Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	178.2 / NA	48	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/20);  total (28/20)

ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485959‑3486446                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   aCAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCtt                                                                                                                                                                                                                                                >  1:245047/1‑251 (MQ=255) aCAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCt                                                                                                                                                                                                                                                 <  1:290790/250‑1 (MQ=255)   aGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTAt                                                                                                                                                                                                                                              >  1:38616/1‑251 (MQ=255)     aaaTACATGGATGTATTGCATGTATGCAAAGGACGTCACATTTCCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCCCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTc                                                                                                                                                                                                                                            <  2:253366/250‑1 (MQ=255)             tGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGt                                                                                                                                                                                                                                    >  1:192187/1‑251 (MQ=255)                            aTGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAAc                                                                                                                                                                                                                     <  1:59299/250‑1 (MQ=255)                            aTGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACg                                                                                                                                                                                                                    >  2:440543/1‑251 (MQ=255)                                         cacaTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCg                                                                                                                                                                                                       >  2:55325/1‑251 (MQ=255)                                            aTTACCGTGCAGTACAGTTGATAGCACCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACCCTCGAATGGGTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGttc                                                                                                                                                                                                    >  2:217075/1‑250 (MQ=255)                                                     cAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCtg                                                                                                                                                                                           >  2:93024/1‑251 (MQ=255)                                                                   gCCCCTTCCCAGGTGGCGGGAAGCATATTGCGGCAATCCAGAGACAGCGGCGTTATCGGGCTCTGTAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                                                                                                             <  2:143955/251‑1 (MQ=255)                                                                   gCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATc                                                                                                                                                                             >  1:413088/1‑251 (MQ=255)                                                                     cccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGGGCTGATCa                                                                                                                                                                            >  2:328881/1‑250 (MQ=255)                                                                          cccAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACg                                                                                                                                                                      >  2:284177/1‑251 (MQ=255)                                                                              ggTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                                                                                                     >  2:120876/1‑248 (MQ=255)                                                                                tAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAgg                                                                                                                                                                >  1:122303/1‑251 (MQ=255)                                                                                   ggggAAGCATATTTCGGCAAGCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                                                                                                                                             <  2:86093/250‑1 (MQ=255)                                                                                     ggAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTaa                                                                                                                                                            >  1:65460/1‑250 (MQ=255)                                                                                         gCATATTTCGGAAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATACCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGaaa                                                                                                                                                       <  2:268159/251‑1 (MQ=255)                                                                                                tCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAACCAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                                                                                                                                >  2:276293/1‑251 (MQ=255)                                                                                                ccGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCct                                                                                                                                                <  2:270541/250‑1 (MQ=255)                                                                                                               cAGCGGCGTTATCTGGCTCTGGAGAAAGCTTAAAACAGAGGATAACCGCGCATGGTGCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCa                                                                                                                                 <  2:122303/251‑1 (MQ=255)                                                                                                                             ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTg                                                                                                                   <  2:233660/251‑1 (MQ=255)                                                                                                                             ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAAACGCAAACAGACCCGACTATCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGGGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGCCGAAGAGGGTAAAGCAATGATCCTCTCCTATCTGAATCAGGGGGATTTTATTg                                                                                                                   >  2:405533/1‑251 (MQ=255)                                                                                                                              gCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACACGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGGGATTTTATTgg                                                                                                                  >  2:302662/1‑251 (MQ=255)                                                                                                                                          gCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCt                                                                                                      <  1:120876/251‑1 (MQ=255)                                                                                                                                           cTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTg                                                                                                     >  2:250756/1‑251 (MQ=255)                                                                                                                                              ataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGttt                                                                                                  >  1:59950/1‑251 (MQ=255)                                                                                                                                                aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTg                                                                                                 >  1:123374/1‑250 (MQ=255)                                                                                                                                                       gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                         <  2:362445/251‑1 (MQ=255)                                                                                                                                                           aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGCAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGCGGGCCAgg                                                                                     >  2:349059/1‑251 (MQ=255)                                                                                                                                                                       gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTTATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAt                                                                         <  1:331515/251‑1 (MQ=255)                                                                                                                                                                         ttGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATgg                                                                       >  2:287371/1‑251 (MQ=255)                                                                                                                                                                             cAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTAc                                                                   >  2:293934/1‑251 (MQ=255)                                                                                                                                                                                      aaCAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGaaaa                                                          <  1:191611/251‑1 (MQ=255)                                                                                                                                                                                        cAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAAcc                                                        <  2:245047/251‑1 (MQ=255)                                                                                                                                                                                         aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGCGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCg                                                       >  2:8327/1‑251 (MQ=255)                                                                                                                                                                                         aGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCg                                                       >  1:151243/1‑251 (MQ=255)                                                                                                                                                                                             ccGACGCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAGTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAACCCGCCtg                                                   <  2:288242/251‑1 (MQ=255)                                                                                                                                                                                                                 gTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGt                               >  2:127668/1‑251 (MQ=255)                                                                                                                                                                                                                   ctcATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGGAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                             >  1:290643/1‑251 (MQ=255)                                                                                                                                                                                                                    ccatggccaatataaaaagaccccAACCAAGAGCAAGTTTATTC‑CCCGGGGGAAAAAGGCGAAAACGCTGTACAACAGCGTAAAAGGCTGTGTGGCGGGGCTAAACAAAGAGGAAGGGGGAAAAGAAAGGATCCGCTCCTATCGAAATCGGGGTGTTTTTATTGGCAAATTGGGCCTGTTTGAAGGGGGCGAGGAACGTAGCGCGTGGGTGCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACa                            <  2:38616/230‑1 (MQ=255)                                                                                                                                                                                                                     cATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cgg                                                                                                                                                                                                                         <  2:53520/61‑1 (MQ=255)                                                                                                                                                                                                                     cATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑Cgg                                                                                                                                                                                                                         >  1:53520/1‑61 (MQ=255)                                                                                                                                                                                                                       ttGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaa                          >  1:331881/1‑250 (MQ=255)                                                                                                                                                                                                                          ccACATTCATAAGTACCCACCCAAGAGCAAGCTTATTCACCGGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATAAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGACCGGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTCCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAt                      <  1:431515/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaGTACCCAGCCAAGAGCAAGCTTATTCACCAGGGTAAAAAAG‑CGAAAACGCTGTCCTACAGCGTTAAAGGCTCTGGGGCAGGGCTGATAAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATGGGCGAACGGGGCCTGTTGGAAGAGGGCCAGGAACGTAGCGAATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt            <  1:17059/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccAAGAGCAAGCTAATTCACCAGGGTGAAAAAG‑GGAAAACGCTGTACAAAATCTTTAAAGGCTGTGTGGCAGTGCTAATCAAAGACGAAGAGGGTAAAGAAATAATCCCCTCCGATCTGAATGAGGGTGATTTTATTGGCGAAGGGGGCTTGTTTGAAGGGGGCCGGGAACGAAGCGCTTGGGTGCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa  <  1:170962/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAG‑CGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485959‑3486446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑