Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,153	C→T	P58S (CCC→TCC)	lhgD →	L‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,153	0	C	T	100.0%	187.0 / NA	56	P58S (CCC→TCC)	lhgD	L‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/31);  total (25/31)

TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTG  >  NC_000913/2789907‑2790398                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      aggcccaacgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTGCACGTCCgg                                                                                                                                                                                                                                                   >  1:23982‑M2/57‑251 (MQ=255)        acgaaaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGTCAGCCTg                                                                                                                                                                                                                                            >  1:203770‑M2/50‑251 (MQ=255)            aaagctctattgtttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACCACAGCGGCGTGATCCATGCCGGGGGCTATTACACGTCCGGCAGCCTGAAgg                                                                                                                                                                                                                                        >  2:446410‑M2/46‑251 (MQ=255)                        tttacgttgggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGcc                                                                                                                                                                                                                            >  1:60876‑M2/34‑251 (MQ=255)                        tttacgttggcactaaacgcagggagcctccccGCTAAAAGGAATTGAGCGAATGTATGATTTTGTGATTATGGGCGGCGGCATCATCGTCATGTCGTCCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGAAAAAAGAGTCCTCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATGACACGTCCGGCAGCCTGAAGGCGCAGTTTTGc                                                                                                                                                                                                                             <  1:14951‑M2/217‑1 (MQ=255)                            cgttgggcctaaccgcagggagactccccGCGCAAAGGAATGGGGCGGATGTATGATGTTGTGGTTATGGGGGGCGTCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGTCGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGc                                                                                                                                                                                                                        <  2:141603‑M2/222‑1 (MQ=255)                                gggcctaaacgcagggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGcgggg                                                                                                                                                                                                                    >  1:319115‑M2/26‑250 (MQ=255)                                   cctaaacgcagggagaccccccGCCCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTACCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCTGGAAAc                                                                                                                                                                                                                 <  1:269814‑M2/229‑1 (MQ=255)                                             gggagactccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                                                                                                                                                                                                        >  1:6484‑M2/13‑250 (MQ=255)                                                  actccccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGcc                                                                                                                                                                                                  <  1:426000‑M2/244‑1 (MQ=255)                                                       ccGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAACAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCCCAACAGCGGCGTGATCCATGCCGGGGTCGATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTg                                                                                                                                                                                             >  2:367849‑M2/3‑251 (MQ=255)                                                                     ggAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCAGCGGCATGTCGCCCGCCATGCACCTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGCCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCa                                                                                                                                                                               <  1:446410/250‑1 (MQ=255)                                                                       ggCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACTGCAtt                                                                                                                                                                             <  1:448351/250‑1 (MQ=255)                                                                           gATGTATGATTTGGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                                         <  1:348906/251‑1 (MQ=255)                                                                           gATGAATGATTTGGTGATTATGGGCGGCGGCTTCATGGGCATTTCGCCGGCCAGTCAACTGATTGATGCCTATCCGGACGCCGGCATTGCGTG‑GCTGGAAAAAGGGCCCCCCCCGGCCTGGCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGGAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                                         <  2:409373/251‑1 (MQ=255)                                                                                 tGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAAGGCATTCGCTACGAc                                                                                                                                                                    >  1:81035/1‑250 (MQ=255)                                                                                   aTTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAAc                                                                                                                                                                 >  1:83118/1‑251 (MQ=255)                                                                                             tattGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAg                                                                                                                                                        <  1:432074/250‑1 (MQ=255)                                                                                               ttGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATg                                                                                                                                                     >  1:249405/1‑251 (MQ=255)                                                                                                 ggcggcggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGc                                                                                                                                                    <  1:303410/250‑1 (MQ=255)                                                                                                  gcggcggcATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGAGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCAAGCCGGGGTCTATTACACGTCCGGCAGCCTGAAAGCGCAGTTTTGCCTGGCGGGAAACCGCGCCAACAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTg                                                                                                                                                  >  1:414327/1‑251 (MQ=255)                                                                                                          accagcGGCTTGTGGACCGCCATGCATCTGATTGAGGTCTAGCCGGCCGCCCGCATTGCTTG‑GCTGGAAAAACAGGCCGCCCCGCCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGTGGCAAGATGCTGGTTGCCAc                                                                                                                                          <  2:119469/246‑1 (MQ=255)                                                                                                              ccGGCATGACGACCGCCATGCAACTGATTGATGTCTATCCGGCCGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTcc                                                                                                                                      <  2:314249/250‑1 (MQ=255)                                                                                                                ggCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCg                                                                                                                                     >  2:428309/1‑250 (MQ=255)                                                                                                                              cATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCGGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAc                                                                                                                      <  1:295498/251‑1 (MQ=255)                                                                                                                              cATGCAACTGATGAATGTCGATCCGGCCGCCCGCATTGCGTT‑GATGGAAAAAGAGCCCCCCCCGGCCTTTCACCAGAGGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAc                                                                                                                      <  2:300747/251‑1 (MQ=255)                                                                                                                                     cTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcg                                                                                                               >  2:217903/1‑251 (MQ=255)                                                                                                                                      tgagtgatGTCTATCCGGCCGACCGCATTGCGGT‑GCTGGAAAAAGAGTCCGCCCAGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGCAGGCGCAGTTTTGCCTGGCGGGCAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCGACGACAACTGCGGCAAGATGCTGGCTGCCACGTCCGATCTCGAAATGGAACGGATgcgc                                                                                                              >  2:178155/5‑251 (MQ=255)                                                                                                                                         ttgatGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGcc                                                                                                           >  2:35172/1‑251 (MQ=255)                                                                                                                                           gatGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACCGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGAGCGCCtt                                                                                                         >  2:190885/1‑251 (MQ=255)                                                                                                                                             ggTCTATCCGGCCGCCCGC‑TTGGGTTTGCTGGAAAAAGAGCCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTAt                                                                                                       <  2:6484/250‑1 (MQ=255)                                                                                                                                                  aTCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATggg                                                                                                    <  1:131858/249‑1 (MQ=255)                                                                                                                                                          gCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgt                                                                                          >  1:108826/1‑250 (MQ=255)                                                                                                                                                               cATTGCGGT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGa                                                                                     <  1:367849/251‑1 (MQ=255)                                                                                                                                                                    cGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                                <  1:284218/251‑1 (MQ=255)                                                                                                                                                                       tggCTGGAAAAAGAGCCCGCCCCGGCCTTTCCCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTGCACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGt                                                                                <  2:23982/247‑1 (MQ=255)                                                                                                                                                                             aaaaaaGAGTCCCCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAgcg                                                                        <  2:181562/250‑1 (MQ=255)                                                                                                                                                                                    agTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACCGTGGCGAACGGTATCGAGCGCGAATgg                                                                 >  2:285318/1‑251 (MQ=255)                                                                                                                                                                                       ccGCCCCGGCCTGTCACCAGGCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTAGTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCtt                                                              <  2:204799/251‑1 (MQ=255)                                                                                                                                                                                             cGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGcc                                                        <  1:157437/251‑1 (MQ=255)                                                                                                                                                                                              ggCCTGTCCCAAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcg                                                       <  2:81035/251‑1 (MQ=255)                                                                                                                                                                                                  tGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGACACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgacg                                                    >  2:278404/1‑250 (MQ=255)                                                                                                                                                                                                     ccccAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                                                <  2:376941/249‑1 (MQ=255)                                                                                                                                                                                                      aCCAGACGGGCCACAACAGCAGAGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGCATCGAGCGCGAATGGCTTAACGCCGACGAACTg                                               >  2:397665/1‑251 (MQ=255)                                                                                                                                                                                                                  acaacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagcgcga                                    >  1:91879/1‑250 (MQ=255)                                                                                                                                                                                                                    aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcg                                             >  2:156103/1‑239 (MQ=255)                                                                                                                                                                                                                    aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcg                                             <  1:156103/239‑1 (MQ=255)                                                                                                                                                                                                                             gTGATCCATGCCGGGGTCTATTACCCGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGACCGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAc                        <  1:1845/251‑1 (MQ=255)                                                                                                                                                                                                                             gTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGACCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAc                        <  2:340121/251‑1 (MQ=255)                                                                                                                                                                                                                               gATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCg                      >  1:157067/1‑251 (MQ=255)                                                                                                                                                                                                                                      gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgag                                         <  2:161654/225‑1 (MQ=255)                                                                                                                                                                                                                                      gCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgag                                         >  1:161654/1‑225 (MQ=255)                                                                                                                                                                                                                                      gCCGGGGTCTATTACACGTCCGGAAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTCGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcgg               <  2:50949/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccGGGGTCTATTACACGTCCGGCGGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACTGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTcggc              <  2:414327/251‑1 (MQ=255)                                                                                                                                                                                                                                          gggTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCggcggc           >  1:377382/1‑251 (MQ=255)                                                                                                                                                                                                                                                   acacGTCCGGCAGCCTGAAGGCGCAGTTTTGCCGGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGTGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTtgtg  <  2:176355/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGCGCCAGCGTGGCTATTTCGCTTACGCCTCTAACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTCTATCCGGACGCCCGCATTGCGTT‑GCTGGAAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTG  >  NC_000913/2789907‑2790398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑