Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,954	A→G	intergenic (+23/+78)	yoeF → / ← tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,954	0	A	G	100.0%	167.2 / NA	43	intergenic (+23/+78)	yoeF/tmaR	putative uncharacterized protein YoeF/PTS system regulator TmaR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/23);  total (20/23)

GCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTC  >  NC_000913/2078707‑2079194                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 agactttctcgtgttaacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGTTGAGGatg                                                                                                                                                                                                                                               >  2:352854‑M2/224‑251 (MQ=255)                aacgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATacac                                                                                                                                                                                                                                <  1:366924‑M2/43‑1 (MQ=255)                  cgtccgacaaaatgcgtcattgacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATacac                                                                                                                                                                                                                                >  1:133700‑M2/207‑249 (MQ=255)                                     ttgacagcggttactgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctcttgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaat                                                                                                                                                                                                           >  2:255288‑M2/188‑251 (MQ=255)                                       gacagcggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaatg                                                                                                                                                                                                          <  1:145438‑M2/65‑1 (MQ=255)                                             ggtttctgtggattgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTg                                                                                                                                                                                                   >  1:332980‑M2/180‑251 (MQ=255)                                                          tgacacatttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTc                                                                                                                                                                                      >  1:180814‑M2/167‑251 (MQ=255)                                                               catttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTGCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGa                                                                                                                                                                                 <  1:382500‑M2/90‑1 (MQ=255)                                                                  ttcattcagataatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgacgatAATTTCGATTGGTTCACGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAGTGGGGGTTTCCGTGCAACCCTCATTGAAt                                                                                                                                                                               <  2:358314‑M2/92‑1 (MQ=255)                                                                  ttcaatcagataatgaattaatgctactgccggaacaaccagcaaacgggcatggtgttctgaaatccaggatattgattcaacgtcagcgacagatctgcgcggtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACATATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAAt                                                                                                                                                                               <  2:209722‑M2/92‑1 (MQ=255)                                                                      ttcagataatgaattcatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtcaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGAAGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATaaaa                                                                                                                                                                           >  1:245689‑M2/155‑250 (MQ=255)                                                                       tcagataatgaattaatgctactgccggaacacccagcaaacgggcattgtgttctgaaatccaggctattgagtcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattacccggaatggtggcgactatAATTTCGAGTGGTTCGCGGATGAGGATGATGACGGTTATACACAGATAAGAATCAAGGAGCAATGAGGGTTGCCGGTCTACCCGCGGTGAATAAAAc                                                                                                                                                                          <  2:164107‑M2/97‑1 (MQ=255)                                                                             aatgaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAAtt                                                                                                                                                                   <  2:133700‑M2/104‑1 (MQ=255)                                                                                gaattaatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTAc                                                                                                                                                                 <  1:250187‑M2/106‑1 (MQ=255)                                                                                     aatgctactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgc                                                                                                                                                            <  1:37863‑M2/111‑1 (MQ=255)                                                                                          tactgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGcc                                                                                                                                                      >  1:65978‑M2/135‑251 (MQ=255)                                                                                           actgccggaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCg                                                                                                                                                     >  1:190245‑M2/134‑251 (MQ=255)                                                                                                  gaacaaccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTc                                                                                                                                               >  2:212399‑M2/127‑250 (MQ=255)                                                                                                        ccagcaaacgggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGc                                                                                                                                        >  1:23040‑M2/121‑251 (MQ=255)                                                                                                                  ggcattgtgttctgaaatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTGCGCCGTTTTTCATTTCGCCCATAGCttt                                                                                                                              <  2:394605‑M2/141‑1 (MQ=255)                                                                                                                                 aatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTgg                                                                                                                   >  1:17796‑M2/96‑247 (MQ=255)                                                                                                                                 aatccaggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGat                                                                                                               <  1:172558‑M2/156‑1 (MQ=255)                                                                                                                                     caggctattgattcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATcg                                                                                                           <  1:108429‑M2/160‑1 (MQ=255)                                                                                                                                                 tcaacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCttt                                                                                                <  1:7783‑M2/171‑1 (MQ=255)                                                                                                                                                  caacgtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTg                                                                                               <  1:281241‑M2/172‑1 (MQ=255)                                                                                                                                                      gtcagcgacagatctgcgctgtaaaagaacttgtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGagag                                                                                          <  1:427226‑M2/177‑1 (MQ=255)                                                                                                                                                                          gcaaaagaacttgtaaaccaacgtaatagaattatccggactggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCTCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGgcatttttgttgctgg                                                                      >  2:19895‑M2/55‑244 (MQ=255)                                                                                                                                                                                      gtaaaacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCTTCAAcg                                                           <  1:273565‑M2/208‑1 (MQ=255)                                                                                                                                                                                         aaacaacgtaatagaattatgcggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATAGCGCGGCGTTCTTTGGAGAGCTCGGCATTTGTGATGATGTAATCGTCAACGCGAt                                                       <  2:330734‑M2/212‑1 (MQ=255)                                                                                                                                                                                          aacaacgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATc                                                      <  1:212399‑M2/213‑1 (MQ=255)                                                                                                                                                                                               cgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgcttc                                                                                                                                                         <  1:428031‑M2/114‑1 (MQ=255)                                                                                                                                                                                               cgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTActtcgcttc                                                                                                                                                         >  2:428031‑M2/34‑147 (MQ=255)                                                                                                                                                                                               cgtaatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCAt                                                 >  1:133343‑M2/34‑251 (MQ=255)                                                                                                                                                                                                  aatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGt                                              >  2:395807‑M2/31‑251 (MQ=255)                                                                                                                                                                                                  aatagaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGt                                              <  1:324810‑M2/221‑1 (MQ=255)                                                                                                                                                                                                     agaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCAc                                           >  1:452242‑M2/28‑251 (MQ=255)                                                                                                                                                                                                      gaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGCTCTTCATAGTCAcc                                          >  1:403006‑M2/27‑251 (MQ=255)                                                                                                                                                                                                      gaattatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCAcc                                          <  2:332980‑M2/225‑1 (MQ=255)                                                                                                                                                                                                        attatccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCtt                                        >  1:2606‑M2/25‑251 (MQ=255)                                                                                                                                                                                                         ttgtccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGGGATACACAGATAAGAATCAATGAGCAATGAGGGAGGCCGGGCAGCACTCATTGAATAAAACGGGAATAACTTAGCTTCGCCGTTTTTCATTTCGCGCATAGCTTTCAGCTTGTTGGAGATATCGCGGCGGTGTTGGGAGAGCTCGGCATTTGTGATGATGTAATCGTCAACGCGATCGTCATAGTCAcctgt                                       >  2:195764‑M2/24‑249 (MQ=255)                                                                                                                                                                                                           atccggaatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAACCGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTc                                      <  2:85591‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                 aatggtggcgactatAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTgg                               <  1:417928‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                               tcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTc  >  2:187859/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GCGCTGAAGCAGGAATTAGAGGTAAAAACGCCCTACCGGGCCATGAATCACCCGGTCATTGGAGTAGTAACCAAAGCAGATTTAGCCAGCATGGAACAAATATCTTTGGTGAAAAGTTGGTTACGGGAAGCTGGAGCGCACAACGTGTTAGTAACCAGTGCAGTTAATAACAATGGAGTTACAGAACTTTTTGCCTTGCTGCATACAGAAGAAGGCTGTTGTTAATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAAAACGGGAATTACTTCGCTTCGCCGTTTTTCATTTCGCCCATAGCTTTCAGCTTTTTGGAGATATCGCGGCGTTCTTTGGAGAGCTCGGCATTTTTGATGATGTAATCGTCAACGCGATCTTCATAGTCACCTTTCATGCTGGCGATGATGCCCTGGATTGCTTCAACGCTC  >  NC_000913/2078707‑2079194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑