Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,089,216	Δ255 bp		[yoeB]–[yefM]	[yoeB], [yefM]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2089216	2089470	255	52 [0]	[1] 51	[yoeB]–[yefM]	[yoeB],[yefM]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2089215	0 (0.000)	50 (0.780)	46/468	0.1	100%	coding (251/255 nt)	yoeB	ribosome‑dependent mRNA interferase toxin YoeB
	?	NC_000913	2089471 =	0 (0.000)					coding (243/252 nt)	yefM	YefM antitoxin of the YoeB‑YefM toxin‑antitoxin pair and DNA binding transcriptional repressor

CATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCTTATCTGTTTCCTGCCAGTACAGATAATCGTCCCATGATTCCTCAGACCAGATTAGTTTCACTCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTC  >  NC_000913/2089227‑2089716                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccatgcgaagcagatagctatcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTc                                                                                                                                                                                                                                                  <  1:231405‑M2/6‑1 (MQ=255)       gaagcagatagctatcaatgccactcatgcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTcc                                                                                                                                                                                                                                           <  2:211604‑M2/13‑1 (MQ=255)         agcagatagctatcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGt                                                                                                                                                                                                                                         <  1:81253‑M2/15‑1 (MQ=255)         agcagatagctatcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGt                                                                                                                                                                                                                                         >  2:349847‑M2/237‑251 (MQ=255)              attgctaacaattgctctcattcgtgccgcttcgacaccagcttgtgtgtttttactcccggtgtcttgccagcctcgcgctgaaagtgacatctccagcggcatgcctaacccccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctattaatgaatctgtgcaatGACGTCCTTTTCCGTTCCtt                                                                                                                                                                                                                                    <  1:156364‑M2/20‑1 (MQ=255)                    atcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGcc                                                                                                                                                                                                                                <  2:69832‑M2/24‑1 (MQ=255)                    atcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGcc                                                                                                                                                                                                                                <  1:453277‑M2/24‑1 (MQ=255)                    atcaatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTg                                                                                                                                                                                                                              >  1:48860‑M2/226‑251 (MQ=255)                      ccatgccactcagtcgggccgattcgacaccatcctgtggggtttttctcccggttacttgccagcctcgcgctgaaagtgacatcgccatctgcatgcctatccaccctaacccgacaattgcgccctgtttcatccggtatctcctgatacaccttagatctataatgctacgctagcgtatcaaaactgtcaattcattctatgaatgaatctgttcaatGACGTCCTTATCCGTTCCTTTGCCTGa                                                                                                                                                                                                                             <  2:290692‑M2/27‑1 (MQ=255)                      caatgccactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgccaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTgccttat                                                                                                                                                                                                                            >  2:456330‑M2/224‑248 (MQ=255)                            cactcattcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAgg                                                                                                                                                                                                                      >  1:175887‑M2/218‑251 (MQ=255)                                   tcgggccgcttcgacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCg                                                                                                                                                                                                                <  2:314577‑M2/40‑1 (MQ=255)                                               gacaccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATc                                                                                                                                                                                                    >  1:413539‑M2/199‑250 (MQ=255)                                                   ccttcttgtgttgttttactcccgggtacttgccagcctcgagctgaaagtgacagcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataagtctacgctagcgtatcaatactgacaattcattctatgaatgaatctgttcaatTACGTCCTTTTCCGTGCCTTTTCCTGATTTCAGGCTATCGATTAAGTCCATCAatcg                                                                                                                                                                                               <  2:479746‑M2/57‑2 (MQ=255)                                                   ccatcttgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtct                                                                                                                                                                                               <  2:389537‑M2/57‑1 (MQ=255)                                                         tgtgtggttttactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCg                                                                                                                                                                                            <  1:60322‑M2/60‑1 (MQ=255)                                                                   tactcccgtggacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCggg                                                                                                                                                                                <  2:842‑M2/72‑1 (MQ=255)                                                                   tactcccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg                                                                                                                                                                               >  1:102689‑M2/179‑251 (MQ=255)                                                                        ccggtgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGGTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAgcg                                                                                                                                                                          <  1:412151‑M2/78‑1 (MQ=255)                                                                            tgacttgccagcctcgcgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGt                                                                                                                                                                      >  2:364507‑M2/170‑251 (MQ=255)                                                                              acttgccagcctcgcgctgaaagtgacatcgccggcggcatgcctaaccaccctaaaccgacaattgcgacctttttcatccgttatctcctgagacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgactgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGt                                                                                                                                                                      >  2:280774‑M2/168‑249 (MQ=255)                                                                                     agcctcgcgctgaaagtgacatcgccagcggnatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGcc                                                                                                                                                             <  2:228690‑M2/91‑1 (MQ=255)                                                                                          cgcgctgaaagtgacatcgccagcggcatgcctaacctccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGtct                                                                                                                                                         <  1:69842‑M2/95‑1 (MQ=255)                                                                                          cgcgctgaaagtgacatcgccagcggcatgcctaacctccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGGCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGtctc                                                                                                                                                        <  1:289953‑M2/96‑1 (MQ=255)                                                                                            cgctgaaagtgacatcgccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCtt                                                                                                                                                      <  1:226496‑M2/98‑1 (MQ=255)                                                                                                            gccagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTAttc                                                                                                                                      >  2:31977‑M2/138‑251 (MQ=255)                                                                                                              cagcggcatgcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTAttctt                                                                                                                                    <  2:170619‑M2/116‑1 (MQ=255)                                                                                                               agcggcatgcctaaccaccttaacccgacaattgcgccctttttcatccgttatctccttataccccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctggtcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGTGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGCGTTGTAttcttc                                                                                                                                   <  2:96254‑M2/117‑1 (MQ=255)                                                                                                                       gcctaaccaccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGAc                                                                                                                            <  1:168237‑M2/124‑1 (MQ=255)                                                                                                                              caccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAac                                                                                                                    >  1:154057‑M2/120‑251 (MQ=255)                                                                                                                                ccctaacccgacaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAacac                                                                                                                  <  1:31977‑M2/134‑1 (MQ=255)                                                                                                                                          acaattgcgacctttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTcc                                                                                                        >  2:132107‑M2/108‑251 (MQ=255)                                                                                                                                                   cagacgtgtgctctccgatctctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTctggc                                                                                                <  1:83371‑M2/152‑3 (MQ=255)                                                                                                                                                      tttttcatccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCcattctggcgtgt                                                                                            >  2:377668‑M2/96‑245 (MQ=255)                                                                                                                                                              ccgttatctcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGCGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg                                                                                     >  2:264719‑M2/88‑250 (MQ=255)                                                                                                                                                              ccgttatcgcctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg                                                                                     <  1:364507‑M2/163‑1 (MQ=255)                                                                                                                                                                       cctgatacaccttagatcaataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCggg                                                                               <  2:16567‑M2/169‑1 (MQ=255)                                                                                                                                                                        ctgatacaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTctggc                                                                                                >  2:83371‑M2/78‑227 (MQ=255)                                                                                                                                                                             acaccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCtt                                                                     >  1:198655‑M2/73‑251 (MQ=255)                                                                                                                                                                              caccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc                                                                    >  2:15001‑M2/72‑251 (MQ=255)                                                                                                                                                                               accttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc                                                                    >  1:137897‑M2/71‑250 (MQ=255)                                                                                                                                                                                ccttagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCa                                                                   >  2:146690‑M2/70‑250 (MQ=255)                                                                                                                                                                                   tagatctataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAAc                                                                 <  1:146690‑M2/183‑1 (MQ=255)                                                                                                                                                                                        gtataaggctacgctagcgtatcaaaactgacaaatcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTATTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGGAANAAGGAACGGGGCATGCTCTTTAACGGCTTtc                                                          >  2:238552‑M2/62‑251 (MQ=255)                                                                                                                                                                                          ataagtcaacgtaagcgtatcaaaactgaaaattcattctatgatgtaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGGTTTGGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGCCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCttt                                                           <  2:324159‑M2/189‑1 (MQ=255)                                                                                                                                                                                          ataaggctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat                                                        >  2:460738‑M2/60‑251 (MQ=255)                                                                                                                                                                                               gctacgctagcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg                                                   >  2:3217‑M2/55‑251 (MQ=255)                                                                                                                                                                                                       agcgtatcaaaactgacaattcattctatgaatgaatctgttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGAc                                           <  1:56805‑M2/205‑1 (MQ=255)                                                                                                                                                                                                                                    gaatgaatctgttcaatAACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGGTATCGATTGAGTCCATCAATCGCCGGGCGGTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAAt              <  1:491678‑M2/234‑1 (MQ=255)                                                                                                                                                                                                                                               ttcaatGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGt   >  1:456227‑M2/7‑251 (MQ=255)                                                                                                                                                                                                                                                 cAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTc  >  1:396167/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CATGCTGCAATGAGCAGTGAATCGTCGGTAACCGCGTATACCAGACGGTGCTCCTCTGTAATGCGTCGGGACCAGAAACCTGACAAATTATGTTTCAGGGGTTCTGGCTTCCCCTTACCTTCAAATGGCGTTCTGCGGGTATCTTTGATAAGTTCATTGATCTTTTTAACAATTCGCTTATCTGTTTCCTGCCAGTACAGATAATCGTCCCATGATTCCTCAGACCAGATTAGTTTCACTCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTC  >  NC_000913/2089227‑2089716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑