Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,190,725	A→G	N32D (AAT→GAT)	yqiI →	putative fimbrial protein YqiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,190,725	0	A	G	100.0%	125.3 / NA	34	N32D (AAT→GAT)	yqiI	putative fimbrial protein YqiI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/22);  total (12/22)

CAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTG  >  NC_000913/3190491‑3190965                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 cAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTgg                                                                                                                                                                                                                                  >  2:155716/1‑251 (MQ=255)              tAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAg                                                                                                                                                                                                                      <  2:83144/250‑1 (MQ=255)               aaaaaTGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGCAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGa                                                                                                                                                                                                                     <  1:432496/250‑1 (MQ=255)                  aaTGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAtct                                                                                                                                                                                                                 <  1:369049/251‑1 (MQ=255)                        ccGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATcc                                                                                                                                                                                                           >  1:147758/1‑251 (MQ=255)                                                    aTGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTCAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATCAAAt                                                                                                                                                                               >  2:371482/1‑251 (MQ=255)                                                               ggATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCa                                                                                                                                                                    <  2:147758/251‑1 (MQ=255)                                                                            aTGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGTGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGTGTTTTAGTTCATTACCTGCATGGGAAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGGCATAGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAATGAATATTTTa                                                                                                                                                        <  2:408678/250‑1 (MQ=255)                                                                              gAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATg                                                                                                                                                      <  2:314465/250‑1 (MQ=255)                                                                                    aaCTGCCCTGTAGTAAATAACATTGGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGttt                                                                                                                                               <  1:327061/251‑1 (MQ=255)                                                                                       tGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCt                                                                                                                                            >  1:198833/1‑251 (MQ=255)                                                                                        gCCCTTTAATAAATAAAATGTGTAATATTTCTGTAGCCATCCGAGAACAAGAATATGCGATACATGTTAACTGTTATTACTTTTTTTATGGGTTTTAATTCATTACCTGCATGGGCAATGGACTGTTATGCTGAACATGAAGGTGGAGATACCGGTGTCATTGGTTACGTACCAAGAATCTCTAGCCCAAGCGATGGTAAAAAAGGTGATAAAATCTGGCAACGCAGTGAATAATTTATGAAGGTTGTCt                                                                                                                                            <  2:23330/250‑1 (MQ=255)                                                                                        gCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTg                                                                                                                                           <  2:372762/251‑1 (MQ=255)                                                                                         cccTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGt                                                                                                                                          <  1:371454/251‑1 (MQ=255)                                                                                               agtaAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaataa                                                                                                                                     <  1:112300/250‑1 (MQ=255)                                                                                                gtaAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTTCGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTGTCTGTAATAATg                                                                                                                                   <  1:351382/251‑1 (MQ=255)                                                                                                        cATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTAcc                                                                                                                            >  2:88383/1‑250 (MQ=255)                                                                                                         aTTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTCCCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTAcc                                                                                                                            >  1:323417/1‑249 (MQ=255)                                                                                                                    ttCTGGAGGCAACCGAGATCAATAATATGCTCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCTCCAGCGATGGTAAAAAAGTTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCGCATCTcc                                                                                                                <  2:33493/250‑1 (MQ=255)                                                                                                                        gTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGgag                                                                                                           <  1:40497/251‑1 (MQ=255)                                                                                                                                ccGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATAc                                                                                                   >  1:219750/1‑251 (MQ=255)                                                                                                                                        aataatATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAACAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGc                                                                                           >  1:287868/1‑251 (MQ=255)                                                                                                                                                          ttAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAatgat                                                                         >  1:318361/1‑251 (MQ=255)                                                                                                                                                                  tatttCTTTTTTGATGGTTTTTAGTGAATTTCCTGTATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATTTTTTCTGTAATAATGCACTACTAGGACCATCTCCAGGAGAAGAATACCCATCTGCATGGTCAAATATAATGATGTTGTTa                                                                  <  2:345347/250‑1 (MQ=255)                                                                                                                                                                      aCTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTCACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGc                                                                                                                                  <  2:101513/182‑1 (MQ=255)                                                                                                                                                                      aCTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTCACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGc                                                                                                                                  >  1:101513/1‑182 (MQ=255)                                                                                                                                                                       cTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATc                                                             >  1:51865/1‑250 (MQ=255)                                                                                                                                                                            tttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTc                                                        <  1:381102/250‑1 (MQ=255)                                                                                                                                                                              tATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCaa                                                      <  1:165030/250‑1 (MQ=255)                                                                                                                                                                                            cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTCCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCaa                                       <  2:457111/251‑1 (MQ=255)                                                                                                                                                                                            cATTAACTGCATGGGCAATGGAATGCTATGCTGAAAATGAAGGTGGAGATACCGTTGTCATAGGATAAGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCCTCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCa                                        >  1:419418/1‑250 (MQ=255)                                                                                                                                                                                                     cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTtat                              <  1:280813/251‑1 (MQ=255)                                                                                                                                                                                                                       aTGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTta             <  1:258350/250‑1 (MQ=255)                                                                                                                                                                                                                                  gAAGGTGGAGATACCGTTGTCATTGGTTACGTAACAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTg  <  2:351975/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 CAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTATGAAATAAACTGCCCTGTAGTAAATAACATTTGTAATATTTCTGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTG  >  NC_000913/3190491‑3190965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑