Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	108.4 / NA	31	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/21);  total (10/21)

GATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATT  >  NC_000913/4312789‑4313269                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   gATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCa                                                                                                                                                                                                                                        <  1:3399/251‑1 (MQ=255)        tAACTGTCATCGCTTCAGATACCGCCAGAGCTTCTGGGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGGTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACGAGCTCACCACATCAAGAACa                                                                                                                                                                                                                                  <  2:97045/250‑1 (MQ=255)        tAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAAc                                                                                                                                                                                                                                   <  1:464538/249‑1 (MQ=255)                     gtCAGATACCGCCAGAGCGACAGCGACATCTTTAAGGGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTGTCTGTCATTCCTGCCTGCTTCATTCGCAGGTAAGGCGCTAACCCGATACCGTTCGGAAGCGCTGAAGCAAACTCTGACTGGCTCATCACGTGCGCGAGTACTTGAATTGAAGACGCCACTAGAGCACCACAGCAAGAACAGAAAAGATGCTGGc                                                                                                                                                                                                                    <  2:103641/250‑1 (MQ=255)                               gcgagagCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTGGAGTAACCACTCCACGAGGCGGCTTTCAGTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCTCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGGTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGTCGACTTCACaa                                                                                                                                                                                                          <  2:93247/248‑1 (MQ=255)                                   gagCTTCTGCGACATCCTTAATTTCGGGTGCACATCTCAGTTTACCGGGTTTGAGTAACCACTCCACGGTGCGTCTGTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCATGTAAGTCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAATCGCTGACTGGCTCATCACGTTCCCTTGGCCTTTAATTGAAGACGCCACTAGATCGCCACAGGAAGAACAGAAAAGATGCTGGCTACTTCACtaaaaa                                                                                                                                                                                                      <  2:138488/251‑1 (MQ=255)                                           gCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAaaaataaaat                                                                                                                                                                                               <  1:418786/250‑1 (MQ=255)                                                aTCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTtata                                                                                                                                                                                          >  1:394141/1‑250 (MQ=255)                                                                  acaACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGaa                                                                                                                                                                       <  2:62128/251‑1 (MQ=255)                                                                                 gggTTTTAGTAACCACTACACGTTGCGGCGTTCCTTTTCTGTCATTACTTCGTGCTTCATTCGCAGGTAAGGCGCTAACCCTATATCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACTTTCTCTTGACCTTGAATTGAAGACACAACTAGATCACCACAGCAAGAACCGAAAAGATGATGGTGACTTCACAAAACAATAAAATTAGTAAAGTTGATTTTATTGTGAACAATGGCGACTGGCt                                                                                                                                                        >  2:132274/1‑251 (MQ=255)                                                                                      tGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTaaa                                                                                                                                                    >  1:217055/1‑250 (MQ=255)                                                                                                        tGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGCAGCGCTGATTCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGCCGCCACTAGCTCCCCACAGCACGAACAGTAAAGATGCTGGAGACTTCACAAAAAAATAAAATTTATATAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAACTTTTATTTCATTTacttt                                                                                                                                 >  2:355263/1‑247 (MQ=255)                                                                                                         gCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTa                                                                                                                                 <  1:85718/250‑1 (MQ=255)                                                                                                            gCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGttt                                                                                                                             <  2:101948/251‑1 (MQ=255)                                                                                                                     ttCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACat                                                                                                                     <  1:440424/250‑1 (MQ=255)                                                                                                                           cATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTa                                                                                                              <  1:20207/251‑1 (MQ=255)                                                                                                                                  tcctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtt                                                                                                        <  1:391398/250‑1 (MQ=255)                                                                                                                                   cctGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGtttt                                                                                                      <  2:217055/251‑1 (MQ=255)                                                                                                                                          cATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGt                                                                                                <  1:16798/250‑1 (MQ=255)                                                                                                                                                       ggCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAg                                                                                  <  2:459294/251‑1 (MQ=255)                                                                                                                                                          gcTAACCCTTTACCGGTCGGCAGCGCTGCATCAAACTCTGACTGGATCATCACGTTCTCTTGACATTGAATTGAAGACGCCACTAGGTCACCACAGCAAGTACAGAAAAAAGGCTGGAAACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCAAGCGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGACGTTATTTGTTTTTCTGGTTTTTTAGATTATAGAt                                                                                >  2:83980/1‑250 (MQ=255)                                                                                                                                                                 cTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTgg                                                                        >  1:488429/1‑251 (MQ=255)                                                                                                                                                                       cGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTg                                                                  <  2:324534/251‑1 (MQ=255)                                                                                                                                                                            ggAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGt                                                                                                                               <  1:361100/185‑1 (MQ=255)                                                                                                                                                                            ggAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGt                                                                                                                               >  2:361100/1‑185 (MQ=255)                                                                                                                                                                              aaGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCt                                                           >  2:499128/1‑251 (MQ=255)                                                                                                                                                                                                 gACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGTGCTg                                          <  2:231145/249‑1 (MQ=255)                                                                                                                                                                                                  aCTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTc                                        >  1:318896/1‑250 (MQ=255)                                                                                                                                                                                                              cGTTCTCTTGTGCGTGAATTGAAGACGCCAATAGATCACCACAGCAAGAACAGAATAGATGCTGGTGACTTCACAAAAAAATGAAATTTATAAAGTTTATTTAATTGTGAACAATGGCGAGTGCCTTAAAATTTTATTTCATGTACTTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTGGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGGAAcgcta                           <  2:47919/251‑5 (MQ=255)                                                                                                                                                                                                               gTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGt                          >  1:407877/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAAtt  <  1:113053/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   GATACCTTAACTATCATCGCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATT  >  NC_000913/4312789‑4313269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑