Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,640,045	+C	coding (13/534 nt)	rrrQ ←	putative lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,640,045	1	.	C	98.1%	195.6 / ‑3.0	53	coding (13/534 nt)	rrrQ	putative lysozyme
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (22/30);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑T‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCG  >  NC_000913/1639795‑1640278                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          cATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑t                                                                                                                                                                                                                                            <  1:444083/251‑1 (MQ=255)  aTGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑tc                                                                                                                                                                                                                                           >  1:102414/1‑250 (MQ=255)      cAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑t‑ctttt                                                                                                                                                                                                                                      >  1:284375/1‑250 (MQ=255)       aGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTtg                                                                                                                                                                                                                                      >  2:10947/1‑251 (MQ=255)              tATCTCGCTCAAGGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTc                                                                                                                                                                                                                                 <  2:332208/249‑1 (MQ=255)               aTCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAc                                                                                                                                                                                                                               <  1:208374‑M1/250‑2 (MQ=255)                  ccgCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCCCTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGACC‑TTTTGTGTTCAcccgc                                                                                                                                                                                                                           <  2:316232‑M1/250‑6 (MQ=255)                             cGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtct                                                                                                                                                                                                                <  1:400295‑M1/251‑17 (MQ=255)                             cGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCACCCCCCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGTTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCTcccgcccgcttcgtct                                                                                                                                                                                                                <  2:200000‑M1/251‑17 (MQ=255)                                 gACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacg                                                                                                                                                                                                            >  1:334213‑M1/1‑231 (MQ=255)                                   ccTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgat                                                                                                                                                                                                          <  2:437245‑M1/251‑23 (MQ=255)                                              ttttCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcata                                                                                                                                                                                                >  2:217651‑M1/1‑218 (MQ=255)                                                  ccTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTCTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaact                                                                                                                                                                                            <  2:242185‑M1/250‑37 (MQ=255)                                                       gACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttggga                                                                                                                                                                                      <  1:288247‑M1/251‑43 (MQ=255)                                                               cATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatact                                                                                                                                                                               <  2:87900‑M1/250‑50 (MQ=255)                                                                   ttGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagc                                                                                                                                                                          >  2:198728‑M1/1‑197 (MQ=255)                                                                     gtgaaAACCGGTTTTTACAGCCACATCTGTGGCACCCCGACAGCTGTTTCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGGAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCTTATCTGA‑TCTTTTGTGTTCTcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctc                                                                                                                                                                        <  2:3771‑M1/249‑57 (MQ=255)                                                                      ggAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcc                                                                                                                                                                       <  1:149860‑M1/251‑58 (MQ=255)                                                                       gAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgccagcttcgtcttacgatattcataataaactttgggatcatactgaagctccc                                                                                                                                                                      >  2:432010‑M1/1‑193 (MQ=255)                                                                        aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgctattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                     >  2:73259‑M1/1‑192 (MQ=255)                                                                        aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                     <  1:339632‑M1/251‑60 (MQ=255)                                                                        aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                     >  2:259253‑M1/1‑192 (MQ=255)                                                                        aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                     <  1:229954‑M1/251‑60 (MQ=255)                                                                        aaaaaCGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctcccc                                                                                                                                                                     >  2:139965‑M1/1‑192 (MQ=255)                                                                                      catccaCCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcct                                                                                                                                                       >  1:214163‑M1/1‑178 (MQ=255)                                                                                         ccaccaCTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgt                                                                                                                                                     <  1:61444‑M1/250‑76 (MQ=255)                                                                                           accacTGTGGCACCCCGACAGATGGTCCCTATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCATTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAAAGGCAGCCGACAGGCCGTATCTGG‑TCTTTTGTGTTCAcccgcccgcttcgtctcacgatatgcatcatcaactttaggatcatactgaggctcccctccagatccctcctgtagt                                                                                                                                                  >  2:166150‑M1/1‑173 (MQ=255)                                                                                            ccacTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagac                                                                                                                                                 <  1:330113‑M1/251‑80 (MQ=255)                                                                                                 gtgGCACCCCGACAGATGGTCCATATGCAAGAACCATCTCGGTATGCCATTGTGTGGTTACCGTCTTTTTCGTCCAGAAACTGGTCAAGTAGCTGAGGAGCAGATGCGCCAGCCCCAACCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGGGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatagtgaagctccccgccagatgcctcctgtagacgcatc                                                                                                                                            <  2:131111‑M1/251‑85 (MQ=255)                                                                                                               gATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcggg                                                                                                                              <  2:388217‑M1/251‑99 (MQ=255)                                                                                                                   gTCCATATGCCAGAACCATCGCGGTCTGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcctcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatctcgcgaccttcgggaact                                                                                                                          >  2:19344‑M1/1‑149 (MQ=255)                                                                                                                     ccATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTAAAGTAGCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaa                                                                                                                        <  2:167845‑M1/251‑105 (MQ=255)                                                                                                                             cAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctt                                                                                                                <  1:435678‑M1/251‑113 (MQ=255)                                                                                                                                 aCCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttcca                                                                                                            <  1:217651‑M1/251‑117 (MQ=255)                                                                                                                                         ggTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcatcttacgatattcatcataaactttgggatcatcctgcagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataa                                                                                                     >  2:363962‑M1/1‑127 (MQ=255)                                                                                                                                            aTGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcg                                                                                                 >  1:334844‑M1/1‑124 (MQ=255)                                                                                                                                                             ccTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACAAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacct                                                                                <  2:454846‑M1/251‑145 (MQ=255)                                                                                                                                                             ccTTATTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGAGTTAAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgacagatgcctactgtagacgcatcgcgcgaccttcgggaactaaatcccctttcaagctataaagcgaagccaaacgaatacct                                                                                >  2:242660‑M1/1‑107 (MQ=255)                                                                                                                                                                   ttttCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgct                                                                             <  1:374763‑M1/248‑148 (MQ=255)                                                                                                                                                                            aGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaagcccctttccagctataaagcgaagccaaacgaataccagctgcttgtgcaagt                                                                 >  2:231242‑M1/1‑92 (MQ=255)                                                                                                                                                                              aaaCTGGTCAAGTATCTGAGGAGCAGAGGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcgtcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacccgctgcttgtgcaagttt                                                               <  1:94360‑M1/251‑162 (MQ=255)                                                                                                                                                                               aaCTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGAAAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaacttttggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaactaatacctgctgcttgtgcaagtttt                                                              <  1:42854‑M1/251‑163 (MQ=255)                                                                                                                                                                                    gTCAAGTATCTGAGGAGCAGATTCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttt                                                         <  2:334844‑M1/251‑168 (MQ=255)                                                                                                                                                                                        aGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttga                                                      >  1:244247‑M1/1‑80 (MQ=255)                                                                                                                                                                                         gTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgtccggcatctaagaatgcgcggcg                                                    >  1:399271‑M1/1‑79 (MQ=255)                                                                                                                                                                                               gaggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaa                                                <  1:106087‑M1/249‑177 (MQ=255)                                                                                                                                                                                                aggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaat                                              >  1:174478‑M1/1‑72 (MQ=255)                                                                                                                                                                                                 ggagCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatac                                            >  2:248599‑M1/1‑71 (MQ=255)                                                                                                                                                                                                     cAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgacgccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaca                                         >  2:176473‑M1/1‑67 (MQ=255)                                                                                                                                                                                                                gCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTTTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagtt                             <  2:174478‑M1/251‑196 (MQ=255)                                                                                                                                                                                                                 cACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttt                            >  1:15419‑M1/1‑55 (MQ=255)                                                                                                                                                                                                                     aaTCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatccaaaagagcgtcagttttaag                        <  1:63857‑M1/251‑201 (MQ=255)                                                                                                                                                                                                                                gAACGGCAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaaca             <  2:384313‑M1/251‑212 (MQ=255)                                                                                                                                                                                                                                      cAGCCGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacaccttta       <  2:61419‑M1/251‑218 (MQ=255)                                                                                                                                                                                                                                          cGACAGGCCGTATCTGA‑TCTTTTGTGTTCAcccgcccgcttcgtcttacgatattcatcataaactttgggatcatactgaagctccccgccagatgcctcctgtagacgcatcgcgcgaccttcgggaactaaatcccctttccagctataaagcgaagccaaacgaatacctgctgcttgtgcaagttttgtttttgaaccgaaatacaaaagagcgtcagttttaagcatttaaaacacctttattgt   <  2:171743‑M1/251‑222 (MQ=255)                                                                                                                                                                                                                                           gACAGGCCGTATCTGG‑TCTTTTGTGTTCAcccgccagcttcgtcttacgatattcatcataaactttgggagcatactgatgctccccgccagatgcctcctgtagacgcatcgcgcgaccttccggaactaaatcccctttcaagctataacgcgaagccaaacgaataccagctgcttgtgcaagttttgtttttgcacagaaatacaataaagcgtcagttttaagcatttataccacctttattgtt  >  2:169883‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          CATGCCAGCGCCTTATCACGCTCAATGGCGTTGACCTGGTCGCATTTTTCCTTCGACAGTTTCATATTGGGAAAAACGGTTTTTCCATCCACCACTGTGGCACCCCGACAGATGGTCCATATGCCAGAACCATCGCGGTATGCCATTGTGTGGTTACCTTCTTTTTCGTCCAGAAACTGGTCAAGTATCTGAGGAGCAGATGCGCCAGCACCAATCAGCGCCAGAACGGCAGCCGACAGGCCGTATCTGA‑T‑TTTTGTGTTCATAGATATTTATGATGAGGACGCTCGTGCTTATTGGCAGGATTTTCAATCTTAAAGGAGTACTGATGCTGCAGATAAGACTCAACTTTTTCTGACAATTTTTCTGCTACTTCCAGGAAGACTTGCCGGACGCTCCTTCTGGCTGCTGCCTCATAAAACTCCAGCGCAGCTCCTTCAACACGGTCCATGGCGACATCCAGGTCAAAAATTTCACCGTCAAAGCG  >  NC_000913/1639795‑1640278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑