Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,638,571	G→A	H33Y (CAT→TAT)	cspI ←	cold shock‑like protein CspI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,638,571	0	G	A	100.0%	65.2 / NA	21	H33Y (CAT→TAT)	cspI	cold shock‑like protein CspI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

CATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACA  >  NC_000913/1638325‑1638806                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             atcaataccctggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGAGTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGAc                                                                                                                                                                                                                                           <  2:387720‑M2/108‑1 (MQ=255)   caataccctggaaatgatgccggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACaaa                                                                                                                                                                                                                                        <  1:160885‑M2/111‑1 (MQ=255)                       cggttacccaaagctatgccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACCCATCTTTTCTGCCATCtt                                                                                                                                                                                                                    <  2:76914‑M2/131‑1 (MQ=255)                                        gccggactgaatgtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCGTTGCTGCCATCTTTCGGCGTGATGAAACCa                                                                                                                                                                                                   <  1:149686‑M2/148‑1 (MQ=255)                                                    gtccaccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTc                                                                                                                                                                                        <  1:322443‑M2/159‑1 (MQ=255)                                                       caccgtcagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTCTTCAggg                                                                                                                                                                                    <  1:246743‑M2/163‑1 (MQ=255)                                                             cagtaatttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAAc                                                                                                                                                                              >  2:83937‑M2/83‑251 (MQ=255)                                                               ggaatgtttatacccggcgtcactgccggggttttgcttgtcacaaaaaagtcgtagactcatgcagttcacccactcacTATATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCACAGTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGTATTGCTGAGAAATAGACAATCACATCTTTGCTGCGTTCTTTCGGCGTGATGATACCAAAACCTTTTGCAGGGTTAAACCa                                                                                                                                                                            <  2:259268‑M2/171‑1 (MQ=255)                                                                  atttttatacccggcgtaactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCAttt                                                                                                                                                                         <  1:254267‑M2/174‑1 (MQ=255)                                                                                   aactgccgggttattgcttgtcacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCAtt                                                                                                                                                         <  1:284302‑M2/190‑1 (MQ=255)                                                                                                        cacaaaaaagtggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAAACTTTTTCAGGGTTAAACCATTTCCCTAAACCAGTCATTTTGTTAGACATAATTATTACCt                                                                                                                                   >  2:212920‑M2/40‑251 (MQ=255)                                                                                                             aaaagaggtagactcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg                                                                                                                               >  2:419171‑M2/35‑250 (MQ=255)                                                                                                                          tcatgcagttaacccactcacTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt                                                                                                                 >  1:419609‑M2/22‑251 (MQ=255)                                                                                                                                            cACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGa                                                                                                <  1:419171/250‑1 (MQ=255)                                                                                                                                                      aCAGCGGCAGCAACTTTAGGTCCGTTCTAAATACCAAATTCCACTTCATGATTCGCAGTTAATGTTTTGAAATCGTTGCCCCGAATTGCTGAGAAATAGACAAACACATCTTTTCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGCTAAACCATTTCACTAAACCAGTCCTTTTGTTAGACATACTTATTACCTTTTGAAGAAATTAACCCTTGGGCAGAATGGTCCGATAAAAAATATc                                                                                     >  2:436483/1‑251 (MQ=255)                                                                                                                                                                                                           ctcAGTTAATGTTTTGAAATAGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGGTGAAAACAAAACCTTTTGCAGGGTTAAACCATTTCACTAAACCAGACATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAAAAAATCGCAAAAGGAACAAATAATAAAATtata                                >  2:177151/1‑250 (MQ=255)                                                                                                                                                                                                                  aacgttttgaatccgtgtctctGCATGGCTGAGAAAAAGACAAAAACAACTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTCTTTCAGGTTTAAACCTTTTCACTAAACCAGTCATTTTGTTAGACATAATTAATACCTTTTGAAGAAATTAGCCCTTGAGCAGAATGGTCCGAAAAAAAATAAAAGAGAGTAAAACCAA‑CAAGGAAATCTCAAGATGTACAAACTATAAAATTATAACAATGa                         <  2:334245/232‑1 (MQ=255)                                                                                                                                                                                                                  aaTGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATAAGAGA‑AAAAACCAAACAAGGAAATCTAAAGAGGAAAAAATAAAAAAATTATaaaaagaac                        >  1:26730/1‑244 (MQ=255)                                                                                                                                                                                                                     gTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGCGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAGCCAGTCATTTTGGTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAGGAAAGCGCAAGAGGTACAAATAATAAAATTATAACAATGACTg                      <  2:453876/250‑1 (MQ=255)                                                                                                                                                                                                                        ttGAAATCGTTGCTCTGAATTGCTGAGAAATAGACAAACACATCGTTGCTGCCATCTTTCGGCGTGATGAAACCAAATCCTTTTTCAGTGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCtt                   <  2:471803/250‑1 (MQ=255)                                                                                                                                                                                                                                         aaTTGCTGAGAAATAGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGCAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACa  >  2:396215/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             CATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGTTTGTAATATTGTCTACCTCAAAGCGCCACTACATGAACAGCGGCAGGACCTTTAGGTCCGTTCTCAATACCAAATTCAACTTCCTGATTCTCAGTTAATGTTTTGAAATCGTTGCTCTGAATTGCTGAGAAATGGACAAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAA‑CAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACA  >  NC_000913/1638325‑1638806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑