Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,861	A→G	intergenic (+79/+6)	C0293 → / ← ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,861	0	A	G	100.0%	40.4 / NA	12	intergenic (+79/+6)	C0293/ymfD	small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

GTTAGTTATGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAA  >  NC_000913/1196619‑1197018                                                                                                                                                                                                                                                   |                                                                                                                                                              gttagttaTGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAcc                                                                                                                                                        >  1:331597‑M1/1‑248 (MQ=255)          gTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgatt                                                                                                                                              <  1:188068‑M1/251‑13 (MQ=255)                           gACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAAGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgc                                                                                                                             >  1:448747‑M1/1‑222 (MQ=255)                                               ccTTACCCCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatg                                                                                                          <  2:331597‑M1/250‑49 (MQ=255)                                               ccTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggat                                                                                                           <  1:159183‑M1/249‑48 (MQ=255)                                                            cAAATATTGTTAAATTGCCGGTTTTGTATCGACTACTCACCCGGTACTCGCCAGGGGACAGCCAACAGGCAATGGGCGCAATCGCCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCGCATACATCTTTAGTACTGAGAATTTTTAACCTAGGGAATGATTGTCGTAccatcaacgattaaactgcgcttcggcgcaattttcgtttacaggatgttgaaagttaaaat                                                                                            <  2:326323‑M1/251‑63 (MQ=255)                                                                       aaaTTGCCGGTTTTATATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACAGACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTGccatcaacgattaaacttcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaa                                                                                 <  1:416531‑M1/251‑74 (MQ=255)                                                                                gTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGAAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATAATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccg                                                                        >  1:178756‑M1/1‑169 (MQ=255)                                                                                          aaCTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacgg                                                              >  2:451859‑M1/1‑159 (MQ=255)                                                                                                 cACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggga                                                        >  1:16142‑M1/1‑152 (MQ=255)                                                                                                                         ccAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaaacccatcctataggagaacaa                                  >  1:316069‑M1/1‑128 (MQ=255)                                                                                                                                                      cGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTGTCGTAccatcaacgattaaactgcgcttcggcgcagttttcgtttacaggatgttgaaagggaaaattctggggcaaaaaaagcccgccagttacggcgggaaacacccacctatgggggaacaatgaaaatagaaattgcgggggtatgatttccc  >  2:23172‑M1/1‑99 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                              GTTAGTTATGTATTCACTGTTATTAGGACAATTATGAATTACCACTCCTTACACCCGCTCAAATATTGTTAAATTGCCGGTTTTGTATCAACTACTCACCCGGGACTCGCCAGGGGACAGCCAACAGGCATTGGGTGCAATCACCTTAGCGTTCAGGTACATGCGGAATGTAAAAAAGGCCGCGAGCGCGGCCCCTTCACATACATCTTTAGTACTGAGACTGTTTAACCTAGGGAATTATTATCGTATTATATTGCATTACAACTCCCAACAGTGACATATGAACTTCCTGCTTTACTCCACGACTTTAATATTTCAAGGCCATGTGAAGAACATATTTCTTGCACTGCGAGTTCATCGAGTAATCCATAGTAAGAAACACTTTTTGAATTTTTGTAAA  >  NC_000913/1196619‑1197018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑