Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,226,228	T→C	intergenic (+57/‑6)	ebgC → / → ygjI	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta/putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,226,228	0	T	C	100.0%	165.8 / NA	46	intergenic (+57/‑6)	ebgC/ygjI	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta/putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/20);  total (26/20)

GTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCT  >  NC_000913/3225983‑3226472                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gTAGTGGAATATTAGCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGtt                                                                                                                                                                                                                                                 <  1:258063/251‑1 (MQ=255)           attatCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatcct                                                                                                                                                                                                                                       >  1:350634‑M1/1‑241 (MQ=255)           attatCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatcc                                                                                                                                                                                                                                        <  1:313845‑M1/250‑10 (MQ=255)                       aaaCTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatgg                                                                                                                                                                                                                           >  2:201409‑M1/1‑229 (MQ=255)                            gACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaa                                                                                                                                                                                                                        >  1:16156‑M1/1‑224 (MQ=255)                                          aaaaGGTTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATTTCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttc                                                                                                                                                                                                         <  1:74725‑M1/250‑41 (MQ=255)                                            aaGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaaaggcgcttcac                                                                                                                                                                                                       >  1:465828‑M1/1‑208 (MQ=255)                                            aaGGCTGCGGAGAAAACGATGAGGTCCACGCCGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAACGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGCGACGCGTTgattaatccttagccgggatggacaagcaaacggcgcttcac                                                                                                                                                                                                       >  2:189198‑M1/1‑208 (MQ=255)                                                    ggcgaaacCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCCACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaa                                                                                                                                                                                               >  1:200942‑M1/4‑200 (MQ=255)                                                    ggAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaa                                                                                                                                                                                               >  1:200950‑M1/1‑200 (MQ=255)                                                       gaAACCGTTGATGTCCTCGAAGGGCAAATCGGTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAATTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTGTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcgcattatgaaacgc                                                                                                                                                                                           <  2:313707‑M1/251‑55 (MQ=255)                                                                       aCGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaaca                                                                                                                                                                            >  1:34379‑M1/1‑181 (MQ=255)                                                                               cAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacatctcatttc                                                                                                                                                                   <  1:193635‑M1/251‑79 (MQ=255)                                                                               cAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttc                                                                                                                                                                   <  1:490281‑M1/251‑79 (MQ=255)                                                                                                   ccATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCCAAAGGAGTTTGCCGCCACCGCTTCCCTACTCATTTTCGGAGCCGTGTTgataaatccttagcaggtatggacaagcaaacggcgcttcacattatgaaacgccgttttttattaacacctcatttcgactttatagcgttacgccg                                                                                                                                               <  1:479633‑M1/251‑99 (MQ=255)                                                                                                   ccATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatgggaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccg                                                                                                                                               >  2:382006‑M1/1‑153 (MQ=255)                                                                                                     aTGAATCGTATGGGTTTATCTGCAATAGCGCGGTAAAAAAAGGGGTTCTCAAAGTCACCAACGAAGATGGTTATTTCCATAAAAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggctcttcacattatgaaacgccgttttttataaacaactcatttcgactttatagcgttacgccgct                                                                                                                                             <  2:282651‑M1/251‑101 (MQ=255)                                                                                                      tGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgctt                                                                                                                                            >  2:485406‑M1/1‑150 (MQ=255)                                                                                                          gCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttga                                                                                                                                        <  2:311472‑M1/251‑106 (MQ=255)                                                                                                              aTCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttga                                                                                                                                        >  1:304510‑M1/1‑142 (MQ=255)                                                                                                                     tATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcagggatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatctccagt                                                                                                                             >  2:404986‑M1/1‑135 (MQ=255)                                                                                                                      aTCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagccggtatggaaaagcaaacggcgcttcccattatgaaaagccgttttttattaccaacccatttcgactttatagcggtacgcctcttttgacgatctcccgtt                                                                                                                            >  2:32440‑M1/1‑134 (MQ=255)                                                                                                                          gCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaaatcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcg                                                                                                                        >  1:392962‑M1/1‑130 (MQ=255)                                                                                                                                 cgcgGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcg                                                                                                                 >  1:234065‑M1/1‑123 (MQ=255)                                                                                                                                         aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctc                                                                                                         <  1:312724‑M1/251‑137 (MQ=255)                                                                                                                                              gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttac                                                                                                    >  2:108783‑M1/1‑110 (MQ=255)                                                                                                                                                      cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggttt                                                                                              <  1:108783‑M1/249‑148 (MQ=255)                                                                                                                                                                aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttgg                                                                                  <  1:480804‑M1/251‑160 (MQ=255)                                                                                                                                                                  cGAAGTTGGTTATTTCCATAACAAATAACAANTTCGGCGGCAAAAGGAGTTTGCCGCCACCGCTTCCCTACTCAGTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaactgcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacagggtttcttaccgttggca                                                                                <  2:225910‑M1/251‑162 (MQ=255)                                                                                                                                                                  cGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggc                                                                                 >  1:137319‑M1/1‑90 (MQ=255)                                                                                                                                                                   gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCCCCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcacacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagctcgccagttcgcggaagtcgttaagctcgttacagggttgcttaccgttggca                                                                                >  2:399997‑M1/1‑89 (MQ=255)                                                                                                                                                                   gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagccaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggca                                                                                >  1:147981‑M1/1‑89 (MQ=255)                                                                                                                                                                   gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggca                                                                                >  1:390590‑M1/1‑89 (MQ=255)                                                                                                                                                                   gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacatt                                                                                                                                                                                                    <  1:126936‑M1/134‑46 (MQ=255)                                                                                                                                                                   gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACACTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacctt                                                                                                                                                                                                    >  2:126936‑M1/1‑89 (MQ=255)                                                                                                                                                                             aTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTCTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgat                                                                     >  1:321293‑M1/1‑79 (MQ=255)                                                                                                                                                                                     aacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgacgttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcg                                                              <  2:455368‑M1/250‑180 (MQ=255)                                                                                                                                                                                        aaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgata                                                           >  1:247174‑M1/1‑68 (MQ=255)                                                                                                                                                                                               aacTACGGCGGCAAAAGGAGTTTGCCGCCAACGCTACCCTACTAATTTTCGGGGACGCGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgacacggcgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggacgtcgttacgctcgttaccggtttgcttaccgttggcgaactcaaagatgggggcgataaattcttc                                                   >  2:298577‑M1/1‑61 (MQ=255)                                                                                                                                                                                                          cAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccg                                                                                      <  2:231880‑M1/205‑156 (MQ=255)                                                                                                                                                                                                          cAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccg                                                                                      >  1:231880‑M1/1‑50 (MQ=255)                                                                                                                                                                                                                    ttGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgataaattcttccagcaactgcggcatcgctt                               <  1:125405‑M1/250‑211 (MQ=255)                                                                                                                                                                                                                         gccACCGCTACCCTACTCATTTTCGGAGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgataaattcttccagcaactgcggcatcgctttaccgt                         <  1:201409‑M1/251‑217 (MQ=255)                                                                                                                                                                                                                                          cATTTTCGGAGACGTGTTgataaatccttancaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgataaattcttccagcaactgcggcatcgctttaccgtggatcaactgacccgc         <  2:247174‑M1/250‑233 (MQ=255)                                                                                                                                                                                                                                              ttCGGCGACGTGTTgataaatccttagcaggtatggaaaagcaaacggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgataacttcttccagcaactgcggcatcgctttaccgtggatcaactgacccgcgtca     >  2:130219‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                                                cGGAGACGTGTTgataaatccttagcaggtatggaaaagcaatcggcgcttcacattatgaaacgccgttttttattaacaactcatttcgactttatagcgttacgccgcttttgaagatcgccagttcgcggaagtcgttacgctcgttacaggtttgcttaccgttggcaaactcaacgatggtgtcgataaattcttccagcaactgcggcatcgctttaccgtggatcaactgacccgcgtcaaag  <  2:352312‑M1/251‑240 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATATCCATGAAGCGTATCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGTTATGTCTGATACCAAACGTAATACAATCGGCAAATTCGGCTTGCTCTCGCTGACTTTTGCCGCCGTTTACAGCTTTAACAACGTTATCAATAATAATATTGAGCTTGGACTGGCCTCGGCACCGATGTTTTTCCTCGCGACGATTTTTTATTTTATTCCCTTCTGTCTGATCATCGCAGAATTTGTTTCGTTAAATAAAAACTCAGAAGCCGGTGTCTACGCGTGGGTAAAAAGTTCGCT  >  NC_000913/3225983‑3226472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑