Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,643	G→A	intergenic (+1086/‑558)	ilvX → / → ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,643	0	G	A	100.0%	276.7 / NA	75	intergenic (+1086/‑558)	ilvX/ilvM	uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/38);  total (37/38)

CACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAG  >  NC_000913/3951405‑3951889                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cacCTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAAc                                                                                                                                                                                                                                              <  2:147020/251‑1 (MQ=255)   ccTTCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAAGGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACGACTGTCATATCGTAAACCt                                                                                                                                                                                                                                            >  1:222194/1‑251 (MQ=255)      tCGCACCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTg                                                                                                                                                                                                                                           <  1:169273/249‑1 (MQ=255)         caccACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGAt                                                                                                                                                                                                                                      >  1:181879/3‑251 (MQ=255)          cccACACGCCAGTGTTATCCAGATGGATATCGACCCGGCAGAAATGAACAAGCGGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGAtt                                                                                                                                                                                                                                     <  2:155581/250‑1 (MQ=255)              cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCTGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACGGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAGCTGTCAGATCGTAAACCTGCGGATTGcgt                                                                                                                                                                                                                                 <  1:423258/251‑1 (MQ=255)                cGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACAGGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACGGTCAGATCGTAAACCTGCGGATTGcgtcg                                                                                                                                                                                                                               <  1:418253/251‑1 (MQ=255)                   catggtTATCCATATTGCTATCGACCCGGCAGAAATGAACAAGCTGCGTCAGTCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTA‑AAATAT‑‑CAATGACTGGCAGCTACACTGCTCGCAGCTGCGTGATGAACATGCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCCGATTTCGTCGTGa                                                                                                                                                                                                                            <  2:165429/247‑1 (MQ=255)                       gtTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACcac                                                                                                                                                                                                                        >  1:24147/1‑251 (MQ=255)                                atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGcgtc                                                                                                                                                                                                                                <  1:436697/234‑1 (MQ=255)                                atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGcgtc                                                                                                                                                                                                                                >  2:436697/1‑234 (MQ=255)                                  ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                                                                                                                                                                                             >  1:131121/1‑251 (MQ=255)                                  ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCATAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                                                                                                                                                                                             >  1:31656/1‑251 (MQ=255)                                        cGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAcc                                                                                                                                                                                                       <  1:407735/251‑1 (MQ=255)                                                     aTGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgc                                                                                                                                                                                          >  1:418214/1‑251 (MQ=255)                                                           aaGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCGGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgcagca                                                                                                                                                                                    <  1:372742/251‑1 (MQ=255)                                                                gCGTCGGGCACATGTGGCATTACAAGGTGATNTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCg                                                                                                                                                                               <  2:254589/251‑1 (MQ=255)                                                                        cacaTGTGGCATTACACGTTGATTTAAAGGCTCTGTTACCAGCCTTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGTCAGCTACACGGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACt                                                                                                                                                                       <  2:481077/251‑1 (MQ=255)                                                                        cacaTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGATACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACCACCATCCCGGTGAAGCTATCTACGCGCCTTTGTTGTTAAAACAACTGACAGATCGTAAACCTGAGGACTGCGTCGTGTCCACAGATGTGGGGCAGCACCAGATGTGGTCTGCGCATCACATCGCCCACACt                                                                                                                                                                       >  2:129425/1‑251 (MQ=255)                                                                           aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGc                                                                                                                                                                    >  1:263431/1‑251 (MQ=255)                                                                            tgtgGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCATGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGcc                                                                                                                                                                   >  2:154136/1‑251 (MQ=255)                                                                             gtgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGccc                                                                                                                                                                  <  2:41316/251‑1 (MQ=255)                                                                              tgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCg                                                                                                                                                                 >  1:29035/1‑251 (MQ=255)                                                                               ggCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCgg                                                                                                                                                                <  2:470933/251‑1 (MQ=255)                                                                               ggCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCgg                                                                                                                                                                >  2:75839/1‑251 (MQ=255)                                                                                  aTTACAAGGTGATTTAAATGCTCTGTTACCAGCCTTACAGCAGCCGTT‑AAATAT‑‑CAATGACTTGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGAAGCTATCTACGCGCCCTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGGTGGGGGGGCGCACCAGATGTGGTCTGCGCAGCACATCGCCCACACTCGCCCGGaa                                                                                                                                                              >  1:271900/1‑250 (MQ=255)                                                                                  aTTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGaa                                                                                                                                                              >  1:361328/1‑250 (MQ=255)                                                                                    tACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAAt                                                                                                                                                           >  1:419131/1‑251 (MQ=255)                                                                                          gTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcatc                                                                                                                                                     >  1:338393/1‑251 (MQ=255)                                                                                                  aaTGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACGGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAg                                                                                                                                             <  2:338393/251‑1 (MQ=255)                                                                                                   aTGCTCTGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGGTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACATGCGGCTAGCGTCGGGACCACAGATGTGGGGCAGCACCAGAGGTGGGCGGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcatcaaaccc                                                                                                                                               >  2:373533/1‑243 (MQ=255)                                                                                                        ctGTTACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTACAGCgtctt                                                                                                                                       >  2:102911/1‑247 (MQ=255)                                                                                                           ttACCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAgg                                                                                                                                    >  1:150636/1‑251 (MQ=255)                                                                                                            gaCCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGTTGTGGGCTGCGCAGCACATAGCCCACACTCGCCCGGAAAATTTCGTCACATCCAGCGGCTTAggc                                                                                                                                   >  2:398915/2‑250 (MQ=255)                                                                                                             aCCAGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                   >  1:242378/1‑249 (MQ=255)                                                                                                              ccAGCATTACAGCAGCCGTTCAAT‑AT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggcac                                                                                                                                 >  1:122063/1‑248 (MQ=255)                                                                                                                aGCATTACAGCAGCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCa                                                                                                                               <  2:494884/251‑1 (MQ=255)                                                                                                                       cggcagcCGTT‑AA‑‑ATAACAATGACTTGCAGCNACACTGCGCGCAGCTGCGGGATGAACATGCCTGGGGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTGAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGttt                                                                                                                        <  2:250146/249‑1 (MQ=255)                                                                                                                       cagcagCCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGttt                                                                                                                        <  1:94571/251‑1 (MQ=255)                                                                                                                        agcagcCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGtttt                                                                                                                       >  1:351902/1‑251 (MQ=255)                                                                                                                        agcagcCGTT‑AAATAT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGtttt                                                                                                                       >  1:472964/1‑251 (MQ=255)                                                                                                                            gcCGTT‑AAATAT‑‑CAATGACTGGCCGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACAATCCCGGTGACGCTATCTACGCGCCGTTGCTGTTACAACAACTGACAGATCGTAAACATGCGGCTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGACCACACTCGCCCGGAAACTTTCATCACCTCCAGCGGCTTCGGCACCATGGGTTTTGGtt                                                                                                                   >  2:201802/1‑251 (MQ=255)                                                                                                                               cgttaaataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTa                                                                                                                 <  1:142994/243‑1 (MQ=255)                                                                                                                                    aataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggc                                                                                                            >  1:138754/4‑251 (MQ=255)                                                                                                                                     ataT‑‑CAATGACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACcggcg                                                                                                           <  1:286886/249‑1 (MQ=255)                                                                                                                                               gACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtgtg                                                                                                                                                                                                >  2:339289/1‑158 (MQ=255)                                                                                                                                               gACTGGCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtgtg                                                                                                                                                                                                <  1:339289/158‑1 (MQ=255)                                                                                                                                                   ggCAGCTACACTGCGCGCAGCTGCGTGATGNACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcgc                                                                                                <  2:244730/250‑1 (MQ=255)                                                                                                                                                   ggCAGCTACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcg                                                                                                 <  2:134102/249‑1 (MQ=255)                                                                                                                                                                     aGCTGCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAAc                                                                             <  2:419131/251‑1 (MQ=255)                                                                                                                                                                         gCGTGATGAACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGAt                                                                          <  2:260994/250‑1 (MQ=255)                                                                                                                                                                            tgatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATAccc                                                                      >  1:335643/1‑250 (MQ=255)                                                                                                                                                                             gatgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGTACAAACGATACCGt                                                                     >  1:58967/1‑251 (MQ=255)                                                                                                                                                                              atgaACATGCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGtc                                                                    <  1:132561/251‑2 (MQ=255)                                                                                                                                                                                  acatgccgggCGTTAAGACCAGCCCGGTGACTCTGGCTACGCGCCGTTGTTGTTAAACCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACTTCGCCCACACTCGCCCTGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGTTTTACCGGCTGCGGTTTGCGCGCAAGTCGCGCGACCATACGATACCGTCGTCt                                                                <  2:72399/243‑1 (MQ=255)                                                                                                                                                                                      gccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGTCTGTAt                                                            >  1:441348/2‑251 (MQ=255)                                                                                                                                                                                       ccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCCAGTCGCGCGACCAAACGATACCGGCGTCTGTAtc                                                           >  1:336317/1‑251 (MQ=255)                                                                                                                                                                                             gTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGt                                                     <  2:421578/251‑1 (MQ=255)                                                                                                                                                                                               tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCGCAAGTCGCGGCACCAAACGCTACCGTAGTCCGTATCTCCggatt                                                   >  1:468977/1‑248 (MQ=255)                                                                                                                                                                                                  gACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACgg                                                >  1:24689/1‑251 (MQ=255)                                                                                                                                                                                                    ccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                   >  2:5333/1‑165 (MQ=255)                                                                                                                                                                                                    ccATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAggc                                                                                                                                   <  1:5333/166‑2 (MQ=255)                                                                                                                                                                                                      aTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGctct                                            <  2:295333/251‑1 (MQ=255)                                                                                                                                                                                                       ccccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCtt                                           <  2:418214/250‑1 (MQ=255)                                                                                                                                                                                                            gTGACGCTATCTACGCGCCGTTGTTGTTAAANCAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                      <  2:247086/251‑1 (MQ=255)                                                                                                                                                                                                            gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCCCGACCAAACGATACCGGCGTCTGTATCTCCGGTGACGGCTCTTTCatg                                      >  1:136080/1‑251 (MQ=255)                                                                                                                                                                                                                    aTCTACTCGCCGATGTTGTTAAAACAACTGTCAGATCGTAAACCTGCAGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCTCAGCACATCGCCCACACTCGCCCAGAAAATTGCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGTTTGGCGCGCAAGTCGCGCGACCAAACGATACCTTCGTCTGTATCTCCGGAGACGGCTCTTTCATGATGAAtg                               <  1:371769/250‑1 (MQ=255)                                                                                                                                                                                                                        acgcgatgttgttgttAAAACAACTGGCAGATAGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCAGCACCGGATGTGGGCGGCGCAGCACACCGCCCACACTCGCCCGGAAAATTTCAGCACCGGCAGCGGCTTAGGCACCATGGGTTTTGGTTTTCCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTACGGTGACGGCTCTTTCATGATGAATGTGCa                           <  2:357176/243‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                             <  1:156768/248‑1 (MQ=255)                                                                                                                                                                                                                        aCGCGCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAAtgtg                             <  2:122063/248‑1 (MQ=255)                                                                                                                                                                                                                         cgcgCCGTTGTTGTTAAAACAACTGTCAGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGCACAAACGATACCGCCGTCTGTATACCCGGTGACGGCTCTTTCATGGTGAAAGTGCa                           >  1:301321/1‑249 (MQ=255)                                                                                                                                                                                                                                    tgttAAAACAACTGTCAGATCGTAAACCTGNGGATTGCGTCGTGACCACAGATGTGGGGAAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCAGGGGTTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCAc               <  2:222194/250‑1 (MQ=255)                                                                                                                                                                                                                                       tAAAACAACTGTCAGATCGCAAACCTGCGGGTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGTGCCTCACCGCGCACACTCTCGCCCTGAAAATTTCATCACATCCAGCGGCGTAGGCCCCATGGGTTTTGGTTTACCGGCGTCGGTTGGCGCGCAAGGCGCGCGACCAAACGATACCGTCGGCGGTATCTCCGGTGACGGCGCTTTCATGATGAATGTGCAAGAGCTTGGCACCGt            <  2:135425/250‑1 (MQ=255)                                                                                                                                                                                                                                        aaaaCAACTGTCAGTGCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGTGCTGCGCAGCACAGCGCCCACCCTCGCCCGGAAAATTTCATCACCTGCAGCGGCGTAGGCACCATGGGGTTTGGTTTACCGGCGGCGGTTGGCGCGCAAGTCGCGCGACCAAACGATACCGTCGTCTGTATCTCCGGTGACTGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTaa          <  2:430004/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGTCAGATCGTACACCTGCGGATTGCGTCGTGGCCACAATTGTGGGGCAGCCCCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGCACCATGGGTTTTGGTTTACCGGCGGCGGTTGACGCGCAAGTTGCGCGACCCAACAGTCTCGTCGTCTGTATTCCCGGTGACGGCTCTTTCCTGATTATTGAGCAAGAGATGAGCACAGTAAAACGccac  >  1:442202/1‑248 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTT‑AA‑‑AT‑‑CAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCTGTATCTCCGGTGACGGCTCTTTCATGATGAATGTGCAAGAGCTGGGCACCGTAAAACGCAAG  >  NC_000913/3951405‑3951889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑