Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	174.1 / NA	48	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

TTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTC  >  NC_000913/674274‑674759                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ttGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCtt                                                                                                                                                                                                                                             >  1:384279/1‑251 (MQ=255)      ggttcgCCAGTGCGGTCTGGTCGTTCGGGCCCCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTTAAGAATTTCTGTTCCCACCGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCgca                                                                                                                                                                                                                                        <  1:314629/250‑1 (MQ=255)          cgCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCgcaggc                                                                                                                                                                                                                                     >  1:100885/1‑250 (MQ=255)                                               gCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTCCGTCCACGGTGCCGGAGCGGTGTTTTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGtt                                                                                                                                                                                               <  1:403889/251‑1 (MQ=255)                                                   aaGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGTCGATTTTg                                                                                                                                                                                           >  2:455095/1‑251 (MQ=255)                                                       cttcttATATACAAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGCGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                        >  2:169969/1‑250 (MQ=255)                                                             atataCCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAc                                                                                                                                                                                 >  2:287402/1‑251 (MQ=255)                                                                 aCCAGGCTTTTTTTATACAGCGCGTTGAAGAATTTCTGTTCCAAACGGTAGTATTCCGGCGTACAGGTTGCCACCTCGGGGCTCCAGTCATAACCAAAGCCCAGCATTTGGAGCTGGTTTTTCATATACGCGATGTTGTCGTACTTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTg                                                                                                                                                                             <  1:208689/251‑1 (MQ=255)                                                                            tttATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATAAGCTGGTAGCGGGCg                                                                                                                                                                   >  2:79844/1‑250 (MQ=255)                                                                              tataCAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                >  1:238665/1‑251 (MQ=255)                                                                                 ccAGCTCGGTGAAGAATTTTTGTTCCCAGCGGTGGTATTCCGGCGTCCAGGTTGCTAGTTCGCGGTTCCAGTAATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATTTTGTCGTACGCCCACGGTGCCGGAGGGGTGTTGTTTTTCACC‑GCTACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCAc                                                                                                                                                              <  1:121268/249‑1 (MQ=255)                                                                                  cAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCAGCAGGACGTTTTTGCCCAGCATGCGCTGGTCGCGGGCGATCACGt                                                                                                                                                            >  1:438736/1‑251 (MQ=255)                                                                                       cGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCg                                                                                                                                                       >  1:270889/1‑251 (MQ=255)                                                                                           gaagaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGAGCACGTCACCGATgg                                                                                                                                                   >  2:90386/1‑251 (MQ=255)                                                                                            aagcaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGCGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCAAGCATACGCTGGTAGCGGCTGATCACGTCACCGATGgt                                                                                                                                                  >  2:263705/5‑251 (MQ=255)                                                                                                   tCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCGCGTCACCGATGGTGTAGtt                                                                                                                                            >  1:323076/1‑250 (MQ=255)                                                                                                           cAACGGTAGTATTCCGGAGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCCTACGCTGGTAGCGGGCGATCACGTAACCGATGGTGTAGTTACGTACGTg                                                                                                                                   >  1:91169/1‑251 (MQ=255)                                                                                                               ggTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATACCCAAAGCCCGGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTCGTCCGTCCCCGGTGCCGGGGCGGTTTTGTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGccc                                                                                                                               <  1:70562/251‑1 (MQ=255)                                                                                                                  agtaTTCCGGCTTAAAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCAGGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCAtg                                                                                                                            <  1:263781/251‑1 (MQ=255)                                                                                                                        ccGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGTTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGt                                                                                                                      <  1:148789/251‑1 (MQ=255)                                                                                                                         cGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGGGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTc                                                                                                                     <  1:97839/251‑1 (MQ=255)                                                                                                                                cAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGAAGGTGTAGTTACGTCCGTGGCCCATGTGTAGTCgccc                                                                                                                 >  2:307247/1‑245 (MQ=255)                                                                                                                                         aGCTCGCGGCTCCATTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTCGTACGTCCACGTGGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAggg                                                                                                     <  1:122029/251‑1 (MQ=255)                                                                                                                                             cgcgGCTCCAGTCATAACCAAACCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGc                                                                                                 <  1:13114/251‑1 (MQ=255)                                                                                                                                               cgGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                               >  1:115973/1‑251 (MQ=255)                                                                                                                                               cgGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGAAGCGGGCGATCACGTCCCCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                               >  2:116253/1‑251 (MQ=255)                                                                                                                                                    ccgttaaaaacaaaaGCCCAGCTTTGTGAGCTGGTTTTTCATATACGCGATGTTGTCGTTCGTCCACGGGGCCGGAGCGGTGTTTTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGa                                                                                            <  2:26438/237‑1 (MQ=255)                                                                                                                                                                aaaGCCCAGCTTTTTGAGTTGGTTTTTCATATCCGCGATGTTGTCGTCCGCCCGCGGGGCCGGAGCGGTGTTGTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACt                                                                              <  2:170979/251‑1 (MQ=255)                                                                                                                                                                aaaGCCCAGCATTTTGTGTGGGTTTTTCATATTCGCGATGTTGTCGCCCGCCCACGGTGCGGGGGCGGTGTGTTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACt                                                                              <  2:265775/251‑1 (MQ=255)                                                                                                                                                                       aGCATTTTGAGCTGGTTTTTCATATACGCGGTGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCttt                                                                       >  1:180548/1‑251 (MQ=255)                                                                                                                                                                              tgggCTGTTTTTACATATCCGCGATGTTGTCGTACGCCCCCGGTGCGGGAGGGTTTTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCtctc                                                                 <  2:376836/247‑1 (MQ=255)                                                                                                                                                                               gAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGt                                                               >  1:93159/1‑251 (MQ=255)                                                                                                                                                                                    ggTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCtt                                                            <  1:26027/249‑1 (MQ=255)                                                                                                                                                                                          ccatattcgctttgttggCGTACGTCGACGGTGCGGGCGGGGTGTTTTTTCTCCCC‑GTCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTAc                                                     <  2:15439/238‑1 (MQ=255)                                                                                                                                                                                           cATATCCGCGATGTTGTCGTACGTCCACGGTGCCGGGGCGGTGTTTTTTTCCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACt                                                    <  1:134328/250‑1 (MQ=255)                                                                                                                                                                                           cATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCggtgcacagaatat                                                   >  1:215286/1‑238 (MQ=255)                                                                                                                                                                                                  gcgAAGTTGTCGCCCGTCCACGGAGCCGGAGCGGTGTTGTTTTTCACC‑GACACGCCTTCCGCAGGCAGACCAAACGCGTCCAAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGATGGTAGCGGGCGATCACGTCCCTGCTGGTGTAGTTACGTACGTAGCCCATGTGCAGTCGACCAGAAGGATAGGGAAGAATAGACAGGCAGTAATACTTATCTATGCTTTCGTCTTCGGTTACTTctactgt                                            >  2:145207/1‑245 (MQ=255)                                                                                                                                                                                                    gATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTgc                                          >  1:22431/1‑251 (MQ=255)                                                                                                                                                                                                       gttgtCGTCCGTCCACGGTGCCGGAGCGGTTTTGTTTTTCCCC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCt                                       <  1:116689/251‑1 (MQ=255)                                                                                                                                                                                                       gttgtCGTACGTCCACGGTGCCGGAGGGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCt                                       <  1:181910/251‑1 (MQ=255)                                                                                                                                                                                                         tgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCtt                                      <  1:119240/250‑1 (MQ=255)                                                                                                                                                                                                            cGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGAAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtctc                                   >  1:241399/1‑250 (MQ=255)                                                                                                                                                                                                                 gTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCa                             <  1:429085/251‑1 (MQ=255)                                                                                                                                                                                                                  tCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCa                             >  1:314954/1‑250 (MQ=255)                                                                                                                                                                                                                   ccagggggcgggtgCGGTGT‑GTTTTTCACCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAAt                           <  2:270889/238‑1 (MQ=255)                                                                                                                                                                                                                                     ttgttgtttaccc‑gccaCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTGCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt          <  2:272698/239‑1 (MQ=255)                                                                                                                                                                                                                                     gttgttTGTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGAt          <  2:238665/250‑1 (MQ=255)                                                                                                                                                                                                                                      ttgttTTTCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc        >  1:267880/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttCACC‑GCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc  >  1:379160/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccccc‑gccccgccTTCCGCAGGCCGACCAAACGCGACCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTAtctc  <  2:431574/247‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACC‑GCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTC  >  NC_000913/674274‑674759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑