Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,517	T→C	A84A (GCA→GCG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,517	0	T	C	97.7%	137.7 / NA	43	A84A (GCA→GCG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (12/30);  minor base A (1/0);  total (13/30)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGAT  >  NC_000913/3815272‑3815758                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gCCGAAATCTCGCCGCGCCCCCGTTCCTGCCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCACGGTCTTGATGCCCCCCCAGCGcccc                                                                                                                                                                                                                                                  >  1:156605/1‑249 (MQ=255)     aaaTCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGATCCGCCAGCGccccgctg                                                                                                                                                                                                                                              >  1:438828/1‑245 (MQ=255)          ccgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtagt                                                                                                                                                                                                                                         <  1:454557/250‑4 (MQ=255)                  cccccgTTCCTGAAGATCAAGCTAAAGCAATACGCCAGCAAGCGCCGCGCCATGAGCCTGAATAATCTCCAGCGATGCGTGAATCGCCGTTCCGTCGGTGATCACATCATCTACCAGCATTACGCTGCCTTGTACCGCGCTTCCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCTCCACGGCGGtagaggtcgcac                                                                                                                                                                                                                                 <  2:37501/247‑12 (MQ=255)                   ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAAt                                                                                                                                                                                                                               <  1:219368/248‑1 (MQ=255)                      cgTTCCTGACGACCAATCGAAATCAATACGCCAGCACGCGTCGCGCCATTAGGCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGTTCACATCATCTACCAGCATTACGCTTCCTTGGAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGGCGGT‑AGTTGTCGCAATCgg                                                                                                                                                                                                                            <  2:212786/251‑1 (MQ=255)                       gTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTTAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAACGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGGCGCAATCggg                                                                                                                                                                                                                           <  2:398648/251‑1 (MQ=255)                               cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGAAGCTCCGCCAGCGCCACGGCGGtagggggcgcaaaccgggtcccctt                                                                                                                                                                                                                    >  1:296454/1‑227 (MQ=255)                                    aaGCGAAATCAATGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTTGTCGCAATCGGGATCCCTTTGTAA‑g                                                                                                                                                                                                             <  1:398117/250‑1 (MQ=255)                                            acaatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCa                                                                                                                                                                                                     <  2:337078/246‑1 (MQ=255)                                                    ccAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAAcagcag                                                                                                                                                                                             <  1:456770/251‑1 (MQ=255)                                                     cAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGGGGGGCCCATCGGGATTCCTTTGTaaaaaggggccaacaaccg                                                                                                                                                                                              >  1:13309/1‑233 (MQ=255)                                                       gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCTTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATc                                                                                                                                                                                          <  1:493590/251‑1 (MQ=255)                                                           gCGTCGCGCCATTAGCCTGAATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAAc                                                                                                                                                                                      <  1:310817/251‑1 (MQ=255)                                                            cGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACt                                                                                                                                                                                     <  1:308877/251‑1 (MQ=255)                                                             gTCGCGCCATTAGCCTGATTAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGAGTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTc                                                                                                                                                                                    <  2:156605/251‑1 (MQ=255)                                                                         gCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATc                                                                                                                                                                       <  1:231518/251‑1 (MQ=255)                                                                                     cccATCGATTCACGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGGTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAACCCACCAATGCTTc                                                                                                                                                           <  2:61416/250‑1 (MQ=255)                                                                                      ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTc                                                                                                                                                           <  1:104180/250‑1 (MQ=255)                                                                                        aTCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTcggc                                                                                                                                                        <  1:154842/251‑4 (MQ=255)                                                                                               cgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑Taaaaa                                                                                                                                                <  1:149115/251‑1 (MQ=255)                                                                                                        ccGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAAc                                                                                                                                       <  1:434120/251‑1 (MQ=255)                                                                                                                                  aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                               <  1:168885/249‑1 (MQ=255)                                                                                                                                        aTTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCGTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTGCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGccc                                                                                                       <  2:286113/251‑1 (MQ=255)                                                                                                                                          tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATACCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATTCCCGAATCCCCCCATGCTTCCACG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCCGGATTAAACAGCCCgg                                                                                                     >  1:440958/1‑251 (MQ=255)                                                                                                                                               gTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                                                <  1:347966/251‑1 (MQ=255)                                                                                                                                                    tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaaga                                                                                            <  1:365302/250‑1 (MQ=255)                                                                                                                                                            gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGAGCCACGGCGGG‑AGGGGGCGGAATCCGGGTCCCTTTGTAACGC‑GGGGCAAAAAGCAGATCGGACTCA‑ATGCCCGAATCCAACAATGCTTCAGCG‑GAAAAACGGCCCAAAAGGGCCAGGGGGCGCCCGGGGTTAAAAAGCCCGGCGTTGAAAAAATAggggg                                                                                   >  1:426287/1‑250 (MQ=255)                                                                                                                                                              tACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGGGGTAGCCCTCGGGATACCTTTTTAA‑GCCGGGCCCAACACCAGATCGAA‑ACACATGCCAGAATCCACCCACGCTTCAACG‑TAAAAAACGCCTAACAGTGACACGGCGCGCCCGGTATTAAACAACCCCGCGTTTAAGAAAAAgggggct                                                                                 >  2:260458/1‑248 (MQ=255)                                                                                                                                                                       gATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                                        <  1:232273/251‑1 (MQ=255)                                                                                                                                                                        aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGG‑AGTGGGCGCCATCGGGATCCCCTTGTAA‑GCAGGGCCAAACAGCAGATCGAACCCC‑ATGCCCAAATCCACCAATGCTTCAGCG‑TAAAAAAGGGCTAACAGAGCCCGGGCGCGCCCGGGATTAAAAAACCCGGCGTTGAAAAAAAAGGGGGTTTTGGGCCCgg                                                                       >  2:331783/1‑251 (MQ=255)                                                                                                                                                                              gccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGT‑AGTGGTGGCACACCGGATCCCTTTTTAA‑CCCGGGCCCAACAGCCGATCCAACCCC‑ATGCCCGAATCCACCCATGCTTCACCGGAAAAAACGCCCTA‑CAGTGCCCGGGCACCCCCGGGATTACACAACCCGGCGTTGGAGAAAAAAGGGCCTTTGCGCCCGGGttttt                                                                 >  2:392618/1‑249 (MQ=255)                                                                                                                                                                                  tCACCGTGGTCTTTTGACTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGACACGGCGGT‑AGGGGTCGCAAAAGGGGTCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATACACCAATGCTTCAGCG‑TAAAAACGGCCTAAAAGTGCCAGGGCGCCCCCGGCATTAAACAGCCCCGCGTTGAAGAAATAAGGGCTTTTGCGCCCGGATTTCAGCgg                                                             >  1:89320/1‑250 (MQ=255)                                                                                                                                                                                              tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                                   <  1:314314/249‑1 (MQ=255)                                                                                                                                                                                                         gCGGTTCAAGCAGTCCGGCAGGTCAAGGTAATGATGCGCCGCCAGCGCCGCGGCGGT‑AGTGGTCGCAAGCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCCCCAATGCTTCAGCG‑TAAAAACGGCCTAACATTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGTCAAACTTTAACCCCt                                      <  2:266723/251‑1 (MQ=255)                                                                                                                                                                                                         gCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGTCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAcc                                       <  1:438345/250‑1 (MQ=255)                                                                                                                                                                                                                 cccAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCtgcttgct                               <  2:415283/248‑1 (MQ=255)                                                                                                                                                                                                                 aGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa                              <  1:331783/251‑1 (MQ=255)                                                                                                                                                                                                                         ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa                       >  1:486948/1‑250 (MQ=255)                                                                                                                                                                                                                           caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCCGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt                    >  2:413284/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaggtcATGATGCTCCGCCAGCGCCACGGCGGT‑AGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAAAGGCCTAACAGTGCCAGGTCACGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCGTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTCAACACCTGCTTGCTAAGCGCAAATTCAATa               >  2:406507/2‑250 (MQ=255)                                                                                                                                                                                                                                               ccGCCAGCGCCACGGCGGTATG‑GGTCGCAATCGGGATCCCGTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATGTCAGCGTAAACTCGCGAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGat  <  2:435929/249‑1 (MQ=255)                                                                                                                                                                                                                                               ccGCCAGCGCCACGGCGGG‑AGTGGTCGCAATCGGGATCCCTATGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ACCCCAGAAGCCCCCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCTGATTTCAGCGTAAACTCGCCAAACTTGAACACCTGCTTGCTGAGCGCAAATTCAATAAACTTGCGCTGat  <  2:21061/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGT‑TGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGAT  >  NC_000913/3815272‑3815758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑