BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	98.3%	435.2 / NA	119	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (55/60); total (57/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674275‑674762

tgtttgttCGCCAGTGCGGTGTGGGCGTTCGGGCACCAGTGTACCGCAGAAGTTTGTTTATATACCAGGCCTTTTTTATACAGCTGGGTGAAGAATTTTTGTTCCCAACGGTAGTATTCCGGAGTTCAGGTTGCCAGCTCGCGGCTCCAGTCAAAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGGGAT‑TTTGT‑CGTACGCCCACGGTGC‑AGGAGCGGTGTT‑GTTTTTCTCC‑GCCACGCCtt                                                                                                                                                                                                                                                <  2:745144/250‑1 (MQ=255)

tGTTCGTTCGCCAGTGCGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCACCGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGGGCGGGTTTTTCATATCCGCGAT‑GTTGT‑CGTACGTCCCCGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCtt                                                                                                                                                                                                                                                <  2:1188417/250‑1 (MQ=255)

     gttcgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGGGGTTTT‑GTTTTTCACC‑GCCACGCCTTCCgcag                                                                                                                                                                                                                                          <  1:240094/251‑1 (MQ=255)

        cgccgtgaCGGTCGGGCCGTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCCCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGGGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCgcaggca                                                                                                                                                                                                                                       <  2:510044/244‑1 (MQ=255)

         cccAGTACGGTCGGGTCGTTCGGGCACCAGTTGACCGCAGAAGTTTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCAGAACCAAAG‑CCCAGCATTT‑TGAGCGGGTTTTTCATATACGCGAT‑GTTGT‑CTTACGCCCACGGTGC‑CGGAGGGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCgcaggcag                                                                                                                                                                                                                                      <  1:1062476/250‑1 (MQ=255)

           cAGTACGGTCTGGTCGTTCGGGCCCCATTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGAc                                                                                                                                                                                                                                    <  2:593529/251‑1 (MQ=255)

                cGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGAAGGCAGACCAAAc                                                                                                                                                                                                                               >  2:704577/1‑251 (MQ=255)

                 ggtctggtctTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTCCGTCCCCGTTGC‑CGGAGCGGTTTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAAcg                                                                                                                                                                                                                              <  1:1035688/251‑1 (MQ=255)

                 ggTCTGGTCGTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATCTCCCAGGCCTTTTTTATACAGCCCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTCCAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATCCGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAAcg                                                                                                                                                                                                                              <  2:1068677/251‑1 (MQ=255)

                                 cACCAGTTGACCGCAGAAGTCTTCTTATATGCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAAGGGTAGTATTCCGGTGTACGGGTTGCCAGCTCGCGGCTCCAGTCATACCCAAAG‑CCCGGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTTT‑TGTACGTCCACGGTCC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCgg                                                                                                                                                                                                              <  1:129281/251‑1 (MQ=255)

                                  cccAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc                                                                                                                                                                                                             <  1:314122/250‑1 (MQ=255)

                                           ccGCAGAAGTCTTCTTATATCCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACg                                                                                                                                                                                                    <  1:410457/251‑1 (MQ=255)

                                                aaattgttttgagataCCTGGCCTTTTTTATAGAGCTCGGTGAAGAATTTCTTTCCCCAGCGGTAGTATTCCGGCTTACAGGTTGCCAGTGTGGGGCTCCAGTGATAACAAAAG‑CCCAGCATTT‑TGAGGTGGTTTTACATATACGCGAT‑GTTGT‑CTTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGttttt                                                                                                                                                                                               <  2:822193/238‑1 (MQ=255)

                                                     cttcttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                           >  1:723537/1‑250 (MQ=255)

                                                       tattATTTACCAGTCCTTTTTTACACAGCGCGGTGAAGAATTTCTGTCCCCAACGGTAGTTTTCCGGCTTACAGGTTGCCCGCGCGCGGCTCCAGTCATAACCAAAG‑CCAAGCATTT‑TGAGGTGGTTTTTTATATCCGCGTG‑GTTGT‑CGGCCGCCCACGGTGC‑CGGAGCGGTGTT‑GTTTTCCTCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTGTTGCCCAg                                                                                                                                                                                         <  2:627361/248‑1 (MQ=255)

                                                         ttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCAt                                                                                                                                                                                      >  2:410654/1‑251 (MQ=255)

                                                         ttATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCAt                                                                                                                                                                                      >  1:455106/1‑251 (MQ=255)

                                                           atatcCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGa                                                                                                                                                                                                                  <  1:712794/221‑1 (MQ=255)

                                                           atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGa                                                                                                                                                                                                                  >  2:712794/1‑221 (MQ=255)

                                                           atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAc                                                                                                                                                                                    >  2:36996/1‑251 (MQ=255)

                                                              taCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCt                                                                                                                                                                                 >  1:182198/1‑251 (MQ=255)

                                                              taCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCt                                                                                                                                                                                 >  1:270096/1‑251 (MQ=255)

                                                               aCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTg                                                                                                                                                                                >  2:1017004/1‑251 (MQ=255)

                                                                   gcctttttttATACAGCGCGGTAAAAAATTTTTGTCCCAAACGGTATTATTCGGGCGTACGGGTGGCCAGCTCGCGGCCCCAGTCAAAACAAAGG‑CCCAGCTTTT‑GGAGCTGGTTTTTAATATACGCGAT‑GTGGT‑CGTACGTCCACGGTGC‑CGGAGGGGTGTT‑GTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAg                                                                                                                                                                            <  2:343512/247‑1 (MQ=255)

                                                                       ttttttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                                                                                                                        >  2:1156431/1‑251 (MQ=255)

                                                                           ttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                    >  1:564686/1‑251 (MQ=255)

                                                                            tataCAGCCCGGTGAAGAATTTCGGTCCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCCCGCGGCTCCGGTCATAACCAAAG‑CCCAGCATTT‑TGGGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTTTT‑TTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAt                                                                                                                                                                    <  2:691147/250‑1 (MQ=255)

                                                                                 aGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTCCAGGTTGCCAGCTCGCGGCTCCAGTCATACCCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                              <  1:166798/251‑1 (MQ=255)

                                                                                         gaagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAACG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGCGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAACCGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                                                                                                                      >  2:858965/1‑251 (MQ=255)

                                                                                         gaagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                                                                                                                      >  2:507318/1‑251 (MQ=255)

                                                                                         gaagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATgg                                                                                                                                                      >  1:203576/1‑251 (MQ=255)

                                                                                                    tgtCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtac                                                                                                                                             <  1:913976/247‑1 (MQ=255)

                                                                                                     ttCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgt                                                                                                                                           >  2:983534/1‑250 (MQ=255)

                                                                                                       cccAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacg                                                                                                                                        >  2:445141/1‑251 (MQ=255)

                                                                                                       cccAACGGTAGTATTCAGGCGTACAGGTGCCCTGCGCCGGGCCACGGTCATAACAAAGG‑CCCCG‑ATTTGTGATCAGGTTTTTCATAACCGCGAT‑TTGGC‑CGTGCGACCGCGTTCC‑CGGAGGGGTGTT‑GTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacg                                                                                                                                        <  2:171668/251‑1 (MQ=255)

                                                                                                          aaCGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTgg                                                                                                                                     >  1:466487/1‑251 (MQ=255)

                                                                                                             ggTAGTATTCCGGCGTACAGGTTGCCCGCTCGCGGCTACAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCCCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGCCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTCGTTACGACCGTGGccc                                                                                                                                  >  2:312152/1‑251 (MQ=255)

                                                                                                             ggTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGccc                                                                                                                                  >  2:161308/1‑251 (MQ=255)

                                                                                                               tagtaTTCCGGCTTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCTTTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGCCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgt                                                                                                                                                     <  1:862186/230‑1 (MQ=255)

                                                                                                               tagtaTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgt                                                                                                                                                     >  2:862186/1‑230 (MQ=255)

                                                                                                                    ttCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATgtgt                                                                                                                            <  1:36996/250‑1 (MQ=255)

                                                                                                                      ccGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGt                                                                                                                         >  1:858783/1‑251 (MQ=255)

                                                                                                                       gggCGTCCAGGTTGCCAGCCCGCGGCTCCAGCCATAACCAAAC‑CCCAGCTTTT‑TGAGCTGGTTTTTCATATGCGCGAT‑GTTGT‑CGTACGCCCACGGTGC‑GGGAGGGGTGTT‑GTTTTTCGCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                        <  2:746529/250‑1 (MQ=255)

                                                                                                                       cGGCGTACAGTTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGTGGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                        <  1:704577/251‑1 (MQ=255)

                                                                                                                       cGGCGTACAGGTTGCCGTCCCGCGGTCCCAGT‑AAAACCAAAGGCCCAGCATTT‑GGGGCTGTTTTTTAATATACGCGA‑‑GTTGTTCGTCCGTCCACGGGGC‑CGGAGGGGTGTT‑GTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                        <  2:936638/251‑1 (MQ=255)

                                                                                                                       cGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCAAAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGGGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                        <  1:519931/251‑1 (MQ=255)

                                                                                                                         gCGTACAGGTGGCCACCTCGCGGCCCCAGTCATACCCAAGG‑CCCAGCATTT‑GGAGCGGGTTTTTCATATACGCGAT‑GTTGC‑CGTCCGCCCACGGGGC‑CGGAGCGGTGTT‑GTTTTCCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGa                                                                                                                      <  2:401028/251‑1 (MQ=255)

                                                                                                                           gTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                    >  2:495053/1‑251 (MQ=255)

                                                                                                                           gTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                    >  1:639825/1‑251 (MQ=255)

                                                                                                                           gTACAGGTTGCCAGCCCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCCCCGATGGCGTAGTTACGTACGTCGCCCATGTGTAGTCGAcc                                                                                                                    >  2:23133/1‑251 (MQ=255)

                                                                                                                              cAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGGGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                   <  1:766702/201‑1 (MQ=255)

                                                                                                                              cAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                   >  2:766702/1‑201 (MQ=255)

                                                                                                                              cAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                    >  1:947334/1‑248 (MQ=255)

                                                                                                                               aGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa                                                                                                                 >  2:860990/1‑250 (MQ=255)

                                                                                                                               aGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGa                                                                                                                 >  1:960753/1‑250 (MQ=255)

                                                                                                                                 gTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg                                                                                                              >  1:879005/1‑251 (MQ=255)

                                                                                                                                  ttgccgcctcgcgtatCCAATCAAAACCAAAG‑CCCAGCATTT‑TGTGCTGTTTTTTCATATACGCGAT‑GTTGT‑GGTCCGTCCACGTTGC‑CGGGGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAgg                                                                                                              <  2:717088/235‑1 (MQ=255)

                                                                                                                                        gcccgcgGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGa                                                                                                       <  1:574368/248‑1 (MQ=255)

                                                                                                                                         cccgcgTCTCCAGCAATACCCAACG‑CCCAGCTTTT‑TGAGCTGGTTGTTCATATCCGCGAT‑GTTGT‑GTTCCGTCCAGGGTCC‑CGG‑GGGGTGTT‑TTTTTTCCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGaa                                                                                                      <  2:687810/249‑1 (MQ=255)

                                                                                                                                             gggccccggTCATACCAAAAG‑CCCAGCATTT‑TGTGCGTGTTTTCCATTTTCGGGATTGTT‑T‑CGGACTTCCACGGGTC‑GGGAGGGGGGTT‑GTTTTACACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                                  <  2:1098560/243‑1 (MQ=255)

                                                                                                                                              ggccccAGTCAAACCCAAAG‑CCCAGCTTTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTGGT‑CGTCCGTCCGCGGTGC‑CGGAGCGGTTTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATa                                                                                                 <  2:704500/247‑1 (MQ=255)

                                                                                                                                                 cccagccaaaaCCAAGG‑CCCGTCATTT‑TGGGCTGTTTTTTCATATACGCGAT‑GTTGT‑CGTACGCCCACGGTGC‑GGGAGCGGTGTT‑GTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGAc                                                                                              <  2:947334/242‑1 (MQ=255)

                                                                                                                                                   cAGTCATAACCAAAC‑CCCAGCATTT‑TGGGCTGGTTTTTCATATACGCGAG‑GTTGT‑CGTACGTCCACGGTGC‑CGGGGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAg                                                                                            <  2:23681/251‑1 (MQ=255)

                                                                                                                                                    aGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAgg                                                                                           >  1:506414/1‑251 (MQ=255)

                                                                                                                                                     gTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGCATGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTTTAGTCGACCAGAAGGATAGGGATGCATAGACAggg                                                                                          >  2:89399/1‑250 (MQ=255)

                                                                                                                                                        atacccAAAG‑CCCACCATTT‑TGGGTGGGTTTGTCATAAACGCGAT‑GTTGT‑CGACCGCCCTGGGGGC‑GGGGGCGGGTTT‑GTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGt                                                                                       <  2:270096/247‑1 (MQ=255)

                                                                                                                                                             caagg‑CCCAGCATTT‑TGAGCTGGTTTTTCATATCCGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATAc                                                                                  <  1:79954/247‑1 (MQ=255)

                                                                                                                                                               aaG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTa                                                                                                                                                                             >  1:866183/1‑158 (MQ=255)

                                                                                                                                                               aaG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACtt                                                                                >  2:106572/1‑251 (MQ=255)

                                                                                                                                                                     cAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCtt                                                                           >  1:868108/1‑251 (MQ=255)

                                                                                                                                                                      aGCATTT‑TGAGCTGGTTTTTCATATGCGCGAT‑TTTGT‑CGTACGTCCACGGTGC‑CGGGGGGGTGTT‑TTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCttt                                                                          <  1:1116941/251‑1 (MQ=255)

                                                                                                                                                                               gAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGt                                                                  >  1:866925/1‑251 (MQ=255)

                                                                                                                                                                                aGCTGGTTTTTCATATCCGCGAT‑GTTGT‑CGTCCGTCCACGGTGC‑CGGAGCGGTGTT‑TTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTc                                                                 <  1:1017004/251‑1 (MQ=255)

                                                                                                                                                                                 gCTGGTTTTTCATATACGTGAT‑GTTGT‑CATACGTCCACGGTGC‑CGGAGCGGTGTT‑GGTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGGCGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGGTGGTGTAGTTACGTTCGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGGAGCATGGACAGGCAGATATACTTCTCTTTTCTCTCGTCt                                                                >  2:610364/1‑251 (MQ=255)

                                                                                                                                                                                  cgggTTTTTCATATCCGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGTAGCGGTGTT‑GTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCtt                                                               <  1:627163/249‑1 (MQ=255)

                                                                                                                                                                                   tGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTATTTGCTCTCGTCTTc                                                              >  2:427677/1‑251 (MQ=255)

                                                                                                                                                                                    ggTTTTTCATATGCGGGTT‑GGTGG‑CGTACGCCCACGGTGC‑GGGGGCGTTGTT‑TTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCg                                                             <  2:1108606/251‑1 (MQ=255)

                                                                                                                                                                                       ttttCATATACGCGCT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGCTGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGaa                                                                                                      >  2:1048226/1‑207 (MQ=255)

                                                                                                                                                                                          ttcatgtgcgggagtttgt‑CGTACGCCCA‑GGGGCGCGGGGCGGTGTT‑GTTTTACACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCACAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTa                                                         <  2:672889/235‑1 (MQ=255)

                                                                                                                                                                                          tCATATACGCGAT‑GTTGT‑CGTCCTTCCACGGTGC‑CGGAGCGGTTTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTAc                                                        <  1:1102879/250‑1 (MQ=255)

                                                                                                                                                                                               taCGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTCCGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTTCTCTCGGCTTCGGTTACTTCaa                                                   >  2:757999/1‑250 (MQ=255)

                                                                                                                                                                                                  gcgAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAtgt                                               >  1:972079/1‑251 (MQ=255)

                                                                                                                                                                                                   cgAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAtgtg                                              >  2:258790/1‑251 (MQ=255)

                                                                                                                                                                                                    gAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                             >  2:277289/1‑251 (MQ=255)

                                                                                                                                                                                                     aT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgcg                                            >  2:721780/1‑251 (MQ=255)

                                                                                                                                                                                                          tgt‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtc                                        >  1:42704/1‑251 (MQ=255)

                                                                                                                                                                                                            ggccgtacgcccaggggccgggAGCGGTGTT‑GTTTTTCCCC‑GCCCCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtc                                        <  2:755208/231‑1 (MQ=255)

                                                                                                                                                                                                              gtcttagtgctatgggggtggggggTGTTCGTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGTCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCGTTGCTCCCGTCGTCGGTTCCTTCAAATGTGCGCTtc                                        <  2:245785/227‑1 (MQ=255)

                                                                                                                                                                                                               gTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCAt                                    >  2:524238/1‑251 (MQ=255)

                                                                                                                                                                                                                tACGTCCACGGTGC‑CGGGGCGGTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATc                                   >  1:739764/1‑251 (MQ=255)

                                                                                                                                                                                                                      cccagggtgcgggggggttttgttttttCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCaa                               <  2:788059/228‑1 (MQ=255)

                                                                                                                                                                                                                                  ggggttgtt‑ttTTTTCCCC‑GCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTGCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACtt                  <  2:203576/246‑1 (MQ=255)

                                                                                                                                                                                                                                     ggTGTT‑GTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTgg               >  1:622714/1‑251 (MQ=255)

                                                                                                                                                                                                                                      gtgtT‑TTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGa              <  1:983534/251‑1 (MQ=255)

                                                                                                                                                                                                                                        gtt‑gttTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACCCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTCGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGATTACTTCAAATGTGCGCGTCTCATCCCTCTGAAGCTGTACTTTGGAtt            >  1:513934/1‑251 (MQ=255)

                                                                                                                                                                                                                                          t‑gttTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCt          >  2:576377/1‑251 (MQ=255)

                                                                                                                                                                                                                                               tttCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGCCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCGGTACTTTGGATTCTAtc       <  1:248485/250‑1 (MQ=255)

                                                                                                                                                                                                                                                ttCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtct      <  1:161308/250‑1 (MQ=255)

                                                                                                                                                                                                                                                 ccACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc     <  1:392896/249‑1 (MQ=255)

                                                                                                                                                                                                                                                   ccc‑gccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTc  <  2:972079/250‑1 (MQ=255)

TGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAG‑CCCAGCATTT‑TGAGCTGGTTTTTCATATACGCGAT‑GTTGT‑CGTACGTCCACGGTGC‑CGGAGCGGTGTT‑GTTTTTCACC‑GCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTC  >  NC_000913/674275‑674762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`