Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,481,795 G→A Q128* (CAG→TAG)  emrY ← tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,481,7950GA95.2% 73.4 / ‑6.4 21Q128* (CAG→TAG) emrYtripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/2);  minor base C (0/1);  total (18/3)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTGTGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTT  >  NC_000913/2481570‑2482024
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       
ccGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGcc                                                                                                                                                                                                               >  2:68985/1‑251 (MQ=255)
       tgTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAAcc                                                                                                                                                                                                        >  2:87049/2‑251 (MQ=255)
                                 agGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGc                                                                                                                                                                              >  1:52991/1‑251 (MQ=255)
                                                                cATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACAtt                                                                                                                                                >  1:85503/1‑250 (MQ=255)
                                                                     ggACATTGATTAAAAATATCCAACCCCGGCTAAAGTTATCACAAATATAACCGCCCACTATCGGCCCACATATCGGAGCGATAATCACGGCCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGa                                                                                                                                           >  1:344588/1‑250 (MQ=255)
                                                                             aTTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCg                                                                                                                                   >  2:51730/1‑250 (MQ=255)
                                                                             aTTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCg                                                                                                                                   >  2:40960/1‑250 (MQ=255)
                                                                                aaaaaTATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGaaa                                                                                                                                >  1:24665/1‑250 (MQ=255)
                                                                                         cAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGa                                                                                                                                  >  1:202556/1‑239 (MQ=255)
                                                                                                   tAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAACAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTaa                                                                                                            >  2:124669/1‑250 (MQ=255)
                                                                                                                                     cccACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGCGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAAcc                                                                         >  1:290705/1‑251 (MQ=255)
                                                                                                                                               ggAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAAc                                                               >  2:168298/1‑251 (MQ=255)
                                                                                                                                                             ggTCATTGACAATAAGGCCAAACAAAATTTTCTTTTGTCGGGCGAA‑TAATTCTAAAATAAAAAACCTTATAACAGTGAAATT‑ACCGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATgg                                                 <  1:124669/251‑1 (MQ=255)
                                                                                                                                                                            ggcaagaaaaaatttttttttttCTG‑CGGAATAATTCCTAAAAAAAAAACTCTATGAAAG‑GGAATTTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCa                                   <  2:85503/234‑1 (MQ=255)
                                                                                                                                                                              ccAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCACCCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAAc                                >  2:98270/1‑251 (MQ=255)
                                                                                                                                                                              ccAGAGCAAATGTTCTTGTTTCTGGAGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAAc                                >  1:86442/1‑251 (MQ=255)
                                                                                                                                                                                    cAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAACCGAGGTg                          >  2:16798/1‑251 (MQ=255)
                                                                                                                                                                                                       cGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGt       >  2:21451/1‑251 (MQ=255)
                                                                                                                                                                                                       cGGA‑TAATTCCTTAATAATAAACTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGAAAAAATAATCTTAATTCGCCTATTCTTTGTGCAAACCTGCCAGAAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGt       >  1:47601/1‑251 (MQ=255)
                                                                                                                                                                                                             atttcataaaaaaaaaaCTCTCTGACATTGAAATT‑ACCCCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTAGTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGtt  <  2:294484/237‑1 (MQ=255)
                                                                                                                                                                                                                           aaaCTCTATGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGaa                                                                                                                                          >  1:376337/1‑102 (MQ=255)
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CCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGA‑TAATTCCTTAATAATAAACTCTGTGACAGTGGAATT‑AACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAA‑GTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTT  >  NC_000913/2481570‑2482024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑