Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,502 G→A H89H (CAC→CAT)  pyrE ← orotate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,5020GA95.7% 148.1 / ‑5.8 48H89H (CAC→CAT) pyrEorotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/33);  minor base T (1/1);  total (14/34)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATA  >  NC_000913/3815256‑3815745
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
cccAACGTCCTGAATCGCCGAAACCTCGCCGCGCCCCCGTTCCTGACAGGCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCAGTAGCCTGAATAATCTCCAGCTATGCGCGAATCGCCGTTCCGGCGGTGATCACAGCATCTGCCAGCATTACGCGTCCGTGTAACGCGCTACCATCCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAATCAGTACGGCTGGTCAAGGTCATGAt                                                                                                                                                                                                                                                    <  2:455165/249‑1 (MQ=255)
       accTGAATCGCCTAAATCTCGCCGCGCCCCCTTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTTGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGc                                                                                                                                                                                                                                             <  1:320372/250‑1 (MQ=255)
        ccTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGcc                                                                                                                                                                                                                                            <  1:300534/251‑1 (MQ=255)
        ccTGAATCGCCGAAATCTCGCCGCGCACCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTACTGATGCTCCGcc                                                                                                                                                                                                                                            >  1:21061/1‑251 (MQ=255)
          ggAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAg                                                                                                                                                                                                                                          <  2:204904/250‑1 (MQ=255)
           gAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgc                                                                                                                                                                                                                                         <  1:110792/251‑2 (MQ=255)
           aaaTCGCCGAAATCTCGCCGCGCCCCCGTCCCTTACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgc                                                                                                                                                                                                                                         <  2:160135/250‑2 (MQ=255)
                gCCGAAATCTCGCCGCGCCCCCGTTCCTGCCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCACGGTCTTGATGCCCCCCCAGCGcccc                                                                                                                                                                                                                                    >  1:156605/1‑249 (MQ=255)
                    aaaTCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGATCCGCCAGCGccccgctg                                                                                                                                                                                                                                >  1:438828/1‑245 (MQ=255)
                         ccgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtagt                                                                                                                                                                                                                           <  1:454557/250‑4 (MQ=255)
                                 cccccgTTCCTGAAGATCAAGCTAAAGCAATACGCCAGCAAGCGCCGCGCCATGAGCCTGAATAATCTCCAGCGATGCGTGAATCGCCGTTCCGTCGGTGATCACATCATCTACCAGCATTACGCTGCCTTGTACCGCGCTTCCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCTCCACGGCGGtagaggtcgcac                                                                                                                                                                                                                   <  2:37501/247‑12 (MQ=255)
                                  ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAAt                                                                                                                                                                                                                  <  1:219368/248‑1 (MQ=255)
                                     cgTTCCTGACGACCAATCGAAATCAATACGCCAGCACGCGTCGCGCCATTAGGCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGTTCACATCATCTACCAGCATTACGCTTCCTTGGAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGGCGGTAGTTGTCGCAATCgg                                                                                                                                                                                                               <  2:212786/251‑1 (MQ=255)
                                      gTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTTAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAACGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGGCGCAATCggg                                                                                                                                                                                                              <  2:398648/251‑1 (MQ=255)
                                              cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGAAGCTCCGCCAGCGCCACGGCGGtagggggcgcaaaccgggtcccctt                                                                                                                                                                                                      >  1:296454/1‑227 (MQ=255)
                                                   aaGCGAAATCAATGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTTGTCGCAATCGGGATCCCTTTGTAA‑g                                                                                                                                                                                                <  1:398117/250‑1 (MQ=255)
                                                           acaatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCa                                                                                                                                                                                        <  2:337078/246‑1 (MQ=255)
                                                                   ccAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAAcagcag                                                                                                                                                                                <  1:456770/251‑1 (MQ=255)
                                                                    cAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGGGCCCATCGGGATTCCTTTGTaaaaaggggccaacaaccg                                                                                                                                                                                 >  1:13309/1‑233 (MQ=255)
                                                                      gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCTTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATc                                                                                                                                                                             <  1:493590/251‑1 (MQ=255)
                                                                          gCGTCGCGCCATTAGCCTGAATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAAc                                                                                                                                                                         <  1:310817/251‑1 (MQ=255)
                                                                           cGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACt                                                                                                                                                                        <  1:308877/251‑1 (MQ=255)
                                                                            gTCGCGCCATTAGCCTGATTAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGAGTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTc                                                                                                                                                                       <  2:156605/251‑1 (MQ=255)
                                                                                        gCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATc                                                                                                                                                          <  1:231518/251‑1 (MQ=255)
                                                                                                    cccATCGATTCACGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGGTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAACCCACCAATGCTTc                                                                                                                                              <  2:61416/250‑1 (MQ=255)
                                                                                                     ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTc                                                                                                                                              <  1:104180/250‑1 (MQ=255)
                                                                                                       aTCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTcggc                                                                                                                                           <  1:154842/251‑4 (MQ=255)
                                                                                                              cgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑Taaaaa                                                                                                                                   <  1:149115/251‑1 (MQ=255)
                                                                                                                       ccGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAAc                                                                                                                          <  1:434120/251‑1 (MQ=255)
                                                                                                                                                 aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                                  <  1:168885/249‑1 (MQ=255)
                                                                                                                                                       aTTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCGTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTGCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGccc                                                                                          <  2:286113/251‑1 (MQ=255)
                                                                                                                                                         tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGTCGCAATCGGGATACCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATTCCCGAATCCCCCCATGCTTCCACG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCCGGATTAAACAGCCCgg                                                                                        >  1:440958/1‑251 (MQ=255)
                                                                                                                                                              gTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                                   <  1:347966/251‑1 (MQ=255)
                                                                                                                                                                   tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaaga                                                                               <  1:365302/250‑1 (MQ=255)
                                                                                                                                                                           gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGAGCCACGGCGGGAGGGGGCGGAATCCGGGTCCCTTTGTAACGC‑GGGGCAAAAAGCAGATCGGACTCA‑ATGCCCGAATCCAACAATGCTTCAGCG‑GAAAAACGGCCCAAAAGGGCCAGGGGGCGCCCGGGGTTAAAAAGCCCGGCGTTGAAAAAATAggggg                                                                      >  1:426287/1‑250 (MQ=255)
                                                                                                                                                                             tACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTAGCCCTCGGGATACCTTTTTAA‑GCCGGGCCCAACACCAGATCGAA‑ACACATGCCAGAATCCACCCACGCTTCAACG‑TAAAAAACGCCTAACAGTGACACGGCGCGCCCGGTATTAAACAACCCCGCGTTTAAGAAAAAgggggct                                                                    >  2:260458/1‑248 (MQ=255)
                                                                                                                                                                                      gATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                           <  1:232273/251‑1 (MQ=255)
                                                                                                                                                                                       aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCCATCGGGATCCCCTTGTAA‑GCAGGGCCAAACAGCAGATCGAACCCC‑ATGCCCAAATCCACCAATGCTTCAGCG‑TAAAAAAGGGCTAACAGAGCCCGGGCGCGCCCGGGATTAAAAAACCCGGCGTTGAAAAAAAAGGGGGTTTTGGGCCCgg                                                          >  2:331783/1‑251 (MQ=255)
                                                                                                                                                                                             gccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGTAGTGGTGGCACACCGGATCCCTTTTTAA‑CCCGGGCCCAACAGCCGATCCAACCCC‑ATGCCCGAATCCACCCATGCTTCACCGGAAAAAACGCCCTA‑CAGTGCCCGGGCACCCCCGGGATTACACAACCCGGCGTTGGAGAAAAAAGGGCCTTTGCGCCCGGGttttt                                                    >  2:392618/1‑249 (MQ=255)
                                                                                                                                                                                                 tCACCGTGGTCTTTTGACTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGACACGGCGGTAGGGGTCGCAAAAGGGGTCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATACACCAATGCTTCAGCG‑TAAAAACGGCCTAAAAGTGCCAGGGCGCCCCCGGCATTAAACAGCCCCGCGTTGAAGAAATAAGGGCTTTTGCGCCCGGATTTCAGCgg                                                >  1:89320/1‑250 (MQ=255)
                                                                                                                                                                                                             tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                      <  1:314314/249‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGTTCAAGCAGTCCGGCAGGTCAAGGTAATGATGCGCCGCCAGCGCCGCGGCGGTAGTGGTCGCAAGCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCCCCAATGCTTCAGCG‑TAAAAACGGCCTAACATTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGTCAAACTTTAACCCCt                         <  2:266723/251‑1 (MQ=255)
                                                                                                                                                                                                                        gCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGTCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAcc                          <  1:438345/250‑1 (MQ=255)
                                                                                                                                                                                                                                cccAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCtgcttgct                  <  2:415283/248‑1 (MQ=255)
                                                                                                                                                                                                                                aGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa                 <  1:331783/251‑1 (MQ=255)
                                                                                                                                                                                                                                        ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa          >  1:486948/1‑250 (MQ=255)
                                                                                                                                                                                                                                          caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCCGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt       >  2:413284/1‑251 (MQ=255)
                                                                                                                                                                                                                                                aaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAAAGGCCTAACAGTGCCAGGTCACGCCCGGAATTAAACAGCCCGGCGTTGAAGAAATAGGGGCGTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTCAACACCTGCTTGCTAAGCGCAAATTCAATa  >  2:406507/2‑250 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
CTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATA  >  NC_000913/3815256‑3815745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑