Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,985,816	T→C	intergenic (‑224/‑31)	yqeF ← / → yqeG	putative acyltransferase/putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,985,816	0	T	C	100.0%	121.2 / NA	32	intergenic (‑224/‑31)	yqeF/yqeG	putative acyltransferase/putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

CCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAG  >  NC_000913/2985569‑2986060                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     cccGACAATCACAACGTCTTTCATCACATCCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCATCCTCTAACCCACCAATAGATGTGTAGATAGTGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCGCCATTCTGt                                                                                                                                                                                                                                                   <  1:85215/251‑1 (MQ=255)       aaTCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAAtt                                                                                                                                                                                                                                             <  1:470763/251‑1 (MQ=255)                    ttCATCACAACCACGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGATCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAgatgat                                                                                                                                                                                                                                   <  2:337204/248‑1 (MQ=255)                     tcatcaCAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGt                                                                                                                                                                                                                               >  1:124760/1‑251 (MQ=255)                                            aCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGAGACGTTCtaaaaaaaccccgacatttgc                                                                                                                                                                                                        <  1:348097‑M1/251‑22 (MQ=255)                                                  cAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggt                                                                                                                                                                                                  <  2:472060‑M1/251‑28 (MQ=255)                                                         aaaTGATTGTTAATTACAGTTATATTAATTGGTTAAAACAAGAGATTTTTATCAAAAAGGAAATATGCCTGAGCAGCAGTCCGAGACATAACTGGCACGTAGGGTTTTCAACCACTAACACACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaacaccgacatttgccggggttgtgagc                                                                                                                                                                                           >  2:420989‑M1/1‑217 (MQ=255)                                                                  ttAATTACAGTTATCTTAATTTTTTAAAAAAAGTGATTTTTATCACAAATGAAATATGCCTGAGCATCATTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGTCCTCCATGCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggtgttgtgagcataacgta                                                                                                                                                                                   <  1:263324‑M1/250‑43 (MQ=255)                                                                   tAATTACAGTTATCTTAATTGTTTAAAAAACGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaat                                                                                                                                                                                 >  1:316229‑M1/1‑207 (MQ=255)                                                                        aCAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgctt                                                                                                                                                                             >  1:191014‑M1/1‑202 (MQ=255)                                                                         cAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttat                                                                                                                                                                           >  1:32491‑M1/1‑201 (MQ=255)                                                                             tATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttatttt                                                                                                                                                                        >  2:400545‑M1/1‑197 (MQ=255)                                                                                   aaTTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggac                                                                                                                                                                 <  1:330421‑M1/251‑61 (MQ=255)                                                                                         ttAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatc                                                                                                                                                            <  1:186449‑M1/250‑66 (MQ=255)                                                                                          tAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgc                                                                                                                                                          >  1:377831‑M1/1‑184 (MQ=255)                                                                                                     aTTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgaaatgcttattttaccggacgcatcgcagggaacaga                                                                                                                                                >  1:485562‑M1/1‑173 (MQ=255)                                                                                                           aTCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgt                                                                                                                                         >  1:465371‑M1/1‑167 (MQ=255)                                                                                                                  aGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggat                                                                                                                                  <  1:141605‑M1/251‑92 (MQ=255)                                                                                                                   ggAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatg                                                                                                                                 >  1:80080‑M1/1‑159 (MQ=255)                                                                                                                            ccTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctg                                                                                                                        >  2:178889‑M1/1‑150 (MQ=255)                                                                                                                             cTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgt                                                                                                                       >  1:295448‑M1/1‑149 (MQ=255)                                                                                                                                             gaCATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattac                                                                                                       >  1:225077‑M1/1‑133 (MQ=255)                                                                                                                                                         aCGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggaatggctgttggagaacagcattaccatacggtcgat                                                                                           >  2:84686‑M1/1‑121 (MQ=255)                                                                                                                                                                                              gTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgt                                                      >  1:270697‑M1/1‑84 (MQ=255)                                                                                                                                                                                                           tAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtg                                          >  1:435329‑M1/1‑71 (MQ=255)                                                                                                                                                                                                                 cccATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaa                                    <  2:465371‑M1/250‑186 (MQ=255)                                                                                                                                                                                                                         tAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatc                            <  1:304345‑M1/250‑194 (MQ=255)                                                                                                                                                                                                                            ccTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtag                        <  1:286603‑M1/251‑198 (MQ=255)                                                                                                                                                                                                                                 tttATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacat                   >  1:135695‑M1/1‑49 (MQ=255)                                                                                                                                                                                                                                    aTGGTAAATTGCCCTCCAATCTGTTGAATTTGTAGATGATACGTTCtaaaaaaaccccgacaaatgccgggggtgtgagcataacgtaatgctgattttaccggacgcatcgccgggaacagaataacgacgcggatggtatggctgttggtgaacagcattcccatacggtcgatcccaattcccagacctgctgtgggcggtaagacatggaccagttcggtgccgtaatcttcagcgtagaacatcg                 <  2:324244‑M1/250‑205 (MQ=255)                                                                                                                                                                                                                                                ccTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctggtggtgaaaagcattaccagacgggcgataccaattcccagacctgctgtcggcggtaacccattatccagtgcggggacgatcacttcaccgtagaaaatagcttcctcgtctcc    >  2:388746‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                                                  cccaTTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccatacggtcgataccaattcccagacctgctgtcggcggtaagccatgttccagtgcggtgacgtaatcttcatcgtagaacatcgcttcgtcgtcacctg  <  2:407887‑M1/250‑220 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     CCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTATTACTGCTCTGGTCGCCTGGCCTTTAACATATTGGCCACATAAAGCCTTGTGCCAGTTCATCCTCTCATCAAAAACATCAGCAGGTGAAG  >  NC_000913/2985569‑2986060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑