| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,725,734 | G→A | intergenic (‑22/‑153) | ysaB ← / → wecH | putative lipoprotein YsaB/O‑acetyltransferase WecH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,725,734 | 0 | G | A | 97.4% | 137.2 / NA | 39 | intergenic (‑22/‑153) | ysaB/wecH | putative lipoprotein YsaB/O‑acetyltransferase WecH |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/22); minor base C (1/0); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGATGCAGCCCAAAATTTACTGGATTGATAACCTGCGAGGGATAGCGTGTTTAATGGTGGTGATGATTCACACCACTACCTGGTATGTGACC > NC_000913/3725506‑3725976 | tCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCg > 1:433259/1‑251 (MQ=255) gtctaTTTTTTGCTCGCCGGTGTTATAACGGTTTGCCGACGGAGCCTTGCATAACGCTTCCATATTCAACGAGCGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACTGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAAtct < 2:318549/247‑3 (MQ=255) gTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCTCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAAtct < 1:333758/251‑3 (MQ=255) gagtttttGCTCGCCGGTGTTATAACGTTTTGCCCCCTGATCCTTGCATAACGCTTCCATATTCACCGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCt < 2:325990/248‑1 (MQ=255) gCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCctc < 2:368080/251‑1 (MQ=255) cGGTGTTATAACGTCTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAg < 1:180716/251‑1 (MQ=255) tataACGTTTTGCCGCCTGATCCTTGCATATCGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTa < 1:195895/250‑1 (MQ=255) tataACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGt > 1:299033/1‑249 (MQ=255) aCGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt < 2:364598/251‑1 (MQ=255) gTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATa > 2:188739/1‑250 (MQ=255) ccgccTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGCTATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTc > 2:72324/1‑250 (MQ=255) cTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAATCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTcc > 2:259975/1‑250 (MQ=255) gCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTACAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGccaac < 2:166608/251‑1 (MQ=255) ttCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGTTATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAATGATAAAACTCAGATTATTCCCGTGCGccaacc > 2:27733/1‑250 (MQ=255) ttCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGccaacc > 1:308953/1‑250 (MQ=255) ccatagttaacgaacgCATAGGATCAACCTTTACTCGTTGTGGTTGCTGCACGGGGTACGGTATAGAACAACCAACTAGAACCATAAGCGACATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACTGTCTGGTTTGCAAGTGAGTCTTATTATGTATATCTGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCGATAAGAAGTAGTGATAACGCGCAGATTATTCCATTGCGcctaccaa < 2:418304/242‑1 (MQ=255) gaacgCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATNATTCCAGTGCGCCAACCAAATGATTTATCgg > 2:219383/1‑251 (MQ=255) gCAGAGTATCAACCTTTTCCCGTTGTTCGTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTGATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAg < 2:9986/251‑1 (MQ=255) cAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTCTAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCatc > 2:458652/1‑249 (MQ=255) aatcgttACCTGTTGTGCTTTATGCACGGGGTACGGTATAGAACAGCCCACTAGCATCATAAGCGCCCTTGCCGGAAAGAAAGTGTTCATCATCATATAATGACCGTCTTGTTTGCAAGTCAGTCTTATTATTTATATCGGTATAGTGGCTTATATCTTACGAATCTCCTTAAGCCTCTATAAGAGATAGTGATAAAACTCATATTATTCCAGTGCGCCAACCAAATCTTTTATCGGTCAGACCTTTTTTa < 2:157173/246‑1 (MQ=255) aCCCGTTGTGCTTTCTGCACTGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGAt < 2:8893/251‑1 (MQ=255) cccGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCATATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATa < 2:149276/251‑1 (MQ=255) ccGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATAc > 2:132426/1‑251 (MQ=255) gTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGAGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCACGTGAGTCTTATTATTTATATCGGTATAGAGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGCGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATAc > 1:218668/1‑249 (MQ=255) cTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGAc > 2:6684/1‑222 (MQ=255) cTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGAc < 1:6684/222‑1 (MQ=255) gCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAa < 1:188739‑M1/249‑2 (MQ=255) ggTATAGAACAACACACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTAAGAATCTCCTTAAGCCTCTATAAGATGTAGTGATAAAAATCAGTTTATTCTAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACNAGCTACAGAGACACAGAagcaatagttcatc > 2:229304‑M1/1‑237 (MQ=255) ataGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcag < 2:492298‑M1/250‑17 (MQ=255) acaacCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgcc > 1:263325‑M1/1‑229 (MQ=255) aGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcagcagactgccgcagcagtagtatgatgat < 2:44905‑M1/251‑42 (MQ=255) ccATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaat < 2:299033‑M1/251‑46 (MQ=255) tGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt > 1:315734/1‑125 (MQ=255) tGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGAt < 2:315734/125‑1 (MQ=255) tcatcatcaTTAAATTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTCGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactatt > 2:138098‑M1/1‑185 (MQ=255) aTTACCGTCTGGTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacatt > 2:206156‑M1/1‑172 (MQ=255) aCCGTCTTGTTTGCAAGTGAGTCTTATTAGTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGAGTATTCCAGTGCGCCAACCAAATTAATTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtgctataactattatcggtaacattaacc < 2:61840‑M1/251‑83 (MQ=255) ggTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagca < 2:433259‑M1/250‑89 (MQ=255) ggTTTGCAAGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATCTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGAGACAGAGACACAGAagcaatagttcatcagactgccgcagcagtagtatgatgatgaatcagggctgtactataactattatcggtaacattaaccgcagcag < 1:27733‑M1/251‑90 (MQ=255) | TCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCCAGTGCGCCAACCAAATGATTTATCGGTCAGACTTTTTTTAACCAGATACAGAGACACAGATGCAGCCCAAAATTTACTGGATTGATAACCTGCGAGGGATAGCGTGTTTAATGGTGGTGATGATTCACACCACTACCTGGTATGTGACC > NC_000913/3725506‑3725976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |