Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,332	T→C	intergenic (+69/‑20)	ydfG → / → rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,332	0	T	C	100.0%	103.3 / NA	31	intergenic (+69/‑20)	ydfG/rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/21);  total (10/21)

AAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGTGGCAG  >  NC_000913/1628087‑1628569                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aaaGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAacccact                                                                                                                                                                                                                                           <  2:259737/250‑1 (MQ=255)     gCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACta                                                                                                                                                                                                                                      <  2:241970‑M1/251‑3 (MQ=255)              gcaaagcAGAAAAAACCTATTAAAATACCGTTGCATTGACGCAAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGCCTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGGAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGCCTCATTCAGTTAAACCACTCACtatatgaaca                                                                                                                                                                                                                              >  2:348990‑M1/3‑240 (MQ=255)                     aGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcagg                                                                                                                                                                                                                      <  2:53812‑M1/251‑19 (MQ=255)                       aaaaaaCCTATCAAAATACCGGTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACTTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggac                                                                                                                                                                                                                    <  2:368463‑M1/251‑21 (MQ=255)                          aaaCCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGATGCCGTCTGGTGGGTGGCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGTACTGAATGTACACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCTCtacatgaacagcggcaggtcctt                                                                                                                                                                                                                 <  2:291553‑M1/251‑24 (MQ=255)                                    aaaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgtt                                                                                                                                                                                                       >  1:387720‑M1/1‑218 (MQ=255)                                     aaTACCGGTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttc                                                                                                                                                                                                      <  2:152433‑M1/251‑35 (MQ=255)                                                aTTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaa                                                                                                                                                                                           <  1:72725‑M1/251‑46 (MQ=255)                                                  tGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaat                                                                                                                                                                                          <  1:348990‑M1/250‑47 (MQ=255)                                                              aTGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgat                                                                                                                                                                             >  2:261093‑M1/1‑192 (MQ=255)                                                                gTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgatctcaataccaaattcaacttcctgattc                                                                                                                                                                           >  2:322443‑M1/1‑190 (MQ=255)                                                                         gCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaat                                                                                                                                                                  <  1:25827‑M1/251‑71 (MQ=255)                                                                           cGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgt                                                                                                                                                                <  1:171199‑M1/251‑73 (MQ=255)                                                                            gTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggcccgttctcaataccaaattcaacttcctgattctcagttaatgtt                                                                                                                                                               >  1:453876‑M1/1‑178 (MQ=255)                                                                                ggtgggtgTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttg                                                                                                                                                            <  1:478648‑M1/250‑77 (MQ=255)                                                                                            cGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctct                                                                                                                                               <  1:261093‑M1/251‑90 (MQ=255)                                                                                                      tCACGTCAATATCCATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaaattcctgattctcagttaatgttttgaaattgttgctctgaattgctg                                                                                                                                      >  2:371620‑M1/1‑152 (MQ=255)                                                                                                              atatcaataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCTTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaataga                                                                                                                             <  2:310351‑M1/251‑108 (MQ=255)                                                                                                                atcaataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgagtctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagac                                                                                                                            <  2:387720‑M1/250‑109 (MQ=255)                                                                                                                  caataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaa                                                                                                                         <  1:160885‑M1/251‑112 (MQ=255)                                                                                                                                      cGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacccatcttttctgccatctt                                                                                                     <  2:76914‑M1/251‑132 (MQ=255)                                                                                                                                                       gCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatcgttgctgccatctttcggcgtgatgaaacca                                                                                    <  1:149686‑M1/251‑149 (MQ=255)                                                                                                                                                                   gTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttc                                                                         <  1:322443‑M1/250‑160 (MQ=255)                                                                                                                                                                      cACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaaccttcttcaggg                                                                     <  1:246743‑M1/251‑164 (MQ=255)                                                                                                                                                                            cAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaac                                                               >  2:83937‑M1/1‑82 (MQ=255)                                                                                                                                                                              ggaatgtttatacccGGCGTCACTGCCGGGGTTTTGCTTGTCACAAAAAAGTCGTAGACTCATGCAGTTCACCCACTCACtatatgaacagcggcaggacctttaggtccgttctcaataccacagtcaacttcctgattctcagttaatgttttgaaatcgttgctctgtattgctgagaaatagacaatcacatctttgctgcgttctttcggcgtgatgataccaaaaccttttgcagggttaaacca                                                             <  2:259268‑M1/245‑172 (MQ=255)                                                                                                                                                                                 aTTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccattt                                                          <  1:254267‑M1/251‑175 (MQ=255)                                                                                                                                                                                                  aaCTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcatt                                          <  1:284302‑M1/250‑191 (MQ=255)                                                                                                                                                                                                                       cacaAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaaactttttcagggttaaaccatttccctaaaccagtcattttgttagacataattattacct                    >  2:212920‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                            aaaaGAGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttg                >  2:419171‑M1/1‑34 (MQ=255)                                                                                                                                                                                                                                         tCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattaccttttgaagaaattagccct  >  1:419609‑M1/1‑21 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              AAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAATTCGTCCGCAACGTGGCAG  >  NC_000913/1628087‑1628569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑