Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,768,988	C→T	intergenic (+350/‑357)	yibG → / → yibV	tetratricopeptide‑like domain‑containing protein YibG/PF15596 family protein YibV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,768,988	0	C	T	100.0%	117.3 / NA	32	intergenic (+350/‑357)	yibG/yibV	tetratricopeptide‑like domain‑containing protein YibG/PF15596 family protein YibV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/15);  total (17/15)

CGATTGGGCTGGAAGGTAGGTGGAATTTTTATCAGTATCCGTTGAACCCGATTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCCAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCAAAAATTAAATGAGTTAATAT  >  NC_000913/3768751‑3769218                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       cGATTGGGCTGGAAGGTAGGTGGAATTTTTATCAGTATCCGTTGAACCCGATTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGc                                                                                                                                                                                                                           <  1:138098/251‑1 (MQ=255)           ggAAGGTAGGTGGAATTTTTATCAGTATCCGTTGAACCCGATTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAg                                                                                                                                                                                                                   <  1:420931/249‑1 (MQ=255)                            tttATCAGTATCCGTTGAACACGATTTCTGGAATCGATCCCCTTGTGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTGCACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGAccc                                                                                                                                                                                                 <  2:263325/250‑1 (MQ=255)                                  aGTATCCGTTGAACCCGATTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGagc                                                                                                                                                                                           >  1:113035/1‑250 (MQ=255)                                                   aTTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTg                                                                                                                                                                          >  1:112748/1‑250 (MQ=255)                                                     ttCTGGAAGCGATCCCCTTGGGATTAGCCACCTGTTTATCCAGCATAACTTTAGGTATTCTTGCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCTCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGTCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAGCCTGAc                                                                                                                                                                        <  2:498795/250‑1 (MQ=255)                                                         ggAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCAt                                                                                                                                                                   >  1:361171/1‑251 (MQ=255)                                                                 tCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCCTATGAtt                                                                                                                                                             >  2:268921/1‑249 (MQ=255)                                                                  ccccTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAg                                                                                                                                                           <  1:8012/250‑1 (MQ=255)                                                                                                   aaCTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCa                                                                                                                          <  1:194513/250‑1 (MQ=255)                                                                                                                  ttCGAGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATAc                                                                                                           >  1:406267/1‑250 (MQ=255)                                                                                                                    cGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATa                                                                                                        <  2:121347/251‑1 (MQ=255)                                                                                                                    cGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAAGACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACGTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATa                                                                                                        <  1:229304/251‑1 (MQ=255)                                                                                                                                    aCCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCAt                                                                                                                                                                   <  2:221775/176‑1 (MQ=255)                                                                                                                                    aCCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCAt                                                                                                                                                                   >  1:221775/1‑176 (MQ=255)                                                                                                                                          tgatgaTAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTcc                                                                                  >  1:115640/1‑251 (MQ=255)                                                                                                                                            atgatAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATA‑TCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTc                                                                               >  1:346056/1‑251 (MQ=255)                                                                                                                                                    ttttATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACATGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATACTCGCAATGGATTTTCAAAAAATAGTTTTTTAAACGCTTTTGGACCGTCTTTAgg                                                                         >  2:290676/1‑250 (MQ=255)                                                                                                                                                            tgtgCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGa                                                                                                                                                                                                                    <  1:171540/103‑1 (MQ=255)                                                                                                                                                            tgtgCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGa                                                                                                                                                                                                                    >  2:171540/1‑103 (MQ=255)                                                                                                                                                                      aTTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCg                                                        >  1:195212/1‑249 (MQ=255)                                                                                                                                                                                        aataatATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGttt                                     <  1:237703/250‑1 (MQ=255)                                                                                                                                                                                                  aaTGTAAAAATAATCCCCGTTGTACACATTTATAAGTAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACa                          <  1:337208/251‑1 (MQ=255)                                                                                                                                                                                                    tGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGt                                       <  1:252304/236‑1 (MQ=255)                                                                                                                                                                                                    tGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACATCGATTCTGTTCCGNGGGTTGTATAACTGGt                                       >  2:252304/1‑236 (MQ=255)                                                                                                                                                                                                     gTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAgttgtt                                                                                                  >  1:9385/1‑176 (MQ=255)                                                                                                                                                                                                     gTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAgttgtt                                                                                                  <  2:9385/176‑1 (MQ=255)                                                                                                                                                                                                       aaaaaTAATCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCaa                     <  2:303109/251‑1 (MQ=255)                                                                                                                                                                                                          aataatCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCaaaa                   >  1:60869/1‑250 (MQ=255)                                                                                                                                                                                                             aatCCCCGTTGTACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCAAAAAtt                >  2:89515/1‑250 (MQ=255)                                                                                                                                                                                                              atCCCCGTTGCACACATTTATAAATAGATGGCTAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCAACAATTa               >  1:243481/1‑250 (MQ=255)                                                                                                                                                                                                                          cacaTTTATAAATAGATTGCTAATAACTCAAGGCCTATGGTGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGCTTCTTTTCCGAGGTTTTTATACCTGGTTTATTNATTGACATGCCAAATTTAAATGAGTTAatat  >  2:228372/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       CGATTGGGCTGGAAGGTAGGTGGAATTTTTATCAGTATCCGTTGAACCCGATTTCTGGAATCGATCCCCTTGGGATTAGCCACCTGTTTATACAGCATAACTTTAGGTATGCTTTCGTGTGTTTCAGATATACCATGTGATGATAATTTTTATGATGTGCTTAGTATTCCAGTCGCTTCTGGTAATAATATGCAATGTAAAAATAATCCCCGTTGTACACATTTATAAATAGATGGCCAATACCTCAAGGCCTATGGAGTTGGAATGTTAATGACCCGGGAGCAAGCAATAGAAAACCTGACGGCATACGATTAGTACCGTCAGTTAATACAGGAACTTATAATCGCAATGGATTTTCAATACATAGTTGTTTAAACGCTTTTGGTCCGTCTTTAGGACCTCGATTCTGTTCCGAGGGTTGTATAACTGGTTTATCTAATGACATGCAAAAATTAAATGAGTTAATAT  >  NC_000913/3768751‑3769218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑