Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,992	C→A	intergenic (‑209/‑26)	leuS ← / → ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,992	0	C	A	100.0%	220.2 / NA	55	intergenic (‑209/‑26)	leuS/ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/31);  total (24/31)

TTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAGAA  >  NC_000913/674745‑675236                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     tttGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGt                                                                                                                                                                                                                                                   >  1:263579/1‑251 (MQ=255)           aTCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGAccc                                                                                                                                                                                                                                          <  2:93159/250‑1 (MQ=255)                  ccGGGCGGTATTGCTCTTGCAGGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGAt                                                                                                                                                                                                                                   <  1:60701/250‑1 (MQ=255)                        ggTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGg                                                                                                                                                                                                                            >  1:214289‑M1/1‑250 (MQ=255)                          tATTGCTCTTGCTTGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgc                                                                                                                                                                                                                           >  1:267688‑M1/1‑248 (MQ=255)                           aTTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcat                                                                                                                                                                                                                         >  2:176335‑M1/1‑247 (MQ=255)                              gCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatcc                                                                                                                                                                                                                       <  2:27577‑M1/250‑7 (MQ=255)                                tctTGAATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggg                                                                                                                                                                                                                    >  1:455759‑M1/1‑242 (MQ=255)                                   tGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtga                                                                                                                                                                                                                  <  1:103280‑M1/250‑12 (MQ=255)                                   tGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggt                                                                                                                                                                                                                    <  2:115973‑M1/248‑10 (MQ=255)                                        ggCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgc                                                                                                                                                                                                            <  1:199976‑M1/251‑18 (MQ=255)                                                gTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccgttgagcgtgccgaaatat                                                                                                                                                                                                    <  1:94164‑M1/251‑26 (MQ=255)                                                gTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatat                                                                                                                                                                                                    <  2:314954‑M1/251‑26 (MQ=255)                                                   gTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatggg                                                                                                                                                                                                 <  1:352114‑M1/251‑29 (MQ=255)                                                     ccTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggat                                                                                                                                                                                               >  1:105618‑M1/1‑221 (MQ=255)                                                                 aCGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggca                                                                                                                                                                                    <  1:116253‑M1/250‑42 (MQ=255)                                                                    gCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgat                                                                                                                                                                                >  1:360895‑M1/1‑206 (MQ=255)                                                                       aCAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgata                                                                                                                                                                               >  1:265267‑M1/1‑203 (MQ=255)                                                                       aCAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgata                                                                                                                                                                               >  2:130552‑M1/1‑203 (MQ=255)                                                                       aCAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgata                                                                                                                                                                               >  2:379609‑M1/1‑203 (MQ=255)                                                                          aaTGTAGCGTTGAGGTGGTTTTTCAGATCAGCATAGCATAGCCCAAACGTCAGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTATATTCTTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataaga                                                                                                                                                                            >  1:122049‑M1/1‑200 (MQ=255)                                                                           aTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagg                                                                                                                                                                         <  1:263705‑M1/251‑53 (MQ=255)                                                                               aGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggatt                                                                                                                                                                     >  2:130380‑M1/1‑195 (MQ=255)                                                                                    tGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattattt                                                                                                                                                                 <  1:130380‑M1/250‑61 (MQ=255)                                                                                     gAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttac                                                                                                                                                               >  1:71943‑M1/1‑189 (MQ=255)                                                                                       ggtggtTTTTCAGATCCGCAGAGCATAGCCCAAACGTCCGCGTCAAAACAGCATTTCGCGCACTCGTCGTTGGAATGATGGCCGGATTACTCATACAATAATTTCCAGAGTTTGTGGGCGTAATAGCAAAGCAATGAACAAAGAACGTCTATTATTAGAGTAAGTTAACGGCCCGGGAGATGAAACGgcatccggtgagcgggccgaaatatgggatgtattccggcacgataagaatggattatttacgt                                                                                                                                                             <  2:384279‑M1/251‑65 (MQ=255)                                                                                             ttttCAGATCCGCATCGCATAGCCCAAACGACCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCTGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcctccggtgagcgtgccgaaatatggtatgtatgccggcacgataagaagggattatttacgtcgctg                                                                                                                                                        <  1:127716‑M1/250‑70 (MQ=255)                                                                                                         catagcatagCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatc                                                                                                                                           <  1:455095‑M1/251‑83 (MQ=255)                                                                                                         catagcatagCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGATGAAATGATGCCCGGATTACTCATACATTAATTTACAGCGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatc                                                                                                                                           >  1:402636‑M1/1‑169 (MQ=255)                                                                                                           tagcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatca                                                                                                                                          >  2:97739‑M1/1‑167 (MQ=255)                                                                                                                    ccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagc                                                                                                                                <  1:90386‑M1/251‑94 (MQ=255)                                                                                                                       aaCGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaa                                                                                                                             <  1:437174‑M1/251‑97 (MQ=255)                                                                                                                            ccGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtc                                                                                                                                                            >  1:134425‑M1/1‑150 (MQ=255)                                                                                                                            ccGCGGCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtc                                                                                                                                                            <  2:134425‑M1/215‑66 (MQ=255)                                                                                                                             cgcgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaa                                                                                                                       <  2:11362‑M1/251‑103 (MQ=255)                                                                                                                               cgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaaca                                                                                                                     <  1:200465‑M1/251‑105 (MQ=255)                                                                                                                                              tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcaccc                                                                                                      >  2:361594‑M1/1‑132 (MQ=255)                                                                                                                                              tCGCGCACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacc                                                                                                       <  1:169969‑M1/250‑119 (MQ=255)                                                                                                                                                gcgcACTCGACGTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATGATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccac                                                                                                    <  1:176335‑M1/251‑122 (MQ=255)                                                                                                                                                           gTTGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagtt                                                                                         <  2:55443‑M1/251‑133 (MQ=255)                                                                                                                                                             tGAAATGATGCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaagccaaacaatgccgtcagcacccacagcggccagttg                                                                                        <  1:262439‑M1/250‑134 (MQ=255)                                                                                                                                                                 atgatgCCCGGATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacacacagcggccagttgccggt                                                                                   >  1:48587‑M1/1‑113 (MQ=255)                                                                                                                                                                           gATTACTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcccccacagcggccagttgccggtacgtgcgtat                                                                         <  2:241399‑M1/251‑149 (MQ=255)                                                                                                                                                                                                  gagTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtaggtcccggtggtggcgg                                                  >  2:357827‑M1/1‑80 (MQ=255)                                                                                                                                                                                                     tttGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtctggcggtcggcgtcac                                               >  2:253459‑M1/1‑77 (MQ=255)                                                                                                                                                                                                             cGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgaatcccgtgggccgcggcatcataaa                                         >  2:406201‑M1/1‑69 (MQ=255)                                                                                                                                                                                                                    gcaaagcaaGTAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcaaccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacac                                <  2:455759‑M1/251‑190 (MQ=255)                                                                                                                                                                                                                         gcaaGGAACAAAGAACGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtgggcggcgtcacgttagtggttaacacctcgc                           >  2:306673‑M1/1‑57 (MQ=255)                                                                                                                                                                                                                                      aaCGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttaatggttaacacctcgcgggtgaactgcgg              >  2:82816‑M1/1‑44 (MQ=255)                                                                                                                                                                                                                                       aCGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacc                               >  1:18266‑M1/1‑43 (MQ=255)                                                                                                                                                                                                                                       aCGTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacc                               <  2:18266‑M1/233‑191 (MQ=255)                                                                                                                                                                                                                                         gTCTATTATTATAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggtt           <  1:218776‑M1/251‑211 (MQ=255)                                                                                                                                                                                                                                             attattatAGTAAGTTAGCGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaaggccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggatc          <  2:411874‑M1/248‑212 (MQ=255)                                                                                                                                                                                                                                             attattatAGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgtgatca         <  1:145207‑M1/249‑213 (MQ=255)                                                                                                                                                                                                                                                   ataGTAAGTTAACGACCCGGGAGATGAAACGgcatccggtgagcgtgccgaaatatgggatgtattccggcacgataagaagggattatttacgtcgctgacgcagactcatcaacacagcagcaaaaccaaacaatgccgtcagcacccacagcggccagttgccggtacgtgcgtatggtgtgagtccggtggtcggcgtcacgttagtggttaacacctcgcgggtgaactgcgggatcatcgcctg  <  2:265267‑M1/250‑220 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     TTTGGATTCTATCTCTTCCGGGCGGTATTGCTCTTGCATGGCAGCCAGTGGTCCTGTTTTCAATACGGCTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCGAACGTGATATCGACGCACTGGTGGAACAGGCGCGCGAGCGCGTAATAAAAACAGGGGAGTTAACGCGAACCGAGGTCGATGAGCTGACGCGAGCTGTCAGACGTGACCTGGAAGAGTTCGCCATGAGCTATGAAGAGAGCCTGAAAGAAGAA  >  NC_000913/674745‑675236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑