Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,782	T→C	intergenic (‑70/‑105)	ysaB ← / → wecH	putative lipoprotein YsaB/O‑acetyltransferase WecH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,782	0	T	C	100.0%	142.4 / NA	43	intergenic (‑70/‑105)	ysaB/wecH	putative lipoprotein YsaB/O‑acetyltransferase WecH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/25);  total (18/25)

GATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGATGCAGCCCAAAATTTACTGGATTGATAACCTGCGAGGGATAGCGTGTTTAATGGTGGTGATGATTCACACCACTACCTGGTATGTGACCAATGCTCATAGTGTTAGCCCCGTCACATGGGATATCGCCAATGTTCTGA  >  NC_000913/3725536‑3726025                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gagtttttGCTCGCCGGTGTTATAACGTTTTGCCCCCTGATCCTTGCATAACGCTTCCATATTCACCGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCt                                                                                                                                                                                                                                                 <  2:325990/248‑1 (MQ=255)         gCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCctc                                                                                                                                                                                                                                         <  2:368080/251‑1 (MQ=255)               cGGTGTTATAACGTCTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAg                                                                                                                                                                                                                                   <  1:180716/251‑1 (MQ=255)                     tataACGTTTTGCCGCCTGATCCTTGCATATCGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTa                                                                                                                                                                                                                              <  1:195895/250‑1 (MQ=255)                     tataACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGt                                                                                                                                                                                                                               >  1:299033/1‑249 (MQ=255)                         aCGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt                                                                                                                                                                                                                         <  2:364598/251‑1 (MQ=255)                           gTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATa                                                                                                                                                                                                                        >  2:188739/1‑250 (MQ=255)                                 ccgccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGCTATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTc                                                                                                                                                                                                                  >  2:72324/1‑250 (MQ=255)                                           cTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAATCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTcc                                                                                                                                                                                                        >  2:259975/1‑250 (MQ=255)                                                     gCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTACAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGccaac                                                                                                                                                                                             <  2:166608/251‑1 (MQ=255)                                                       ttCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGTTATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAATGATAAAACTCAGATTATTCCCGTGCGccaacc                                                                                                                                                                                            >  2:27733/1‑250 (MQ=255)                                                       ttCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGccaacc                                                                                                                                                                                            >  1:308953/1‑250 (MQ=255)                                                         ccatagttaacgaacgCATAGGATCAACCTTTACTCGTTGTGGTTGCTGCACGGGGTACGGTATAGAACAACCAACTAGAACCATAAGCGACATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACTGTCTGGTTTGCAAGTGAGTCTTATTATGTATATCTGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCGATAAGAAGTAGTGATAACGCGCAGATTATTCCATTGCGcctaccaa                                                                                                                                                                                          <  2:418304/242‑1 (MQ=255)                                                                    gaacgCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATNATTCCAGTGCGCCAACCAAATGATTTATCgg                                                                                                                                                                              >  2:219383/1‑251 (MQ=255)                                                                        gCAGAGTATCAACCTTTTCCCGTTGTTCGTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTGATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAg                                                                                                                                                                          <  2:9986/251‑1 (MQ=255)                                                                         cAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTCTAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCatc                                                                                                                                                                         >  2:458652/1‑249 (MQ=255)                                                                                  aatcgttACCTGTTGTGCTTTATGCACGGGGTACGGTATAGAACAGCCCACTAGCATCATAAGCGCCCTTGCCGGAAAGAAAGTGTTCATCATCATATAATGACCGTCTTGTTTGCAAGTCAGTCTTATTATTTATATCGGTATAGTGGCTTATATCTTACGAATCTCCTTAAGCCTCTATAAGAGATAGTGATAAAACTCATATTATTCCAGTGCGCCAACCAAATCTTTTATCGGTCAGACCTTTTTTa                                                                                                                                                                <  2:157173/246‑1 (MQ=255)                                                                                         aCCCGTTGTGCTTTCTGCACTGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGAt                                                                                                                                                         <  2:8893/251‑1 (MQ=255)                                                                                          cccGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCATATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATa                                                                                                                                                        <  2:149276/251‑1 (MQ=255)                                                                                           ccGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATAc                                                                                                                                                       >  2:132426/1‑251 (MQ=255)                                                                                             gTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGAGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCACGTGAGTCTTATTATTTATATCGGTATAGAGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGCGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATAc                                                                                                                                                       >  1:218668/1‑249 (MQ=255)                                                                                                       cTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGAc                                                                                                                                                                        >  2:6684/1‑222 (MQ=255)                                                                                                       cTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGAc                                                                                                                                                                        <  1:6684/222‑1 (MQ=255)                                                                                                         gCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAa                                                                                                                                           <  1:188739‑M1/249‑2 (MQ=255)                                                                                                                    ggTATAGAACAACACACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTAAGAATCTCCTTAAGCCTCTATAAGATGTAGTGATAAAAATCAGTTTATTCTAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACNAGCTACAGAGACACAGAagcaatagttcatc                                                                                                                              >  2:229304‑M1/1‑237 (MQ=255)                                                                                                                       ataGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcag                                                                                                                            <  2:492298‑M1/250‑17 (MQ=255)                                                                                                                            acaacCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgcc                                                                                                                      >  1:263325‑M1/1‑229 (MQ=255)                                                                                                                                               aGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcagcagactgccgcagcagtagtatgatgat                                                                                                   <  2:44905‑M1/251‑42 (MQ=255)                                                                                                                                                   ccATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaat                                                                                               <  2:299033‑M1/251‑46 (MQ=255)                                                                                                                                                       tGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt                                                                                                                                                                                                                         <  2:315734/125‑1 (MQ=255)                                                                                                                                                       tGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt                                                                                                                                                                                                                         >  1:315734/1‑125 (MQ=255)                                                                                                                                                                        tcatcatcaTTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTCGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactatt                                                                          >  2:138098‑M1/1‑185 (MQ=255)                                                                                                                                                                                     aTTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacatt                                                              >  2:206156‑M1/1‑172 (MQ=255)                                                                                                                                                                                        aCCGTCTTGTTTGCAAGTGAGTCTTATTAGTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGAGTATTCCAGTGCGCCAACCAAATTAATTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtgctataactattatcggtaacattaacc                                                          <  2:61840‑M1/251‑83 (MQ=255)                                                                                                                                                                                               ggTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagca                                                    <  2:433259‑M1/250‑89 (MQ=255)                                                                                                                                                                                               ggTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGAGACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcag                                                   <  1:27733‑M1/251‑90 (MQ=255)                                                                                                                                                                                                           aGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGCTACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagaaggggcggtataac                                       >  1:498795‑M1/1‑150 (MQ=255)                                                                                                                                                                                                            gTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataac                                       >  1:400204‑M1/1‑149 (MQ=255)                                                                                                                                                                                                                    atttatATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataacactcaggct                              <  1:300776‑M1/251‑111 (MQ=255)                                                                                                                                                                                                                       tataTCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataacactcaggctcc                            <  1:279681‑M1/250‑113 (MQ=255)                                                                                                                                                                                                                       tataTCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataacactcaggctcc                            <  1:15236‑M1/250‑113 (MQ=255)                                                                                                                                                                                                                        atagcggtaTAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataacactcaggctccg                           <  1:58409‑M1/246‑114 (MQ=255)                                                                                                                                                                                                                                                 aCGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcaggggcggtataacactcaggctccgattgggctggaaggtaggtggaatt  <  1:315309‑M1/250‑139 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGATGCAGCCCAAAATTTACTGGATTGATAACCTGCGAGGGATAGCGTGTTTAATGGTGGTGATGATTCACACCACTACCTGGTATGTGACCAATGCTCATAGTGTTAGCCCCGTCACATGGGATATCGCCAATGTTCTGA  >  NC_000913/3725536‑3726025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑