Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,496	C→A	L91L (CTG→CTT)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,496	0	C	A	97.8%	148.3 / NA	46	L91L (CTG→CTT)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (11/34);  total (12/34)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATT  >  NC_000913/3815256‑3815740                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        cccAACGTCCTGAATCGCCGAAACCTCGCCGCGCCCCCGTTCCTGACAGGCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCAGTAGCCTGAATAATCTCCAGCTATGCGCGAATCGCCGTTCCGGCGGTGATCACAGCATCTGCCAGCATTACGCGTCCGTGTAACGCGCTACCATCCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAATCAGTACGGCTGGTCAAGGTCATGAt                                                                                                                                                                                                                                               <  2:455165/249‑1 (MQ=255)  tCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCCGCATTACGCGGCCTTGTAACGCGCCACCAACCAGATTGCCGCATTCCCCGTGGCCTTTTGCCTCTTTGCGGNTAAAGCAGTACGGcagggccaagtaactatt                                                                                                                                                                                                                                              >  2:219928/1‑241 (MQ=255)        accTGAATCGCCTAAATCTCGCCGCGCCCCCTTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTTGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGc                                                                                                                                                                                                                                        <  1:320372/250‑1 (MQ=255)         ccTGAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGcc                                                                                                                                                                                                                                       <  1:300534/251‑1 (MQ=255)         ccTGAATCGCCGAAATCTCGCCGCGCACCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGGCAAGGTACTGATGCTCCGcc                                                                                                                                                                                                                                       >  1:21061/1‑251 (MQ=255)           ggAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAg                                                                                                                                                                                                                                     <  2:204904/250‑1 (MQ=255)            gAATCGCCGAAATCTCGCCGCGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgc                                                                                                                                                                                                                                    <  1:110792/251‑2 (MQ=255)            aaaTCGCCGAAATCTCGCCGCGCCCCCGTCCCTTACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAgc                                                                                                                                                                                                                                    <  2:160135/250‑2 (MQ=255)                 gCCGAAATCTCGCCGCGCCCCCGTTCCTGCCGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGGGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCACGGTCTTGATGCCCCCCCAGCGcccc                                                                                                                                                                                                                               >  1:156605/1‑249 (MQ=255)                     aaaTCTCGCCGCGCCCCCGTTCCTGACGCTCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGAGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGATCCGCCAGCGccccgctg                                                                                                                                                                                                                           >  1:438828/1‑245 (MQ=255)                          ccgccgcGCCCCCGTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGtagt                                                                                                                                                                                                                      <  1:454557/250‑4 (MQ=255)                                  cccccgTTCCTGAAGATCAAGCTAAAGCAATACGCCAGCAAGCGCCGCGCCATGAGCCTGAATAATCTCCAGCGATGCGTGAATCGCCGTTCCGTCGGTGATCACATCATCTACCAGCATTACGCTGCCTTGTACCGCGCTTCCAACCAGATTGCCGCCTTCTCCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCTCCACGGCGGtagaggtcgcac                                                                                                                                                                                                              <  2:37501/247‑12 (MQ=255)                                   ccccgTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAAt                                                                                                                                                                                                             <  1:219368/248‑1 (MQ=255)                                      cgTTCCTGACGACCAATCGAAATCAATACGCCAGCACGCGTCGCGCCATTAGGCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGTTCACATCATCTACCAGCATTACGCTTCCTTGGAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGTCAGGTCAAGGTCTTGATGCTCCGCCAGCGCCACGGCGGTAGTTGTCGCAATCgg                                                                                                                                                                                                          <  2:212786/251‑1 (MQ=255)                                       gTTCCTGACGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTTAATAATCGCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAACGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGGCGCAATCggg                                                                                                                                                                                                         <  2:398648/251‑1 (MQ=255)                                               cGATCAAGCGAAATCAATACGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGGCATGAAGCTCCGCCAGCGCCACGGCGGtagggggcgcaaaccgggtcccctt                                                                                                                                                                                                 >  1:296454/1‑227 (MQ=255)                                                    aaGCGAAATCAATGCGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACTCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTTGTCGCAATCGGGATCCCTTTGTAA‑g                                                                                                                                                                                           <  1:398117/250‑1 (MQ=255)                                                            acaatacGCCAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCa                                                                                                                                                                                   <  2:337078/246‑1 (MQ=255)                                                                    ccAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAAcagcag                                                                                                                                                                           <  1:456770/251‑1 (MQ=255)                                                                     cAGCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGGGGGGCCCATCGGGATTCCTTTGTaaaaaggggccaacaaccg                                                                                                                                                                            >  1:13309/1‑233 (MQ=255)                                                                       gCAAGCGTCGCGCCATTAGCCTGAATAATCTCCATCGATTCGCGAATCGCCTTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATc                                                                                                                                                                        <  1:493590/251‑1 (MQ=255)                                                                           gCGTCGCGCCATTAGCCTGAATAATCACCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAAc                                                                                                                                                                    <  1:310817/251‑1 (MQ=255)                                                                            cGTCGCGCCATTAGCCTTAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACt                                                                                                                                                                   <  1:308877/251‑1 (MQ=255)                                                                             gTCGCGCCATTAGCCTGATTAACCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGAGTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTc                                                                                                                                                                  <  2:156605/251‑1 (MQ=255)                                                                                         gCCTGAATAATCTCCATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATc                                                                                                                                                     <  1:231518/251‑1 (MQ=255)                                                                                                     cccATCGATTCACGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCCCCGTGGTCTTTTGCCGCTTTGCGGGTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAACCCACCAATGCTTc                                                                                                                                         <  2:61416/250‑1 (MQ=255)                                                                                                      ccATCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTc                                                                                                                                         <  1:104180/250‑1 (MQ=255)                                                                                                        aTCGATTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTcggc                                                                                                                                      <  1:154842/251‑4 (MQ=255)                                                                                                               cgcgAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑Taaaaa                                                                                                                              <  1:149115/251‑1 (MQ=255)                                                                                                                        ccGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAAc                                                                                                                     <  1:434120/251‑1 (MQ=255)                                                                                                                                                  aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTa                                                                                             <  1:168885/249‑1 (MQ=255)                                                                                                                                                        aTTACGCGTCCTTGTAACGCGCTACCAACCAGATGGCCGCCGTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTGCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGccc                                                                                     <  2:286113/251‑1 (MQ=255)                                                                                                                                                          tACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGTCGCAATCGGGATACCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATTCCCGAATCCCCCCATGCTTCCACG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCCGGATTAAACAGCCCgg                                                                                   >  1:440958/1‑251 (MQ=255)                                                                                                                                                               gTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTg                                                                              <  1:347966/251‑1 (MQ=255)                                                                                                                                                                    tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTgaaga                                                                          <  1:365302/250‑1 (MQ=255)                                                                                                                                                                            gcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGAGCCACGGCGGGAGGGGGCGGAATCCGGGTCCCTTTGTAACGC‑GGGGCAAAAAGCAGATCGGACTCA‑ATGCCCGAATCCAACAATGCTTCAGCG‑GAAAAACGGCCCAAAAGGGCCAGGGGGCGCCCGGGGTTAAAAAGCCCGGCGTTGAAAAAATAggggg                                                                 >  1:426287/1‑250 (MQ=255)                                                                                                                                                                              tACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGGGGTAGCCCTCGGGATACCTTTTTAA‑GCCGGGCCCAACACCAGATCGAA‑ACACATGCCAGAATCCACCCACGCTTCAACG‑TAAAAAACGCCTAACAGTGACACGGCGCGCCCGGTATTAAACAACCCCGCGTTTAAGAAAAAgggggct                                                               >  2:260458/1‑248 (MQ=255)                                                                                                                                                                                       gATTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCg                                                      <  1:232273/251‑1 (MQ=255)                                                                                                                                                                                        aTTGCCGCCTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGGAGTGGGCGCCATCGGGATCCCCTTGTAA‑GCAGGGCCAAACAGCAGATCGAACCCC‑ATGCCCAAATCCACCAATGCTTCAGCG‑TAAAAAAGGGCTAACAGAGCCCGGGCGCGCCCGGGATTAAAAAACCCGGCGTTGAAAAAAAAGGGGGTTTTGGGCCCgg                                                     >  2:331783/1‑251 (MQ=255)                                                                                                                                                                                              gccTTCACCGTGGTCTTTTGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCATGATGCTCCGCCCGCGCCACGGCGGTAGTGGTGGCACACCGGATCCCTTTTTAA‑CCCGGGCCCAACAGCCGATCCAACCCC‑ATGCCCGAATCCACCCATGCTTCACCGGAAAAAACGCCCTA‑CAGTGCCCGGGCACCCCCGGGATTACACAACCCGGCGTTGGAGAAAAAAGGGCCTTTGCGCCCGGGttttt                                               >  2:392618/1‑249 (MQ=255)                                                                                                                                                                                                  tCACCGTGGTCTTTTGACTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGACACGGCGGTAGGGGTCGCAAAAGGGGTCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATACACCAATGCTTCAGCG‑TAAAAACGGCCTAAAAGTGCCAGGGCGCCCCCGGCATTAAACAGCCCCGCGTTGAAGAAATAAGGGCTTTTGCGCCCGGATTTCAGCgg                                           >  1:89320/1‑250 (MQ=255)                                                                                                                                                                                                              tttGCCTCTTTGCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCa                                 <  1:314314/249‑1 (MQ=255)                                                                                                                                                                                                                         gCGGTTCAAGCAGTCCGGCAGGTCAAGGTAATGATGCGCCGCCAGCGCCGCGGCGGTAGTGGTCGCAAGCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCCCCAATGCTTCAGCG‑TAAAAACGGCCTAACATTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGTCAAACTTTAACCCCt                    <  2:266723/251‑1 (MQ=255)                                                                                                                                                                                                                         gCGGTTAAAGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGTCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGGTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAcc                     <  1:438345/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCtgcttgct             <  2:415283/248‑1 (MQ=255)                                                                                                                                                                                                                                 aGCAGTACGGCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTa            <  1:331783/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggCAGGTCAAGGTCATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCAGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCaa     >  1:486948/1‑250 (MQ=255)                                                                                                                                                                                                                                           caggtcaaggtcATGATGCTCCGCCAGCGCCACGGCGGTAGTGGTCGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCCGAATCCACCAATGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAAtt  >  2:413284/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        CTCAACTTCCTGAATCGCCGAAATCTCGCCGCGCCCGCGTTCCTGACGATCGAGCGAAATCAACACGCCAGCAAGCGTCGCGCCATTGGCCTGAATAATCTCCATCGACTCGCGAATCGCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAA‑GCAGGGCCAAACAGCAGATCGAACTCA‑ATGCCGGAATCCACCAACGCTTCAGCG‑TAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATT  >  NC_000913/3815256‑3815740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑