Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,634	A→G	D45D (GAT→GAC)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,634	0	A	G	100.0%	211.9 / NA	61	D45D (GAT→GAC)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/32);  total (29/32)

ACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT  >  NC_000913/3815401‑3815878                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                                                                                                                                                       <  1:168885/249‑1 (MQ=255)       aTTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCGTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTGCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGccc                                                                                                                                                                                                                               <  2:286113/251‑1 (MQ=255)         tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATACCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATTCCCGAATCCCCCCATGCTTCCACG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCCGGATTAAACAGCCCgg                                                                                                                                                                                                                             >  1:440958/1‑251 (MQ=255)              gTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                                                                                                                                                                        <  1:347966/251‑1 (MQ=255)                   tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaaga                                                                                                                                                                                                                    <  1:365302/250‑1 (MQ=255)                           gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGAGCCACGGCGGG‑AGGGGGCGGAATCCGGGTCCCTTTGTAACGC‑GGGGCAAAAAGCAGATCGGACTCA‑ATGCCCGAATCCAACAATGCTTCAGCG‑GAAAAACGGCCCAAAAGGGCCAGGGGGCGCCCGGGGTTAAAAAGCCCGGCGTTGAAAAAATAggggg                                                                                                                                                                                                           >  1:426287/1‑250 (MQ=255)                             tACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGGGGTAGCCCTCGGGATACCTTTTTAA‑GCCGGGCCCAACACCAGATCGAA‑ACACATGCCAGAATCCACCCACGCTTCAACG‑TAAAAAACGCCTAACAGTGACACGGCGCGCCCGGTATTAAACAACCCCGCGTTTAAGAAAAAgggggct                                                                                                                                                                                                         >  2:260458/1‑248 (MQ=255)                                      gATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                                                                                                                                                                <  1:232273/251‑1 (MQ=255)                                       aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGGCGCCATCGGGATCCCCTTGTAA‑GCAGGGCCAAACAGCAGATCGAACCCC‑ATGCCCAAATCCACCAATGCTTCAGCG‑TAAAAAAGGGCTAACAGAGCCCGGGCGCGCCCGGGATTAAAAAACCCGGCGTTGAAAAAAAAGGGGGTTTTGGGCCCgg                                                                                                                                                                                               >  2:331783/1‑251 (MQ=255)                                             gccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGT‑AGTGGTGGCACACCGGATCCCTTTTTAA‑CCCGGGCCCAACAGCCGATCCAACCCC‑ATGCCCGAATCCACCCATGCTTCACCGGAAAAAACGCCCTA‑CAGTGCCCGGGCACCCCCGGGATTACACAACCCGGCGTTGGAGAAAAAAGGGCCTTTGCGCCCGGGttttt                                                                                                                                                                                         >  2:392618/1‑249 (MQ=255)                                                 tCACCGTGGTCTTTTGACTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGACACGGCGGT‑AGGGGTCGCAAAAGGGGTCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATACACCAATGCTTCAGCG‑TAAAAACGGCCTAAAAGTGCCAGGGCGCCCCCGGCATTAAACAGCCCCGCGTTGAAGAAATAAGGGCTTTTGCGCCCGGATTTCAGCgg                                                                                                                                                                                     >  1:89320/1‑250 (MQ=255)                                                             tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                                                                                                                                           <  1:314314/249‑1 (MQ=255)                                                                        gCGGTTCAAGCAGTCCGGCAGGTCAAGGTAATGATGCGCCGCCAGCGCCGCGGCGGT‑AGTGGTCGCAAGCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCCCCAATGCTTCAGCG‑TAAAAACGGCCTAACATTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGTCAAACTTTAACCCCt                                                                                                                                                              <  2:266723/251‑1 (MQ=255)                                                                        gCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGTCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAcc                                                                                                                                                               <  1:438345/250‑1 (MQ=255)                                                                                cccAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCtgcttgct                                                                                                                                                       <  2:415283/248‑1 (MQ=255)                                                                                aGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa                                                                                                                                                      <  1:331783/251‑1 (MQ=255)                                                                                        ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                                                                                                                                               >  1:486948/1‑250 (MQ=255)                                                                                          caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCCGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                                                                                                                                            >  2:413284/1‑251 (MQ=255)                                                                                                aaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAAAGGCCTAACAGTGCCAGGTCACGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCGTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTCAACACCTGCTTGCTAAGCGCAAATTCAATa                                                                                                                                       >  2:406507/2‑250 (MQ=255)                                                                                                              ccGCCAGCGCCACGGCGGTATG‑GGTCGCAATCGGGATCCCGTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATGTCAGCGTAAACTCGCGAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGat                                                                                                                          <  2:435929/249‑1 (MQ=255)                                                                                                              ccGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATCCCTATGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ACCCCAGAAGCCCCCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCTGATTTCAGCGTAAACTCGCCAAACTTGAACACCTGCTTGCTGAGCGCAAATTCAATAAACTTGCGCTGat                                                                                                                          <  2:21061/249‑1 (MQ=255)                                                                                                                   agcgCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGttt                                                                                                                   >  1:495371/4‑251 (MQ=255)                                                                                                                           ggCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACt                                                                                                                                                                       <  2:286010/190‑1 (MQ=255)                                                                                                                           ggCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACt                                                                                                                                                                       >  1:286010/2‑191 (MQ=255)                                                                                                                                        cgCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                                                                                                                                            >  2:326953/2‑206 (MQ=255)                                                                                                                                        cgCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                                                                                                                                            <  1:326953/205‑1 (MQ=255)                                                                                                                                        cgCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATc                                                                                                <  1:190797/249‑1 (MQ=255)                                                                                                                                        cgCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATc                                                                                                <  1:111471/249‑1 (MQ=255)                                                                                                                                         gCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCtt                                                                                              <  1:219928/251‑1 (MQ=255)                                                                                                                                              cGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACtt                                                                                          <  1:392618/250‑1 (MQ=255)                                                                                                                                              cGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACtt                                                                                          <  2:378564/250‑1 (MQ=255)                                                                                                                                               gggATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACATCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACtttt                                                                                        >  1:347002/1‑251 (MQ=255)                                                                                                                                                 gATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCTGAATCCACCAGTTCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGcc                                                                                                                                                                                                                                <  1:473680/113‑1 (MQ=255)                                                                                                                                                 gATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCt                                                                                      <  1:413284/251‑1 (MQ=255)                                                                                                                                                        ttGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAc                                                                                >  1:478871/1‑250 (MQ=255)                                                                                                                                                                 aGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTGCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACGGGCTCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg                                                                       <  1:414834/251‑1 (MQ=255)                                                                                                                                                                         aCAGCAGATCTAACGCA‑ATGCCAGAATCCACNAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtca                                                               <  2:244264/251‑1 (MQ=255)                                                                                                                                                                               gATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCATCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTc                                                         >  1:251182/1‑251 (MQ=255)                                                                                                                                                                                  cGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGcc                                                      <  2:440958/251‑1 (MQ=255)                                                                                                                                                                                    aaCTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCtt                                                    >  1:83844/1‑251 (MQ=255)                                                                                                                                                                                         a‑aTGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaa                                                >  1:389351/1‑250 (MQ=255)                                                                                                                                                                                               cagAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCCTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGt                                          >  2:59464/3‑251 (MQ=255)                                                                                                                                                                                                  aaTCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTc                                                                                                                                                                                          <  1:138623/104‑1 (MQ=255)                                                                                                                                                                                                  aaTCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTc                                                                                                                                                                                          >  2:138623/1‑104 (MQ=255)                                                                                                                                                                                                  aaTCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTg                                       >  1:348936/1‑251 (MQ=255)                                                                                                                                                                                                   aTCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGc                                      >  1:269474/1‑251 (MQ=255)                                                                                                                                                                                                          aatgCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTCGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                               >  1:201895/4‑251 (MQ=255)                                                                                                                                                                                                           atgCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAACAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccg                              >  1:157601/3‑251 (MQ=255)                                                                                                                                                                                                              cTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCt                           >  2:341428/1‑251 (MQ=255)                                                                                                                                                                                                                  aGCG‑TAAAAACGGCCTAACATGGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                       <  1:97026/251‑1 (MQ=255)                                                                                                                                                                                                                  aGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGATGAAGAAATAGGGGCTTTTGAGCCCGGATTTCAGCGTAAACTCGACAAACTTTAACCCCTGCTTGCTAAGCGCAAATTCAATAAACTGACGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTAAAGCCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTAGCCAGCGCAGCCTTCtc                       >  2:123388/1‑250 (MQ=255)                                                                                                                                                                                                                   gCG‑TAAAAACGGCCTAACACTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGc                      <  1:237779/251‑1 (MQ=255)                                                                                                                                                                                                                        aaaaaCGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAANAATCAGTTTGCCAGCGCCGCCTTCTGCGTCg                  >  2:238538/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaaaCGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTc                   <  1:275712/250‑1 (MQ=255)                                                                                                                                                                                                                               gCCTAACAGTGCCAGGTCGCGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGNTTGCCAGCGCCGCCTTCTGCGCCGCTACAAt           >  2:217938/1‑251 (MQ=255)                                                                                                                                                                                                                                    aCAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtcatc                                                             <  2:237557/196‑1 (MQ=255)                                                                                                                                                                                                                                    aCAGTGCCAGGTCGCGCCCGGGATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAGAAAAAGGCGACtcatc                                                             >  1:237557/1‑196 (MQ=255)                                                                                                                                                                                                                                       gTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga   <  2:296454/251‑1 (MQ=255)                                                                                                                                                                                                                                        tGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTg                                                                                                                                  >  1:71826/1‑123 (MQ=255)                                                                                                                                                                                                                                        tGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTg                                                                                                                                  <  2:71826/123‑1 (MQ=255)                                                                                                                                                                                                                                         gCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat  <  2:89320/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     ACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAT  >  NC_000913/3815401‑3815878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑