Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,518	G→A	A89V (GCG→GTG)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,518	0	G	A	100.0%	177.2 / NA	53	A89V (GCG→GTG)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

TTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCG  >  NC_000913/674281‑674764                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       ttcgCCAGTACGGTCTGGTCGTTCGGGCCCCAGTTGACCGAAGAAGTCTTCTTATATACCAGGCCTTTTTTATACATCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCTTACGTCCGCGGTGCCGGAGGGGTGTTGTTTTTCACCGCCACGCCTTCCgcag                                                                                                                                                                                                                                            <  1:167998/250‑1 (MQ=255)   cgCCTGTACTGTCTGGTCGTTCGGGCCCGAGTTGACCGCAGAAGTTTTTTTATATACCAGCCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTTTTCCCAACGGTAGTATTCGGGCGTACAGTTGGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTCCGCCCACGGTTCCGGGGCGGTTTTTTTTTTCACCGCCACGCCTTCCgcaggc                                                                                                                                                                                                                                          <  2:254199/250‑1 (MQ=255)            ggTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcg                                                                                                                                                                                                                                >  1:106825/1‑251 (MQ=255)                tgttCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGG‑TGCAGAATTTCTGTTCCCAACGGTAGTCTTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTTCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcgcctc                                                                                                                                                                                                                            >  2:384446/4‑248 (MQ=255)                   tCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTccc                                                                                                                                                                                                                          >  1:52472/1‑250 (MQ=255)                        cGGGCACCAGTGGCCCGCAAAGGTTTTCTTATACCCCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTGGCCGGCTCGCGGCTCCA‑GTCATAACAAAAGCCCAGCATTTTGAGCTGGTTTTTCATATCCGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCg                                                                                                                                                                                                                     <  2:52472/250‑1 (MQ=255)                                           aaaGTCTTCTAATAGACCAGGC‑TTTTTTTTACCGGTGGGGTGAAGAATTTCTGTCCCCAAGGGTAGGTTTCCGGGTTACAGGTTCCCGGCTCGCGGCCCCA‑GCCATACCCAAAGCCCGGCATTTGGGGCGGGTTTTTCATATACGCGATTTTTTCGTCCGTCCCCGGTGCCGGAGCGGTTTTGTTTTCCTCCGCCACGCCTTCCGCAGGCATACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGttttt                                                                                                                                                                                                 <  2:98002/250‑1 (MQ=255)                                            aaGTTTTCTTATAAACCGGGCCTTTTTTATACGGCTCGG‑TGAAGAATTTCTGTCCCCAAGGGTGGTATTCCGGGGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTGGTCGTACGTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTg                                                                                                                                                                                                <  2:310109/251‑1 (MQ=255)                                                  tcttatatcactggctttttttATACCGCTCGG‑GGAAAATTTTCTGTACCCAACGGTAGTATCCCGGCGGACGGGTTGCCAGCCCGCGGCTCCA‑GTTATACCAAAAGCCCAGCATTTTGGGCTGGTTTTTCATATACGCGATGTGGTCGTCCGTCCACGGTGCCGGAGCGGGGTTGTTTTCCACCGCCACGCCTTCCGCAGGCATACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAg                                                                                                                                                                                           <  2:460895/234‑1 (MQ=255)                                                  tcttATATACCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGccc                                                                                                                                                                                             <  1:99264/248‑1 (MQ=255)                                                       tataCCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCCGGCCGTTTTTGccgcgcctacg                                                                                                                                                                                     >  2:159925/1‑242 (MQ=255)                                                              ggCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAg                                                                                                                                                                              <  1:483235/251‑1 (MQ=255)                                                                 cTTTTTTATACAGCCCGG‑CGAAGAATTTCTGTTCCCAACGATAGTATTCATGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAACGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCAGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCCGGACGGTTTTGCCCAGCATACGCTGGAAGCgg                                                                                                                                                                           >  2:146018/1‑251 (MQ=255)                                                                   tttttATACAGCCCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGTTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCggg                                                                                                                                                                          <  1:211562/250‑1 (MQ=255)                                                                     ttaataCAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCAAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGGTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTTCCGGACGCTTTTGCCCAGCAGACGCAGTTAGCGGGCg                                                                                                                                                                        >  2:257323/4‑250 (MQ=255)                                                                       tataCAGCTCGG‑TGCAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATc                                                                                                                                                                     >  2:398582/1‑251 (MQ=255)                                                                           cAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGt                                                                                                                                                                 >  1:300176/1‑251 (MQ=255)                                                                            aGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTc                                                                                                                                                                >  1:424426/1‑251 (MQ=255)                                                                                       agaaTTTCTGTTCCCAACGGTAGTATTCCTGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGTCGATCACGTCACCGATGgtg                                                                                                                                                      >  1:288539/1‑251 (MQ=255)                                                                                        gattttCTGTTCCCAACGGTAGTATTCCGGCGTCCAGGTGGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGTTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtgt                                                                                                                                                     <  1:182323/248‑1 (MQ=255)                                                                                        gaaTTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGCTCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGgtgt                                                                                                                                                     >  2:107897/1‑251 (MQ=255)                                                                                         aaTTTTTGTTCCAAAGGGTATTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATACCCAAAGCCCAGCTTTTTGAGCTGTTTTTTCATATTCGCGATGTTTCCGTCCGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTa                                                                                                                                                    <  1:376734/251‑1 (MQ=255)                                                                                          aTTTCTGTTCCCAACGGTAGAATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATATTGTCGTACGACCACGTTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCCGCCGATCTGATGCAGGCCGTTTTTGCCCCGCATACGCTGGTCGTGGGCGATCACGTCAcctcgtgtgt                                                                                                                                                     >  2:70537/1‑241 (MQ=255)                                                                                            ttCTGCTCCCAACGGTAAAAATACGCCGTACAGGTTGCCAACTCGAAGCTCCA‑GTCATAACCAAAGCCCAGACTTTTGCGCTGGTTTTTCATATACGCGCTGTTGTCGTACGTCCACGGTGCCGGAGCGGAGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCaacactggagtagtt                                                                                                                                                 >  2:138831/1‑237 (MQ=255)                                                                                             tcttttCCCAACGTTATTATTCCGGCTTACAGGTTCCCAGCCCGCGGCCCCA‑TCCATAACCAAAGCCCAGCATGTTGATCTGGTTTTTCATATACGCGATGTTGTCTTCCGTCCACGTTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGtt                                                                                                                                                 <  1:296759/246‑1 (MQ=255)                                                                                                      aaCGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTtacgtacgggg                                                                                                                                       >  1:20469/1‑248 (MQ=255)                                                                                                         ggTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGGGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGTTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTGTTTGCCCAGCATACGCTGGTAGAGGGCGCTCACGTCTCCGCTGGTGTAGTTACGTACGTGAccc                                                                                                                                    >  2:3830/1‑251 (MQ=255)                                                                                                         ggTAGTATTCCGGCGTAAGGGTTGCCAGCTCGCGGCTCCA‑GCCAAAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTCCGTCCACGGTGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGccc                                                                                                                                    <  1:395930/251‑1 (MQ=255)                                                                                                                    ggcgtaagggTTGCCAGCCCGCGGCTCCA‑GTCATAACCAAACCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCCCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCg                                                                                                                         <  1:337791/243‑1 (MQ=255)                                                                                                                      cGTACAGTTGGCCAGCTCGCGG‑TCCACTTCATAACCAACGCCCATCTTTTTGAGTTGTTTTTTCAGATACGTGTTGTTTTCTTGCGTCCCGGGTGCCGGGGGGGTTTGGTTTTCCACCTCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACAGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAc                                                                                                                       <  2:318935/251‑1 (MQ=255)                                                                                                                         aCAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGAcc                                                                                                                      >  1:164347/1‑249 (MQ=255)                                                                                                                             gtggCCAGCTCGCGGCTCCA‑GTCAGAACCAAAGCCCAGCATTTTGAGCGGGTTTTTCATAAACGCGATGTTGTCGTACTTCCACGGTGCCGGGGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCAcc                                                                                                                                                             <  1:415344/203‑1 (MQ=255)                                                                                                                             gTTGACAGATCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGCGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAACCGCGTCCCAGCCGATCGGCTGCCGGACGTTTTTGCCCAGCATACTCTGGTAGCGGGCGATCCCGTCAcc                                                                                                                                                             >  2:415344/1‑206 (MQ=255)                                                                                                                                   aGCTCGCGGCTCCA‑GTCATAACCAACGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGCAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCCGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAggg                                                                                                          >  1:133936/1‑251 (MQ=255)                                                                                                                                        gcgGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGc                                                                                                      >  1:386497/1‑250 (MQ=255)                                                                                                                                           gCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCAt                                                                                                    >  1:213764/1‑249 (MQ=255)                                                                                                                                                      tAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGt                                                                                         >  1:300808/1‑250 (MQ=255)                                                                                                                                                           aaaGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACt                                                                                   >  1:68281/1‑251 (MQ=255)                                                                                                                                                                         tGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGCCCGCGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCtctc                                                                      <  1:442592/250‑1 (MQ=255)                                                                                                                                                                          gAGCTGTTTTTTCATATACGCTATGTTTTCGTACGCCCACGGTGCCGGAGCGGTGTTTTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGt                                                                    <  1:369994/251‑1 (MQ=255)                                                                                                                                                                           aGCTGGTTTTTCATTTCGGCGTTGTTGGCGTCCTGCCAGGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCTGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTc                                                                   <  1:230326/251‑1 (MQ=255)                                                                                                                                                                               ggTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGTTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCg                                                               <  1:460039/251‑1 (MQ=255)                                                                                                                                                                                             gcgATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCCCCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAAtgg                                                 >  2:402711/1‑250 (MQ=255)                                                                                                                                                                                                    gggggaccggccgggtgggcggtgCGGTGTTGTGTTCCTCCGCTACGCATTCCGAGGGCAGACCAAACGCGCCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGTAGGATAGGGAAGCATAGACAGGCAGTAATACTTCGCTTTGTTCTCGTCTGCGGTTACTTTAAATGTGCGCtt                                           <  2:228154/227‑1 (MQ=255)                                                                                                                                                                                                      tCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGAAGTTTTTGCCCAGCATACGATGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtctc                                        >  1:318758/1‑251 (MQ=255)                                                                                                                                                                                                      ccgtccGTCCCCGGTGCCGGGGCGGTGTTGTTTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTtctc                                        <  1:177683/246‑1 (MQ=255)                                                                                                                                                                                                              ccgcggtgccggtgcGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAAt                                <  1:257323/248‑1 (MQ=255)                                                                                                                                                                                                              ccacggcgcgggtgggttttgttttttCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGCCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCGTTGCTCTCGTCTTCGGTTACTTCAAACGTGCGCTTCTCATCCCAAt                                <  2:431547/229‑1 (MQ=255)                                                                                                                                                                                                                 cGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGa                              >  1:247894/1‑250 (MQ=255)                                                                                                                                                                                                                       cGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGt                        >  1:357226/1‑250 (MQ=255)                                                                                                                                                                                                                                  tgttTTTCCCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTc             <  1:127192/250‑1 (MQ=255)                                                                                                                                                                                                                                    ttttcCACCGCCACGCCGTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTa           <  2:424426/250‑1 (MQ=255)                                                                                                                                                                                                                                       ttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGATTCTCATCCCAATGACGCGGTACTTTGGATTCTAtctc       >  2:240195/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgccacGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCg  <  1:107897/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       TTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGG‑TGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCA‑GTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTGGTAGCGGGCGATCACGTCACCGATGGTGTAGTTACGTACGTGGCCCATGTGTAGTCGACCAGAAGGATAGGGAAGCATAGACAGGCAGTAATACTTCTCTTTGCTCTCGTCTTCGGTTACTTCAAATGTGCGCTTCTCATCCCAATGAAGCTGTACTTTGGATTCTATCTCTTCCG  >  NC_000913/674281‑674764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑