                                                                                                                                                                                                                                   >  1:150636/1‑251 (MQ=255)  gaCCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGTTGTGGGCTGCGCAGCACATAGCCCACACTCGCCCGGAAAATTTCGTCACATCCAGCGGCTTAggc                                                                                                                                                                                                                                                  >  2:398915/2‑250 (MQ=255)   aCCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                                                                                                                                  >  1:242378/1‑249 (MQ=255)    ccAGCATTACAGCAGCCGTTCAAT‑AT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggcac                                                                                                                                                                                                                                                >  1:122063/1‑248 (MQ=255)      aGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCa                                                                                                                                                                                                                                              <  2:494884/251‑1 (MQ=255)             cggcagcCGTT‑AA‑‑ATAACAATGACTTGCAGCNACACTGCGCGCAGCTGCGGGATGAACATGCCTGGGGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTGAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGttt                                                                                                                                                                                                                                       <  2:250146/249‑1 (MQ=255)             cagcagCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGttt                                                                                                                                                                                                                                       <  1:94571/251‑1 (MQ=255)              agcagcCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGtttt                                                                                                                                                                                                                                      >  1:472964/1‑251 (MQ=255)              agcagcCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGtttt                                                                                                                                                                                                                                      >  1:351902/1‑251 (MQ=255)                  gcCGTT‑AAATAT‑‑CAATGACTGGCCGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACAATCCCGGTGACGCTATCTACGCGCCGTTGCTGTTACAACAACTGACAGATCGTAAACATGCGGCTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGACCACACTCGCCCGGAAACTTTCATCACCTCCAGCGGCTTCGGCACCATGGGTTTTGGtt                                                                                                                                                                                                                                  >  2:201802/1‑251 (MQ=255)                     cgttaaataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTa                                                                                                                                                                                                                                <  1:142994/243‑1 (MQ=255)                          aataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggc                                                                                                                                                                                                                           >  1:138754/4‑251 (MQ=255)                           ataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcg                                                                                                                                                                                                                          <  1:286886/249‑1 (MQ=255)                                         ggCAGCTACACTGCGCGCAGCTGCGTGATGNACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcgc                                                                                                                                                                                                               <  2:244730/250‑1 (MQ=255)                                         ggCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcg                                                                                                                                                                                                                <  2:134102/249‑1 (MQ=255)                                                           aGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAc                                                                                                                                                                                            <  2:419131/251‑1 (MQ=255)                                                               gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGAt                                                                                                                                                                                         <  2:260994/250‑1 (MQ=255)                                                                  tgatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATAccc                                                                                                                                                                                     >  1:335643/1‑250 (MQ=255)                                                                   gatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGTACAAACGATACCGt                                                                                                                                                                                    >  1:58967/1‑251 (MQ=255)                                                                    atgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGtc                                                                                                                                                                                   <  1:132561/251‑2 (MQ=255)                                                                        acatgccgggCGTTAAGACCAGCCCGGTGACTCTGGCTACGCGCCGTTGTTGTTAAACCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACTTCGCCCACACTCGCCCTGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGTTTTACCGGCTGCGGTTTGCGCGCAAGTCGCGCGACCATACGATACCGTCGTCt                                                                                                                                                                               <  2:72399/243‑1 (MQ=255)                                                                            gccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGTCTGTAt                                                                                                                                                                           >  1:441348/2‑251 (MQ=255)                                                                             ccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCAAACGATACCGGCGTCTGTAtc                                                                                                                                                                          >  1:336317/1‑251 (MQ=255)                                                                                   gTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGt                                                                                                                                                                    <  2:421578/251‑1 (MQ=255)                                                                                     tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCGCGGCACCAAACGCTACCGTAGTCCGTATCTCCggatt                                                                                                                                                                  >  1:468977/1‑248 (MQ=255)                                                                                        gACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACgg                                                                                                                                                               >  1:24689/1‑251 (MQ=255)                                                                                          ccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                                                                                                                                  >  2:5333/1‑165 (MQ=255)                                                                                          ccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                                                                                                                                  <  1:5333/166‑2 (MQ=255)                                                                                            aTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGctct                                                                                                                                                           <  2:295333/251‑1 (MQ=255)                                                                                             ccccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCtt                                                                                                                                                          <  2:418214/250‑1 (MQ=255)                                                                                                  gTGACGCTATCTACGCGCCGTTGTTGTTAAANCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                                                                                                                                     <  2:247086/251‑1 (MQ=255)                                                                                                  gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCCCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                                                                                                                                     >  1:136080/1‑251 (MQ=255)                                                                                                          aTCTACTCGCCGATGTTGTTAAAACAACTGTCAGATCGTAAACCTGCAGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCTCAGCACATCGCCCACACTCGCCCAGAAAATTGCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGTTTGGCGCGCAAGTCGCGCGACCAAACGATACCTTCGTCTGTATCTCCGGAGACGGCTCTTTCATGATGAAtg                                                                                                                                              <  1:371769/250‑1 (MQ=255)                                                                                                              acgcgatgttgttgttAAAACAACTGGCAGATAGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCAGCACCGGATGTGGGCGGCGCAGCACACCGCCCACACTCGCCCGGAAAATTTCAGCACCGGCAGCGGCTTAGGCACCATGGGTTTTGGTTTTCCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCa                                                                                                                                          <  2:357176/243‑1 (MQ=255)                                                                                                              aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                                                                                                                                            <  1:156768/248‑1 (MQ=255)                                                                                                              aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                                                                                                                                            <  2:122063/248‑1 (MQ=255)                                                                                                               cgcgCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCACAAACGATACCGCCGTCTGTATACCCGGTGACGGCTCTTTCATGGTGAAAGTGCa                                                                                                                                          >  1:301321/1‑249 (MQ=255)                                                                                                                          tgttAAAACAACTGTCAGATCGTAAACCTGNGGATTGCGTCGTGACCACAGATGTGGGGAAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCAc                                                                                                                              <  2:222194/250‑1 (MQ=255)                                                                                                                             tAAAACAACTGTCAGATCGCAAACCTGCGGGTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGTGCCTCACCGCGCACACTCTCGCCCTGAAAATTTCATCACATCCAGCGGCGTAGGCCCCATGGGTTTTGGTTTACCGGCGTCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGGCGGTATCTCCGGTGACGGCGCTTTCATGATGAATGTGCAAGAGCTTGGCACCGt                                                                                                                           <  2:135425/250‑1 (MQ=255)                                                                                                                              aaaaCAACTGTCAGTGCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGTGCTGCGCAGCACAGCGCCCACCCTCGCCCGGAAAATTTCATCACCTGCAGCGGCGTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACTGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTaa                                                                                                                         <  2:430004/251‑1 (MQ=255)                                                                                                                                     cggtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCa                                                                                                                               <  1:131231/232‑1 (MQ=255)                                                                                                                                      tGTCAGATCGTACACCTGCGGATTGCGTCGTGGCCACAATTGTGGGGCAGCCCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGACGCGCAAGTTGCGCGACCCAACAGTCTCGTCGTCTGTATTCCCGGTGACGGCTCTTTCCTGATTATTGAGCAAGAGATGAGCACAGTAAAACGccac                                                                                                                 >  1:442202/1‑248 (MQ=255)                                                                                                                                       gtcagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGAGCGCCAGTCGCGCGCCCAAACGATACCGACGTCTGTATCTCCGGTGACGGCCCTTTCTTGATGAATGTGCCAAAGCTGGGTACCGTAAAACGCAAGc                                                                                                                >  2:446649/5‑251 (MQ=255)                                                                                                                                         cagATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCAAACGATACCGTCGGCTGTATCTCCGGTGACGGCTCCTTCATGATGAAAGGGCAAGCGCTGGCCACCGTAAAACGCAAGc                                                                                                                >  1:255525/3‑249 (MQ=255)                                                                                                                                          agATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGt                                                                                                             >  1:370757/2‑251 (MQ=255)                                                                                                                                                tAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTg                                                                                                                                                                                                                                     >  1:446766/1‑125 (MQ=255)                                                                                                                                                tAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTg                                                                                                                                                                                                                                     <  2:446766/125‑1 (MQ=255)                                                                                                                                                 aaaCCTGCGGATTGCGTAGTGACCACAGATGTGGGGCAGCAACAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGGGCGCAAGTCACGCGACCAAACGATACCGTCGTCTGTATTCCCGGTGAAGGCTCTTTCATGCTGAATGTGCAAGAGCTGGGCCCCGTCAAACGCAAGCAGTTACCGtt                                                                                                      >  2:61944/1‑251 (MQ=255)                                                                                                                                                     cTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCCCAAACGCTACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGGAAAACGCAAGCCGTTACCGTTGaa                                                                                                   >  1:71301/1‑250 (MQ=255)                                                                                                                                                         ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCCCAAACGCTACCGTCGTCTGGATCCCCGGTGGCGGCTCTTTCCTGATGGATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCGGTTACCGTTGCAAAt                                                                                                >  2:493854/1‑249 (MQ=255)                                                                                                                                                         ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCAtgatga                                                                                                                                                 <  1:32469/200‑1 (MQ=255)                                                                                                                                                         ggATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGGTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCg                                                                                              >  1:20331/1‑251 (MQ=255)                                                                                                                                                              gcttcgggACCACAGATTTGGGGCAGCACCNGATGTGGGCTGGGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCtt                                                                                          <  2:255525/243‑1 (MQ=255)                                                                                                                                                                  cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCg                                                                                     >  1:137714/1‑251 (MQ=255)                                                                                                                                                                  cgtGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGNAAGCAGTTACCGTTGAAAATCggactactcg                                                                                     >  2:246336/1‑242 (MQ=255)                                                                                                                                                                     gACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATa                                                                                  <  2:137714/251‑1 (MQ=255)                                                                                                                                                                        cacaGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCCGTTACCGTTGAAAATCGTCTTACTCGATAAcc                                                                               >  1:260299/1‑251 (MQ=255)                                                                                                                                                                         acaGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTAGTCTGTATCTCCGGTGACGGCTCTTTCATGAAGAATGTGCAAGAGGTGGGAACCGTAAAACGCAAGCAGTTACCGTTGAAAAACGTCTTACTCgtaaacc                                                                               >  1:195914/1‑244 (MQ=255)                                                                                                                                                                           aGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTTGGCACCGGAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAAc                                                                            >  1:148659/1‑251 (MQ=255)                                                                                                                                                                               gtgGGGCAGCCCCAGATGTGGGCGTCGCAGCATATCGCCCACCCTCGTCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCTCCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCTTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGt                                                                         <  2:329925/250‑1 (MQ=255)                                                                                                                                                                                  gggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTTCTCGATAACCAACGGTTAgg                                                                     <  1:373533/251‑1 (MQ=255)                                                                                                                                                                                   ggCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGATACCGTTGAAAATCGGCTTACTCGATAACCAACGGTTAggg                                                                    >  1:156760/1‑251 (MQ=255)                                                                                                                                                                                         aCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGtt                                                              <  1:493854/251‑1 (MQ=255)                                                                                                                                                                                                  gggCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCCCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATAGTCTTACTCGCTAACCAACGGGTAGGGGTGGGTCGACAATgg                                                     >  2:192871/1‑251 (MQ=255)                                                                                                                                                                                                                       acacTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGGCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGGTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGtttttcccgga                                 >  1:255848/1‑244 (MQ=255)                                                                                                                                                                                                                         acTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCCAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGGCTTACTCGGTAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACCGtttttcccggga                                >  2:13703/1‑242 (MQ=255)                                                                                                                                                                                                                            cGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGAt                            <  2:472964/250‑1 (MQ=255)                                                                                                                                                                                                                              cccGGAAAATTTCATCCCCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCACGTCGCGCGACCAAACGATTCCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTGCGACAATGGCAGCAACTGTTTTTCCAGGAACGATata                         <  2:136080/251‑3 (MQ=255)                                                                                                                                                                                                                                   aaaaTTTCATCACCTCCGGCGGCTTAGGCACCATGGGTTTTGTTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTAGCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGaa                    <  2:32298/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAac                  <  2:29035/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGGGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGGTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAacc                 >  1:268580/1‑251 (MQ=255)                                                                                                                                                                                                                                                     gCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACTATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGAACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTCCAGGAACGATATAGCGAAACCACCCTTACCGATAAc  <  2:361328/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       TTACCAGCATTACAGCAGCCGTT‑AA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAGCAGTTACCGTTGAAAATCGTCTTACTCGATAACCAACGGTTAGGGATGGTTCGACAATGGCAGCAACTGTTTTTTCAGGAACGATACAGCGAAACCACCCTTACTGATAAC  >  NC_000913/3951511‑3952000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑