Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,730,870	T→C	noncoding (288/1542 nt)	rrsG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,730,870	0	T	C	95.6%	87.7 / ‑7.1	46	noncoding (288/1542 nt)	rrsG	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (19/24);  minor base A (0/2);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCG  >  NC_000913/2730712‑2731095                                                                                                                                                                |                                                                                                                                                                                                                                  cccGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGCTCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCtt                                                                                                                                       <  2:98759/251‑1 (MQ=14)  ccGCGGAAAGTACTTTACAACCCGAAGGCCTTCTGCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATGGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑TGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCATATCCGATGGCGAGAGGCCCGAATGTCCCCCTCttt                                                                                                                                      <  2:394718/251‑1 (MQ=11)   cgttGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGACTTGCGCCCATTGTGCAATATTCACCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGAGCGTCGCCTTGGTGAGCCGTTACCCCACCAACCAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAAGTCACCctccttt                                                                                                                                     >  2:373811/4‑247 (MQ=11)      tGAAAGTCCTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTc                                                                                                                                  >  1:69633/1‑251 (MQ=14)       gAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCt                                                                                                                                 <  1:326133/251‑1 (MQ=14)        aaaGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGCAGGTCCCCcttcttgtactt                                                                                                                                >  2:487458/1‑241 (MQ=9)            gaCTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGa                                                                                                                            <  1:158942/250‑1 (MQ=14)              cTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGAc                                                                                                                           >  1:230038/1‑250 (MQ=14)               tttACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACAt                                                                                                                         >  1:28957/1‑251 (MQ=17)                         gAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTAt                                                                                                               >  2:187567/1‑251 (MQ=18)                         gAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCCCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGACCCGAAGGTCCCCCTCTATGGACTTGCGCAATTATGCGGTAt                                                                                                               >  2:489167/1‑251 (MQ=11)                                 tcttcATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAcc                                                                                                       >  2:242819/1‑251 (MQ=18)                                 tcttcATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAc                                                                                                        <  1:187567/250‑1 (MQ=18)                                    tcATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTAcc                                                                                                       <  1:37698/248‑1 (MQ=18)                                    tcATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCg                                                                                                      <  1:493230/249‑1 (MQ=18)                                     cATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGTTCTTGCGACATTATGCGGTATTAGCTACCGttt                                                                                                   <  1:98266/251‑1 (MQ=18)                                          ccgcgGCATGGCTGCATCTGTCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGGTTGGCTGGTCATCCGCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCACCAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCagt                                                                                              <  2:245813/250‑1 (MQ=11)                                                           cAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATc                                                                             <  1:436474/251‑1 (MQ=18)                                                              gCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAgg                                                                          <  1:179119/251‑1 (MQ=33)                                                                    gcCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCtt                                                                    <  1:196674/251‑1 (MQ=21)                                                                     cccATTTTGCAATATTCCCCGCTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCGGTTCCAGTGTGGCTAGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCGTGGTTAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGTAGGTCCCCCTCTTTTGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCACCATCAGTCAGCTTc                                                                   <  2:294526/251‑1 (MQ=11)                                                                      ccagtgtgaaattttccccctggcggcCTCCCGTAGGAGTCGGGACCGTGTCTCAGTTCCGGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc                                                                  <  2:149311/226‑1 (MQ=11)                                                                         ttGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTccc                                                                 >  1:504561/1‑249 (MQ=25)                                                                                  attccccttcgctgcCTCCAGTAGTAGCCTGGACCGTGTCTCCTGGCCAGTGTGGCTGGTCGTCCTCGC‑CGGCCAGCCAGGGATCGTCGTCATGGTCAGCCCTTCGCCCACCAACAAGCAAACCCGCTCTGGGCACATCCGATGGCTAGAGGCCCAAGGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCGGTAGTTATTCCCGTCCATCAGGCATCTTCCAAGTCATTAc                                                        <  2:289481/239‑1 (MQ=255)                                                                                    tCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCAc                                                    >  1:314113/1‑251 (MQ=21)                                                                                       ccACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCg                                                 <  1:46509/251‑1 (MQ=21)                                                                                              tgcCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCGCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGcc                                           >  1:398459/1‑250 (MQ=25)                                                                                               gcCTCACGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCATTGGTGAGCCGTTACCCCACCAACCAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGCAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCCGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACCTTACTCACCCGTCCGCCAc                                         >  2:203170/1‑251 (MQ=11)                                                                                                 cgcccttAGGAGTTTGGACCGTGCCTCATTTCCAGTG‑GGGTGGTTCTGCCCACCAGACCAGCCAGGGATCGCCGCCTTGGTTAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGTCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCTGGATAATCTTCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTCCCCAGCCATTTCCCTCCCGTCCGCCAcgc                                       <  2:69633/245‑3 (MQ=2)                                                                                                  tCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCCCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCg                                      >  1:295376/1‑251 (MQ=21)                                                                                                    cgcgaggtctctagACCGTGTCTCCGCTCCAGTGTGGCTGGTCCTCCTCCC‑ACACCAGCCAGGGATGTTCGCCGTGGTGAGCCGTTTCCCACCCAACACTCTAACCCCATCTGGGCTCATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGTCATTAGGCGGTATTAGCTAGCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGCCATTACTCCCCCGTCCGCCACCCGTc                                    <  2:307357/238‑1 (MQ=2)                                                                                                        aGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGCGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGACCGACGGTCCCCCTCTTTGGCCTTGCGACATTATGCGGTATGAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCACAGACATTACTCACCCGTCCGCCCCACGTCAGCg                                >  2:468370/1‑251 (MQ=11)                                                                                                          gagtccggccagtgtCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGAGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGAAATTACTCACCCGTACGCCCCTCGTCAGCGa                               >  2:147770/12‑250 (MQ=11)                                                                                                           aGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGa                               >  1:22374/1‑249 (MQ=25)                                                                                                              cTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc                                                                                                                              >  1:503659/1‑151 (MQ=17)                                                                                                              cTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGc                                                                                                                              <  2:503659/151‑1 (MQ=17)                                                                                                                 gACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGTTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCaa                       <  1:207074/251‑1 (MQ=37)                                                                                                                    ggtgtCTCAGTTCCATTGTGGCTGGTCATCCCCTC‑AGACAAGCAAGGGATCGTCGCCTTGGTGAGCCGTTACCCCCCCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgct                    <  2:422414/250‑1 (MQ=255)                                                                                                                    cGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGCCCAGCCAGGGATCGTCGCCTTGGTGACCCGTTGCCCCACCAACATGCTAACCCCTTCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTAGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgct                    <  1:251962/251‑1 (MQ=11)                                                                                                                    cGTGGCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGCCCAGCCAGGGTGCGTCTCCTTGGGGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgct                    <  1:209090/251‑1 (MQ=11)                                                                                                                           cgtgtcctgtgtggtgggtccaccTCTC‑AGACCGGCAAGGGATCGTCGCCTGGGTGGGCCGTTACCCCACCAACAAGCTAATCCCTTCGGGGCACATCCGATGGCAGGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACGCGTCAGCGAAGCAGCAAGCTGCTTc               <  2:295376/227‑1 (MQ=14)                                                                                                                                   gtgtGGCGGGTCATCCTCTC‑AACCCAGCCTGGGACCGTCCCCTTGGTGAGCCGTTACCCCACAAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTACCCCCCTCCATCAGGCAGCTTCCCAGCCATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTGAcc       <  2:424919/249‑1 (MQ=14)                                                                                                                                    tgtgGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGtt    >  1:221322/1‑251 (MQ=38)                                                                                                                                     gtgGCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTCCCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc   <  1:203170/251‑1 (MQ=255)                                                                                                                                      tggctggacctcccacc‑AGACCAGCCAGGGATCGTCGCCTGGGTGAGCCTTTCCCCCACCAAAAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTc   <  2:59931/234‑1 (MQ=14)                                                                                                                                       ggCTGGTCATCCTCTC‑AGACCAGCCAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTACAGTAGTTATCCCCCTCCATCAGACAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCg  >  1:286429/1‑250 (MQ=255)                                                                                                                                                                |                                                                                                                                                                                                                                  CCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTC‑AGACCAGCTAGGGATCGTCGCCTTGGTGAGCCGTTACCCCACCAACAAGCTAATCCCATCTGGGCACATCCGATGGCAAGAGGCCCGAAGGTCCCCCTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCG  >  NC_000913/2730712‑2731095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑