Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,498	C→G	R165P (CGC→CCC)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,498	0	C	G	100.0%	166.0 / NA	45	R165P (CGC→CCC)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/19);  total (26/19)

CGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTC  >  NC_000913/4312260‑4312742                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     cGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTtgt                                                                                                                                                                                                                                          >  1:330337/1‑251 (MQ=255)  gTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGCAGCGGACATCCACATGCTGTGAGCATCAGGATCGGCCTGACAGCGTACGCCAATGGGCAAGAACTtgtg                                                                                                                                                                                                                                         >  2:221865/1‑251 (MQ=255)    ttCCGGGGCTGGTGAGCAAATAATATGATAGGCCAGTTTCGCTATCGGTGACTGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCCCTACCAGCACCACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCt                                                                                                                                                                                                                                       >  2:351388/1‑251 (MQ=255)      ccGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGt                                                                                                                                                                                                                                     <  1:342021/251‑1 (MQ=255)        ggggCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTAc                                                                                                                                                                                                                                   <  1:15422/251‑1 (MQ=255)                       ataatatgatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGc                                                                                                                                                                                                                    <  2:423084/251‑1 (MQ=255)                          atatgatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAtt                                                                                                                                                                                                                 <  1:252041/251‑1 (MQ=255)                                 cGGCCAGTTTCGCTATCGGTGAATGGAAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGAtcct                                                                                                                                                                                                          >  1:388380/1‑251 (MQ=255)                                 cGGCCAGTTTCGCTAGCGGTGAATGGTAGCTATGGGTTATACAAATAATCTGTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGAtcct                                                                                                                                                                                                          <  2:128774/251‑1 (MQ=255)                                      aGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCTCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGc                                                                                                                                                                                                     <  1:195506/251‑1 (MQ=255)                                                   gTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATcc                                                                                                                                                                                        >  1:40522/1‑251 (MQ=255)                                                        tGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTg                                                                                                                                                                                   >  1:111345/1‑251 (MQ=255)                                                           tAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTTTACGGCCGCTTTTACATCACTGGTTCCCCCGGAATAGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGCGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATCGCATTTGATCCTCCGGCACCGTACAAATccagaggtct                                                                                                                                                                                >  2:345266/1‑243 (MQ=255)                                                                         aCAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGaaa                                                                                                                                                                   >  2:38147/1‑250 (MQ=255)                                                                           aaaTAATCTTTGCCTCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGGTCGCCCGGAATGGGTCACTACCAGCACAGCATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATATCATATGAACCTCCGGCACCGTACAAATCCCGCTTTCTGGCCTGATAGAAAAAg                                                                                                                                                                <  2:145685/251‑1 (MQ=255)                                                                                    tggCCCCGTTCGTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTGCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGGCTGGCCTGATAGAAAAAGCGggcggc                                                                                                                                                        <  2:176314/248‑1 (MQ=255)                                                                                      gCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCAc                                                                                                                                                      <  1:53569/250‑1 (MQ=255)                                                                                           gTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTgg                                                                                                                                                 >  1:25088/1‑250 (MQ=255)                                                                                                tttttGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGAtctc                                                                                                                                            <  1:45254/250‑1 (MQ=255)                                                                                                 ttttGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGAtctcct                                                                                                                                          >  1:335690/1‑249 (MQ=255)                                                                                                  tttGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTACTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcatc                                                                                                                                         >  1:404631/1‑251 (MQ=255)                                                                                                    tGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCtcatca                                                                                                                                        >  2:261438/1‑250 (MQ=255)                                                                                                     gCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCACGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAAc                                                                                                                                      >  2:359807/1‑251 (MQ=255)                                                                                                        aGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACTGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGtt                                                                                                                                   >  1:116303/1‑251 (MQ=255)                                                                                                               cGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATc                                                                                                                            <  2:111345/251‑1 (MQ=255)                                                                                                                ggCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCg                                                                                                                           >  2:141655/1‑251 (MQ=255)                                                                                                                ggCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCg                                                                                                                           >  1:45782/1‑251 (MQ=255)                                                                                                                    gCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTg                                                                                                                       <  2:40522/251‑1 (MQ=255)                                                                                                                                 tGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATg                                                                                                          >  1:75327/1‑251 (MQ=255)                                                                                                                                     tCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTg                                                                                                       <  1:104866/250‑1 (MQ=255)                                                                                                                                         ccGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTa                                                                                                   >  2:101123/1‑250 (MQ=255)                                                                                                                                             aaTGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTg                                                                                              >  2:259125/1‑251 (MQ=255)                                                                                                                                                gggTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGCGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGAGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATg                                                                                           >  2:150987/1‑251 (MQ=255)                                                                                                                                                  gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGAc                                                                                                                         <  1:5520/219‑1 (MQ=255)                                                                                                                                                  gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGAc                                                                                                                         >  2:5520/1‑219 (MQ=255)                                                                                                                                                    cACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTaa                                                                                       <  1:272060/251‑1 (MQ=255)                                                                                                                                                    cACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTa                                                                                        <  2:176701/250‑1 (MQ=255)                                                                                                                                                         ccAGCCCAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGTCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCACCGTTGACGATCGCCTGACCTTCAATAATGGTGCGTAAAGTGATGTTAAATAcc                                                                                  >  2:431050/1‑251 (MQ=255)                                                                                                                                                                     ctcCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGCTAGGCCTAACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGGGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTcaccac                                                                       >  2:311043/1‑250 (MQ=255)                                                                                                                                                                                aacaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGgcgc                                                           >  2:427591/1‑251 (MQ=255)                                                                                                                                                                                            gACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGc                                               >  2:92248/1‑251 (MQ=255)                                                                                                                                                                                                  atcatGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGACAACTc                                         >  1:127883/1‑251 (MQ=255)                                                                                                                                                                                                                     gATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACTTCAGCGCGGATGGCATTTGATCCTCCGGCTCCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGTACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGATGCGGAAAGTGATGTTAAATACCTAATTCACCACATCCTGCGGCGCTTCATCAAAAGCTAACGCGGCGGTCAATACCGGTTcc                      <  2:351063/251‑2 (MQ=255)                                                                                                                                                                                                                                 aGCGTACGCCAATGGGCAAGAACTTGTGCTGTTCATCAGCACAGAGAGCATTTTATCCTCCGGGACCGTACCAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTTACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGTCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGaaaaa          <  2:33781/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccAATGGGCAAGAACTTGTGCTTTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCtgc  <  2:94938/251‑3 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     CGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAATAATCTTC  >  NC_000913/4312260‑4312742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑