Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,795	G→A	Q128* (CAG→TAG)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,795	0	G	A	100.0%	41.5 / NA	14	Q128* (CAG→TAG)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/2);  total (12/2)

AGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCC  >  NC_000913/2481577‑2482033                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               aGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGAGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAAcc                                                                                                                                                                                                                >  1:91302/1‑251 (MQ=255)                  cTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTaaa                                                                                                                                                                                                >  1:110622/1‑250 (MQ=255)                      aagtaagGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTCAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCACCGCCATTAACCCCTGAACGACTCTAAAAAat                                                                                                                                                                                            >  2:84925/1‑250 (MQ=255)                               aaaacataagtgcAGGACTATAACCCCCAGAGGAACATTAATTAAAAACACCCACCCACAGCAAAATTTATCAAAAATATAACCCCCAAATATCGCCCCACATATCGGAGATATAATCACGGTCATTCACCATAATGCCAGAAAAATTTTTCTTTTTTCGGGCGAATAATTCCTAAATAAAACCCCCTAAGACAGAAAAATTAGCGCCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACAt                                                                                                                                                                                  <  2:173138/239‑1 (MQ=255)                                               aCGATAATCCACATAGGGACATTGATTAAACATATCCAACCCCAACTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGAGATAATCACGGTCATAGACCATAATGCAAGAGCAAATGATCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACAGCCCCGCCATTAACCCCTGAAAGACTCTAAAAAATATCAGCACATCAAGATTGGTCGAt                                                                                                                                                                    >  2:399622/1‑249 (MQ=255)                                                                                    aaCCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGAc                                                                                                                              >  1:25747/1‑250 (MQ=255)                                                                                                                            cGGCCCACATATGGGAGCTAAAATCCCGTTCATTGACCATAATGCAAGAAAAATTGTTTTTTTTTCGGGCGAATAATTCCTTAAAAAAAACCTCTAGGACATTGAAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCa                                                                                     <  1:84925/251‑1 (MQ=255)                                                                                                                              gCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAAc                                                                                   >  1:20713/1‑251 (MQ=255)                                                                                                                                              gctAATCACCGTCATTGACCATAATGCCAGCGCAACTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCCATGACAGCGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAACTATCAACACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGACAGTAACAggg                                                                    >  2:453588/3‑250 (MQ=255)                                                                                                                                                                                 aTGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGAt                                 >  2:473927/1‑250 (MQ=255)                                                                                                                                                                                    ttCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAAcc                             >  1:216827/1‑251 (MQ=255)                                                                                                                                                                                           ttCTGGCGGATAATTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCa                           >  2:71153/1‑246 (MQ=255)                                                                                                                                                                                                      aaTTCCTTAATAATAAACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACACCTGAACGACTCTAAAAAATATCAGCACATCAAGCTTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGAGATAACCCAGGTGCCTTCGTCTGtt           >  2:105755/1‑251 (MQ=255)                                                                                                                                                                                                               ataataaACTCTATGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGAGATAACCCAGGTGCCTTCGTCTGTTGATGCTccc  >  2:447479/1‑251 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               AGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTGGAATTAACGGCCCCGCCATTAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCC  >  NC_000913/2481577‑2482033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑