Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,523	A→G	A82A (GCT→GCC)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,523	0	A	G	100.0%	128.7 / NA	42	A82A (GCT→GCC)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/30);  total (12/30)

TCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTT  >  NC_000913/3815281‑3815765                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ccgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtagt                                                                                                                                                                                                                                                <  1:454557/250‑4 (MQ=255)         cccccgTTCCTGAAGATCAAGCTAAAGCAATACGCCAGCAAGCGCCGCGCCATGAGCCTGAATAATCTCCAGCGATGCGTGAATCGCCGTTCCGTCGGTGATCACATCATCTACCAGCATTACGCTGCCTTGTACCGCGCTTCCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCTCCACGGCGGtagaggtcgcac                                                                                                                                                                                                                                        <  2:37501/247‑12 (MQ=255)          ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAAt                                                                                                                                                                                                                                      <  1:219368/248‑1 (MQ=255)             cgTTCCTGACGACCAATCGAAATCAATACGCCAGCACGCGTCGCGCCATTAGGCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGTTCACATCATCTACCAGCATTACGCTTCCTTGGAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGGCGGT‑AGTTGTCGCAATCgg                                                                                                                                                                                                                                   <  2:212786/251‑1 (MQ=255)              gTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTTAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAACGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGGCGCAATCggg                                                                                                                                                                                                                                  <  2:398648/251‑1 (MQ=255)                      cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGAAGCTCCGCCAGCGCCACGGCGGtagggggcgcaaaccgggtcccctt                                                                                                                                                                                                                           >  1:296454/1‑227 (MQ=255)                           aaGCGAAATCAATGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTTGTCGCAATCGGGATCCCTTTGTAA‑g                                                                                                                                                                                                                    <  1:398117/250‑1 (MQ=255)                                   acaatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCa                                                                                                                                                                                                            <  2:337078/246‑1 (MQ=255)                                           ccAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAAcagcag                                                                                                                                                                                                    <  1:456770/251‑1 (MQ=255)                                            cAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGGGGGGCCCATCGGGATTCCTTTGTaaaaaggggccaacaaccg                                                                                                                                                                                                     >  1:13309/1‑233 (MQ=255)                                              gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCTTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATc                                                                                                                                                                                                 <  1:493590/251‑1 (MQ=255)                                                  gCGTCGCGCCATTAGCCTGAATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAAc                                                                                                                                                                                             <  1:310817/251‑1 (MQ=255)                                                   cGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACt                                                                                                                                                                                            <  1:308877/251‑1 (MQ=255)                                                    gTCGCGCCATTAGCCTGATTAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGAGTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTc                                                                                                                                                                                           <  2:156605/251‑1 (MQ=255)                                                                gCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATc                                                                                                                                                                              <  1:231518/251‑1 (MQ=255)                                                                            cccATCGATTCACGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGGTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAACCCACCAATGCTTc                                                                                                                                                                  <  2:61416/250‑1 (MQ=255)                                                                             ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTc                                                                                                                                                                  <  1:104180/250‑1 (MQ=255)                                                                               aTCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTcggc                                                                                                                                                               <  1:154842/251‑4 (MQ=255)                                                                                      cgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑Taaaaa                                                                                                                                                       <  1:149115/251‑1 (MQ=255)                                                                                               ccGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAAc                                                                                                                                              <  1:434120/251‑1 (MQ=255)                                                                                                                         aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                                      <  1:168885/249‑1 (MQ=255)                                                                                                                               aTTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCGTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTGCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGccc                                                                                                              <  2:286113/251‑1 (MQ=255)                                                                                                                                 tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATACCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATTCCCGAATCCCCCCATGCTTCCACG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCCGGATTAAACAGCCCgg                                                                                                            >  1:440958/1‑251 (MQ=255)                                                                                                                                      gTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                                                       <  1:347966/251‑1 (MQ=255)                                                                                                                                           tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaaga                                                                                                   <  1:365302/250‑1 (MQ=255)                                                                                                                                                   gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGAGCCACGGCGGG‑AGGGGGCGGAATCCGGGTCCCTTTGTAACGC‑GGGGCAAAAAGCAGATCGGACTCA‑ATGCCCGAATCCAACAATGCTTCAGCG‑GAAAAACGGCCCAAAAGGGCCAGGGGGCGCCCGGGGTTAAAAAGCCCGGCGTTGAAAAAATAggggg                                                                                          >  1:426287/1‑250 (MQ=255)                                                                                                                                                     tACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGGGGTAGCCCTCGGGATACCTTTTTAA‑GCCGGGCCCAACACCAGATCGAA‑ACACATGCCAGAATCCACCCACGCTTCAACG‑TAAAAAACGCCTAACAGTGACACGGCGCGCCCGGTATTAAACAACCCCGCGTTTAAGAAAAAgggggct                                                                                        >  2:260458/1‑248 (MQ=255)                                                                                                                                                              gATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                                               <  1:232273/251‑1 (MQ=255)                                                                                                                                                               aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGGCGCCATCGGGATCCCCTTGTAA‑GCAGGGCCAAACAGCAGATCGAACCCC‑ATGCCCAAATCCACCAATGCTTCAGCG‑TAAAAAAGGGCTAACAGAGCCCGGGCGCGCCCGGGATTAAAAAACCCGGCGTTGAAAAAAAAGGGGGTTTTGGGCCCgg                                                                              >  2:331783/1‑251 (MQ=255)                                                                                                                                                                     gccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGT‑AGTGGTGGCACACCGGATCCCTTTTTAA‑CCCGGGCCCAACAGCCGATCCAACCCC‑ATGCCCGAATCCACCCATGCTTCACCGGAAAAAACGCCCTA‑CAGTGCCCGGGCACCCCCGGGATTACACAACCCGGCGTTGGAGAAAAAAGGGCCTTTGCGCCCGGGttttt                                                                        >  2:392618/1‑249 (MQ=255)                                                                                                                                                                         tCACCGTGGTCTTTTGACTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGACACGGCGGT‑AGGGGTCGCAAAAGGGGTCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATACACCAATGCTTCAGCG‑TAAAAACGGCCTAAAAGTGCCAGGGCGCCCCCGGCATTAAACAGCCCCGCGTTGAAGAAATAAGGGCTTTTGCGCCCGGATTTCAGCgg                                                                    >  1:89320/1‑250 (MQ=255)                                                                                                                                                                                     tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                          <  1:314314/249‑1 (MQ=255)                                                                                                                                                                                                gCGGTTCAAGCAGTCCGGCAGGTCAAGGTAATGATGCGCCGCCAGCGCCGCGGCGGT‑AGTGGTCGCAAGCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCCCCAATGCTTCAGCG‑TAAAAACGGCCTAACATTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGTCAAACTTTAACCCCt                                             <  2:266723/251‑1 (MQ=255)                                                                                                                                                                                                gCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGTCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAcc                                              <  1:438345/250‑1 (MQ=255)                                                                                                                                                                                                        cccAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCtgcttgct                                      <  2:415283/248‑1 (MQ=255)                                                                                                                                                                                                        aGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa                                     <  1:331783/251‑1 (MQ=255)                                                                                                                                                                                                                ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                              >  1:486948/1‑250 (MQ=255)                                                                                                                                                                                                                  caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCCGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                           >  2:413284/1‑251 (MQ=255)                                                                                                                                                                                                                        aaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAAAGGCCTAACAGTGCCAGGTCACGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCGTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTCAACACCTGCTTGCTAAGCGCAAATTCAATa                      >  2:406507/2‑250 (MQ=255)                                                                                                                                                                                                                                      ccGCCAGCGCCACGGCGGTATG‑GGTCGCAATCGGGATCCCGTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATGTCAGCGTAAACTCGCGAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGat         <  2:435929/249‑1 (MQ=255)                                                                                                                                                                                                                                      ccGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATCCCTATGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ACCCCAGAAGCCCCCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCTGATTTCAGCGTAAACTCGCCAAACTTGAACACCTGCTTGCTGAGCGCAAATTCAATAAACTTGCGCTGat         <  2:21061/249‑1 (MQ=255)                                                                                                                                                                                                                                           agcgCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGttt  >  1:495371/4‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       TCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTT  >  NC_000913/3815281‑3815765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑